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Citations for Millipore Sigma :
2651 - 2700 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... containing 12.5µL of assay buffer (125mM KCl, 20mM HEPES, 2mM MgCl2, 2mM KH2PO4, pH =7.4) supplemented with 40mM succinate (Sigma-Aldrich # S3674-100G), 40mM malate (Sigma-Aldrich #240176-50G) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then treated in triplicates for 24hrs with varying concentrations of rhIL-23 for analysis via a WST-1 proliferation assay using WST-1 reagent (0501594400, Sigma-Aldrich, Canada) and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091 ...
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bioRxiv - Molecular Biology 2024Quote: ... were transferred to a 96-well plate and overlayed with 120 µL of assay buffer containing DTNB (D8130, Sigma; 2 mg/ 3mL) and 10 U glutathione reductase (G3664 ...
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bioRxiv - Physiology 2024Quote: ... Assay-specific metabolic compounds were diluted day-of in XF DMEM media immediately prior to experiments: Oligomycin 5μM (Sigma Aldrich cat. 495455), Rotenone 2.5μM (Sigma Aldrich cat ...
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bioRxiv - Systems Biology 2024Quote: ... or thawed on ice from frozen preparations and 25 μg from each sample was prepared for glutamate dehydrogenase assay according to manufacturer instructions (Millipore Sigma MAK099). The absorbance at 450 nm was then measured every two minutes for 4 hours on Cytation 5 (Biotek ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cancer Biology 2022Quote: ... EV were labeled with a PKH67 dye labeling kit-green (or PKH26 dye labeling kit-red) following the manufacturer’s instructions (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2020Quote: 15N-accumulation assay after 15N-nitrate or 15N-ammonium labeling was performed with 15N-labeled KNO3 (99 atom % 15N, Sigma-Aldrich, no. 335134) or 15N-labeled NH4Cl (98 atom % 15N ...
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bioRxiv - Microbiology 2021Quote: ... Alginate lyase was removed from culture medium prior to assay by filtering the medium through 3 kDa Amicon Ultra molecular weight cutoff filters (MilliPore Sigma, Burlington, MA). To maintain plasmids in 12B01,12.5 µg/mL chloramphenicol was added to the medium.
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Components for solubilisation buffer and ligands for both ThermoFRET stability assay and fluorescent ligand binding experiments were obtained from Sigma-Aldrich (Havervill, UK) and Anatrace (Ohio ...
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bioRxiv - Biophysics 2019Quote: ... Purity was examined by SDS-PAGE and concentration determined by Bradford assay before loading onto a 20 mL spin column packed with 5 mL ssDNA-cellulose (Sigma Aldrich D8273-5G). The column with SSB containing fractions was sealed and incubated for 60 min with gentle rocking ...
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bioRxiv - Biochemistry 2020Quote: ... The GnT I assay was subsequently performed in GnT I activity buffer [63] containing 5 mM of UDP-GlcNAc (Sigma-Aldrich Chemie GmbH).
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Microbiology 2020Quote: The MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex – Immunology Multiplex Assay (Millipore, Burlington, MA, USA; #PCYTMG-40K-PX23) was performed on collected plasma samples following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... A portion of the frozen somatosensory cortex\tissue was homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease and phosphatase inhibitors (SigmaFast, S8820, Sigma-Aldrich, USA and PhosStop, 4906837001, Sigma-Aldrich, USA) The protein concentration of the lysates was quantified using a BCA Protein Assay Kit (Thermo Scientific Pierce Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Samples obtained from in vitro proteolysis assay were analyzed by electrophoresis on an 8% SDS-polyacrylamide gel followed by transfer to Immobilon polyvinylidene difluoride membranes (Millipore Co., Bedford, MA). The membranes were blocked using 4% non-fat milk and immunostained with specific mAb ...
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bioRxiv - Cancer Biology 2020Quote: ... or only on the bottom side (for tumor haptotaxis assays) of 8 or 3 μm hanging cell culture inserts (Millipore, MCEP24H48 and MCSP24H48). After washing the filters with PBS ...
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bioRxiv - Pathology 2021Quote: ... ALT was measured in 1 microliter of plasma collected via cardiac puncture or tail vein bleed using a colorimetric assay (Millipore Sigma cat. # MAK052) according to the manufacturer’s instructions.
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Neutralization assays were performed by pre-incubating cells with anti-GalactosylCeramide antibody (mGalC, 10µg/mL, Millipore 10µg/mL, 30 min at 4°C) or viral inoculum with monoclonal antibody against HIV-1 gp120 (1-2µg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 24h before monitoring cell viability using the MTT assay: cells were incubated at 35°C for 1 h with MTT (Sigma, Cat #M2128-10G). For that purpose ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of siderophores produced by AMB-1 was performed using the Chrome Azurol S (CAS) assay (199532, Sigma-Aldrich, St. Louis, Missouri, USA) [47] ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the workflow of this assay involves four main steps: (i) Tissue is harvested and lysed in lysis buffer containing S-Methyl Methanethiosulfonate (MMTS, Sigma, Cat no. 64306) to block free thiol groups (-SH) ...
