Labshake search
Citations for Millipore Sigma :
1901 - 1950 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from approximately 100mg flash-frozen liver tissue following homogenization in RNAzol RT reagent (Sigma-Aldrich). cDNA was synthesized from 1ug RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2022Quote: ... and incubated for 30 minutes at RT in a homemade Click reaction mixture containing 4 mM CuSO4·5H2O (Sigma), 4 μM Alexa Fluor-647-conjugated azide (ThermoFisher) ...
-
bioRxiv - Physiology 2022Quote: ... Cells were subsequently washed with PBS and incubated with 1.5 mg/ml glycine (Sigma-Aldrich, 10 min at RT) to quench unreacted paraformaldehyde followed by washing with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... the zebrafish larvae were permeabilized for 1h at RT with a blocking buffer [5% BSA (A9647-10G, Sigma-Aldrich), 5% sheep serum (013-000-1210 ...
-
bioRxiv - Plant Biology 2024Quote: ... After immunolabeling the coverslips were incubated in Acryloyl-X-SE 0.1 mg/mL in PBS (ThermoFischer) overnight at RT and then in the monomer solution (11.7% NaCl, 3 % acrylamide (Sigma), 0.15% N,N′-methylenebisacrylamide (Sigma) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were blocked with 5% BSA in PBS for 1 hour at RT and incubated overnight at 4°C with primary antibodies diluted in 0.5% BSA/1% Tween-20 (Sigma) in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... 4%) for 30 min at RT followed by a permeabilization step by using Triton X-100 (0.1%; Sigma-Aldrich) for 15 min at RT or Tween-20 (0.1% ...
-
bioRxiv - Molecular Biology 2024Quote: ... for 10 min at room temperature (RT) and permeabilized for 5 min in 0.5% Triton X-100 (#T9284, Sigma) with 2 mM VRC diluted in PBS on ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were incubated and inverted for 10 min at RT with ammonium chloride lyse [NH4Cl 155 mM (Sigma Aldrich), NaHCO3 10 mM (Sigma Aldrich) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... PFA was subsequently quenched with 500 mM NH4Cl for 10 min at RT and then cells were permeabilized with 0.1% Triton-X-100 (Sigma) for 10 mins at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... The signal was developed by incubation with DAB (3,3′-diaminobenzidine) for 10 minutes at RT and counterstained with hematoxylin (Sigma), followed by a wash in 0.02% ammonia water before mounting ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were initially blocked for 2 hours at room temperature (RT) with PBS-T 0.05% containing 10% of a donkey or goat Serum (Sigma). Primary antibodies ...
-
bioRxiv - Cell Biology 2023Quote: ... specimens were prepared in PBS and subsequently fixed for 4 h at RT in fixative (2% glutaraldehyde (Sigma, Germany)/ 4% paraformaldehyde (Merck ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the following changes: 90 minute primary antibody incubation at RT (rabbit α-GST, Sigma Aldrich #G7781, 1:4000) and 30 minute secondary incubation at RT (goat α-rabbit HRP ...
-
bioRxiv - Immunology 2023Quote: ... and fixed for 3 hr at room temperature (RT) in freshly prepared Carnoy’s fixative [60% anhydrous methanol (Sigma-Aldrich), 30% chloroform (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2023Quote: ... for 20 min in the dark RT and washed with 100 mM ammonium bicarbonate (NH4HCO3) (09830-500G, Sigma-Aldrich) and dehydrated as above ...
-
bioRxiv - Cell Biology 2023Quote: ... BMDMs were crosslinked with 0.4% paraformaldehyde for 10 minutes at room temperature (RT) and immediately quenched with 0.125 M glycine (Millipore Sigma) for 5 minutes at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... were added and incubated for 2 h at RT on shaker followed by 3x washes in 0.1% TritonX100 (Sigma) in PBS (Gibco).
-
Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (15,000 rpm, 10 min, RT) and the supernatants were transferred into glass vials containing MTBSTFA (70 μl, Sigma) for derivatization at 70 °C for 1 h ...
-
bioRxiv - Genetics 2023Quote: ... The seminiferous tubules were incubated for 60 min at RT in fixation buffer (100 mM HEPES pH7.4, 4 mM CaCl2, 2.5% glutaraldehyde, 2% PFA, all from Sigma Aldrich) and then the buffer was exchanged with fresh fixation buffer and the samples left overnight fixed at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated for 1 h at RT with secondary AP-conjugated anti-mouse or anti-rabbit antibodies (1:20,000, Sigma), washed three times for 5 min with PBT and once for 15 min in 20 mM Tris-HCl (pH 9.8 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed at RT for 10 min in 1 ml RIPA buffer containing 250 U/ml Benzonase (Sigma), 1X cOmplete EDTA-free protease inhibitor cocktail (Roche) ...
-
bioRxiv - Genetics 2024Quote: ... followed by incubation for 20 minutes at RT in the dark with 1 µl 50 mM chloroacetamide (Sigma-Aldrich). For tryptic digestion ...
-
bioRxiv - Genetics 2020Quote: ... and 1 μl of 1:10 diluted DNA was used as a template for PCR using the REDTaq ReadyMix PCR Reaction Mix (Sigma-Aldrich). The PCR conditions were as follows ...
