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Citations for Millipore Sigma :
1651 - 1700 of 2187 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Westerns blots were performed and normalized against b-actin (1:10,000, MABT825, Millipore, Billerica, MA, USA) as previously reported by our group [25] ...
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bioRxiv - Biophysics 2021Quote: Fluorescent cell walls were obtained by metabolic insertion of Rhodamine B (Sigma-Aldrich, St. Louis, USA). The Rhodamine dye was added to the culture medium at a concentration of 1μM ...
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bioRxiv - Genomics 2021Quote: ... 15 g/L agar) supplemented for mutant strains with hygromycin B (Sigma-Aldrich; 70 μg/ml) or nourseothricin (Werner Bioagents ...
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bioRxiv - Immunology 2021Quote: ... B Bedogni (University of Miami, FL) and were cultured in DMEM supplemented with 10% FBS (Sigma) and 1% penicillin/streptomycin (HyClone) ...
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bioRxiv - Immunology 2020Quote: ... and 0.25 μg/mL of amphotericin B per mL (complete medium - all reagents from Sigma-Aldrich). To separate the trypomastigote forms from the culture ...
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bioRxiv - Microbiology 2021Quote: ... the protein was eluted with 6 ml buffer B containing 15 mM d-desthiobiotin (Sigma Aldrich). Purification of GyrA-N-Strep and GyrB-C-Strep were conducted in the same way using an adjusted buffer Bgyr for cell disruption and purification (buffer Bgyr ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content in each well was evaluated by the SRB assay (Sulphorhodamine B, SIGMA, cat. S1402) as described elsewhere98.
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bioRxiv - Immunology 2022Quote: ... Strains expressing GFP were grown in media supplemented with 50 μg/mL hygromycin B (Sigma-Aldrich), and strains expressing luxABCDE were grown in media supplemented with 20 μg/mL Zeocin (Thermo).
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bioRxiv - Microbiology 2022Quote: Adherent neutrophils (not IL-8 treated) were exposed to 5 µg mL−1 cytochalasin B (Sigma) for 20 min followed by 1 µM fMLF (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-b-actin mAb (clone AC-15; #A1978) was obtained from Sigma-Aldrich (MO, USA). Rabbit anti-GFP pAb was from MBL (Nagoya ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates are obtained by sonication and CelLytic™ B Cell Lysis Reagent (Sigma-Aldrich, US). Cell debris are removed by centrifugation at 24,000 g and crude Rubiscos were obtained by ammonium sulfate precipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... B cell activation was achieved with by exposing cells to with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Selection was initiated after 2-3 days with 25-150μg/ml of Hygromycin B (Sigma-Aldrich) and maintained for 10-15 days ...
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bioRxiv - Synthetic Biology 2023Quote: ... Solution B is composed of 14.3 mM of each of the twenty amino acids (Sigma, USA), 236.3 mM phosphoenolpyruvate (PEP) ...
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bioRxiv - Microbiology 2023Quote: ... sclerotiorum KO and KI mutants were screened and purified on PDA supplemented with hygromycin B (Sigma) at a final concentration of 50 μg/ml.
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin/amphotericin B (Sigma, St. Louis, MO). Cells were transfected using TransIT-LT1 Transfection Reagent (Mirus Bio ...
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bioRxiv - Microbiology 2023Quote: ... 1 mg of Cyt B was dissolved into 100 μl of dimethyl sulfoxide (DMSO; Sigma-Aldrich) and 15 μl aliquots of the resulting Cyt B solution were added to each reactor ...
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bioRxiv - Cell Biology 2023Quote: ... 300 U/mL streptomycin sulphate (#A1852 AppliChem) and 7.5 µg/mL amphotericin B (#A2942 Sigma-Aldrich). Excess tissue ...
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bioRxiv - Molecular Biology 2023Quote: ... and antibiotics (100 U/ml penicillin, 100 μg/ml streptomycin, 0.25 μg/ml amphotericin B (Sigma)) at 37°C in a moist atmosphere containing 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... These flasks were treated with a 0.1% gelatin solution in water (Millipore Sigma, ES-006-B) for 30 minutes prior to plating cells ...
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bioRxiv - Immunology 2022Quote: ... CD20+IgG+ or CD20+IgD+IgM+CD27-IgG- B cells were sorted into FBS (Sigma-Aldrich) using a FACSAria Fusion cell sorter (Becton Dickinson) ...
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bioRxiv - Molecular Biology 2023Quote: ... media was changed for media containing 5,6-dichlorobenzimida-zole 1-b-D-ribofuranoside (DRB; Sigma, D1916) at a final concentration of 50 μM ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in 250 µl primary B cell media supplemented with 5 µg/ml LPS (Sigma).
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bioRxiv - Microbiology 2023Quote: ... 20 mM NaHCO3) supplemented with 10% (v/v) CelLytic™ B cell Lysis Reagent (Sigma-Aldrich) and 0.1% Protease Inhibitor Cocktail (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Two control groups were used: (a) Distilled water and (b) ivermectin (5 mg/mL, Sigma-Aldrich). A ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse monoclonal anti α-tubulin clone B-5-1-2 (Sigma-Aldrich, St. Louis, MO, USA) and anti-PABP [49] were used as loading controls ...
