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Citations for Millipore Sigma :
2751 - 2800 of 5860 citations for QuantiChrom Hemoglobin Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Using the Spectrum Plant Total RNA kit (Sigma), we isolated RNA from each sample according to the manufacturer’s protocol and performed On-Column DNAse I digestion (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and GenElute™ Bacterial Genomic DNA kit (Sigma), respectively ...
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bioRxiv - Microbiology 2023Quote: ... and the GenElute Bacterial Genomic DNA Kit (Sigma), respectively ...
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bioRxiv - Immunology 2023Quote: ... GenElute Mammalian Total RNA Purification Kit (Sigma-Aldrich) was used to extract the total RNA ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were visualized using ECL kit (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... Trichrome Stain (Masson) Kit (Sigma-Aldrich, Darmstadt, Germany) was used to detect collagens in blue on tissue sections ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...
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bioRxiv - Genetics 2024Quote: ... or GenEluteTM HP Plasmid Midiprep kit (Sigma-Aldrich) or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... which used the DUOlink PLA kit (Sigma Aldrich) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: The bacterial genomic extraction kit (Sigma, Darmstadt, Germany) was used to extract the genomic DNA of MKAK9 ...
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bioRxiv - Cancer Biology 2024Quote: ... or liver dECM hydrogel kit (CC174, Sigma-Aldrich), according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... we obtained all chemicals and kits from Sigma–Aldrich Inc.
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Bioengineering 2020Quote: ... Apoptosis was assessed by DNA fragmentation staining using a commercially available kit (In Situ Cell Death Detection Kit, TMR red, Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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bioRxiv - Neuroscience 2020Quote: TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL ...
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... mRNA was isolated and converted into cDNA using a commercially available kit (GenElute Mammalian Total RNA Miniprep Kit; Sigma-Aldrich, USA). Transcript levels were determined by real-time quantitative polymerase chain reaction (QuantStudio 5 ...
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bioRxiv - Evolutionary Biology 2024Quote: Random-PCR amplification of the Viral DNA or RNA were respectively achieved with the GenomePlex Complete Whole Genome Amplification (WGA) Kit and the TransPlex Whole Transcriptome Amplification (WTA) Kit (Sigma-Aldrich) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... Cell proliferation was determined with the iClick EdU Imaging Kit (ABP Biosciences) and apoptosis was measured using the ApopTag Peroxidase in Situ Apoptosis Detection Kit (Millipore Sigma), following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 100% for 30 minutes each) and then embedded in epoxy embedding medium kit (Epon substitute embedding medium kit; 45359, Sigma Aldrich). For this ...
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bioRxiv - Neuroscience 2021Quote: ... at a density of 40,000 cells/well in 24-well plates for EdU incorporation assays or on 35-mm tissue culture dishes coated with poly-L-lysine (Sigma, P1524; 30 μg/mL) at a density 10×106 cells/dish for immunoblotting ...
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bioRxiv - Plant Biology 2019Quote: ... Reaction products from enzyme assays were analyzed by HPLC using a 125 X 4.6 mm Partisphere SAX-column (Sigma-Aldrich, St. Louis, MO, USA), and a previously described elution gradient (Azevedo et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Proteoglycan content was quantified using the colorimetric dimethyl-methylene blue (DMMB) assay (Sabiston et al., 1985) by measuring the absorbance 525nm with chondroitin sulfate from bovine cartilage as standards (Sigma-Aldrich, St. Louis, MO), and then normalized to wet weight of the IVD ...
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bioRxiv - Physiology 2020Quote: ... Fat tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO). Tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Biophysics 2021Quote: ... The enzyme/protectant mixture was added 1:10 to assay buffer (100 mM Sodium Phosphate, 2 mM Sodium Pyruvate [Sigma-Aldrich], 1 mM NADH [Sigma-Aldrich], pH 6). Enzyme kinetics were measured by NAD+ absorbance at 340 nm ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We 20-fold diluted the MTBD-coated beads in Tris-assay buffer with antifade reagents (125 nM glucose oxidase (G2133, Sigma-Aldrich, St. Louis, MO), 32 nM catalase (C9322 ...