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bioRxiv - Physiology 2022Quote: Tissue triglyceride levels were measured using an enzymatic triglyceride assay (Glycerol Reagent, Cat# F6428, Triglyceride Reagent, Cat#T2449, Sigma-Aldrich, Oakville, ON, Canada). In a flat bottom 96 well plate ...
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bioRxiv - Physiology 2024Quote: ... [NH3 + NH +]) was determined spectrophotometrically in 25 µL aliquots of flash frozen plasma by enzymatic ammonia assay (Sigma-Aldrich, USA, Catalog number: AA0100). [TAmm] was calculated from the delta absorbance at 340 nm wavelength before and after the addition of the enzyme L-glutamate dehydrogenase ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Microbiology 2023Quote: Proteins were isolated from hPCLS with the radioimmunoprecipitation assay (RIPA) buffer (Thermo, Cat.# 89901) containing a cocktail of protease inhibitors (Sigma, Cat.# P8340-5ML) and phosphatase inhibitors (Fisher ...
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bioRxiv - Genetics 2023Quote: ... exact number recorded per vial for assay read-out) were then exposed to standard medium prepared with 200mM Mifepristone (RU486) from Sigma Aldrich (cat# 856177) and 2.5% w/v FD&C Blue Dye no 1 from Spectrum (cat# FD110 ...
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bioRxiv - Microbiology 2022Quote: ... The precipitates were extracted into 1x SDS sample buffer containing 2-mercaptethanol and subjected to a standard immunoblotting assay using HRP-conjugated anti-HA antibody (H6533; Sigma; 1:3000 dilution) and anti-FLAG antibody (A8592 ...
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bioRxiv - Microbiology 2022Quote: ... Iron concentrations were then normalized to the protein content in each sample using the bicinchoninic acid (BCA) assay (Sigma Aldrich, St. Louis, MO). Quantification was performed from 3 independent cultures for each strain.
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bioRxiv - Evolutionary Biology 2023Quote: ... three times with a sharp metal rod of the same diameter as the calibrated glass micropipettes used for the assays (5 μL, Sigma, product number P0549). Three glass micropipettes per vial were filled with liquid media ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the plasmid in floral tissue of T2 plants was confirmed by GUS staining assay following the manufacturer’s protocol (Millipore Sigma, Burlington, MA, USA) (Figure S6) ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Microbiology 2023Quote: Protein lysates from luciferase assays were subjected to 8% SDS-PAGE gel and transferred to PVDF membranes (Millipore-Sigma, St. Louis, MO, USA). Membranes were incubated with 5% nonfat milk at room temperature for 15 minutes for blocking ...
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bioRxiv - Cancer Biology 2023Quote: Proximity ligation assays (PLA) were performed according to manufacturer’s protocol for both flow cytometry and in situ confocal microscopy detection (Sigma, DUO94104 and DUO92013 respectively). For flow cytometry ...
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bioRxiv - Physiology 2024Quote: ... Concentrations of plasma TG in the arterial and venous samples were determined enzymatically using a commercially available assay (Sigma-Aldrich, St. Louis, MO) and by calculating an average concentration based on ten replicates.
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bioRxiv - Neuroscience 2024Quote: ... Cells were counted and seeded (400,000 cells/mL for survival assays) on poly-L-lysine treated glasses (MW 350 kDa, Sigma Chemical, St. Louis, MO). The feeding medium was replaced to growth media ...
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bioRxiv - Bioengineering 2024Quote: ... Metabolic and mitochondrial activity of host cells was assessed with a resazurin assay (7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt, Sigma-Aldrich, Overijse, Belgium). Medium was refreshed with 1 mL of 0.01 mg/mL resazurin in DMEM and incubated for 2 h at 37°C ...
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bioRxiv - Genetics 2024Quote: Snap frozen gastrocnemius or spinalis muscles were homogenised in 4% SDS lysis buffer and assessed for total protein concentration using Direct Detect Assay free cards (Millipore, Cat #DDAC00010-GR). Proteins were separated by SDS-PAGE using stain-free precast midi- criterion gels (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich). Inserts were sequenced (GATC Services ...
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bioRxiv - Cancer Biology 2021Quote: Cell Counting Kit-8 (CCK-8; Sigma Aldrich) was used to measure cell viability according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... ApopTag Peroxidase In Situ Apoptosis Detection kit (Millipore) was used to detect apoptotic cells in whole mount embryos ...
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bioRxiv - Physiology 2022Quote: EdU Cell Proliferation Kit (Sigma-Aldrich; Buchs, Switzerland) was used according to the manufacturer’s instructions ...
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A robust method for generating, quantifying and testing large amounts of Escherichia coli persistersbioRxiv - Molecular Biology 2020Quote: Diluent C (Sigma, included in the PKH67 kit). Store at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: Nuclei EZ Prep Nuclei Isolation Kit from Sigma was used to isolate nuclei from GM12878 cells.
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bioRxiv - Cancer Biology 2019Quote: ... 63 labelled with Nick Translation Kit (Sigma-Aldrich). Image Lab™ Software (Biorad ...