Labshake search
Citations for Millipore Sigma :
2501 - 2550 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... centrifuged and the cell pellet was lysed using a Lysis Buffer (25 mM Tris pH 7.6, 150 mM NaCl, 1% NP-40, 1% Na-deoxycholate, and 0/1% SDS in water + protease inhibitor cocktail [Sigma] + phosphatase inhibitor cocktail [Sigma-Aldrich] + okadaic acid + sodium fluoride) ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with ice-cold PBS and incubated with 0.25 µg/mL biotin hydrazide (66640-86-6, Sigma-Aldrich) in cold PBS for 1 hour on ice ...
-
bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
-
bioRxiv - Immunology 2022Quote: ... were administered to C57BL/6 mice by subcutaneous (SC) injections on days 0 and 28 in the presence of Sigma adjuvant system (Sigma). 2 µg of S and S-EABR mRNA-LNP were administered to BALB/c mice by intramuscular (IM ...
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were plated at a density of 8x10^6 cells per 10 cm dish coated with 60 μg/ml poly-D-lysine (Sigma) and 2.5 μg/ml laminin (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... Disposable PS plastic was readily available as the flat bottom of a 6-well tissue culture plate (TPP®, Sigma). Disposable glass cover slides (22CIR-1 ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were mounted in Sigma-Aldrich DuoLink in situ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich), allowed to incubate at room temperature (RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3533-005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma, CLS3471). H2B:GFP retrovirus (91 ...
-
bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
-
bioRxiv - Genomics 2023Quote: C57BL/6 mouse ESC were purchased from ATCC (SCRC1002) and maintained onto 1% gelatin-coated (Sigma-Aldrich, Cat. No G1393) dishes in DMEM knockout medium (Life Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Monocytes were then isolated with CD14+ microbeads (MACS Miltenyi) and incubated for 6 days in RPMI/10%FBS and 1% penicillin/streptomycin solution (Sigma) supplemented with 50 ng/mL human GM-CSF (R&D Systems ...
-
bioRxiv - Immunology 2023Quote: ... 0.4 × 106 CXCR3A-transfected CHO cells/ml or 60 000 HMVEC/ml (2ml/well) were seeded in flat bottom 6-well plates (2.0 ml/well; TPP, Sigma-Aldrich) in supplemented Ham’s F-12 growth medium + 10% (v/v ...
-
bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1-1.2x106 cells/ml onto 6-well plates treated with poly-D-lysine (EMD Millipore). Cells were cultured in Neurobasal A medium supplemented with 2% B27 ...
-
Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
-
bioRxiv - Bioengineering 2023Quote: ... 3D collagen matrix systems were stained with 50 µM of 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE, Sigma-Aldrich) at room temperature for 60 minutes and visualized using a confocal laser scanning microscope (cLSM ...
-
bioRxiv - Microbiology 2023Quote: ... a dilution series of 107 to 103 cells/ml were plated in 5 μl spots on M9 minimal media plates with 10 mM glucose or 10 mM glucose-6-phosphate (Sigma) and antibiotics ...
-
bioRxiv - Neuroscience 2023Quote: ... Mice fasted overnight were gavage fed 6% carmine red dye (300 µl) Sigma-Aldrich) in distilled water containing 0.5% methylcellulose (Sigma-Aldrich) and placed in individual cages with white paper covering cage bottoms ...
-
bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
-
bioRxiv - Microbiology 2023Quote: ... quenched with 25 mM ammonium chloride in PBS for 10 min and nuclei were stained with 4’,6-diamidino-2-phenylindol (DAPI, Sigma, cat ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK cells were washed three times with ice-cold PBSx1 and lysed using Maltoside lysis buffer [1% N-dodecyl maltoside (cat no. 69227-93-6 – Sigma) dissolved in PBSx1 with freshly added 0.1 mg/ml PMSF and protease inhibitors] ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 30min at room temperature (10µg/mL, D9542, Sigma-Aldrich). Coverslips were mounted with ProLong Gold Antifade (P36934 ...
-
bioRxiv - Genomics 2023Quote: ... or cell type (roughly 5X106 iPSC-NPCs or 1X106 iPSC-neurons on a 6-well plate, enzymatically detached with Accutase (Millipore) and pelleted with centrifugation for 5 mins at 1,000 G) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
-
bioRxiv - Plant Biology 2023Quote: ... and 2 mL of acid ninhydrin (glacial acetic acid, 6 M phosphoric acid, ninhydrin (2,2-dihydroxyindane-1,3-dione, Sigma, ON, Canada) were boiled in a water bath at 100 °C for 1 hr ...
-
bioRxiv - Cell Biology 2023Quote: ... 3 – 6 mg of cell extract and 20 μl of α-Flag M2 affinity bead suspension (Sigma, catalog no.: A2220) were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
-
bioRxiv - Immunology 2023Quote: 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to exclude dead cells and debris (Sigma Aldrich, St. Louis, MO). All antibodies are from Biolegend ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1×106 cells were plated on one well of 6 well-plate previously coated with 10μg/ml of laminin (Sigma, #L2020) in 1xPBS for 1 hour at 37C ...
