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Citations for Millipore Sigma :
2551 - 2600 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Cell Biology 2023Quote: ... Hsp60 (1:1000, QED-BIOSCIENCE 11101) or glyceraldehyde-6-phosphate dehydrogenase (rabbit polyclonal anti-G6PDH antibody, 1:10000, Sigma A9521). Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Bioengineering 2024Quote: ... The slides were then washed with 0.1% Tris-buffered saline and Tween-20 (TBST) prior to 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (D9542 Sigma) for nuclei visualization ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS and then seeded in 6-well plates (5×105 cells/well) that had been pre-coated the day before with type I collagen (Sigma) dissolved in 0.02 N acetic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... dissociated to single cell suspensions and cultured in RPMI-1640 (complete medium) supplemented with 60 μM 6-Thioguanine (Sigma-Aldrich) as previously described (18 ...
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bioRxiv - Cancer Biology 2023Quote: Bone marrow cells were acquired by flushing the femurs and tibias of 6- to 8-week-old female BALB/c mice and treated with red blood cell lysis buffer (Sigma). Lineage negative hematopoietic stem and progenitor cells (HSPCs ...
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bioRxiv - Physiology 2024Quote: ... followed by rehydration with different % of ethanol (From 100 % to 70 %, 111000190, Pharmco) and the antigen retrieval step with citric buffer (pH 6, C9999, Sigma) in heat for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... Strainers containing fRSS-washed cells were further washed using 500 µL of freshly prepared 6% sodium hypochlorite (Reagent Grade, Sigma) to remove SAB ...
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bioRxiv - Systems Biology 2024Quote: ... Reporter cell lines (100-150 million cells) were transduced with packaged retrovirus in the presence of 6 µg/ml polybrene (Sigma) and at low transduction efficiency (< 20% ...
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bioRxiv - Systems Biology 2024Quote: ... seven-week-old female C57Bl/6 mice were treated with oral administration of CCl4 (Sigma-Aldrich, St. Louis, MO, USA) diluted in olive oil ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the NPSH levels in the samples (50 µg of protein) were mixture of 50 µL of 6% TCA (Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Expanded colonies were tested by inducing ER stress with 200 nM thapsigargin for 6 h (Sigma-Aldrich, Burlington, MA, USA) using confocal microscopy (Zeiss LSM800 controlled by the ZEN 2.3 software ...
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bioRxiv - Cell Biology 2024Quote: ... the respective secondary antibodies were incubated in ADB at room temperature in the dark for 90 min together with 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich). Before imaging the cells were washed again using PBS ...
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... samples were washed in 1X PBS and incubated with DAPI (4-6-diamidino-2-phenylindole, Sigma-Aldrich, 1 µg/mL) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Plant Biology 2024Quote: ... 6 ml of the suspension was equally distributed and loaded on top of four continuous Percoll (Sigma Aldrich, Heidelberg, Germany) gradients (containing 50% Percoll / 0.4 M sorbitol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 30 minutes on ice with 6 nm (Jackson#711-195-152) or 5 nm (Sigma#G7277) diameter immunogold coupled secondary antibodies in DMEM-FBS-HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... were treated with 50 µL of a 1mg/ml Concanavalin A (prepared in 10 mM sodium phosphate buffer pH 6, 10 mM CaCl2 ,1 mM MnCl2, 0.01% NaN3, Catalog C2010, Sigma Aldrich) solution ...
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bioRxiv - Systems Biology 2024Quote: ... Routine cells passaging was carried out at a 1:6 ratio every 2 days using 1× Trypsin–EDTA solution (Sigma) at 37°C ...
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bioRxiv - Physiology 2024Quote: ... slides were incubated at room temperature for 6 hours in staining solution containing 15 mM citrate buffer pH 5.3 (Sigma Aldrich), 2.8 mM CuSO (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: LUHMES-derived DA neurons (day 6 of differentiation) were treated with a combination of antimycin A (25 µM, Sigma-Aldrich), and oligomycin (10µM ...
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bioRxiv - Biochemistry 2024Quote: A DNA consisting of 2 base pairs-Widom 601 sequence (145 base pairs)-2 base pairs was amplified by PCR using a 5’-/6-FAM forward primer (Sigma). The PCR products were pooled from four 96-well PCR plates (100 µL per well ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mL of MSC supplement and 6 mL of LAlanyl-L-Glutamine (ATCC, PCS-500-040) and 0.2 mg/mL G418 (Sigma, G8168) were included as medium supplements ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated in in 24-or 6-well plates previously coated with Poly-L-ornithin (0,1 mg/ml, Sigma-Aldrich) at a density of 250,000 cells/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Mice were acclimated to an isolated behavior space for 1hr prior to gavage with 100µl of sterile carmine red dye (6% w/v) (Sigma, C1022) dissolved in 0.5% methylcellulose (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Hyde) were cultured for 4 days at 37ºC in BSK-II media and complete with 6% rabbit serum (Millipore Sigma) at pH of 7.6 ...
