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Citations for Millipore Sigma :
1801 - 1850 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed with TTBS and incubated for 1 h at room temperature with isotype specific secondary antibodies anti-mouse IgG (1:10,000; Sigma-Aldrich, A4416) and anti-rabbit IgG (1:80,000 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were treated for 2 h at room temperature with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA), and 0.3% PBS-T as a blocking agent ...
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bioRxiv - Cell Biology 2023Quote: ... Each lysate was subjected to immunoprecipitation for 2 h or overnight with anti-FLAG M2 affinity agarose affinity gels (Sigma-Aldrich) or anti-HA magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampal slices were post-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 24 h and transferred to 30% sucrose (Sigma-Aldrich). Slices were sectioned coronally at 20 μm and stored at −20 °C in cryoprotectant (20% glycerol ...
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bioRxiv - Developmental Biology 2023Quote: ... #3 and #4 were pooled and processed to obtain a single cell suspension through mechanical disaggregation followed by 2 h of enzymatic digestion in DMEM + 125 μg/mL collagenase (Sigma-Aldrich), 0.25 µg/mL dispase (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 10 dpi and ending at 34 dpi.
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bioRxiv - Immunology 2023Quote: ... cells were restimulated after 72 h of culture with PMA (50 ng ml-1) and ionomycin (1 µg ml-1, both from Sigma-Aldrich) in the presence of brefeldin A (5 µg ml-1 ...
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bioRxiv - Cell Biology 2023Quote: ... A fraction of the cell lysates was used to estimate NAD(H) levels using the NAD/NADH quantification kit (Sigma MAK037) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... the aortic tissue was incubated for at least 12 h in Roti®Histol (#6640.4, Carl Roth) at RT and embedded in paraffin (#P3558, Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Host embryos were prepared by thawing frozen embryos and incubating them for 24 h in an M16 medium (M7292, Sigma-Aldrich). Further ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE (90 V, 30 min; 120 V, 1 h) and transferred to a Polyvinylidene Fluoride (PVDF, Millipore, IPVH00010) membrane (290 mA ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Stationary phase cultures (37°C, 18-24 h, with shaking) were washed twice with sterile phosphate buffered saline (PBS; D8537, Sigma-Aldrich) and diluted to 5 x 105 CFU.mL−1 for experiments ...
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bioRxiv - Biochemistry 2023Quote: ... 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of TEV protease (10 U/µL) (T4455, Sigma-Aldrich) in SEC buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were adjusted to the same concentration and incubated for 2 h overhead shaking with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). Beads were washed three times for5 min with mild wash buffer (20 mM HEPES-KOH (pH 7.9) ...
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bioRxiv - Cancer Biology 2024Quote: ... Knockdown of ATF4 was verified by western blot analysis following 72 h of treatment with 1 µg/mL doxycycline (#D9891, Sigma-Aldrich). Sequences are provided in Supplementary Table 1.
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bioRxiv - Neuroscience 2024Quote: ... After 5 min of washing in PBS the skin sections were permeabilized and blocked for 1 h in a 10 % Donkey serum (Sigma Aldrich) and 0.3% Triton-X 100 (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Tetramer staining was performed in combination with staining for chemokine receptors CXCR5 and CXCR6 for 1 h at RT in staining buffer supplemented with Dasatinib (50 nM) and biotin (20 nM; Sigma, B4501). After washing of the samples with FACS buffer (PBS supplemented with 1 % FCS) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... Viral transduction was performed by spinfection at 900 g at 25 °C for 3 h with 10 μg/ml polybrene (Sigma-Aldrich), followed by resting for 3 h at 37 °C in 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... Flow-troughs were left to incubate for 17 h at 37 °C before the tryptic digestions were terminated with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich) at a final concentration of 0.6% ...
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bioRxiv - Cell Biology 2024Quote: The samples were fixed in 4% PFA for 1 h at room temperature (RT) and then incubated with 15% sucrose solution (Sigma Aldrich) supplemented with 0.03% eosin (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... virus supernatants (30 ml) were treated for 1 h at 37°C with 2500 U ultrapure benzonase nuclease (Sigma, E8263-25KU) and subtilisin64 (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Polarization of M0 macrophages for 48 h in macrophage medium supplemented with 100 ng/mL lipopolysaccharide (LPS; Escherichia coli O127:B8, Sigma Aldrich) and 20 ng/mL interferon-γ (Peprotech ...
