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Citations for Millipore Sigma :
1851 - 1900 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were adjusted to the same concentration and incubated for 2 h overhead shaking with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). Beads were washed three times for5 min with mild wash buffer (20 mM HEPES-KOH (pH 7.9) ...
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bioRxiv - Neuroscience 2024Quote: ... After 5 min of washing in PBS the skin sections were permeabilized and blocked for 1 h in a 10 % Donkey serum (Sigma Aldrich) and 0.3% Triton-X 100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 24 h at 4°C with a mixture of a monoclonal mouse anti-chondroitin sulfate antibody (CS-56, 1:500; Sigma-Aldrich) and a polyclonal rabbit anti-PV antibody (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: The samples were fixed in 4% PFA for 1 h at room temperature (RT) and then incubated with 15% sucrose solution (Sigma Aldrich) supplemented with 0.03% eosin (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... virus supernatants (30 ml) were treated for 1 h at 37°C with 2500 U ultrapure benzonase nuclease (Sigma, E8263-25KU) and subtilisin64 (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Flow-troughs were left to incubate for 17 h at 37 °C before the tryptic digestions were terminated with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich) at a final concentration of 0.6% ...
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bioRxiv - Immunology 2024Quote: ... Viral transduction was performed by spinfection at 900 g at 25 °C for 3 h with 10 μg/ml polybrene (Sigma-Aldrich), followed by resting for 3 h at 37 °C in 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Homogenate was subjected to high speed centrifugation (100 000g, 1 h, 4 °C) and supernatant was neutralized with 0.5M Tris-HCl (pH 6.8) (Sigma, St.Louis, MO, USA). Aβ1-40 levels in brain and plasma samples were quantified by ELISA using Meso Scale Discovery (MSD ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at RT and incubated for ∼16 h at 4°C with anti-Ly6H and anti-MAP2 diluted in Duolink Antibody Solution (Millipore Sigma). The slices were washed four times for 5 min with PBS and incubated for 30 min at RT with donkey anti-mouse conjugated to Cy3 and donkey anti-chicken conjugated to Alexa Fluor 647 in 10% normal donkey serum in PBS ...
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bioRxiv - Physiology 2024Quote: ... cells were loaded with 10 nM tetramethyl rhodamine methyl ester (TMRM) supplemented with Cyclosporin H (2 mg/ml; Sigma Aldrich, SML1575) to inhibit multidrug- resistance pumps ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were followed by 12 h culture in the presence of microtubule-disrupting agent nocodazole at 100 µM concentration (Sigma-Aldrich). To remove nocodazole ...
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bioRxiv - Biophysics 2024Quote: ... Wild-type 3T3 cells at 60-80% confluency were incubated for 24 h with 100X virus suspension and 8 µg/ml of Polybrene Transfection Reagent (Millipore Sigma). To maintain stable knockdown ...
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bioRxiv - Cancer Biology 2024Quote: ... washed 3 x 10 min in PBS and incubated for 1 h in PBS containing 10 mg/mL sodium borohydride (Sigma, 213462) to quench autofluorescence ...
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bioRxiv - Microbiology 2024Quote: ... the blots were incubated at room temperature on a rocker for 1 h with secondary anti-mouse IgG-HRP (Sigma; A4416) or anti-rat IgG-HRP (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The sample was pipetted onto a gel subbed microscope slide and allowed to dry 24 h before staining with Shorr Stain (Sigma Aldrich). Gel subbing was performed in house using standard IHC protocol to coat glass slides in gelatin/CrK(SO4)2 solution99 ...
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bioRxiv - Immunology 2024Quote: ... IgGs were incubated for 4–5 h at 37°C in digestion buffer (4% (w/w of IgG) activated Papain from papaya latex (Sigma Aldrich), 10 x L-Cysteine ...
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bioRxiv - Neuroscience 2024Quote: The mounted sections for Nissl and H&E were dried overnight at 33°C in the dry oven and then stained for Nissl substance using 0.2%Thionine (SRL Chemicals, India) and H&E (Sigma Alrdrich, India) using the Harris’ regressive protocol ...
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bioRxiv - Microbiology 2024Quote: ... Blots were then blocked in 8% (w/v) semi-skimmed milk powder in PBS for at least 2 h at RT (Sigma Aldrich) before adding 15 µg anti-Aur IgY antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... dCas9-BE-expressing clones were seeded in 6-well plates and grown until approximately 30% confluency (24–48 h) then treated with 8 µg/mL polybrene (Sigma, #107689) for 2–4 h ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with 10 μg mL-1 Alexa Fluor Plus 647- conjugated donkey anti-mouse IgG [H+L] and 0.2 μg mL-1 DAPI (Sigma-Aldrich D9542) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A iCas9 p53KO cells were transfected with siRNA and after 66 h treated with 150 μM DRB (5,6-dichloro-1- beta-D-ribofuranosylben- zimidazole, Sigma, D1916-10MG) for 6 h ...
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bioRxiv - Cell Biology 2024Quote: ... 1:50,000 dilution in TBS-T) for 1 h at room temperature (Anti-rabbit IgG peroxidase antibody produced in goat, A0545, Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral supernatants collected 24h post-transfection was used to infect subconfluent K19-KO cells in three sequential 4 h incubations in the presence of 4 ug/ml polybrene (Sigma-Aldrich). Transductants were selected using a culture medium containing 100 ug/ml hygromycin beginning 48h after infection ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were incubated at 37 °C and 5% CO2 for 72 h in a humidity chamber until resazurin (#R17017, Sigma Aldrich) dissolved in PBS was added to a final concentration of 0.01 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were blocked with 5% (wt/vol) nonfat milk in PBST for 1 h at room temperature and probed with primary antibodies against Flag (Sigma, F1804), CST 12364S ...