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bioRxiv - Plant Biology 2021Quote: ... The H2O2 concentrations were determined by a standard curve developed using 0.25 – 2.5 μM and normalized by calculating the amount of protein in the extract using a standard Bradford Assay (Bradford, 1976) with Bradford Reagent (Sigma-Aldrich, cat no. B6916).
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bioRxiv - Microbiology 2020Quote: ... Duplicate aliquots (40 μL) of the resulting hemolysates were mixed with 80 μL assay buffer containing 80 μM porphobilinogen (Sigma Aldrich, Castle Hill, Australia) and incubated at 37 °C for 60 min to permit the PBGD-catalyzed generation of hydroxymethylbilane ...
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bioRxiv - Microbiology 2022Quote: ... assays22 and nanostructure-initiator mass spectrometry (NIMS)23 assays were performed on the supernatant fraction and the remaining material was filtered using Miracloth (Millipore Sigma, Burlington, MA, USA) and dried at 50 °C to determine the biomass dry weight.
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bioRxiv - Microbiology 2022Quote: ... hormones were maintained in the culture medium throughout the course of each assay. 17-ß-estradiol (i. e., E2) and progesterone (i. e., P4) (both from Sigma; St. Louis, MO) were selected for use in infection studies because these hormones represent the major forms of estrogens and of progestogens (respectively ...
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bioRxiv - Pathology 2019Quote: Plasma samples from cohort A rhesus macaques were analyzed using Milliplex map Nonhuman Primate Cytokine Magnetic Bead Panel Premixed 23-Plex Assay (EMD Millipore Corporation, Billerica, MA). Assays were run on a Bio-Plex 200 system and analyzed using Bio-Plex Manager Software version 6.1.1 (Bio-Rad Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Physiology 2020Quote: ... and 120 minutes thereafter and blood glucose concentrations were measured by colorimetric assay (PGO enzyme preparation and dianisidine dihydrochloride, Sigma-Aldrich, St. Louis, MO).
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Tissues were homogenized in a modified radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1% protease inhibitor mixture and 1% phosphatase inhibitor mixture (Sigma-Aldrich, St. Louis, MO) and tissue lysates were resolved by SDS-PAGE ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed or tissue mechanically homogenised in lysis buffer (Extraction buffer, FNN0011) and protein content was quantified by a BCA assay (Sigma-Aldrich, St. Louis, MO). Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... leaf discs were placed in a 96-well plate containing 50 μL of distilled water and 50 μL of assay solution containing 20 mM Luminol (Sigma-Aldrich, St. Louis, A8511), and 1 mg/mL peroxidase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Bioengineering 2022Quote: ... total mRNA was measured using the RiboGreen assay with an upfront lysis step in 2% Triton X-100 (SX100-500ML; Sigma Aldrich, St. Louis, MO) to lyse the LNPs ...
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bioRxiv - Physiology 2022Quote: The subcellular distribution and possible spatial association between S-NO and Cx43 were evaluated by Proximity Ligation Assay(Soderberg et al., 2006) (Sigma, St. Louis, MO, USA). Tissue sections (6 μm ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were analyzed using MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 25 Plex - Immunology Multiplex Assay (EMD Millipore Corporation, Billerica, MA, USA). and custom FAS panel on the Luminex MAGPIX ...
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bioRxiv - Neuroscience 2022Quote: Brain punches were homogenized in radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase inhibitor cocktail 1 and 2 and protease inhibitor cocktail (Sigma Aldrich, Saint Louis, MO) followed by centrifugation at 16,000 g for 20 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Inflammatory proteins were measured using the MILLIPLEX Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex – Immunology Multiplex Assay (EMD Millipore. Corporation, Billerica, MA). Calibration curves from recombinant cytokine standards were prepared with threefold dilution steps in the same matrix as the samples ...
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bioRxiv - Biochemistry 2024Quote: ... Extracellular acidification rate (ECAR) measurements were collected and normalized to protein content that was determined by Pierce™ BCA Protein Assay (Sigma, Catalog number 23228). Glycolysis ...