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Citations for Millipore Sigma :
2401 - 2450 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 10 μl of cell lysate was transferred to a 96-well clear assay plate and made up to 100 ul with 90 μl phosphatase substrate (Sigma) in 1.5 M alkaline buffer solution (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... No HCYTOMAG-60K) were quantified using Milliplex MAP Luminex microbead assays according to the manufacturer’s instructions (Merck Millipore, Darmstadt, DE). Samples were analyzed without dilution in duplicate ...
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bioRxiv - Immunology 2023Quote: Chemotaxis assays were carried out using 24-well Transwell chambers with 5-μm pore size polycarbonate membranes (Sigma, #CLS3421-48EA). B cells (107 cells/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected after centrifuging at 4°C for 10 minutes and the protein amounts were determined by BCA assay (Sigma). The proteins were denatured by boiling for 10 minutes in the DTT containing sample buffer and separated by 4-20% Mini-PROTEIN TGX gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then incubated on a shaker for 10 minutes in radio-immunoprecipitation assay (RIPA) buffer (#R0278, Sigma, UK) and 1% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... The ASA content of tomato fruits was determined using a titration assay and standard curve with commercial ascorbic acid (S88476, Sigma) and 2,6-dichloroindophenol (119814 ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability in 2D monolayer cultures ± treatment was quantified using an XTT assay according to the manufacturer’s protocol (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... and the decanted supernatant was quantified with a microplate Bradford assay per manufacturer’s instructions (Sigma-Aldrich, Sant Louis, MO, USA).
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bioRxiv - Biochemistry 2023Quote: ... 50 µM sphinganine (2.5 µL 500 µM sphinganine in Assay Buffer containing 10% ethanol) and 50 µM palmitoyl-CoA (Sigma-Aldrich) (2.5 µL 500 µM palmitoyl-CoA in Assay Buffer ...
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bioRxiv - Bioengineering 2023Quote: The viability of the encapsulated islets was assessed after 24 days of incubation using two different methods: the resazurin assay for quantification (Sigma) and the Live/Dead assay for visualization (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... the substrate stocks were diluted with assay buffer (50 mM Tris-HCl, pH 8.0) additionally containing 1 mg ml-1 gum arabicum (Sigma Aldrich). All values were determined in quadruplicates and corrected for autohydrolysis using the experimentally determined pH-dependent extinction coefficient of p-nitrophenol in microtiter plates (ε = 9.3024 mL µmol-1) ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then washed twice with PBS and processed using Duolink Proximity Ligation Assay (PLA) reagents (In-Situ PLA Probe Anti-Mouse MINUS, Sigma DUO92004; In-Situ PLA Probe Anti-Rabbit PLUS, Sigma DUO92002 ...
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bioRxiv - Cancer Biology 2024Quote: ... The tubes were centrifuged at 6,000 rpm and the supernatants were collected for cytokine analysis by bead-based multiplex assay (MILLIPLEX MAP Mouse High Sensitivity T Cell Panel, Millipore) following the manufacturer protocol27.
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration in the remaining supernatant was assessed using a Bradford reagent assay according to manufacturer’s protocol (Sigma-Aldrich - B6916), and normalized on collected fecal weight and on animal weight.
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bioRxiv - Cancer Biology 2024Quote: ... Australia) and the viability was assessed by the resazurin-based Alamar Blue assay (#R7017, Sigma-Aldrich, St. Louis, MO, USA) and the florescence intensity in each well was measured after 90 mins using a bottom-reading florescent plate reader (CLARIOstar Plus ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to 1 mg/ml and processed by Eve Technologies using the Mouse High Sensitive 18-Plex Discovery Assay according to the manufacturer’s protocol (Millipore Sigma).
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bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
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bioRxiv - Microbiology 2024Quote: ... the virus titre was determined by plaque assay on MDCK cells as described previously using a final concentration of 1 µg/ml of TPCK trypsin (SIGMA)[22].
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bioRxiv - Neuroscience 2024Quote: Four-day old adults were randomly collected during paralysis assays and stained with 1 mM X-34 (Sigma, Cat # SML1954) 10 mM Tris-HCl pH 8.0 for 2 hours as previously described (Link et al. ...
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bioRxiv - Bioengineering 2024Quote: ... and polyIC incubation was evaluated using the MTT (3-(4, 5dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). The cells were seeded into 96 well-format cell culture plates at a seeding density of 8ξ103 cells per well ...
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bioRxiv - Cell Biology 2024Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in pre-warmed XF assay media to achieve the following final concentrations in the cell culture well: 1.5 μM Oligomycin A (Sigma, 75351); 1 or 0.5 μM FCCP (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed using a commercial kit (In Situ Cell Death Detection Kit, TMR (Sigma, 12156792910)) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2023Quote: ... osteoclasts were evaluated by TRAP staining using a commercially available kit (Acid Phosphatase TRAP kit, Sigma–Aldrich), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... primary antibodies were removed with PBS and cells were subjected to the Duolink™ Proximity Ligation Assay protocol according to manufacturer instructions (Sigma: DUO92008 ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using a commercial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) viability assay (Sigma Aldrich, Rehovot, Israel). That assay was used to determine how highly 4T1 proliferation was stimulated by Norepinephrine (NE ...