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bioRxiv - Developmental Biology 2024Quote: ... Retinas were dissected and kept 24 h in culture medium (DMEM-F12 without phenol red, supplemented with 1 mM glutamine (Sigma Aldrich), 1% penicillin/streptomycin (Sigma Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... and for reconstitution experiments were transduced for 48 h with the respective lentiviral particles (see below) in the presence of polybrene (8 μg/ml, Sigma-Aldrich) prior to selection with puromycin (1 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... we probed the primary antibodies using Duolink PLA probes rabbit PLUS and mouse MINUS at 37 °C for 1 h and then used Duolink In Situ Detection Reagents Red (Sigma Aldrich) to detect the proximity between the proteins of interest ...
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bioRxiv - Plant Biology 2024Quote: ... nitrocellulose membranes were incubated at RT for 2 h with anti-GFP (Miltenyi 130-091-833) or anti-Flag (Sigma A8592) antibodies conjugated with horseradish peroxidase in TBS-T at 1:5000 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase conjugated secondary antibodies (table 2) were added for 1 h at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
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bioRxiv - Cancer Biology 2024Quote: ... domes were overlaid with basal organoid media containing 5000 primary fibroblasts from Lkb1fl/fl mice previously adenotransducted with AdCre/AdGFP and incubated for 24 h with or without 1ug/ml LPS (Sigma L4516).
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bioRxiv - Neuroscience 2024Quote: ... followed by the corresponding Alexa Fluor secondary antibodies with Hoechst nucleus counterstaining (bisBenzimide H 33342 trihydrochloride, Sigma, B2261, 1 μg/ml) at RT for 2 hrs ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the PG sample was digested for 3 h at 37°C with 4 µg of lysozyme (Sigma-Aldrich) and then ...
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bioRxiv - Microbiology 2024Quote: ... the Fab portion of antibody CP33-H/L-LA22 was covalently coupled to a cyanogen bromide-activated Sepharose® 4B resin (Sigma) at a concentration of 10 mg Fab per mL of resin ...
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bioRxiv - Microbiology 2024Quote: ... The secondary antibody incubation was carried out for 1 h at room temperature before the cells were washed and counterstained with DAPI (Sigma Aldrich). The plates were imaged using the Operetta CLS confocal microscope (PerkinElmer) ...
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bioRxiv - Microbiology 2024Quote: ... cells were treated with DMSO only or preincubated for 1 h with CHX (10 µg/ml, Sigma-Aldrich, Cat. No.: C6255) plus Nec-1s (10 µM) ...
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bioRxiv - Microbiology 2024Quote: ... 1 mg of protein lysates were incubated for 16-18 h at 4 °C with 25 μl of M2 anti-FLAG resin (Sigma Aldrich). The captured complexes were washed four times using 20 mM Tris–HCl pH 7.9 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at RT and incubated for ∼16 h at 4°C with anti-Ly6H and anti-MAP2 diluted in Duolink Antibody Solution (Millipore Sigma). The slices were washed four times for 5 min with PBS and incubated for 30 min at RT with donkey anti-mouse conjugated to Cy3 and donkey anti-chicken conjugated to Alexa Fluor 647 in 10% normal donkey serum in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... 2% H2O (MilliQ) and were shaken for 3 h before precipitation with 45 mL of ice cold 1:1 diethyl ether (Sigma-Aldrich) and hexanes (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... The sample was pipetted onto a gel subbed microscope slide and allowed to dry 24 h before staining with Shorr Stain (Sigma Aldrich). Gel subbing was performed in house using standard IHC protocol to coat glass slides in gelatin/CrK(SO4)2 solution99 ...
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bioRxiv - Microbiology 2024Quote: ... the blots were incubated at room temperature on a rocker for 1 h with secondary anti-mouse IgG-HRP (Sigma; A4416) or anti-rat IgG-HRP (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... for 1 h at 57°C and alkylated for 1h in the dark with 55 mM iodoacetamide in 100 mM NH4HCO3 (Sigma Aldrich), washed in 25 mM NH4HCO3 ...