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bioRxiv - Cell Biology 2024Quote: ... Viral supernatants were filtered and used to infect cells by spin-infection (2500 rpm centrifugation at 25 °C for 1 h) in the presence of 4 µg/mL polybrene (Cat#S2667, Sigma-Aldrich). Transduced cells were enriched either by antibiotic selection or expression of fluorescent protein using a BD FACSAria Fusion cell sorter.
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bioRxiv - Developmental Biology 2024Quote: ... bovine serum albumin) and incubated for a further 48 h in 1:100 fluorescein isothiocyanate (FITC)-labelled anti-rabbit secondary antiserum (Sigma Aldrich). A final overnight incubation in 1:100 TRITC-labelled phalloidin (200 ng/ml ...
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bioRxiv - Biophysics 2020Quote: ... flat 1:30 PDMS stamps were incubated for 1 h with 40 μl of 50 μg/ml Alexa Fluor 647 labelled and 50 μg/ml unlabelled fibronectin (Sigma Aldrich, F1141), then washed and dried ...
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bioRxiv - Cell Biology 2020Quote: ... confluent cells were serum starved for 24 h in the presence of 50 μg/ul L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902). At the time of assay ...
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bioRxiv - Cell Biology 2020Quote: ... the oocytes were blocked in washing buffer with 3% BSA for 1 h at room temperature and then incubated overnight at 4°C with FITC-labelled mouse anti-α-tubulin (1:200, Sigma-Aldrich) or mouse monoclonal anti-γ-H2AX antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were washed 3 times with TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in 1 % BSA solution in TBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclei were lysed for 1 h at 4 °C in a presence of 150 U of Benzonase nuclease (Novagen, cat. no. 71206) while rotating ...
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bioRxiv - Cell Biology 2022Quote: ... the patterned surface was incubated for 1 h with a mixture of 50 μg/mL fibronectin (Sigma-Aldrich, St. Louis, MO, USA), 5 μg/mL concanavalin A (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... The supernatant containing viral particles was harvested at 30 h and passed through a 0.45 µm PVDF Millex-HV Syringe Filter (Millipore cat. no. SLHV033RS) to remove cells and debris ...
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bioRxiv - Neuroscience 2021Quote: The other two remaining free-floating brain sections were washed in 0.1% PBS with 0.1 % Triton A-10 (PBS-T) and incubated in blocking solution (1 h at room temperature; PBS-T with 10% donkey serum, Sigma Life Science). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of AsCas12a nuclease in the stable HEK 293 T-REx cell line was induced by 100 ng/mL tetracycline for 24 h and assayed by Western immunoblotting using FLAG-tag specific antibodies (Sigma-Aldrich, F1804).
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bioRxiv - Cell Biology 2020Quote: ... The following day cells were treated for 8 h with either ES DMEM-2i + DMSO or ES DMEM-2i + CHX (100 ug/ml (Sigma-Aldrich, 01810)) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed at 4°C for 3 h using 5 µg of an anti-FLAG M2 antibody (Sigma-Aldrich, F1804, mouse). For RIP-Seq ...
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bioRxiv - Immunology 2021Quote: PBMCs were isolated from buffy coats and allowed to adhere to tissue culture treated flasks for 2 h in Promocell monocyte attachment buffer (Sigma, Dorset, UK). Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-M4 polyclonal antibody (1:200; H-175, Millipore, Burlington, USA) in PBS containing 0.1% Triton X-100 and 1% normal donkey serum for 2 days at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... the following secondary antibodies (1:100) were applied for 2 h at room temperature on a rotating platform: Atto 594 (anti-mouse, Sigma-Aldrich, 76085) and Abberior STAR 635 P (anti-rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 1 h incubation at RT in secondary antibodies (Anti-Rabbit: A0545; Anti-Mouse: A6782, Sigma-Aldrich Quimica S.A., Madrid, Spain) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the gels were incubated for 1 h on a shaker at room temperature with either with 20 μg/mL Atto 594 NHS ester (Sigma-Aldrich, 08471) or 20 μg/mL Atto 647N maleimide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed thrice in PBS and incubated on ice with 100 μL of either 10 μg/mL Anti-Factor H Goat pAb (Sigma, 341276-1ML) or 1 μg/mL Goat anti Chicken IgY (H+L ...
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Plant Biology 2020Quote: Seeds were surface sterilized and imbibed in sterile water in the dark for 72 h before sowing on MS basal medium (Sigma Life Science). After 10 days ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from Legionella strains cultured for 18 h in AYE medium using the GenElute™ Bacterial Genomic DNA Kit (NA2100, Sigma-Aldrich) and used as template for PCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... A 1% inoculum of an overnight culture of the transformed strain was transferred to 20 mL pre-reduced BHIs broth and incubated for 4 h before being induced with anhydrotetracycline (Sigma Aldrich, USA) to a final concentration of 500 ng/mL in a 20 mL culture for 1h under anaerobic conditions ...
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bioRxiv - Microbiology 2020Quote: ... at 30°C for 24-48 h then stored at 4°C prior to propagation in yeast peptone dextrose (YPD; Sigma-Aldrich, UK) medium overnight (16 h ...