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Citations for Millipore Sigma :
1851 - 1900 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)– HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Cell Biology 2022Quote: ... for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8, 1:2,500, 05-949) for 6xHIS::NMY-2 detection ...
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bioRxiv - Molecular Biology 2023Quote: ... Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody, A3682, Sigma Aldrich). All three blots were developed for 9.2 s after adding SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Bioengineering 2023Quote: ... Detection of IgG1 employed anti-human IgG (VH+VL) (Jackson) and of IgA2 anti-human IgA (Hc only) (Sigma). Commercial antibodies were used at dilutions recommended by manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... gondii antibodies detection was conducted using an anti-monkey IgG FITC conjugate produced in rabbit (Sigma-Aldrich®, USA). T ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were electrophoresed on a 10% denaturing gel (or 7,5% for Rad53 detection) of 37,5:1 Acrylamide:Bis-acrylamide (Sigma-Aldrich #A3699). Proteins were then transferred to a nitrocellulose membrane (Amersham Protran 0.45 NC ...
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bioRxiv - Immunology 2023Quote: ... PLA was carried out with Duolink® in situ detection reagents following the manufacturer’s instructions (Millipore Sigma; MA, USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Developmental Biology 2024Quote: Proximity ligation assay (PLA) was carried out with Duolink® In Situ Detection Reagents Far Red (Sigma Aldrich, #DUO92013) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBS, resuspended in 100 μl of freshly prepared EdU detection reaction buffer (PBS, 2 mM CuSO4 (Sigma, C7631), 0.05 mM Biotin-Azide (Molecular Probes ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated for 30 min at RT in incubation buffer that consisted of 2% BSA (Sigma Chemical Co.) and 0.2% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... during 2 hours at RT and then rinsed in PBSDT (PBS 1x, 0.01% DMSO, 0.1% Triton x100 (Sigma, T9284)) for use in the week ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated for 1.5 hours at RT with an antibody against Hp1α (Merck Millipore, MAB3584, 1:500 dilution) and RNA Polymerase II (Santa Cruz ...
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bioRxiv - Cell Biology 2022Quote: Individual cells were sorted into 96-well plates containing 4μL of lysis buffer and index RT primers (0,2% Triton (Sigma Aldrich), 0,4 U/μL RNaseOUT (Thermofisher Scientific) ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Cells were cultured for 24 h and intracellular RT activity was determined with colorimetric reverse transcriptase assay (Sigma Aldrich) according to manufacturer’s instruction.
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bioRxiv - Physiology 2019Quote: ... the serial sections were incubated at RT in a secondary antibody conjugated with FITC (F0257; 1:100; Sigma-Aldrich) for 1 hr ...
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bioRxiv - Neuroscience 2021Quote: ... and the next day with laminin for 1h at RT (5µg/mL in distilled water) (Sigma-Aldrich, MO, USA). Neuronal cells were plated directly on top of the electrodes and treated with EVs or recombinant proteins ...
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bioRxiv - Bioengineering 2021Quote: ... Tissue was digested for 1 hr at RT with collagenase I (0.5 mg ml-1) (Sigma, cat. no. C9697). Digested tissue was rinsed with supplemented Schneider’s medium and finally resuspended in supplemented Schneider’s medium ...
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bioRxiv - Neuroscience 2020Quote: ... cells were permeabilized for 20 min at RT with DPBS + 0.1% Triton X-100 (Sigma, Cat N° T8787-250ML) followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at RT, membranes were developed with ECL substrate (10% Tris HCl 1M pH 8.5, 0.5% coumaric acid (Sigma-Aldrich), 0.5% luminol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Both fresh frozen and perfused sections were then permeabilized at RT for 20 minutes in PBS containing 0.05% Triton X-100 (Sigma). Sections were then blocked at RT for 2 hours in PBS containing 10% normal horse serum (NHS) ...
