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Citations for Millipore Sigma :
1701 - 1750 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and fixed 10 minutes at RT in fixation buffer (10% formaldehyde solution, Sigma Aldrich, in nuclease free water). Cells were washed 2 x and permeabilized O/N in 70% ethanol at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 15 min at RT then permeabilised using 0.5% saponin (Sigma-Aldrich) and stained for 30 min at 4°C for the following intracellular markers ...
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bioRxiv - Neuroscience 2022Quote: ... containing coverslips were fixed for 20 min at room temperature (RT) in 4% (w/v) paraformaldehyde (PFA, Sigma) in 0.15 M phosphate buffer (potassium dihydrogen phosphate (Merck ...
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bioRxiv - Pathology 2023Quote: Liver and renal cortex RNA from EU-exposed mice were extracted using RNAzol RT reagent (Sigma-Aldrich, # R4533). The incorporated EU was detected by click chemistry biotinylation and dot blotting ...
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bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592 ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min at RT and then permeabilized for 3 minutes with 0.2% Triton-X 100 (Millipore Sigma). Over-extracted cells were treated with 0.4% Triton-X 100 for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was then subjected to the following washes at RT with agitation: 125 mM SB-10 (Sigma D4266) in 50 mM sodium/10 mM phosphate buffer (VWR BDH9286 ...
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bioRxiv - Cell Biology 2023Quote: ... optionally pre-coated at RT for 20min with 20μl of 0.2mg/ml in DDW concanavalin A (L7647, Sigma) and air-dried.
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416 ...
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bioRxiv - Molecular Biology 2023Quote: ... staining, immunoselected transcripts were on-bead digested (10 min, RT) with 2 µL 10 µg/mL RNaseA (Sigma), separated by UREA-PAGE ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Immunology 2023Quote: ... during 5 minutes at RT followed by blocking with 10 μg/mL human γ-globulin (Sigma-Aldrich, USA) in TBS-0.5% blocking reagent (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA extraction was performed using the RNAzol ® RT reagent following the manufacturer’s recommendations (Sigma-Aldrich, USA). After the DNAse treatment with RQ1 DNAse (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in 66% dichloromethane/33% methanol for 3h at RT and finally cleared in ethyl cinnamate (Sigma 112372). Primary antibodies used were as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... for 30 min at RT and then permeabilized with a solution of 0.5% Triton X-100 (Sigma-Aldrich) and 3 mM EDTA (pH 8 ...
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bioRxiv - Neuroscience 2024Quote: ... in DPBS for 60 min at RT and incubated with primary antibodies against MAP2 (1:100, Sigma, 9942), GFAP (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed two times with PBS and fixed using 4 % formaldehyde (Sigma) in PBS for 5 min ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The plates were washed 3 times with 0.05% Tween-20 (P2287, Sigma-Aldrich) in PBS ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed (5 times with PBS-T) and AP substrate (Sigma) was added ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed three times with PBS + 0.1% Tween-20 (Millipore Sigma) and subsequently incubated with secondary antibodies (listed below ...
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bioRxiv - Cell Biology 2022Quote: ... Cotyledons were collected at different time points and mounted in perfluorodecalin (Sigma Aldrich) for confocal microscopy (Littlejohn et al. ...
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bioRxiv - Biophysics 2022Quote: ... the membranes were washed three times with phosphate-buffered saline (PBS, Sigma-Aldrich) and stored in PBS at 4°C until further use ...
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bioRxiv - Microbiology 2019Quote: ... We diluted pure toluene 1000 times in 2,2’,4,4’,6,8,8’-heptamethylnonane (Sigma Aldrich) and added 0.2 ml of this solution to each vial with 1 ml bead suspension ...
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bioRxiv - Neuroscience 2019Quote: ... Cleared sections were washed overnight three times with 0.2% Triton-X100 (T8787, Sigma) in PBS on a shaker at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell number was measured at the indicated time points using sulforhodamine B (Sigma) staining ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were washed three times with PBS plus 0.1% Triton X-100 (Millipore) and incubated with appropriate secondary antibody (1:100 anti-mouse IgG3 Alexa 488 and 1:300 goat anti rabbit Alexa 568 ...
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bioRxiv - Bioengineering 2021Quote: ... washed multiple times in PBS and permeabilized with 0.1% Triton-X (Sigma-Aldrich) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times in PBS and resuspended in PBS + Protease Inhibitor (PI, Sigma). Worm ‘popcorn’ was prepared by dripping worm solution into liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed at indicated time points post degranulation using RIPA buffer (Sigma) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed in PBS three times and permeabilized in 0.1% saponin (Sigma) and blocked in 1% BSA (USBiological Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... mice were exposed three times to an OVA (OVA grade V, Sigma-Aldrich) aerosol (1% wt/vol in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pre-treated for the indicated times with PitStop 2 (Sigma, SML1169), Dyngo 4a (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... rinsed several times with H2O followed by Reynolds lead citrate in H2O (Sigma) for 10 min and rinsed several times with H2O ...
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bioRxiv - Plant Biology 2021Quote: ... rinsed 2 times and blocked with 4% (w/v) bovine serum albumin (Sigma) for 1h ...
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bioRxiv - Biophysics 2020Quote: ... for 15 min and washed three times in PBS (Sigma-Aldrich, D8537-500ML).
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bioRxiv - Cancer Biology 2020Quote: ... at the time of cell seeding or 5mg/mL doxycycline (Sigma-Aldrich D9891) 48 hours prior to cell seeding ...
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bioRxiv - Cancer Biology 2021Quote: ... beads were washed three times in the buffer containing 8M urea (U5378, Sigma),0.1 M NaxHxPO4 pH 6.3 ...
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bioRxiv - Biochemistry 2022Quote: ... The lipid film was further washed three time with n-pentane (Sigma-Aldrich) and lyophilized overnight ...
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bioRxiv - Biochemistry 2022Quote: ... and then washed three times with Dulbecco’s phosphate buffered saline (DPBS, Sigma-Aldrich). The tissues were incubated in collagenase type L (2 mg/mL solution in basal medium ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were then washed three times with PBS + 0.1% Tween-20 (Millipore Sigma) and subsequently incubated with secondary antibodies (listed below ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed 3 times in PBS and then mounted with Fluoshield (Sigma). Whole brain images were obtained on an Olympus virtual slide microscopy VS120-L100-W with a 10x objective (Olympus Canada Inc. ...
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bioRxiv - Biophysics 2022Quote: ... the gels were rinsed 4 times with a 10mM HEPES (1M, Sigma-Aldrich) solution with pH in the range 8.2-8.4 and incubated with a 50 mg/ml dilution of PEG-SVA (Laysan Bio ...
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bioRxiv - Plant Biology 2021Quote: ... The beads were washed three times with phosphate buffered saline (PBS, Millipore Sigma). Proteins bound to beads were dissociated by incubation in 100 μL of Novex™ Tris-Glycine SDS sample buffer (2x ...
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bioRxiv - Cancer Biology 2020Quote: ... 25 million cells per time point was crosslinked with 1% formaldehyde (Sigma F8775) for 10 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... Samples were washed three times with PBS and incubated with DAPI (Sigma, B2883) for 1 minute ...