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bioRxiv - Genetics 2021Quote: ... Telomere length was measured by two orthogonal methods: 1) qPCR8 and 2) telomere restriction fragment (TRF) analysis using TeloTAGGG Telomere Length Assay (Sigma Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2022Quote: All enzyme activity were determined by measuring the release of phosphate and formation of a green complex formed between malachite green oxalate with phosphomolybdate and using the malachite green assay as previously described (55) with slight modifications and manufacturer specifications (Sigma-Aldrich). 40 μL of M87SpastinA2C and VPS4BS2C in ATPase buffer (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... ATP-hydrolysis rates were measured over the first ~2 min by monitoring the loss of absorbance at 340 nm using a coupled NADH–ATP assay (Burton et al., 2001) with 5 mM ATP (Sigma-Aldrich), pyruvate kinase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2019Quote: ... A cell free assay was prepared in which 650 somite strips were homogenized in HENS buffer [1ml + 10μl protease inhibitor cocktail (Sigma Lot# 033M4023V)] using an electrically-driven disposable pestle and grinding resin (GE Healthcare) ...
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bioRxiv - Cancer Biology 2020Quote: The sections from two biological replicate experiments were stained using a Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay (Millipore S7165), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... A Bradford colorimetric assay was used to normalize protein concentrations and 35ug lysate in 25uL volume was analyzed in the Luminex Multiplex Assay (EMD Millipore) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation, Billerica, MA, USA) was used to measure IgG1 ...
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bioRxiv - Microbiology 2021Quote: Guanosine nucleotide hydrolysis was assessed through synthetase assays performed as above using guanosine 5’-β-thio-diphosphate trilithuim salt (GDPβS) as the only nucleotide phosphoacceptor (Millipore Sigma). The reactions were caried out for 60 mins at 37°C and the spots were autoradiographed as reported above.
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bioRxiv - Microbiology 2021Quote: ... moscoviensis was assessed by measuring total cell protein with the enhanced test tube protocol of the BCA protein assay (Sigma-Aldrich).
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was assayed for glucose concentration using glucose oxidase/peroxidase colorimetric glucose assay with 45 μg/ml o-dianisdine dihydrochloride (Sigma-Aldrich), and absorbance measured at 450 nm ...
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bioRxiv - Biochemistry 2021Quote: Growth for the heme trafficking dynamics assay was accomplished by culturing HS1-expressing cells with or without 500 μM SA (Sigma-Aldrich) in SC media lacking leucine ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed for 30 minutes on ice in Radioimmunoprecipitation assay (RIPA) buffer (Sigma Aldrich, St. Louis, MO, USA, #R0278) supplemented with cOm-plete™ EDTA-free Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The concentration of inorganic phosphate released by hydrolysis of ATP was measured by tracking absorbance at 620 nm of malachite green binding to phosphate ions using the ATPase/GTPase activity colorimetric assay (Sigma-Aldrich) as described (14).
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bioRxiv - Cell Biology 2021Quote: Transferrin uptake assays were performed by serum-starving cells in DMEM/f12 with 0.5% (w/v) BSA (Sigma Life Sciences A9647) for 30 minutes followed by adding fresh DMEM/f12/ BSA with alexa-fluor 647-labeled transferrin at a concentration of 10 micrograms/milliliter (Jackson Immunoresesarch 009-600-050) ...
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bioRxiv - Cell Biology 2021Quote: ... were equilibrated in 500 μl of assay buffer (50 mM Tris-HCl, pH 8, 150 mM NaCl, 1% Triton X-100, PhosSTOP (Millipore-Sigma), and cOmplete Protease Inhibitor Cocktail tablet ...
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bioRxiv - Microbiology 2020Quote: ... J774A.1 cells were maintained in DMEM supplemented with heat inactivated serum and the assay was complemented with 1 μg/mL C3 (Sigma, C2910) or C3b (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cell lysates were prepared using Radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with protease inhibitors (cOmplete Protease inhibitor cocktail, Sigma-Aldrich, 11697498001) and 50mM 1,4-Dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were homogenized in 10 volumes of ice-cold radioimmunoprecipitation assay buffer containing protease inhibitor cocktail (Sigma-Aldrich, St. Louis, Missouri), phosphatase inhibitor cocktail (PhosStop ...
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bioRxiv - Neuroscience 2019Quote: ... Equal amounts of protein (measured by BCA assay-Pierce) were used for free heme measurement following the manufacturer’s instructions (Sigma MAK-036).
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bioRxiv - Physiology 2020Quote: ... Samples were then incubated for 10 min at room temperature with assay buffer including glucose oxidase (0.25 U/mL, Sigma-Aldrich, G7141) and horseradish peroxidase (0.17 U/mL ...