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Citations for Millipore Sigma :
2351 - 2400 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... The clean pellet was resuspended in 0.5–1 ml MAITE+BSA-FFA buffer and the concentration was quantified using the Bradford assay (Sigma-Aldrich). Mitochondria were stored at -80°C in MAITE+BSA-FFA buffer or centrifuged for 10 min at 10000 × g and 4°C and subsequently resuspended in the buffer required for the following analysis ...
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bioRxiv - Physiology 2019Quote: ... the lead acetate/lead sulfide assay is setup by initially preparing the reaction mixture of 10mM L-cysteine (Sigma #168149) and 1mM pyridoxal phosphate (PLP ...
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bioRxiv - Plant Biology 2019Quote: ... Then 25 uL of a dilution 1:10 were used to determine inorganic phosphate concentrations using the molybdate phosphate assay (Sigma), following kit instructions ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... Real-time RT-PCR (TaqMan) assay-specific primers and probes for ZIKV were designed by Sigma-Aldrich (St Louis, MO) following the protocol developed by the Molecular Diagnostics and Research Laboratory Centers for Disease Control and Prevention (CDC) ...
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bioRxiv - Cell Biology 2019Quote: The viability of cells was determined using the standard MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (MTT, Sigma-Aldrich, PL). The cells were incubated on 96-well plate (Perkin Merkel ...
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bioRxiv - Microbiology 2021Quote: ... Sensitivity to micafungin was measured at least in triplicate in a polymerization assay using a 96-well multiscreen high-throughput screen filtration system (Millipore) with a final volume of 100 µl ...
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bioRxiv - Physiology 2021Quote: ... an isotonic solution containing luciferase-luciferin (LL) was prepared from a vial of ATP assay mix-lyophilized powder (Sigma-Aldrich) was used ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Female mice were primed subcutaneously 48 hours before the assay with 0,1ml estrogen (1mg/ml, Sigma E815 in sesame oil) and 4 hours before the assay with 0,1ml progesterone (5mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... The protein concentration of cell lysates was measured using Bradford assay and equal amount of protein was added to anti-flag M2 magnetic beads (Sigma) in the presence of RNaseA (50 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Cytokines and chemokines in the homogenates were quantified using Luminex technology with a MILLIPLEX Map Mouse Cytokine/Chemokine Magnetic Bead Panel 25-plex Immunology Multiplex Assay (Cat. No. MCYTOMAG-70K-PMX, Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged at 10,000 g for 5 minutes and total protein in the supernatant was measured using the bicinchoninic acid (BCA) assay (Sigma). Primary antibodies were detected using a secondary antibody conjugated to horseradish peroxidase ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell viability in response to drugs in 2D monolayer cultures was quantified using an XTT assay according to the manufacturer’s protocol (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Growth assay for inulin as carbon source was carried out by adding 20 µl of 20% inulin (Inulin from chicory, Sigma) or 20% D-Glucose as positive control into 1 ml minimal medium ...
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bioRxiv - Immunology 2021Quote: ... Transfected cells were then washed with phosphate-buffered saline (PBS) and lysed with radioimmunoprecipitation assay buffer (RIPA buffer) (Sigma, Germany). The harvested cell lysates were subjected to western blot analysis to verify the expression of S protein using in house mouse anti-S (SARS-CoV-2 ...
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bioRxiv - Immunology 2020Quote: For the proliferation assay splenocytes were plated in a Corning Costar 96-well round bottom cell culture plate (Sigma-Aldrich) at a cell density of 2.5 × 105 cells in a final volume of 170 µL ...
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bioRxiv - Immunology 2021Quote: Cytotoxicity of the isolated PAC used for the stimulation of murine macrophages was assessed using neutral red assays (Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: The following primary antibodies were used for immunoprecipitation assay and/or immunoblotting: a mouse anti-Flag and a mouse anti-Flag-HRP antibody (Sigma), a rabbit anti-N antiserum (70) ...
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bioRxiv - Cell Biology 2021Quote: ... Extraction of RNA from bead sprout assays (described below) using TRIzol was performed after the removal of HDFs with trypsin-EDTA Solution 10X (59418C, Sigma). cDNA was synthesized with iScript Reverse Transcription Supermix (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration for the soluble fraction was adjusted (3µg/ml) following bicinchoninic acid assay measurement (BCA, Sigma-Aldrich, Poole, UK) and dilution in lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: In vitro histone acetylation assays were performed on 25 μg of mitotic chromatin (isolated as described above and quantified using the bicinchoninic acid colorimetric assay system (Sigma)) in 50 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... HAT activity was quantified by: i) a colorimetric assay quantifying the release of Co-A using DTNB (2-nitrobenzoic acid) (Sigma) by measuring the absorbance at 412nm ...
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bioRxiv - Bioengineering 2022Quote: MSC derived small EVs were characterized for protein concentration using the bicinchoninic acid (BCA) assay (Sigma Aldrich, Oakville, ON, Canada). Nanoparticle tracking analysis was performed using ZetaView (Particle Metrix ...
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bioRxiv - Microbiology 2022Quote: Total protein was extracted from LT toxin-treated (100ng/ml) human differentiated enteroid monolayers (235D line) and untreated control using radioimmunoprecipitation assay (RIPA) buffer (Sigma) containing protease inhibitor cocktail ...
