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Citations for Millipore Sigma :
1651 - 1700 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and Expand high-fidelity PCR (Sigma-Aldrich) were used to synthesize and amplify the cDNAs ...
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bioRxiv - Microbiology 2019Quote: ... PCR primers were purchased from Sigma-Aldrich and are listed in S7 Table ...
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bioRxiv - Genomics 2021Quote: ... PCR primers were obtained from Sigma-Aldrich at 100 µM concentration in H2O ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR primers were purchased from Sigma, and the Biotin-TEG DNA antisense oligos were designed in Stellaris probe designer and purchased from IDT ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and eluted with 15 μL of nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing ...
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bioRxiv - Physiology 2023Quote: ... REDExtract-N-Amp PCR ReadyMix (R4775, Sigma) was used ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using SpeedBeads (Sigma) and sequenced by Sanger sequencing.
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bioRxiv - Immunology 2023Quote: ... and the following PCR primer sequences (Sigma): Gapdh (F ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification for each gene sequence was performed with 50 ng of genomic DNA in a 20 µL reaction using a KAPA Taq PCR Kit (Sigma-Aldrich, St Louis, MO, USA) and the following cycle conditions ...
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bioRxiv - Plant Biology 2021Quote: ... 5’ RACE PCR was performed with 2 µl of 1:5 diluted cDNA in a 20 µl reaction with the KAPA2G Robust PCR Kit (KK5501, Sigma-Aldrich, St. Louis, Missouri, USA) buffer B ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from a tail biopsy and genotypes were determined using the Extract-N-Amp Tissue PCR kit (Sigma-Aldrich, St Louis, MO, USA). The following primer sequences (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The amplified DNA was then purified by depositing the PCR products in a 96-well MultiScreen PCR plate (Millipore). A vacuum of 15 inHg was applied to the wells containing the PCR products until all the wells were dry (12-15 minutes) ...
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bioRxiv - Microbiology 2020Quote: ... Detection was via polyclonal goat secondary antibodies: an ATTO647N-conjugated anti-mouse antibody was purchased from Sigma Aldrich, while an AlexaFluor488 (AF488)-conjugated anti-rabbit antibody was purchased from Invitrogen.
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bioRxiv - Developmental Biology 2021Quote: ... Detection was done with donkey anti-mouse IgG horseradish peroxidase at 1:5000 (1:5000, Millipore Cat#AP308P) and Luminata Crescendo Western HRP substrate (Millipore Cat#WBLUR0100) ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
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bioRxiv - Molecular Biology 2022Quote: ... Detection of Gpd1-TAP (p1291) was carried out by polyclonal rabbit peroxidase α-peroxidase (PAP; Sigma- Aldrich; P1291) at a dilution of 1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins of interest were visualized with the Amersham ECL Detection Reagents (GE Healthcare by SIGMA, Cat. No. RPN2105) or LiteAblot TURBO Extra-Sensitive Chemioluminescent Substrate (EuroClone ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was achieved using appropriate horseradish peroxidase-labeled secondary antibodies and Immobilon Western chemiluminescent HRP substrate (Millipore Sigma). Band intensity was determined with ImageJ 2.0.0-rc-69/1.52p software (http://imagej.nih.gov/ij ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a secondary detection antibody and immunoblots were developed with Forte HRP substrate (Millipore, Dachstein, France). Anti-Actin antibody m25 (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... Detection was performed using horseradish peroxidase conjugated secondary mouse or rabbit antibody and chemiluminescence HRP substrate (Millipore #WBKLS0100).
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... For protein detection: cell lysates were prepared in RIPA lysis buffer containing protease and phosphates inhibitors (Sigma-Aldrich) and Western blotting was performed as described (69) ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were washed and incubated in HRP-conjugated secondary antibody before detection using Immobilon ECL Ultra substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... All wells were treated with the fluorescent detection probes 2’,7’-dichlorofluorescein diacetate (10µM, DCF-DA; Sigma, D2926) or 4,5-diaminoflurescein diacetate (5µM ...
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bioRxiv - Immunology 2023Quote: ... and plates were probed with IgG1-and IgG2a-specific secondary alkaline phosphatase (AP)-conjugated detection antibodies (Sigma-Aldrich #SAB3701172 and #SAB3701179 ...
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bioRxiv - Plant Biology 2023Quote: ... The enzyme complex used for detection consisted of 0.40 mg mL-1 glucose oxidase (G2133, Sigma-Aldrich, Spain), 0.05 mg mL-1 horseradish peroxidase (77332 ...
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bioRxiv - Cell Biology 2024Quote: PLA was carried out according to the manufacturer’s protocol using Duolink In Situ Detection Reagents (Sigma Aldrich, #DUO92013) to visualize the intracellular protein-protein interactions ...
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bioRxiv - Plant Biology 2024Quote: For intracellular ROS detection we used the fluorescent dye 2ʹ,7ʹ-Dichlorofluorescin Diacetate (DCFH-DA, Sigma-Aldrich, USA). P ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Immunology 2021Quote: ... and then developed at RT by addition of 100 μL of p-nitrophenyl phosphate (pNPP) substrate (Sigma-Aldrich) per well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma, Cat N° D9663-10ML) in 0.1% PBS-Triton X-100 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were washed three times in 1x PBS before quenching with 0.1 M glycine (Roth) in 1x PBS for 10 min at RT and 0.1 % NaBH4 (Sigma) in 1x PBS for 7 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed in PBS at room temperature (RT) then permeabilized with PBS-T (0.1M PBS + 0.2% Triton-X100, Sigma) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with DAPI (diluted 1:1000 in PBS) for 3 minutes at RT and mounted with Fluoromont (Sigma).
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bioRxiv - Microbiology 2021Quote: ... followed by staining of cell nuclei for 3 min at RT with 1 μg/ml Hoechst 33342 (Sigma) in PBS−/− ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed again with blocking solution at RT and DNA was counterstained with DAPI (Sigma-Aldrich, D9564). For EdU staining ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with secondary antibodies for one hour at room temperature (RT) and mounted using Mowiol (Sigma); Hoechst (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cryo-sections were acetylated using 0,1 M triethanolamine and 0,25% acetic anhydride for 25 min at RT (respectively #90275, #A6404, Sigma). This steps are followed by a pre-hybridization using 2X of SSC ...
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bioRxiv - Bioengineering 2021Quote: ... at RT and nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL; Sigma). After brief drying ...
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bioRxiv - Bioengineering 2019Quote: ... Miller.) Reactions were carried out in an RT assay buffer containing: 60 mM Tris (77-86-1, Sigma), 30 mM KCl (7447-40-7 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 minutes each with PBST at room temperature (RT) with agitation and blocked with 5% Milk Powder (Sigma) in PBST for 1 hr at RT with agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Fish were stained overnight at room temperature (RT) with a solution of 0.1% Alcian blue 8GX (Sigma Aldrich) w/v ...
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bioRxiv - Immunology 2021Quote: ... in 3% BSA-containing DPBS for 15 min at RT and stained with phalloidin-TRITC (P1951, Sigma Aldrich) and DAPI (D9542 ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was done by extracting total RNA using Tri-reagent (SIGMA) and RT was done with Superscript II (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 30 min at RT with anti-β-actin (1:10’000, A3854 Sigma-Aldrich), which is used as a normalizer.
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation for another 1 hour at RT in a solution containing 2.5% potassium ferrocyanide (Sigma-Aldrich) in 0.1 M PIPES ...