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Citations for Millipore Sigma :
2601 - 2650 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Subcellular proteome extraction kit (Cat# 539790, Millipore). Antibodies used are as follows ...
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bioRxiv - Neuroscience 2024Quote: ... using the Duolink Probemaker kit (Sigma-Aldrich). “Indirect” PLAs for nitrated hASYN and nitrated NDUFB8 were performed using probes already conjugation to secondary antibodies and provided in a PLA kit (Duolink ...
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bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
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bioRxiv - Genomics 2020Quote: For THP-1/MV4;11 competitive proliferation assays 2 independent INTS6 sgRNAs or a control Scrambled (SCR) sgRNA (Sigma Aldrich, Key Resources Table) with BsmBI compatible overhangs were ligated into BsmBI (NEB R0739 ...
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bioRxiv - Plant Biology 2019Quote: ... To determine and quantify these amino acids in plant extracts and enzyme assays standard materials were purchased from Sigma-Aldrich (Steinheim, Germany). Other chemicals were obtained in LC/MS grade from Roth (Karlsruhe ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... was measured with a bicinchoninic acid (BCA) protein assay (Smith et al., 1985) using bovine serum albumin (BSA) as a standard (Sigma Chemicals, USA) with the EnVision microplate reader at an absorbance of 570 nm ...
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bioRxiv - Synthetic Biology 2020Quote: ... whilst AL-PHA assay plates were centrifuged 500 g for 10 minutes (MPS 1000 Mini PCR Plate Spinner, Sigma-Aldrich, MO, USA) in order to pellet AL-PHA beads ...
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bioRxiv - Physiology 2019Quote: Islets (n≥4 mice/sex/treatment group) were lysed in radioimmunoprecipitation assay buffer with protease inhibitor cocktail (Sigma, St. Louis, MO, USA). Lysates were subjected to SDS-PAGE and blotted using antibodies as detailed in Supplemental Table 3 ...
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bioRxiv - Biophysics 2021Quote: Wild type and p75NTR KO CGNs were trypsinized and plated at a density of 40 000 (for cell death assay) or 200 000 (for protein collection) cells per coverslip coated with poly-L-lysine (Sigma, Cat: P7280) in a 24-well (Starlab ...
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bioRxiv - Neuroscience 2021Quote: ... and then lysed with 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signalling Technologies, cat. #9806) and 200 mM phenylmethylsulfonyl fluoride (Sigma, cat. #P7626) with 1× protease inhibitor cocktail (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Plant Biology 2020Quote: ... Detection was carried out by chemiluminescence assays after incubation with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (Sigma or Santa Cruz).
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bioRxiv - Microbiology 2020Quote: ... Simultaneous determination of nitrate and nitrite concentrations induced by the bacteria was done by Vanadium assay with the reduction of nitrate to nitrites by Vanadium (III) combined with detection by the acidic Griess reaction (Sigma-Aldrich, USA), as reported in Miranda et al. ...
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bioRxiv - Microbiology 2021Quote: Cell migration and invasion assays were performed using 24-well transwell chambers with filter membranes 12 µm pore size (Millipore Corporation, PIXP01250). Cells were detached with trypsin-EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse anti-Flag (1:1,000 for western blotting and 2 μg for co-IP assays; F1804; Sigma, St. Louis, MO, USA). Primers and sequence information are provided in Supplementary Table S1.
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bioRxiv - Microbiology 2020Quote: The fluorescent substrate MUC was applied for the Cts1 liquid assay (4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside hydrate; M5639 Sigma-Aldrich) (Koepke et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Serum was collected from groups of mice and an in–house assay was used in which anti–human IgG Fc (Sigma, Catalog # FAB3700259) was used to capture human IgG (MS-Hu6) ...
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bioRxiv - Biochemistry 2020Quote: ... cell lysates were diluted 10-fold and incubated with substrate (5 mM α-naphthyl phosphate, Sigma-Aldrich N7255, dissolved in assay buffer) at 30°C for 20 min ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cell Biology 2022Quote: To evaluate the angiogenic potential of BEC CM a tube formation assay was performed using the HCMEC/D3 line (SCC066 Merck Millipore, Darmstadt, Germany), as described above ...
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bioRxiv - Microbiology 2019Quote: ... TEER was evaluated with the Millicell ERS-2 Voltohmmeter at the beginning and at the end of the assay (Merck-Millipore, Overijse, Belgium).