-
bioRxiv - Genetics 2023Quote: ... Pellets of ∼6 x 108 cells were used to extract genomic DNA using acid-washed beads (Sigma-Aldrich, G8772-100G) and phenol-chloroform (Thermo Scientific) ...
-
bioRxiv - Developmental Biology 2023Quote: ... dissected animal caps from injected and uninjected sibling embryos were cultured in 1X Steinberg’s solution for 6 hours 53 in the presence of 10 ng/ml activin (Sigma, A4941) and 1X 10-4 M retinoic acid (RA ...
-
bioRxiv - Cell Biology 2023Quote: ... Gene mutation was induced 4 days after organoid passage during 6 days with 400 ng/ml doxycycline (D9891, Sigma-Aldrich). When indicated ...
-
THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon tissues from one wild type and one Olfr78-GFP KO mice (respectively 8 and 6 months-old) were cut in small pieces and digested with 0.5 mg/ml Collagenase I (Sigma Aldrich) for 25 min at 37°C under agitation at 75 rpm ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed with PBS and the cells’ nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL, Sigma-Aldrich) for 5 min at room temperature in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... Debris was removed by 2 successive centrifugations (6 minutes at 600 rpm) and cells were plated onto coverslips coated with 0.01% poly-L-ornithine (Sigma, P4957) and incubated overnight at 37 °C with 5% CO2 in serum-free DMEM.
-
bioRxiv - Neuroscience 2023Quote: ... Induced neurons were replated onto 6-well tissue culture plates at 450k cells/well on pre-coated with poly-L-orthnithine (Sigma), 4 μg/ml Laminin (Roche Basel ...
-
bioRxiv - Neuroscience 2024Quote: Pharmacological experiments were carried out on PCs during simultaneous somatic and dendritic recordings after 10 minutes of control recording using ACSF with the following drugs: 20 µM 4- (N-ethyl-N-phenylamino)-1,2 dimethyl-6-(methylamino)pyrimidinium chloride (ZD7288) (Sigma-Aldrich), or 1 µM TTX ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... mice were fasted for 6 h and received an oral (2 g/kg body weight) glucose load (G7021, Sigma Aldrich). For the insulin tolerance test ...
-
bioRxiv - Systems Biology 2024Quote: ... About 75% of confluent cells were harvested and rinsed with 6 ml Dulbecco’s Phosphate-buffered Saline (DBPS) (Sigma Aldrich, USA). The cells were then trypsinized with 2 ml of 1 X trypsin and were incubated for 45 s (HEK 293 ...
-
bioRxiv - Microbiology 2023Quote: ... or CEM were infected overnight in a 6-well plate in the presence of 0.5 μg/mL polybrene (Sigma-Aldrich). The following day ...
-
bioRxiv - Molecular Biology 2023Quote: The resulting oxidized DNA samples (∼300ng) were mixed with 20 mM phosphate buffer (pH 6) and 5.1 mM (+)-biotinamidohexanoic acid hydrazide (Sigma-Aldrich), and incubated at 40°C for 4 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... and the AMPA receptor inhibitor 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 µM, Sigma Aldrich, St Louis, MO, USA). This solution was applied to the autaptic culture neurons for 2 min ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein was eluted from the resin by applying 6 mL of wash buffer supplemented with 20 mM reduced glutathione (Sigma).
-
bioRxiv - Biochemistry 2023Quote: ... Eluted and purified mGlu5-5M receptor and mGlu5-5M_W785A receptor mutant bound to quisqualate and PAM were concentrated to 5-6 mg/ml using Amicon Ultra-4 centrifugal concentrator 100 kDa (Millipore) for cryoEM grid preparation and analysis.
-
bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
-
bioRxiv - Biophysics 2023Quote: ... 1.5 – 2 million cells were plated on a 6-well-plate coated with 10 µg/ml fibronectin (Sigma; F1141-5MG) and maintained in IMDM (GlutaMAX ...
-
bioRxiv - Bioengineering 2023Quote: ... The resulting submucosa was cut into 6 inch pieces and decellularized using 4% alcohol/0.1% peracetic acid (v/v, Sigma-Aldrich) and washed thoroughly with 1X PBS and Type 1 water followed by lyophilization and comminuted into an injectable particulate form via cryogenic milling and sieving through 425 µM pore sieve ...
-
bioRxiv - Cancer Biology 2023Quote: ... 6-week-old ElaK mice were first treated with tamoxifen (T5648-1G, Sigma Aldrich. 30 mg/ml in corn oil) by oral gavage and 4-hydroxytamoxifen (H7904-25mg ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were seeded in 6 cm dishes one day prior to treatment with 1 μg/ml actinomycin D (Sigma-Aldrich) for the indicated time ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in staining media supplemented with 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from analyses ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Sigma) to eliminate dead cells from sorts and analyses ...