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bioRxiv - Plant Biology 2022Quote: ... the water was removed and replaced with ROS assay solution (100 μM Luminol [Millipore-Sigma], 20 μg/mL horseradish peroxidase [Millipore-Sigma] with or without immune elicitors) ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Synthetic Biology 2023Quote: HEK293T-LP cells sourced from Matreyek et al38 were plated into a 48 well plate according to the plate reader assay and treated with 0 to 2 ng/μl dox (Sigma) to induce BFP expression ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: Enzymatic Assays from skeletal muscle tissues were done from muscle lysates prepared in the assay buffer as per the manufacturer’s protocol (Sigma-Aldrich). Serum calcium levels were measured using calcium estimation kit (Abcam #ab102505 ...
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bioRxiv - Neuroscience 2022Quote: The cellular viability of the brain-like tissues was tested using a lactate dehydrogenase assay (LDH) (Sigma, cat.no. MAK066-1KT) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: The etiolated seeding triple response assay was performed with sterilized seeds on various concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC, Sigma) plates (0 ...
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bioRxiv - Plant Biology 2024Quote: Ethylene triple response assay was performed with sterilized seeds on MS plates containing various concentrations of 1-aminocyclopropane-1-carboxylic acid (ACC, Sigma) (0 ...
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bioRxiv - Neuroscience 2024Quote: ... Sigma-Aldrich)-coated 6-well plates (200,000 cells/well) for western blot experiments and in 96-well plates (10,000 cells/well) for MTT assay (#M2003, Sigma-Aldrich).
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bioRxiv - Physiology 2024Quote: ... Triglyceride concentrations were determined using a spectrophotometric assay with a 4:1 Free Glycerol Agent / Triglyceride Agent solution (Cat#: F6428 & T2449, Sigma, Free-Glycerol reagents and Triglyceride ...
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bioRxiv - Neuroscience 2024Quote: Detection of newly synthesized proteins by proximity ligation assay was carried out using anti-puromycin antibody in combination with protein-specific antibodies and detection using Duolink reagents (Sigma) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
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bioRxiv - Immunology 2024Quote: WB analysis of protein levels was performed on whole-cell lysates prepared by lysis in radioimmunoprecipitation assay buffer (Sigma-Aldrich) supplemented with Benzonase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: gGT-activity was determined by a colorimetric assay based on the release of p-nitroaniline from the substrate gGpNA (Sigma). The method was adapted from Meister et al ...
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bioRxiv - Immunology 2024Quote: ... After 24hrs supernatant was collected and the cytokine expression was analyzed by luminex bead array assay (Millipore Sigma, Burlington, MA). In separate cultures ...
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bioRxiv - Immunology 2024Quote: ... Each 1 ml collected fraction was analyzed for protein concentration using a Nanodrop spectrophotometer (absorbance at 280 nm) and pooled three fractions were further analyzed for protein concentration using Bradford assay (Sigma) and used to measure biological activity to induce Arg1 expression in RAW264.7 macrophage cell line ...
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bioRxiv - Biochemistry 2023Quote: ... A 12.5 µM solution of fluorescently labeled tubulin in 1x assay buffer containing an oxygen scavenger system (freshly mixed 0.035 mg/ml catalase (Sigma Aldrich), 0.2 mg/ml glucose oxidase (Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... The final experimental mix was prepared on ice: assay buffer supplemented with oxygen scavengers (0.1 mg mL-1 catalase (Sigma-Aldrich), 1 mg mL-1 glucose oxidase (Serva)) ...
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bioRxiv - Immunology 2023Quote: ... IL-1Rα and IL-10) were analyzed by multiplex assays using custom multiplex cytokine plates (EMD Millipore, Billerica, MA, USA). Data were collected on a MAGPIX instrument (Luminex ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The agarose affinity gels were washed with PBS five times and subjected to SDS-PAGE assay and Western blotting using rabbit anti-GFP antibody (Sigma), rabbit anti-V5 antibody (Merck ...
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bioRxiv - Plant Biology 2023Quote: ... adjusted to an OD600 of 0.3 for cell death and electrolyte leakage assays and an OD600 of 0.1 for immunoblot assays (final concentration for each strain in a mixture) and were incubated with 100µM acetosyringone (Sigma-Aldrich) for 2-4 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... All remaining enzyme activity levels were determined using colorimetric and fluorescence based assays purchased from Sigma-Aldrich (St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... primary antibodies were removed with PBS and cells were subjected to the Duolink™ Proximity Ligation Assay protocol according to the manufacturer’s instructions (Sigma: DUO92008 ...
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bioRxiv - Microbiology 2023Quote: ... L (+) lactic acid production in the lung tissue and BAL was also measured using a Lactate Colorimetric Assay (MAK064, Sigma).
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bioRxiv - Microbiology 2023Quote: ... The supernatant was used for FLAG purification in pull-down assays with Anti-Flag® M2 Magnetic Beads (Sigma-Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... and in vitro assays on SK-MEL-28 cells were as follows: 4-Hydroxyanisole (4-HA, Sigma-Aldrich, MO, USA), the canonical Wnt pathway antagonist IWR-1 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: The cells were incubated with the assay buffer (HBSS supplemented with 10 nM HEPES and 4.5 mg/ml glucose) containing 50 µM testosterone (T1500, Sigma-Aldrich) at 37°C for 2 h and the assay medium was withdrawn at 30 ...