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Citations for Millipore Sigma :
2501 - 2550 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and Endothelin-1) using a fluorometric MILLIPLEX ® Human Angiogenesis/Growth Factor Magnetic Bead Panel Multiplex Assay (Sigma-Aldrich, Cat # HAGP1MAG). Each plate was run according to the manufacturer’s protocol using 25 µL sample per well.
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bioRxiv - Synthetic Biology 2023Quote: ... 8 µL of 300 µM final concentration of substrate diluted in TVMV protease assay buffer (50 mM Tris/HCl, 100 mM NaCl, and 2 mM DTT, 50 mg mL-1 BSA (Sigma-Aldrich), pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: We quantified the T7 phage titers extracted from the washed and unwashed pipette tips using the standard double-layer plaque assay (T7Select® System Manual, Novagen). A single colony of BL21 bacteria was grown in 4 ml M9LB agar media overnight at 37°C while shaking at 250 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... glutathione levels in biological samples were measured using Ellman’s Reagent and glutathione reductase using Glutathione GSH/GSSG Assay (Sigma-Aldrich, #MAK440) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: We assessed mitochondrial activity using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay (Sigma-Aldrich, Cat# M6494). As a tetrazolium dye ...
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bioRxiv - Neuroscience 2023Quote: ... buffer (for Sanger sequencing and T7 assays) or 1X sodium borate (SB) buffer (for genotyping) with 1.5 mg/mL ethidium bromide (Sigma-Aldrich®). Samples were run alongside 5 μL of 1kb Plus DNA Ladder (New England Biolabs® ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Microbiology 2023Quote: ... aeruginosa cytotoxicity on A549 cells was assayed using a colorimetric assay that detects the number of metabolically active eukaryotic cells able to cleave the MTT tetrazolium salt (Sigma-Aldrich) to the insoluble formazan dye ...
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bioRxiv - Synthetic Biology 2022Quote: ... The buffer of the combined flowthrough was exchanged into assay buffer (20 mM sodium phosphate, 20 mM sodium chloride, pH 8) using a G25 (Sigma-Aldrich) column ...
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bioRxiv - Physiology 2023Quote: ... The resulting crude mitochondrial pellet fraction was resuspended in MIB and protein concentration was quantified with Bradford assay (Sigma-Aldrich, B6916) using a spectrophotometer (Infinite 200 Pro ...
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bioRxiv - Biochemistry 2023Quote: Reporter silencing assays were conducted as in ref 12 with the following changes: cells were treated with 1ug ml−1 dox (Sigma-Aldrich), and gates were set based on reference reporter cells cultured in parallel without dox and reference fully silenced reporter cells (See gating scheme in Figure S2).
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bioRxiv - Molecular Biology 2023Quote: Harvested cells were lysed in radioimmunoprecipitation assay (RIPA) buffer with the addition of protease inhibitor cocktail (Sigma-Aldrich, Cat. No. P83490), briefly sonicated ...
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bioRxiv - Bioengineering 2023Quote: ... Levels of inflammation-associated cytokines were assessed by MILLIPLEX MAP Porcine Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assay (Millipore Sigma), following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Samples were raised to a final volume of 50 μL with Triglyceride Assay Buffer (CAS# MAK266, Sigma Aldrich, St. Louis, MO). 2 μL of Lipase was added to each sample and was incubated for 20 minutes at room temperature to convert triglyceride to glycerol and fatty acids with the plate shaken manually every ten minutes ...
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bioRxiv - Molecular Biology 2023Quote: LX-2 cells were lysed and proteins were extracted using radioimmunoprecipitation assay buffer supplemented with complete EDTA-free protease inhibitor cocktail (Sigma-Aldrich). After centrifugation of cell lysates at 12,000 x g for 10 min ...
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bioRxiv - Cell Biology 2023Quote: Proximity Ligation Assay (PLA) was performed in MCF10A cells using the DuoLink® in situ Orange detection reagent (DUO92102, Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... supernatant of cells was collected and used for quantitating levels of nitrite and nitrate using a Nitrite/Nitrate Assay (Sigma Aldrich) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... The final part portion of the lysate was used to quantify the collagen content according to a hydroxyproline assay (Sigma-Aldrich). The absorbance was measured at 570 nm and the collagen content in the sample was determined from a hydroxyproline standard.
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bioRxiv - Immunology 2023Quote: ... Infected and mock controls were washed three times in PBS and lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Millipore 20-188) at 2 x with protease inhibitor for western blot analysis.
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bioRxiv - Cell Biology 2022Quote: ... medium was changed to pre-mixed treatments in lipolysis assay medium: insulin 100 nM (Humulin-R U-100, Lilly) and/or isoproterenol 1 mM (Sigma-Aldrich). Lipolysis was measured over 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The sample was spun down at 16,873 x g for 10 min and supernatant was diluted to 2 mg/ml after quantification by Bradford assay (Sigma Aldrich). The sample was reduced for 30 min by adding DTT to a concentration of 5 mM followed by an alkylation step with acrylamide at 15 mM for 30 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... For phage dilutions in plaque assays we used an SM buffer supplemented with 10 mM calcium chloride and magnesium sulphate (Sigma-Aldrich). We routinely stored phages as filter-sterilised (0.22 μm ...
