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Citations for Millipore Sigma :
2451 - 2500 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The effect of the anti-angiogenic compounds were measured using the 3-[4,5-dimethylthiazol- 2yl]-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma, Lyon, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell lysates of cultured cells or MDS BM MNCs were prepared with standard radioimmunoprecipitation assay buffer in the presence of protease (Sigma, cOmplete) and phosphatase (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... were loaded in equal amounts across all lanes (based on protein concentration using Bradford assay) and run on 7.5 % acrylamide SDS-PAGE and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Burlington, MA) for 1 h at 100 V at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... ATP to be used for ATPase activity assays was purified after diluting 20 μCi ATP γ-P32 (Perkin-Elmer) in 100 µl of 100 mM unlabeled ATP (Sigma) by passing through a 3 ml P2 resin size-exclusion column equilibrated with a buffer containing 50 mM HEPES·KOH pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: Two immortalized cell lines were used to validate the ACE2 receptor, TMPRSS2 and NRP1 assays: Human oligodendroglioma cells (HOG, Cat# SCC163) were obtained from Millipore Sigma-Aldrich (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells (4×104) were washed twice with PBS and re-suspended with 175 μL of assay medium containing bicarbonate-free RPMI1640 (Sigma-Aldrich) supplemented with 25 mM glucose ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant human IL6 protein had been diluted in assay diluent (PBS with 5 mM EDTA, 100 μg/ml single-stranded salmon sperm DNA (Sigma Aldrich), 0.1% BSA ...
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bioRxiv - Physiology 2022Quote: PKA activity assay was performed using whole cell lysate from iPSC-CMs treated with either PBS or 10μM forskolin (Sigma-Aldrich, F6886) for 30min at 37°C prior to assay ...
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bioRxiv - Cell Biology 2022Quote: ... of SPTAN1-kRas and SPTAN1-Raf1 were identified using the Duolink® proximity ligation assay (PLA) in situ red for mouse/rabbit antibodies (DUO92101, Sigma). THP-1 cells were fixed onto microscope slides and permeabilized as outlined in the ICC/IF section ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transferred to black-walled 96-well plates (Falcon) 4 h before the assay and incubated with EBM or 200nM Staurosporine (Sigma Aldrich) for 4 h ...
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bioRxiv - Bioengineering 2022Quote: ... GAG content in co-cultured constructs was measured using a dimethyl methylene blue (DMMB) assay [79] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Neuroscience 2022Quote: ... United States) according to the manufacturer’s instructions.\Individual assays were performed for total tau (Tau-5, # 577801, Millipore, Australia, 1:50), h-tau (p-Ser202/Thr205-tau (AT8) ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop ...
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bioRxiv - Bioengineering 2022Quote: ... Protein content was measured by the BSA (cells) or microBSA (EVs) protein assay according to the manufacturer’s instructions (Sigma Aldrich, USA).
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bioRxiv - Cancer Biology 2022Quote: ... with 1X protease and phosphatase inhibitor cocktail (Fisher, USA), and 1X DTT (Fisher, USA) and was quantified via a bicinchoninic acid assay (Sigma, USA). The lysates were then separated using SDS–PAGE and electroblotted onto polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice in cold sterile phosphate-buffered saline (PBS) and lysated using ice-cold radio-immunoprecipitation assay (RIPA) buffer supplemented with 1% protease inhibitor cocktail (Sigma-Aldrich) and 1% phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... this TREx/ItypOrco-expressing cell line was tested in a fluorescent calcium assay (described below) against the Orco agonist VUAA1 (>98 % purity, Sigma-Aldrich), which directly activates the Orco protein in nearly all insect species tested to date43,48,65 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... retina and choroid tissues dissected out from the L-CNV and untouched control mice (four eyes) were lysed for 20 min on ice in radioimmunoprecipitation assay (RIPA) buffer (Sigma #R0278) with protease inhibitors (cOmplete mini ...
