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Citations for Millipore Sigma :
2051 - 2100 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 10% FBS/PBS for 1 h and incubated with the anti-G-quadruplex antibody (BG4, Sigma-Aldrich, 1:200) overnight at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Biochemistry 2023Quote: ... Protein pellets were resuspended in 50 µL of 50 mM NH4HCO3 and digested for 16 h with trypsin (Sigma-Aldrich, Product code T6567) with an enzyme to protein ratio of 1:20 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416, RRID:AB_258167; anti-rabbit, Sigma-Aldrich Cat# A6154, RRID:AB_258284) (1/2000) ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743; anti-pan-cytokeratin, Millipore Sigma, C5992 ...
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bioRxiv - Microbiology 2024Quote: ... this culture was incubated for a further 1 h before washing three times and RNA was extracted using Triazole (Sigma Aldrich, England, UK) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... DNA present in the homogenate was digested at 37°C for 1 h with 125 units benzonase (Sigma-Aldrich, St. Louis, MO, USA) before 70 µg of proteins were used for standard Western blotting ...
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bioRxiv - Cell Biology 2024Quote: Paraffin sections from the mid-regions of the Swiss rolls underwent Hematoxylin & Eosin (H&E) staining (Harris Hematoxylin, Electron Microscopy Science, 26754-01; Eosin, Sigma HT110116) for standard pathology evaluation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were digested at 24 °C for 1 h in EMMS-Ca2+ supplemented with 5 mg/mL lysing enzyme (Sigma-Aldrich, L3768-1G), 3 mg/mL zymolase (nacalai tesque ...
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bioRxiv - Microbiology 2020Quote: ... Following a 15 min entry phase under standard cell culture conditions the cells were fixed and the nuclei stained for 1 h at RT by addition of 33 μl 16% PFA and 4 μg / ml Hoechst 33342 (Sigma-Aldrich, St. Louis, USA) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... each intact brain was rinsed three times (6 h for two washes and 12 h for the third wash) at 4°C in a 0.01 M PBS solution (Sigma-Aldrich Inc., St. Louis, US). Then the brain was subsequently dehydrated via immersion in a graded series of ethanol mixtures (50% ...
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bioRxiv - Neuroscience 2020Quote: ... each intact brain was rinsed three times (6 h for two washes and 12 h for the third wash) at 4°C in a 0.01 M PBS solution (Sigma-Aldrich Inc., St. Louis, US). Then the brain was subsequently dehydrated via immersion in a graded series of ethanol mixtures (50% ...
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bioRxiv - Biochemistry 2021Quote: ... and lymph node from BALB/cJ mice and humans were harvested and either fixed for 48 h at RT in 10% neutral buffered formalin (Sigma-Aldrich, St. Louis, MO) or zinc-fixation buffer (pH 6.5 - 7) ...
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bioRxiv - Cell Biology 2020Quote: ... for wild type or mutant BAG3-GFP and subjected to 6 h dynein inhibition with Dynarrestin (25μM, SML2332-5MG, Sigma-Aldrich, Saint Louis, MI, USA) or erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Viruses were harvested after 72 h of transfection and were used to infect 1×106 MCF10A together with polybrene (Sigma-Aldrich, Cat. No. S2667) reagent (4 µg/ml) ...
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bioRxiv - Immunology 2022Quote: ... IL-10 and TNF-α were determined in 24 h culture supernatant of UNT control and treated MOs using respective ELISA kits (Sigma-Aldrich kit, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO). They were then stimulated with peptide pools for 12 h ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were further incubated at room temperature for 1 h with a blocking buffer (3% normal donkey serum; S30, Chemicon by EMD Millipore, Billerica, MA, USA) in PBS solution containing 0.2% Triton X-100 and 0.05% Tween 20 (PBST) ...
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bioRxiv - Neuroscience 2021Quote: ... Subjects were recorded for 24-h baseline in their home cage before administration of 80 mg/kg pentylenetetrazole (Sigma Aldrich, St. Louis, MO, USA) injected intraperitoneally ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... the cells were incubated for 1 h in the dark and at RT with 30 μL drops containing secondary antibody and DAPI (Sigma, 10 236 276 001) diluted 1:1000 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the coverglasses were coated for 2 h at room temperature (RT) with a 20 μg/ml solution of poly-D-lysine (PDL; Sigma–Aldrich, cat. no. P6407) in sterile double distilled water (ddH2O) ...
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bioRxiv - Immunology 2022Quote: ... The specimens were incubated with antibodies overnight then washed with 0.3% Triton X-100 in PBS and stained against actin by incubation for 1 h with Phalloidin-Atto425 (Sigma-Aldrich, 66939, dilution 1:50). If indicated ...
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bioRxiv - Cell Biology 2021Quote: ... and injected into mice 48 and 76 h after DOX-chow addition to establish the effect of ML204 and AC1903 (both from Sigma-Aldrich, St. Louis, MO) on ACR ...
