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Citations for Millipore Sigma :
2401 - 2450 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Adult zebrafish were assayed for response to a mixture of 8 amino acids (Ala, Cys, His, Lys, Met, Phe, Trp, and Val; 0.1 mM each in distilled water; Sigma). No stimulation was provided for the first 5 mins following which the amino acid mix was introduced in one side of the tank at the rate of 1 ml/min using a syringe pump ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+ -NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... The culture media for the dividing cells (HI-10) consisted of RPMI-1640 medium + 10% heat inactivated FBS (Sigma), 100 μg/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Plant Biology 2022Quote: ... The transformants were then spotted on SD (−Trp, −Leu, −His) selection media containing 1 mM 3-amino-1,2,4-triazole (Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Immunology 2024Quote: ... whereas CoMiX-FHR4 molecules and VHH controls were detected with a mouse anti-His HRP-conjugated mAb (Sigma-Aldrich). The plate was revealed with OPD/H2O2 HRP-chromogenic mix substrate and the absorbance was measured at 492 nm ...
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bioRxiv - Biochemistry 2024Quote: ... with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV) and His-tagged constructs were cloned into a modified form of pRSF_Duet-1 (Novagen) with a TEV cleavage site following the hexahistidine tag ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtered through a 0.22 µm filter then applied to a 5 mL His-TrapTM HP (Sigma) column pre-equilibrated with the same buffer at a flow rate of 1.5 mL.min-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The 8 ml supernatant was applied to 1 ml packed volume of Ni-NTA His-Bind Resin (Millipore Sigma). Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... and digested in 4 mL of RPMI-1640/5% HI-FBS containing 1 mg/mL collagenase D (Sigma-Aldrich) and 30 μg/mL DNase I (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was clarified by centrifugation and the supernatant was applied to Ni2+-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and His wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression and native purification was performed by using His-select Nickel affinity gel resin column (Sigma Aldrich, USA) according to the manufacture’s instruction ...
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bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
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bioRxiv - Microbiology 2023Quote: An N-terminal His-tagged fusion of HcpGA1 was constructed by cloning QR305_02952 into the NdeI–BamHI sites of pET16b (Novagen), and the plasmid was transformed into E ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentrations of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported 21 ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana were tandem affinity-purified using a His-Bind resin followed by anti-Flag-M2-agarose beads (Sigma-Aldrich) as described32 with slight modifications ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 104 cells were used per poly-L-lysine (1:100 dilution with bidest, Millipore, # A-003-E) coated 10 mm coverslip ...
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bioRxiv - Neuroscience 2019Quote: ... P0 rat hippocampal neurons were plated to poly-D-lysine (PDL; 10μg/cm2; Millipore Cat# A-003-E) coated coverslips at 5×104 neurons per well in a 24-well plate ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Cell Biology 2020Quote: ... and chamberslides were in additions pre-treated with Poly-D-Lysine (Cat nb A-003-E, Millipore, Sigma) to increase adherence of the ECM ...
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bioRxiv - Microbiology 2021Quote: The anti-flavivirus E monoclonal antibody (mAb) D1-4G2-4-15 was purchased from Millipore (Billerica, MA, USA). The rabbit anti-ZIKV E polyclonal antibody was purchased from GeneTex (Alton PkwyIrvine ...
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bioRxiv - Cell Biology 2020Quote: ... The primers for PKC isoforms and E-cadherin were adopted from Awadelkarim et al.iv and Labernadie et al.v and ordered from Sigma. Prior to all experiments ...
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bioRxiv - Cell Biology 2020Quote: ... Spread screen samples were infected with VACV E-EGFP L-mCherry at MOI 0.02 and infection media was changed to media containing AraC (10µM, Sigma) at 8 hpi.
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bioRxiv - Genetics 2020Quote: ... 20 mM MgSO4, 200 mM HEPES, pH 7.9), protease inhibitor cocktail (AEBSF, 1,10-Phenanthroline, Pepstatin A, E-64) (Sigma), and TritonX-100 ...
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bioRxiv - Biochemistry 2020Quote: ... 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and α-tocopherol (vitamin E) was from Sigma Aldrich (St. Louis, MO, USA). TNF-α was obtained from Invitrogen (Carlsbad ...