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bioRxiv - Physiology 2020Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Coating solution was then removed by flicking and plates were washed with OptiMEM and blocked with 25 ul/well of assay buffer (1% Ovalbumin (Sigma #A5503-10G) in OptiMEM containing 1X Pen-Strep (Gibco #15140-148)) ...
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bioRxiv - Physiology 2021Quote: ... and then levels of glucose in both duplicates measured by colorimetric assay following the addition of a glucose oxidase reagent (Sigma; GAGO-20). Levels of glycogen in each experimental sample were then calculated by subtracting the glucose measurements of the untreated duplicate from the amyloglucosidase-treated sample ...
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and urethane (ethyl carbamate, EC; CAS# 51-79-6) from Sigma (St. Louis, MO). Primer sets were Kras ...
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bioRxiv - Microbiology 2020Quote: ... aureus binding to fibronectin and fibrinogen was determined using a semi-quantitative adherence assay on 96-well tissue culture plates (Sigma-Aldrich, USA). Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6 ...
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bioRxiv - Cancer Biology 2022Quote: The spheroid formation assays were carried out on a low attachment U-bottom 96 well plate (BRAND® 96-well microplate, Sigma Aldrich). Single-cell suspension of 500 parental and topotecan resistant Y79 cells in 10% RPMI medium was loaded in each well of a 96 well plate followed by centrifugation for 1000rpm for 1 min to facilitate cell aggregation ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Cancer Biology 2022Quote: Blast colony forming cell (Bl-CFC) assays were performed using iPSC cell pellets resuspended in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma-Aldrich S0192) supplemented with 1% penistreptomycin and L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... IgG3 and IgM) in mice were determined after infection with MORV and VSV using mouse Isotyping multiplex assay (MGAMMAG-300K, Millipore Sigma, USA) by Luminex xMAP technology as recommended by the manufacture.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... magnetic force was applied by placing the assay plates on a handheld magnetic separation block (V&P Scientific 771HH-H/Millipore 40-285). In all subsequent experiments ...
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bioRxiv - Biochemistry 2024Quote: ... Then 20 µl of the mix was transferred to a tube containing 10 µl 3x Stop buffer (assay buffer + 100 mM EDTA, 500 µM proteinase K (Sigma-Aldrich, 107393), 1% SDS ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration in samples was determined using Bradford assay (Bradford, 1976) against standards of known protein concentration prepared using bovine serum albumin (BSA; Sigma-Aldrich, UK).
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bioRxiv - Molecular Biology 2024Quote: The standard assay for ALP activity was carried out in 500 μL of the reaction mixture containing 2 mM pNPP (Sigma-Aldrich, USA) in 0.1 M Tris–HCl buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: ... The proximity Ligation Assay (PLA) was then performed using the Duolink® In Situ Detection Red as recommended by the manufacturer (Sigma Aldrich). After the PLA reaction was performed ...
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bioRxiv - Microbiology 2023Quote: ... Lung homogenates were clarified of debris by centrifugation at 800 xg for 5 minutes then aliquots were frozen at −80°C neat for plaque assay or diluted at a 1 to 4 ratio in TRIzol (Sigma-Aldrich, MA) for RNA isolation and quantitative PCR ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: The Psd enzyme activity assay based on fluorescence was performed with 1,2-diacetyl benzene/β-mercaptoethanol (1,2-DAB/β-ME) (Sigma-Aldrich, Germany), which forms a fluorescent iso-indole-mercaptide conjugate with PE ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Neuroscience 2023Quote: ... the entire frozen neocortex of the left hemisphere was grinded by Dounce homogenizer in RIPA buffer (radioimmunoprecipitation assay buffer, 20-188, Millipore, CA, USA) in the presence of protease inhibitors and phosphatase (Roche ...
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bioRxiv - Genetics 2023Quote: ... The IC90 at day 6 of treatment for each drug was determined by treating the inducible SpCas9 single clone (before gRNA library infection) with a dose curve of each drug in triplicate and taking an MTT assay measurement (Sigma Aldrich 11465007001) on day 6 following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 90 μL of the remaining solution after ALP assay was mixed with 90 μL of digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants containing soluble proteins fraction were transferred in a new Eppendorf tube of 1.5mL and the total protein concentration was determined by BRADFORD assay (Sigma-Aldrich, ref 23238).