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bioRxiv - Biophysics 2023Quote: ... and anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma), revealed with donkey anti-mouse secondary antibodies conjugated to Alexa Fluor 555 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells expressing H2B-mRuby were then enriched using selection with 0.5 mg/ml hygromycin b (Sigma) and verified using fluorescence microscopy and flow cytometry (LSRFortessa flow cytometer ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1:10000 dilution of anti-mouse alpha-Tubulin (Clone B-5-1-2; SIGMA) for 1 to 3 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were treated with 1 μg/mL doxycycline and 50 μM biotin (Sigma-Aldrich # B-4639) for 24 hours for BirA* fusions or 30 minutes for miniTurbo fusions ...
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bioRxiv - Neuroscience 2024Quote: ... gently dounce-homogenized (KIMBLE Dounce tissue grinder, Sigma D8938, 6 strokes with A and B pestle), and incubated for 5 minutes in a total of 4 ml EZ buffer.
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bioRxiv - Neuroscience 2024Quote: ... Mass spectrometer calibration was performed with a [Glu1]-fibrinopeptide B human (Glu-Fib) (Sigma-Aldrich, USA) solution with an MS/MS spectrum reference from the NanoLock Spray source ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse embryonic stem cell medium was prepared by supplementing EmbryoMax® DMEM (Millipore, SLM-220-B) with 15% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Following amplification cells were washed for 15 min with wash buffer B (Sigma Duolink PLA kit) and incubated with Hoechst for 5 min before another 15 min wash with buffer B ...
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bioRxiv - Cell Biology 2020Quote: ... gel that was laced with 1 mg/mL of 70,000 MW Rhodamine B isothiocyanate-Dextran (Sigma-Aldrich). In this way ...
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bioRxiv - Developmental Biology 2020Quote: Embryos at 80% epiboly were dechorionated in glass dishes and treated with Lat B (428020, Sigma-Aldrich). This inhibitor of actin polymerization was applied at a dose of 0.15 μg/ml (diluted in 0.3% Danieau buffer with 1% DMSO) ...
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bioRxiv - Biophysics 2022Quote: ... encoding CPB was cloned as N-terminal His6 or N-terminal GST fusion into pET19-b (Novagen) using NcoI and BamHI sites ...
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bioRxiv - Neuroscience 2022Quote: ... rinsed with 1X PBS and embedded in 3% agarose Type I-B (Sigma-Aldrich, St. Louis, MO) dissolved in 1X PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... in ultrapure water and (B) 10 mM ammonium formate and 0.125% formic acid (Sigma, St. Louis, MO) in 95:5 v/v acetonitrile ...
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bioRxiv - Molecular Biology 2021Quote: ... and used to transduce activated B cells in the presence of 10 µg/mL polybrene (Sigma-Aldrich). Viral transduction was facilitated by centrifugation (2500 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... cells at 80% confluency were treated with 100 μM 5,6-dichlorobenzimidazole 1-b-d-ribofuranoside (DRB) (Sigma) for 3 hours ...
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bioRxiv - Biophysics 2019Quote: Glycogen phosphorylase b from rabbit muscle and Glycogen phosphorylase a from rabbit muscle were purchased from Sigma. Chemicals were purchased as follows ...
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bioRxiv - Systems Biology 2019Quote: ... Purified B cells (250,000 cells/ml) were activated with LPS (10μg/ml, Salmonella typhimurium, Sigma Catalog # L6143).
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bioRxiv - Molecular Biology 2019Quote: Mouse monoclonal α-Flag (M2) and α-tubulin (clone B-5-1-2) were obtained from Sigma. Rabbit polyclonal α-HA (ab9110 ...
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Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switchingbioRxiv - Microbiology 2021Quote: ... The transformed cells were selected on plates containing 400 mg/L Hygromycin B (Sigma-Aldrich, Espoo, Finland). Successful mating type change was determined by PCR as described below ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were pre-treated or not with 12 μM bromoenol lactone (BEL, Sigma-Aldrich) in DMEM-BSA for 30 min at 37°C and then incubated with Tf-beads (1:2 cell-bead ratio ...
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bioRxiv - Neuroscience 2020Quote: ... and finally the tip was backfilled with the K+ ionophore I-cocktail B (Fluka, Sigma-Aldrich, USA) using a 28G microfil (WPI ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was then incubated in secondary biotinylated rabbit antibody to goat antibody (1:250, Sigma, B-7024) for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... mES cells were grown under feeder-free conditions on 0.2% gelatin Type B coated dishes (Sigma Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... 100 U/ml penicillin and 100 mg/ml streptomycin and 0.01 mg/ml hygromycin B (Sigma-Aldrich). HEK293T cells were grown in DMEM3 ...