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bioRxiv - Biophysics 2024Quote: ... E-cadherin knockdown was induced in shCDH1 cells by adding 200 μM isopropyl-β-D-thiogalactoside (IPTG; Sigma) 72 h prior to experiments.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in 1mL of Protease solution composed of 7.5mg/mL of protease E (P8811, Sigma Aldrich) diluted in dissociation medium [6mM CaCI2 ...
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bioRxiv - Cancer Biology 2024Quote: Formalin fixed paraffin embedded tissues were routinely stained with hematoxylin-eosin (H&E) (Sigma-Aldrich, St. Louis, MO), and immunohistochemistry was performed for E-cadherin and vimentin ...
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bioRxiv - Cell Biology 2023Quote: ... this was done by incubating embryos right after fertilization in 1.5 mg/mL Pronase E (Sigma-Aldrich, #107433) for approximately five minutes ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Routine hematoxylin and eosin (H&E) stainings and Periodic Acid Schiff (PAS) staining (Millipore-Sigma, catalog #395B-1KT) were performed per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... sectioned and stained with hematoxylin and eosin (H&E) or immunohistochemically stained for Tyrosine Hydroxylase (ab152, Millipore Sigma) or UCP1 (ab10983 ...
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bioRxiv - Cancer Biology 2023Quote: ... E-A14 and M-A14 cells and 10µg.mL−1 of Insulin-transferrin-sodium selenite media supplement (Sigma-Aldrich) for NEU15.
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bioRxiv - Cancer Biology 2023Quote: ... Sections of 4 µm were stained with haematoxylin and eosin (H&E, Sigma-Aldrich, St. Louis, MO, EUA) or antibodies against fibronectin (ab2413 ...
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bioRxiv - Cell Biology 2023Quote: ... 300,000 to 400,000 NPCs seeded into microscope coverslips were treated with 100nM compound E (EMD Millipore, Temecula, CA) in BrainPhys medium (StemCell Technologies ...
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bioRxiv - Pathology 2023Quote: Hair follicle parameters were stained with H&E (H3136 Sigma-Aldrich Hematoxylin and E4009 Sigma-Aldrich Eosin Y). Dermis-dermal adipocyte layer thickness and the numbers of hair follicles and anagen stages were counted in three areas (9 sections ...
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bioRxiv - Developmental Biology 2023Quote: ... The zona pellucidae of the GV zygotes and cleavage-stage embryos were removed with 0.5% pronase E (Sigma) and the cleavage-stage embryos were then incubated in Ca2+-free Chatot-Ziomek-Bavister (CZB ...
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bioRxiv - Cell Biology 2023Quote: ... 4 x 105 cells per well were plated in media containing 100 nM GSI (Compound E; Millipore, 565790) on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The signal was detected by the Pro e-BLOT Touch Imager using chemiluminescent substrate (WBKLS0500; Merck Millipore, Boston), and the band intensity was measured using ImageJ software (National Institutes of Health).
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p) and placed into a stereotactic frame (Kopf) ...
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bioRxiv - Neuroscience 2021Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Neuroscience 2020Quote: Mice were anaesthetized using Avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich, 476 mg/kg, i.p.) and were surgically implanted with a microdrive (manufactured with the assistance of the Advanced Manufacturing Support Team ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% glucose) or YPL (0.5% yeast extract, 2% peptone and 2% lactate (Sigma L1375)) ...
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bioRxiv - Bioengineering 2023Quote: Photoinitiator 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (HEPK, Sigma Aldrich) was dissolved into deionized water to obtain an approximately 0.077 wt% solution ...
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bioRxiv - Biophysics 2020Quote: ... biotin binding deficient subunits equipped with a C-terminal His6-tag – “dead” – and functional subunits with C-terminal 3C protease cleaving site followed by Glu6-tag – “alive” – were expressed as inclusion bodies in E.coli BL-21 using the pET expression system (Novagen). Inclusion bodies were dissolved in 6 M Guanidine hydrochloride at 10 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... the resin was incubated with 1 CV of 100 μg/ml 3x FLAG tag peptide (SIGMA F4799, Sigma-Aldrich) for 30 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or these cells stably transfected with human CB1 or CB2 receptors with 3x N-terminus haemagglutinin tags (AtT20-CB1 and AtT20-CB2respectively).17 Cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were obtained from commercial sources and used for immunoblots: anti-flag-tag (F3165, Sigma), anti-V5-tag (R960-25 ...