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Citations for Millipore Sigma :
2101 - 2150 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The cells were then incubated with primary antibodies at RT for 2 hrs before proceeding with Duolink PLA (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Blots were blocked with 5 % milk for 1 h at RT and then incubated in the anti-FLAG M2 (Sigma) primary antibody at a dilution of 1:2500 at 4 °C overnight with agitation ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using RNAzol RT (Astral Scientific) per manufacturer’s instructions with the use of molecular grade isopropanol and ethanol (Sigma Aldrich) for RNA precipitation ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Bioengineering 2021Quote: ... cells were fixed with 3.6% (v/v) PFA for 15 min at RT in PBS and pellet sections stained with Alcian blue (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The fixing solution was removed together with the overlay after a 24-hour incubation at RT and plaques visualized by staining the monolayer with 0.1% crystal violet (#C0775; Sigma- Aldrich) diluted in a 20% ethanol (200 proof ...
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bioRxiv - Cancer Biology 2019Quote: ... Tissues were washed 5 times in PBT and incubated for 2h at RT with secondary antibodies and 4’,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma) (1µg/mL) ...
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bioRxiv - Immunology 2019Quote: ... 0.1% [w/v] saponin in PBS) for 25 min at RT and then stained with mouse anti-flavivirus antibody (clone D1-4G2-4-15, Millipore) diluted 1:500 in FACS wash buffer for 90 min at RT ...
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bioRxiv - Bioengineering 2019Quote: ... for 20 min at room temperature (RT) followed by 3 x 5 min washes with PBS/0,1% Triton-X 100 (Sigma-Aldrich). Plates were then incubated with the respective primary antibody in PBS/0,1% Triton-X 100/1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were permeabilized with 100% methanol at -20ºC for 3 min at RT and blocked with 2.5% normal serum from donkey (Jackson ImmunoReasearch, 017-000-121) or goat (Sigma, 19H092) in 1%BSA/PBS for 1h at RT ...
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bioRxiv - Molecular Biology 2021Quote: Cells were fixed using 4% paraformaldehyde (PFA) for 10min at RT and permeabilized with 0.1% Triton x-100 (Sigma, T8787) for 10min at RT ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... after which the cells were fixed with 10% formaldehyde for 20 minutes at RT and stained with Oil-red-O (Sigma). For chondrogenesis ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Immunology 2020Quote: ... for 1h at RT or addition of AP-conjugated goat anti-mouse IgM or IgA (Abcam and Sigma-Aldrich, respectively) and addition of pNPP substrate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then blocked for 1 hour at RT using 5% milk in TBS with 0.025% Tween 20 (Sigma, P1379) (TBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... for 15 min at room temperature (RT) with gentle shaking followed by washing in 1x PBS 0.3% Triton (Sigma #T9284) (PBS3T ...
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bioRxiv - Neuroscience 2022Quote: Coronal brain sections were permeabilized for 30 min at RT with 0.5% TritonX-100 (#66831, Carl Roth) in PBS and blocked for 30 min at RT with 0.2% BSA (#A7030, Sigma-Aldrich) and 5% donkey serum (#017-000-121 ...
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bioRxiv - Cancer Biology 2022Quote: ... were treated for 20 min RT with Poly-L-lysine solution 0.1 % (w/v) in H2O solution (cat# P8920 from Sigma-Aldrich). After two washes with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed (3 × 5 min. in PBS at RT) before staining with DAPI (1:1000; Sigma Aldrich, D9542) and AlexaFluor-488 Phalloidin (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted to 60 ng/μl and used in RT-qPCR using SYBR Green Jump Start Taq (Sigma-Aldrich) with 200 nM Forward and Reverse primers (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... approximately 1×108 cells were harvested (1400 g, 10 min, RT) and transferred to Hank’s balanced salt solution (Sigma Aldrich). The protein concentrations of the samples were determined by the BCA assay kit (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then washed in 1X PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Microbiology 2022Quote: ... 5 A260 units of the cleared cell lysates were incubated either for 30 min at RT either with 3 µg of micrococcal nuclease (Sigma), or 300 units of RNase T1 (Fermentas ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... in PBS for 5 min at RT and incubated with 5% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO) for 1h at RT ...