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bioRxiv - Cell Biology 2024Quote: ... the cell layers were washed with 200 µL of 6 M Guadinium Hydrochloride (Gdn-HCl, Sigma, Cat no. G3272-25G), 5 mM EDTA (Fluka ...
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bioRxiv - Microbiology 2024Quote: ... the nanoparticles were washed by centrifugation at 20,000 g once and then re-dispersed in 2 mL of a 6 mM sodium citrate solution (Sigma-Aldrich). Next ...
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bioRxiv - Immunology 2024Quote: ... each microfluidic device was filled with 60 µL of a hydrogel formulation containing the following components: 6 µL of 10x phosphate-buffered saline (PBS) (#5493, Sigma), 6 µL of 0.1 M sodium hydroxide (NaOH ...
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bioRxiv - Molecular Biology 2024Quote: ... HRS/Gsc-GFP dual reporter cells at day 6 of the ADE differentiation were isolated by washing the cells twice with PBS (Sigma; suitable for cell culture ...
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... After 23 h, cells were fixed with 4% paraformaldehyde and stained with DAPI (4’, 6-diamidino-2-phenylindole) (Sigma-Aldrich, USA). Nine fields per well were imaged on an Operetta high-content imaging system (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... Mia PaCa-2 cells and PCAFs were then transduced with the respective viral suspension at different multiplicity of infection (MOI) in the presence of 6 µg mL-1 hexadimethrine bromide (polybrene, Sigma H9268). Cells were sorted for mCherry and EGFP fluorescence using a BD FACSAria™ Cell Sorter (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... 150,000 cells were stained with 10 μM 4’-(trimethylammonio)-diphenylhexatriene (TMA-DPH) or 2 μM Laurdan (6-dodecanoyl-N,N-dimethyl-2-naphthylamine; both obtained from Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... the neurons of postnatal day 1–2 NMRI strain mice were grown 6 DIV on polyornithine-laminin (P3655 and CC095, Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141 ...
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bioRxiv - Developmental Biology 2021Quote: ... testes were incubated at 32 °C for 25 min in 6 mL of Gey’s Balanced Salt Solution (GBSS; Sigma-Aldrich G9779) containing 1.2 mg/mL of Collagenase Type I (Sigma-Aldrich C0130) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... Tissues submerged in RNAlater solution were mixed with 2 ml of 6% (w/v) betaine (Sigma Aldrich, St. Louis, MO, USA) and placed on ice prior to crushing with mortar and pestle until gut homogenates were formed ...
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bioRxiv - Immunology 2021Quote: ... At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich). Supernatants were collected at 24 and 48 hrs and were subsequently combined and filtered through a 45 μm vacuum filter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The plates were incubated for two hours at RT and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate solution (Sigma-Aldrich, USA) was added for colour development ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine staining was performed on spleen and LN single cell suspensions following 5-6 hr stimulation with PMA (10ng/ml) (Sigma Aldrich) and ionomycin (1μg/ml ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Developmental Biology 2020Quote: For virus titration in a relevant cell type, neural NE4C cells (ATCC, CRL2925™) were cultured on a 6-well plate in MEM (M5650, Sigma) supplemented with 10% FBS and 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2020Quote: ... The embeded tissue were then sectioned with the thickness of 6 μm and treated with post-fix medium (3.7% formaldehyde (Sigma-Aldrich, 252549) and 0.1% DEPC (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... 6×105 cells were infected in 6-well plates with 125uL of high titer virus in standard media with 5ug/mL Polybrene (Sigma-Aldrich). Media was changed after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... A21111) Slides were counterstained using 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL in PBS, Sigma-Aldrich, cat. D9542-5MG). Coverslips were mounted using mounting medium (Fluoromount-G™ Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... Each well of 6-well cell culture treated plates coated with 0.1% Poly-L-ornithine (3 ug/mL; Sigma Aldrich, P4957) were seeded with ∼750K cells and maintained with sterile-filtered neurobasal medium (Thermo Fisher Scientific ...