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bioRxiv - Molecular Biology 2022Quote: Cell proliferation assays were performed with insect cell lines (Aag2 [A. aegypti], AsE01 [A. suspensa], and S2 [D. melanogaster]) using the WST-1 assay (Sigma-Aldrich). Cells (Aag2 ...
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bioRxiv - Developmental Biology 2019Quote: ... and homogenized in ATP assay Lysis buffer (6 M Guanidine-HCl, 100 mM Tris pH 8 and 4 mM EDTA, all from Sigma Aldrich, Cat# G3272 ...
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bioRxiv - Cancer Biology 2019Quote: ... digital PCR was carried out using a Biomark HD (Fluidigm) with a 65bp TaqMan assay for the housekeeping gene RPP30 (Sigma Aldrich) (7) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The expression level of each enzyme was quantified using radioactive leucine incorporation assays as previously described.(Jewett et al., 2008) All reagents and chemicals were purchased from Sigma-Aldrich unless otherwise specified.
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase and Renilla activity were determined in a Tecan plate luminometer with freshly reconstituted assay reagents (0.5 mM D-Luciferin (Sigma-Aldrich, L9504), 20 mM tricine ...
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bioRxiv - Pathology 2020Quote: ... The amount of attached cells (from the washed plate) and the total cells (from the unwashed plate) was determined using XTT assay (Sigma, # 11465015001) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: Growth versus non-growth phenotypes in bulk liquid culture were assessed using a cell proliferation assay with the non-toxic green fluorescent membrane linker dye PKH67 (Sigma-Aldrich). To ensure that Methylobacterium cells could be easily distinguished from background events in flow cytometry ...
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bioRxiv - Genetics 2020Quote: ... and MILLIPLEX MAP Mouse Cardiovascular Disease (CVD) Magnetic Bead Panel 1-Cardiovascular Disease Multiplex Assay (#MCVD1MAG-77K-02, all from Millipore Sigma) were used to analyze cytokines and chemokines from mouse plasma ...
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bioRxiv - Cell Biology 2020Quote: ... Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution; SIGMA; cat T8154).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Larvae were collected and prepared for the assay as detailed above except that they were kept in food with 40 μM all trans-retinal (ATR, Sigma-Aldrich) and in dark for 72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The biochemical assay of GCase activity was carried out in a buffer containing 4-methylumbelliferyl beta-d-glucopyranoside substrate (Cat. M3633, Sigma Aldrich); after 1 hour at 37°C the reaction was stopped with 0.25 M glycine buffer pH 10.4 and the fluorescence emission of the 4-methylumbelliferyl generated (4-MU ...
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bioRxiv - Plant Biology 2020Quote: Spin trapping assays with the spin probe 4-pyridyl-1-oxide-N-tert-butylnitrone (4-POBN; Sigma-Aldrich, St. Louis, USA) to detect the formation of hydroxyl radicals24 were carried out using cyanobacterial cells (OD730 = 0.65 ...
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bioRxiv - Cell Biology 2019Quote: ... treated (5 Gy) fixed (1h post IR) with formaldehyde (3.7% in PBS) and the PLA assays (Duolink PLA technology, Sigma-Aldrich, DUO92014) performed using manufacturer’s protocol with antibody staining performed as in the standard immunofluorescence procedure with antibodies against V5 tag and Treacle or NBS1 and Treacle
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bioRxiv - Microbiology 2021Quote: ... permeabilized with 0.2% Triton X-100 (in Phosphate Buffered Saline [PBS]) and subjected to an immunofluorescence assay (IFA) using a monoclonal antibody (MAb) anti-ACE2 (Sigma-Aldrich), and then incubated with a goat anti-rabbit IgG (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2020Quote: Alkaline phosphatase activity (APA) was evaluated through fluorometric assay following the hydrolysis of the substrate (S) 4-methylumbelliferyl phosphate (MUF-P, Sigma-Aldrich) to 4-methylumbelliferyl (MUF) ...