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bioRxiv - Physiology 2023Quote: ... Samples of this amniotic fluid supernatant were submitted to the ONPRC/OHSU Endocrine Technologies Core (ETC) to set up bead-based multiplex assays to analyze multiple cytokine and chemokine biomarkers using the Luminex technology (Millipore Sigma).
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bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
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bioRxiv - Cell Biology 2023Quote: ... determined by BCA assay were precleared for 1 hour with Protein G Sepharose 4 Fast Flow beads (GE17-0168-01, Millipore Sigma) at 4°C ...
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bioRxiv - Physiology 2023Quote: ... Analysis of the muscle damage marker creatine kinase (CK) was quantified using spectrophotometry using a coupled enzyme-linked reaction assay (Sigma-Aldrich). Samples were measured in duplicate ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in MA media immediately prior to experiments (FCCP 1μM; Sigma Aldrich cat. C2920).
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bioRxiv - Cancer Biology 2024Quote: ... Mitochondrial lysates for the assay were prepared via dilution of the final isolated mitochondrial suspensions in CelLytic M (Sigma-Aldrich; C2978) at a protein concentration of 2mg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Spheroids were stained with a 10 ml phenol red free assay medium mixture containing 1 μM ethidium bromide (EtBr, E1510, Sigma Aldrich), and 5 μM calcein AM (C3099 ...
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bioRxiv - Microbiology 2024Quote: 30 mg of total protein as determined by Bradford assay of each sample was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich) and mixed by rotation for 2 hours at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The buffer used for assays with purified IgM and IgG fractions was 1% (w/v) casein in 1 x PBS (Sigma-Aldrich) and IgM was detected using an anti-human IgM antibody directly conjugated to HRP (Jackson ImmunoResearch).
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bioRxiv - Biochemistry 2024Quote: 2-MIBS activity assays were performed by pre-incubating samples of 10 to 200 µM GPP (Sigma Aldrich, Cat # 76532-10MG) in a buffer containing 25 µM purified Sg MT ...
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bioRxiv - Biochemistry 2024Quote: ... a general HDAC fluorometric deacetylase enzyme assay was used to determine total HDAC activities relative to wild-type U251-MG cells from various HDAC-immunoprecipitates (Sigma CS1010). To each well of a 384-well plate ...
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bioRxiv - Immunology 2024Quote: PFA-fixed tissue sections were incubated with anti-FLAP and anti-dsDNA antibodies overnight at 4 °C in antibody diluent followed by processing according to manufacturer’s instructions for Duolink® proximity ligation assay (Sigma #DUO92101). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2020Quote: ... The TUNEL protocol was performed using a commercial kit (ApopTag® fluorescein in situ apoptosis detection kit, Millipore). Apoptotic cells were detected as localized bright green cells in a blue background (DAPI ...
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bioRxiv - Neuroscience 2024Quote: ... and some of the myelin was labelled with the green-fluorescent cell linker kit (PKH67 labeling kit, Sigma), according to manufacturer’s specifications.
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bioRxiv - Systems Biology 2023Quote: Leptin - Leptin concentrations were determined by radioimmunoassay using a kit from EMD Millipore (Burlington, MA, kit HL-81K).
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bioRxiv - Molecular Biology 2024Quote: ... we used commercially available ELISA kits: hs-CRP Human C-Reactive Protein ELISA Kit (#RAB0096, Sigma-Aldrich, Germany) and Mercodia Insulin ELISA (#10-1113-01 ...
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bioRxiv - Immunology 2021Quote: ... Gastrin EIA kit was purchased from Sigma-Millipore ...
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bioRxiv - Immunology 2021Quote: ... by using Gastrin EIA kit from Millipore-Sigma.
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bioRxiv - Developmental Biology 2020Quote: ... and processed according to Kit 91A (Sigma) protocol for monocyte detection ...
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bioRxiv - Microbiology 2019Quote: ... iTRAQ® Reagents Multiplex Kit (Sigma Aldrich), Optima™ LC-MS Grade Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2019Quote: ... The λDE3 lysogenization kit was from Novagen. Yeast Extract and tryptone were from Becton Dickinson and Company (MD ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels (Sigma-Aldrich, TrueGel3D Hydrogel Kit, TRUE7) of ∼10kPa were prepared by thawing of the SLO-DEXTRAN solution ...
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bioRxiv - Cancer Biology 2021Quote: ... The Cell Proliferation Kit II XTT (Sigma) was used according to the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s instructions (Alkaline phosphatase Kit, Sigma). The intensity of pink stain reflects the amount of alkaline phosphatase in positive cells.
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bioRxiv - Neuroscience 2020Quote: ... The KAPA Taq PCR kit (Sigma, BK1000) was used in conjunction with three primer sequences (wild type ...