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bioRxiv - Neuroscience 2022Quote: NKCC1-HA-Flag-mVenus membrane expression and clustering was assessed with staining performed after a short fixation at room temperature (RT) in paraformaldehyde (PFA; 4% w/v; Sigma) and sucrose (20% w/v ...
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bioRxiv - Neuroscience 2022Quote: ... and then washed with PB (3 × 5 min; RT) and finally mounted on gelatinised slides and coverslipped with Mowiol DABCO (Sigma). For brightfield microscopy ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma ...
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bioRxiv - Immunology 2022Quote: ... 10 µM sections were cut and permeabilized for 60 mins at RT with 2% Triton X in PBS (Millipore Sigma). Slides were stained at 4°C overnight with a cocktail of anti-mouse CD4 PE (RM4-5) ...
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bioRxiv - Neuroscience 2022Quote: ... then secondary antibodies were applied for one hour at RT at indicated concentrations with 5 mg/mL DAPI (Sigma, D9542) applied at 1:5000 concentration as nuclear counterstain ...
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bioRxiv - Molecular Biology 2024Quote: ... washed twice with 800 µL IP buffer and incubated (5 min, RT) in the absence or presence of 10U benzonase (Millipore). Coenriched ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% Tween-20 (TBS-T) and incubated for one hour at RT with Mouse anti-FLAG (M2) antibody-HRP conjugated (Sigma) diluted 1:4000 in blocking milk ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed with PBS and permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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bioRxiv - Cell Biology 2023Quote: ... permeabilised with 0.3% Triton X-100 for 10 min and blocked for 1 h at RT with 2% bovine serum albumin (BSA, Sigma)/2% normal goat serum (GS ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed three times with 0.1% PBST and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary antibody (Cat No. NA934, Merck Millipore) diluted 1:1,000 in 0.05% PBST ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then blocked 1 hr at the room temperature (RT) in PBS with 2% Triton-X100 and 2% normal donkey serum (Sigma), followed by an incubation overnight at 4℃ with primary antibody diluted in the blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were first stained for 20 min at RT with extracellular antibodies in the staining mix containing FACS buffer (dPBS (Sigma) with 2% HI FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... and stained with a 1:5000 dilution of DAPI in PBS for 5-10 minutes at RT (DAPI was purchased from SIGMA). Alternatively ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were dehydrated with methanol/PBS and incubated O/N at RT in 66% dichloromethane (DCM; cat. #: 270947; Sigma-Aldrich) and 33% methanol ...
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bioRxiv - Immunology 2023Quote: ... IL10 and normalization control GAPDH were examined by RT-qPCR using gene specific primers (Sigma Aldrich, St. Louis, MO, USA) as previously described 11 ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 hr at room temperature (RT) and incubated with mouse monoclonal anti-aggrecan neoepitope antibody (10 µg/ml; clone AF-28, MAB19310-C, Millipore) and rabbit polyclonal anti-α-tubulin antibody (2 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 1:200 during 1 hour at RT or MYH11 (Prestige Antibodies power by Atlas Antibodies, Sigma Aldrich, cat. nr HPA015310) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Bound material was eluted (1h at RT, shaking at 850 rpm) from anti-FLAG M2 magnetic beads (Sigma, Cat. # M8823) with homemade 10 µg/µL Strep-6xFLAG-peptide in PBS supplemented with 0.1% glyco-diosgenin ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... the membranes underwent a 30 min-blocking at RT using 1x carbo-free blocking solution (Vector) containing 1% Tween-20 (Sigma). The membranes were then blotted with 1 μg/mL biotinylated Sambucus Nigra Lectin (SNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 12 min at room temperature (RT) and quenched by replacing the fixation solution with freshly made 0.2% NaBH4 (Sigma Aldrich) in PBS for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at room temperature (RT) and then blocked in 0.2% Triton X-100 and 10% ChemiBLOCKER (Millipore, #2170) for another 2 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were rinsed with PBS and then fixed in PBS for 20 min at room temperature (RT) with 1.6% paraformaldehyde (P6148, Sigma-Aldrich). Cells were then rinsed with PBS and then permeabilized with 0.5% Triton X-100 (T8787 ...