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bioRxiv - Microbiology 2020Quote: ... For the phenotypic and proteomic assays after treatment with hydrogen peroxide a final concentration of 5 mM and 10 mM were selected (Sigma-Aldrich). For susceptibility assays C ...
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bioRxiv - Immunology 2021Quote: ... obtained from the NA activity assay of the PV to a standard curve of 4-Methylumbelliferone sodium salt 4-MU(SS) (Sigma M1508). NA activity is based on the production of 4-MU over time (60 minutes at 37 °C for the standard assay) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged and protein concentration measured using a bovine serum albumin standard curve and a bicinchoninic acid (BCA) assay (Sigma-Aldrich). The WES capillary electrophoresis system (ProteinSimple ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells grown in hypoxic chambers were frozen at the time points mentioned in the results section and harvested all together by lysing in Radioimmunoprecipitation assay buffer (RIPA buffer) containing 1× protease inhibitor cocktail (Sigma-Aldrich). For each sample ...
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bioRxiv - Immunology 2022Quote: ... Cell number and viability were examined each day in triplicate wells using trypan blue exclusion and cell proliferative assay (# Cat. no. T8154, Sigma-Aldrich). The assay was performed for 10 d post-seeding i.e. ...
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bioRxiv - Cell Biology 2022Quote: ... pulverized in liquid nitrogen and protein was extracted using a 1x radioimmunoprecipitation assay buffer (50mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Nonidet®40 Substitute (74385; Sigma-Aldrich), 0.5% sodium deoxycholate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified using a chloramine-T assay (Huszar et al., 1980) with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Cell Biology 2022Quote: ... The following small molecules required for the MTS assay were ordered individually and 10 mM stocks prepared and stored at −20°C: (CCCP, Sigma, C2920), (Oligomycin ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Microbiology 2022Quote: ... The LDH assay was carried out in 96 or 384 well plate format and a recombinant porcine LDH enzyme (Sigma-Aldrich) was used in each LDH assay plate in a serial dilution to extrapolate a standard curve which was used to estimate LDH released on infection with A ...
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bioRxiv - Physiology 2022Quote: ... and 120 minutes following the injection using a colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO). Area under the curve measurements were calculated using the trapezoidal method (37).
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bioRxiv - Plant Biology 2022Quote: ... Quantitative measurements were performed with an enzymatic assay using myrosinase/thioglucosidase from Sinapis alba (Sigma-Aldrich CAS-No. 9025-38-1) and a D-Glucose Assay Kit (glucose oxidase/peroxidase ...
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bioRxiv - Physiology 2022Quote: ... Samples were then incubated for 15 min at room temperature with assay buffer containing glucose oxidase (0.2 U/mL, Sigma-Aldrich G7141), horseradish peroxidase (0.3 U/mL ...
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bioRxiv - Neuroscience 2022Quote: ... Concentrations of RMAs in the collected blood were determined using the standard curves generated by the luciferase assays conducted on the normal mouse serum (Sigma-Aldrich) added with RMAs at known concentrations ...
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bioRxiv - Bioengineering 2022Quote: ... Hydroxyproline content as a measure for collagen was quantified in scaffolds using a chloramine-T assay [35] with trans-4-hydroxyproline (H54409, Sigma-Aldrich) as reference ...
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bioRxiv - Bioengineering 2022Quote: ... content of scaffolds was measured using a dimethyl methylene blue (DMMB) assay [36] with shark cartilage chondroitin sulfate (C4284, Sigma-Aldrich) as a reference ...
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bioRxiv - Cell Biology 2022Quote: ... and homogenized using a Potter-Elvehjem tissue grinder on ice in ice-cold incomplete radioimmunoprecipitation assay buffer (RIPA) containing 50 mM Tris-HCl pH 7.4 (Sigma-Aldrich, T1503) and 100 mM NaCl (Millipore ...