-
bioRxiv - Microbiology 2019Quote: ... 5’-CTGGGAGTATACTTGTAGTC-3) were run in multiplex with PCR reaction mixtures consisting of 10 μl redtaq (REDTaq ReadyMix PCR Reaction Mix, Sigma-Aldrich), 2 μl DNA ...
-
bioRxiv - Biochemistry 2021Quote: ... the Msm guaB1 gene was amplified by PCR by primer pair 17/18 and inserted into the PCR-linearized pTriex-4 vector (Novagen, USA) with primers 15/16 by the In-Fusion approach ...
-
bioRxiv - Microbiology 2020Quote: ... the junctions of targeted integration were amplified by (semi-)nested PCR using 300 ng genomic DNA containing 1x PCR buffer (Sigma Aldrich), 1.5 mM MgCl2 ...
-
bioRxiv - Genomics 2021Quote: ... The number of necessary library PCR amplification cycles was determined by qRT-PCR using SYBR Green 10x in DMSO (Sigma-Aldrich) and NEBNext Master Mix 2x (New England BioLabs) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The modern DNA of each species was used for a long-range PCR (Sigma Aldrich Expand™ Long Template PCR System). Two primer pairs were used for the human mtDNA amplification (Gunnarsdóttir et al. ...
-
bioRxiv - Immunology 2023Quote: ... a targeted amplicon library was generated by PCR from the standard gene expression library cDNA using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004). 50uL PCR reactions were prepared by combining 25uL ReadyMix with 2.5 uL of a 10uM stock of each primer (10x Partial Read 1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... samples were rinsed several times in PBT and treated with imidazole 1M (Sigma, I5513)/PBT supplemented with H2O2 ...
-
bioRxiv - Microbiology 2022Quote: ... FAMEs were identified by comparison of their retention times with those of standards (Sigma) and by their mass fragmentation spectra ...
-
bioRxiv - Microbiology 2022Quote: ... Cysts were generated for indicated times and treated with established antiparasitics (pyrimethamine, sulfadiazine (Sigma)) and 1-hydroxy-2-dodecyl-4(1)-quinolon (HDQ ...
-
bioRxiv - Physiology 2021Quote: ... rAAVs were washed 3 times with PBS using Amicon Ultra Centrifugal Filter Units (Millipore) and resuspended in PBS + 0.001% Pluronic F68 ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were washed 3 times in PBS and fixed in 4% paraformaldehyde (Sigma Alrich) PBS for 20 minutes at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Slides with paraffin sections were washed three times in clean xylene substitute (Sigma A5597), then once each in 100% ethanol ...
-
bioRxiv - Molecular Biology 2019Quote: ... Hepatocytes were washed 4 times with ice-cold low glucose DMEM (D6046; Sigma-Aldrich) supplemented with 10 mM Hepes (#15630080 ...
-
bioRxiv - Plant Biology 2019Quote: ... Membranes were washed 5 times and revealed with Immobilon western chemiluminescent HRP substrate (Millipore).
-
bioRxiv - Cell Biology 2019Quote: ... washed 3 times in PBS before incubation in PBS supplemented with 3% BSA (Sigma) for 30 min to block non-specific antibody binding ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were washed three times for 5 min with 0.002 % NP40 (Sigma-Aldrich, i8896) in PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... They were rinsed three times with Hank’s Balanced Salt Solution (HBSS, Millipore Sigma, 55021C), then cut into 1mm3 pieces with surgical scissors ...
-
bioRxiv - Bioengineering 2021Quote: ... They were rinsed three times with Hank’s Balanced Salt Solution (HBSS, Millipore Sigma, 55021C), cut into 1mm3 pieces with surgical scissors ...
-
bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted ten times with IP-Lysis buffer supplemented with 15mM NEM (Sigma) and protease inhibitor cocktail ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were washed 2 times with PBS and fixed in 3.7% formaldehyde (Sigma Aldrich) at RT for 15 min ...
-
bioRxiv - Microbiology 2020Quote: ... washed with PBS three times and permeabilized with 0.2% Triton X-100 (Sigma, T9284)/PBS for 20 min at RT ...
-
bioRxiv - Microbiology 2020Quote: ... Wells were then washed three times with PBS containing 0.05% Tween-20 (Sigma-Aldrich), blocked by incubation overnight at 4°C with 200 μl of 3% nonfat milk-PBS/well and washed again the next morning ...
-
bioRxiv - Microbiology 2020Quote: ... Wells were then washed three times with PBS containing 0.05% Tween-20 (Sigma-Aldrich). One hundred microliters of secondary antibody (alkaline phosphatase-conjugated goat anti-rabbit IgG or goat anti-human IgG ...
-
bioRxiv - Systems Biology 2021Quote: ... cells were washed three times with PBS supplemented with 0.1% Tween-20 (Sigma-Aldrich) (PBS-T ...
-
bioRxiv - Plant Biology 2020Quote: ... and retention times were compared with authentic standards (Sigma Aldrich, St Louis, MO, USA) for volatile identification68.
-
bioRxiv - Immunology 2020Quote: ... washed three times with PBS and permeabilized with 2% Triton X-100 (Sigma-Aldrich) 5% Fetal Bovine Serum (FBS ...