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Citations for Millipore Sigma :
2151 - 2200 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... lung sections were washed twice with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 min at RT and incubated in primary antibody mouse anti-laminA (Sigma Aldrich) at 1:500 overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1h at RT in blocking buffer (BB: 10% Bovine Serum Albumin 0.3% Triton™X-100 (Sigma-Aldrich) in 1X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% RNAse-free Bovine Serum Albumin for 30 minutes at RT before being incubated with primary antibodies (Thermofisher, mouse anti-tubulin, 1:1000; Sigma mouse monoclonal anti-myc clone 9E10 ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent a 2h incubation at room temperature (RT) in a blocking solution consisting of 0.1M PBS supplemented with 5% (v/v) goat serum (Sigma; G9023) and 0.3% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by incubation for 90min at room temperature (RT) with secondary antibody anti-goat or anti-rabbit IgG Biotin (Sigma) diluted 1:400 or 1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked for 1 h at RT in 5% BSA (Carl Roth, 8076.5) in Tris-buffered saline tween (TBST) buffer (0.15 M NaCl (Sigma-Aldrich, S7653), 60 mM TRIS base (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were permeabilized with 0.5% Triton-X100 in PBS for 5 min at RT and a blocking solution (PBS supplemented with 5% BSA (Sigma) and 0.1% Triton-X100 ...
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bioRxiv - Neuroscience 2024Quote: ... Lysates were spun at 5.000 g for 20 min at RT and loaded onto an OptiPrep iodixanol gradient (Sigma-Aldrich) (60% ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated in primary antibody for 12-18 hours at RT (goat anti-ChAT, 1:500, Millipore Sigma and Cat# ...
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bioRxiv - Biophysics 2020Quote: The scaffolds were rinsed with PBS three times and fixed overnight with 10% formalin (Sigma). Following formalin fixation ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed (5 times with PBS-T) and developed with either AP substrate (Sigma) and read at 405 nm (AP ...
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bioRxiv - Genetics 2021Quote: ... Coverslips were rinsed 3 times in PBS and mounted on glass slides with Fluoromount (Sigma) with DAPI (4′,6-diamidino-2-phenylindole).
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bioRxiv - Microbiology 2021Quote: ... three times with TBS and once in TBS containing 1% Triton X-100 (Sigma-Aldrich). The cells were then re-suspended in 5% BSA diluted in TBS for blocking 1 h at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL]; Sigma, Cat# F4799). The crosslinking was reversed overnight at 65 °C and purified DNA (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... ECs were then washed three times with PBS and permeabilized with 0.5% Triton-X (Sigma) for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... They were washed three times in PBST 0.3% (PBS, 0.3% Triton X-100 (T9284, Sigma), 10 minutes for each wash ...
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... ECs were then washed three times with PBS and permeabilized with 0.5% Triton-X (Sigma) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 3 times with PBS containing 0.05% Tween-20 (Sigma-Aldrich), blocked with 150 mL ELISA/ELISPOT Diluent (Life Technologies Corporation ...
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bioRxiv - Developmental Biology 2019Quote: ... the membrane was washed 3 times in water and immersed in 0.1% methylene blue (Sigma), 0.1M sodium acetate stain solution for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... the Cy3-DNA-SWCNTs were filtered 3 times using 100 kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Synthetic Biology 2020Quote: ... N,N,N’,N’-tetramethylethylenediamine (TEMED; Sigma-Aldrich T9281; 0.0025 times the weight of AAm) to form a fast-curable pre-gel solution ...
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bioRxiv - Physiology 2019Quote: ... They were then washed three times in PBS before blocking in 3% BSA (A8806; Sigma) in PBS for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... The prepared samples were washed at least three times in PBS (Sigma-Aldrich, D8537-500ML) prior to imaging.
