Labshake search
Citations for Millipore Sigma :
2051 - 2100 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Regular Mycoplasma testing was performed using MycoLookOut PCR (Sigma). OVSAHO ...
-
bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned in the pET28b vector (Novagen) linearized with BamHI and NotI according to the sequence and ligation-independent cloning (SLIC ...
-
bioRxiv - Microbiology 2020Quote: ... KiCqStart SYBR green primers for qRT-PCR (Sigma-Aldrich) were specific for MCM2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Oligonucleotides for PCR amplification were obtained from Sigma Aldrich. For each construct the relevant open reading frame was completely verified by Sanger sequencing (GATC/Eurofins Scientific) ...
-
bioRxiv - Microbiology 2022Quote: Expand High Fidelity PCR System (Sigma-Aldrich, catalog #: 11759078001).
-
bioRxiv - Synthetic Biology 2022Quote: ... PCR primers were synthesized by Sigma Aldrich (S2 Table), longer DNA fragments and genes were ordered from GeneScript (S3 Table) ...
-
bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
-
bioRxiv - Microbiology 2020Quote: ... PCR primers and oligos were purchased from Sigma-Aldrich. Restriction enzymes were purchased from New England Biolabs ...
-
bioRxiv - Microbiology 2020Quote: ... The purified PCR product and pET-28a(+) (Novagen, Germany) were digested with BamH I and Xho I (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were then cloned in the pET15b (Novagen) plasmid modified compatible with GatewayTM cloning strategy ...
-
bioRxiv - Cancer Biology 2022Quote: ... Regular mycoplasma testing was performed using MycoLookOut PCR (Sigma). When were they last tested? HGSOC lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
-
bioRxiv - Cell Biology 2024Quote: ... All PCR was performed using KOD polymerase (EMD Millipore). To generate pPM09 ...
-
bioRxiv - Microbiology 2023Quote: ... Colony PCR was performed using REDtaq Ready Mix (Sigma) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... were amplified by PCR using KOD Xtreme polymerase (Novagen) using primers (Table S5 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The PCR products were run in 2% agarose (SIGMA) gels and visualised under UV light.
-
bioRxiv - Microbiology 2020Quote: ... The bacterial solution was then incubated at room temperature (RT) for 20 min under moderate shaking in the presence of 0.5 mg/mL lysozyme (Sigma-Aldrich) at and additional 10 min after addition of 0.1 mg/mL DNase ...
-
bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
-
bioRxiv - Cell Biology 2020Quote: ... in PBS for 30 min before incubation with primary antibodies for 1 h at RT in the permeabilization buffer supplemented with 0.5% normal goat serum: TFE3 (0.6 μg/ml; Sigma-Aldrich, HPA023881), LAMP1 (1/50 conditioned medium of hybridoma cells ...
-
bioRxiv - Immunology 2021Quote: ... The H1568 cells were then fixed in 4% formaldehyde solution in PBS for 30 min at RT and then permeabilized with 0.2% (v/v) Triton X-100 (Sigma-Aldrich) in PBS for 10 min at RT ...
-
bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally detached from the mask and incubated for 1 hour at RT with a 5 μg/mL solution of fibronectin (Sigma) in NaHCO3 100 mM ...
-
bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
-
bioRxiv - Molecular Biology 2021Quote: ... The brain sections were dried for 20 minutes at RT and mounted on 0.1% gelatin-coated slides with FluoroshieldTM with DAPI (Sigma-Aldrich). Sections were visualized and photographed using a Zeiss Axio Imager Z1 microscope equipped with an Axiocam MR3.0 camera and Axivision 4.9.1 software ...
-
bioRxiv - Biochemistry 2019Quote: ... The sample was split in half and incubated for 1h at RT (while rotating) with either anti-FLAG M2 affinity gel (Sigma) or anti-HA affinity matrix (mAb 3F10 ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... the cells were washed and incubated in CCF4 for 1 h at RT in the presence of 2.5 mM probenecid (Sigma-Aldrich). Live cells (propidium-iodide negative ...
-
bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
-
bioRxiv - Neuroscience 2021Quote: ... 4% in PBS for 15 min at RT] and subjected to staining with filipin (40 μg/mL in PBS for 2 h at RT; from 250-fold ethanolic stock solution; F9765, Sigma). Fluorescence images of cultured cells were acquired using an inverted microscope (Axiovert 135TV ...
-
bioRxiv - Immunology 2021Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of pre-reverse transcription (RT) lysis mix containing 0.1 μl of NP40 (Sigma-Aldrich), 0.5 μl of RNAse Inhibitor (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS with 0.05% Tween-20 for 1 h at RT before addition of primary antibodies (SRRM4, HPA052783, Sigma-Aldrich Quimica S.A. ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 14 days of differentiation with 4% paraformaldehyde in PBS for 10 min at room temperature (RT) and immunofluorescence staining was performed with βIII-tubulin (1:5,000 dilution, Sigma, T8660), NF200 (1:6,000 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... for 10 min at RT and then stained with a primary antibody (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556 ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2020Quote: ... in Tris-buffered saline (TBS) for 15 min at RT and then further treated with 0.1% Triton-X-100 (Sigma-Aldrich) and 2% goat serum in TBS overnight at 4°C for improving antibody penetration ...
-
bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... PBST) for 1 h at RT followed by incubating with primary antibodies (anti-PV, 1:1000-2000, P3088, Sigma-Aldrich) in 3% NGS in PBST at 4 °C overnight ...
-
bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were treated with a 10 min RT incubation in 0.1% Triton-X 100 (Sigma, St. Louis, MO, Cat. # X100) to permeabilize cells ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were then permeabilized with 0.1% Triton X-100 for 15 min at RT and blocked with 1% bovine serum albumin and 5% goat serum (Sigma). cells were incubated with primary antibodies Esrrb (PP-H6705-00 ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were grown to near confluency on 6 cm dishes and total RNA was extracted using the RNAzol RT reagent (Sigma) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: Cells grown on coverslips (1.5#) were incubated for 20 minutes at room temperate (RT) in PBS with 4% paraformaldehyde (Sigma-Aldrich) and washed three time for 10 minutes with PBS ...
-
bioRxiv - Genomics 2022Quote: ... the slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (Sigma–Aldrich) in antifade DABCO (Sigma–Aldrich).
-
bioRxiv - Molecular Biology 2021Quote: ... Germany) slides were incubated for 1h at room temperature (RT) in TBS containing 10% donkey serum (Sigma-Aldrich, Vienna, Austria), 1% bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2020Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of a pre-reverse transcription (RT) lysis mix containing 0.1 μl NP40 (Sigma-Aldrich), 0.5 μl RNAse Inhibitor (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2020Quote: ... After rinsing sections were incubated 10 min at room temperature (RT) with 0.001 % DAPI (4’,6-diamidino-2-phenylindole dihydrochloride, Sigma, D9542) in PBS for nuclear staining ...
-
bioRxiv - Biophysics 2021Quote: ... After gelation for 2 h at RT in a humidified chamber the samples were transferred into digestion buffer (8 U/ml Proteinase K (P4850, Sigma), 50 mM Tris pH 8.0 ...
-
bioRxiv - Neuroscience 2020Quote: ... Samples were then washed 2 x 1 h in 100% MeOH followed by overnight incubation at RT in DiChloroMethane:MeOH (DCM, Sigma – 270997) 2:1 ...