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Mechanistic model of MAPK signaling reveals how allostery and rewiring contribute to drug resistancebioRxiv - Systems Biology 2022Quote: Cells were fixed in 4% PFA for 30 min at room temperature (RT) and washed with PBS with 0.1% Tween- 20 (Sigma) (PBS-T) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated for 1 hour at RT in PBS containing 0.1% Triton X-100 (Sigma-Aldrich, PBS-T) and 10% normal donkey (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at RT followed by 0.1 M PBS washes and developed with 3,3-diaminobenzidine (DAB, Sigma-Aldrich). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were first incubated for 1h at room temperature (RT) in a blocking solution containing 1% BSA (Sigma), 2% Donkey Serum (Biowest ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at RT and subsequently incubated with primary antibodies: pan-GPR56 (clone H11) (Millipore MABN310, 1:150) and HA-Tag (3F10 ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 % of FBS for 1 h at room temperature (RT) and incubated with PSD95 (1:1.000, MAB1598; Millipore), PrP (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were then incubated 1h at RT with the rabbit polyclonal antiTWIST1 (1/50, ABD29, Millipore, Burlington; MA, USA); or 1h at 37°C with the sheep polyclonal anti-TWIST2 (1/200 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was dried overnight at RT and blocked for 30min with 2%(W/V) BSA in PBS (Sigma), incubated while shaking ON at 4°C with rabbit anti-Histone H3(Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... HUVEC were fixed with 4% PFA for 10 min at RT and permeabilized with 0.1% Triton X-100 (T8787, Sigma) for 10 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour at RT followed by 0.1 M PBS washes and developed with 3,3-diaminobenzidine (DAB, Sigma-Aldrich). Finally ...
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bioRxiv - Zoology 2020Quote: ... in a dark chamber at RT after which each slide was counterstained with 0.5% Bismarck Brown Y (Sigma, USA) before dehydration ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 1 ml of fresh media and treated with CCCP (0.1 mM, RT for 5 min, Sigma #C2759) for PMF decoupling ...
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bioRxiv - Microbiology 2020Quote: ... The culture medium containing the secreted proteins was harvested by centrifugation (5000 g for 20 min at RT) and filtered through a 0.22 µm membrane filter (Millipore). Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Cancer Biology 2019Quote: Cells were fixed in 4% PFA for 30 min at room temperature (RT) and washed with PBS with 0.1% Tween-20 (Sigma) (PBS-T) ...
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bioRxiv - Molecular Biology 2019Quote: ... Culture medium containing the secreted fSmChE proteins was harvested by centrifugation (5000 g for 20 min at RT) and filtered through a 0.22 μm membrane filter (Millipore). Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Physiology 2020Quote: ... Slides were then washed twice in warm water for 5 min and stained for 1h at RT with Picro-Sirius Red solution (0.1% (m/v) Sirius Red (Sigma) in saturated aqueous solution of Picric Acid (LabChem)) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were derivatized in RT for 3 min with 10 μl of 60% p-toluenesulfonyl isocyanate (PTSI) (Sigma Aldrich) in ACN (Hu et al. ...
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bioRxiv - Cell Biology 2021Quote: ... left blocking overnight at RT and gentle shaking in PBST supplemented with 5% BSA (Sigma-Aldrich, Catalog #A9647-100G), and washed once the next morning with 15 mL PBST for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples were blocked and permeabilized for 1 h at rt in PBS containing 0.3% TritonX‐100 (9002‐93‐1, Sigma), 5% normal goat serum (005‐000‐001 ...
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bioRxiv - Biochemistry 2021Quote: ... The wells were blocked for 30 min at RT with 15 μl PBS+1% BSA (filter sterilized, Sigma Aldrich). The primary antibody solution was prepared in PBS + 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... slices were blocked for 1 h 30 min at room temperature (RT) in a solution containing 0.3% Triton X-100 (SIGMA) and 5% Normal Goat Serum (NGS ...
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bioRxiv - Developmental Biology 2021Quote: ... plates were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Sigma-Aldrich, 5µg/ml in PBS, 1h at RT).
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bioRxiv - Immunology 2020Quote: ... Sections (3µm thick) were then incubated for 40 minutes at RT with 1:1000 dilution of anti-APE1 (HPA002564; Sigma). Detection of primary antibody was performed using the Super-sensitive–Polymer HRP system (Biogenex ...
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bioRxiv - Immunology 2021Quote: ... Qβ(1668)-VLPs were then derivatized for 30min at RT using dibenzocyclooctyne NHS ester (DBCO cross-linker) (Sigma-Aldrich) in a 5-fold molar excess ...
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bioRxiv - Immunology 2021Quote: ... Staining was performed for 1 hour at RT and slides were mounted with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich). Confocal images were acquired on an LSM 700 system (Carl Zeiss ...
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bioRxiv - Microbiology 2020Quote: ... COS-7 cells on glass coverslips were transfected as above and fixed at 24 hours post-transfection in 4% paraformaldehyde for 10 min at room temperature (RT) followed by permeabilization with 0.2% Triton X-100 (Sigma) for 5 min ...