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed several times and analyzed with chemiluminescence using ECL Lumina Forte (Millipore, WBLUF0500) using the Chemidoc XRS Imager (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatants were concentrated 10 times in an Amicon ultra 10K centrifugal filters (Millipore, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... washed 3 times in PBS and then permeabilized with 0.2% Triton™ X-100 (Sigma) in PBS for 3 min ...
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bioRxiv - Neuroscience 2021Quote: Around 30 5 dpf larvae or 5 eyeballs from adult zebrafish were collected per time point (ZT 1, 4,7,10,13,16,19 and 22) and the tissue stored in RNAlater (Sigma) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed three times with 100 μl PBS 1% BSA 0.1% Saponin (Sigma). Immunostaining for actin was performed by incubating with phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... washed three times and blocked again followed by incubation with rabbit anti-GAPDH antibody (Sigma; ABS16 ...
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bioRxiv - Microbiology 2021Quote: ... Wells were washed a final time and developed using the SigmaFast pNpp reagent (Sigma Aldrich). OD405nm readings were taken every minute for 15 minutes in a BioTek Synergy HT plate reader using Gen5 software ...
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bioRxiv - Microbiology 2020Quote: ... Infected cells were washed 30 min post-infection three times with warm PBS (806552, Sigma) to remove extracellular bacteria and fresh medium containing 100 μg/mL Gentamicin (15750060 ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated proteins were eluted two times with 600 μL 250ug/mL 3xFLAG peptides (SIGMA, F4799) in TBS [50mM Tris-HCl pH 7.4 ...
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bioRxiv - Plant Biology 2020Quote: ... washed 4 times with water and mounted in Eukitt (Sigma-Alrich, St-Louis, MO, USA). Images were processed using a microscope (Zeiss Axio Observer Z1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 4 times with cold RIPA buffer supplemented with Protease inhibitors (Sigma-Aldrich), resuspended in 100 μl Proteinase-K buffer with 70 μg Proteinase-K (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed three times with phosphate-buffered saline (PBS) + 0.1% Tween20 (PBS_T; Sigma-Aldrich) and blocked with ELISA diluent (5% nonfat milk [LabScientific ...
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bioRxiv - Microbiology 2022Quote: ... Cultures at time points with ratio above 1 were streaked on MacConkey’s agar (Sigma-Aldrich) containing ampicillin and kanamycin to differentiate between the two hosts.
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bioRxiv - Cell Biology 2022Quote: ... the cell number was assessed at the same time points with trypan blue solution (Sigma) and Neubauer chamber (BRAND).
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bioRxiv - Cell Biology 2022Quote: ... which were washed 5-6 times in ice-cold PBS with 1.5 mM DTT (Sigma). Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Metabolite EI fragmentation pattern and retention time were determined by ultrapure standard purchased from Sigma. Ions (m/z ...
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bioRxiv - Cell Biology 2021Quote: ... ECs were then washed three times with PBS and permeabilized with 0.5% Triton-X (Sigma) for 10 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The retention time was locked using standard mixture of fatty acid methyl esters (Sigma Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... and identified by retention time and absorbance spectra according to commercially available standard (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... pregnant females were gavaged at the specified time points with 80mg/kg tamoxifen (Sigma, #T5648) dissolved in peanut oil with 10% ethanol at a final concentration of 10mg/ml.
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bioRxiv - Physiology 2020Quote: ... Cells were washed 5 more times and incubated with 1μg/mL DAPI (Sigma-Aldrich, D9542) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The resin was washed 5 times using TBS with 0.05% IGEPAL CA-360 (Sigma-Aldrich) and PERP plus any interacting proteins were cleaved from the resin using 30 units of ProTEV enzyme (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Each antibody was washed off three times in PBS-T (PBS containing 0.05% Tween20 (Millipore)) ...
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bioRxiv - Bioengineering 2020Quote: ... the glass fiber capture membrane was washed three times in nuclease free water (Sigma-Aldrich) and dried overnight at room temperature ...