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bioRxiv - Pathology 2021Quote: ... Paraffin-embedded 5-μm thick sections mounted on glass slides were stained with hematoxylin and eosin (H&E) and thioflavin S (Sigma-Aldrich, St. Louis, MO). Braak neurofibrillary tangle stage (NFT) ...
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bioRxiv - Cell Biology 2020Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells (KO2) in three sequential 4 h incubation in the presence of 4 µg/ml polybrene (Sigma-Aldrich, St Louis, MO). Transductants were selected in hygromycin (100 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... cells were transduced with 2 ml lentivirus for 24 h in the presence of 12 μg/ml of polybrene (Sigma-Aldrich, St. Louis, MO). At 24 h post-transduction ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were further incubated in medium containing 300 nM tamoxifen for 48 h and then incubated with two-fold dilutions of AdV-C5-EGFP stock virus in DMEM medium (Sigma-Aldrich, cat. no. D6429) supplemented with 7.5% fetal calf serum (Gibco/ Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... reduced and alkylated according to the manufacturers protocol and digested overnight with trypsin (SERVA, Heidelberg, Germany) followed by 4 h of digestions with chymotrypsin (Sigma-Aldrich, St. Louis, USA) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Subsequently, samples were incubated for 12 h in a mixture of Cryomatrix (Epredia, Catalog No. 6769006) and 30% sucrose (Sigma-Aldrich, Catalog No. 84097) (mixing ratio 50/50) ...
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bioRxiv - Neuroscience 2024Quote: 0–1 h old larvae were fed yeast paste (sham-controls) or yeast paste containing 100 mg/mL Hydroxy Urea (HU, Sigma Aldrich, St Louis, MO) for 4 h via standard protocol (41 ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 3% bovine serum albumin in 0.5% Tween-20/Tris-buffered saline (TTBS) for 1 h and incubated with an anti-2,4-dinitrophenylhydrazone (DNP) adducts polyclonal antibody (1:100, EMD Millipore, Billerica, MA, USA, #MAB2223) or HNE polyclonal antibody (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... and culture supernatants were harvested after 24 h for analysis of cytokines (CXCL1 and IL-6) and MMP2 activity (Luminex bead array, Millipore Sigma, St. Louis, MO).
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bioRxiv - Immunology 2024Quote: Biotinylated monomers were diluted to a concentration of 0.5 mg/mL with 0.1 M phosphate buffer and incubated with 0.4 mg/ml of at 37°C for 72 h in the presence of 2.5 mg/ml n-Octyl β-D- glucopyranoside (OG) and 1 mM Pefabloc SC (Sigma–Aldrich, St. Louis, MO). Peptide loaded monomers were subsequently conjugated into tetramers using R-PE streptavidin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Immunology 2024Quote: Cells from draining popliteal lymph nodes (1 x 106) were stimulated for 4 h at 37 °C with PMA (phorbol 12-myristate 13-acetate, 10 ng/mL, Sigma-Aldrich, Darmstadt, He, Germany) and Ionomycin (10 ng/mL ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed thrice using PBS and blocked and permeabilized using saponin-BSA buffer for 1 h at RT (0.2% saponin from Millipore and 1% BSA from Fisher). Cells were incubated with the following primary antibodies for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: The cells were then incubated at 37°C for 4 h in the presence of 25 μM 5-iodo-2′-deoxyuridine (IdU; Sigma-Aldrich, St. Louis, MO). After washing cells with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS 1X and incubated during 1h at room temperature with Alexa FluorTM 594 donkey anti-rabbit IgG (H+L) (Sigma-Aldrich, cat. nr A21207) secondary antibody 1:500 was used ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 min at 95 °C, and then mixed with 50 μL hybridization buffer H (4x SSC, 4 mg/ml acetylated BSA (Sigma Fluka Aldrich, B8894-1.5mL), 20 mM vanadyl ribonuclease complex) ...
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bioRxiv - Physiology 2024Quote: ... Tissues/organs were then permeabilized by incubating on a rocker for 1 h at RT in 4% Triton X-100 (Sigma Aldrich, Oakville, ON, Canada), 10% normal sheep serum (NSS ...
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bioRxiv - Biochemistry 2024Quote: ... cell pellets were collected and lysed at 4 °C for 1 h in 1x PBS+1% Tritonx-100+1x Complete protease inhibitor (Sigma-Aldrich, cat. no. 11697498001), and centrifuged at > 20,000x g at 4 °C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: Cell pellets were collected and lysed at 4 °C for 1 h in 1x PBS+1% Tritonx-100+1x Complete protease inhibitor (Sigma-Aldrich, cat. no. 11697498001). The lysates were then centrifuged at 12,000x g at 4 °C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... tissue culture plates or acid-washed and sterilized glass coverslips were coated with PLL (Sigma Aldrich, # P4707, 100 μg/ml for 1.75-2 h). The dorsal 1/5th of the spinal cord of E13 rat neural tubes were micro-dissected and quickly washed once in cold Ca2+/Mg2+-free HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... and cleavage was allowed for 2 h at room temperature before it was stopped by the addition of 2 mM benzamidine hydrochloride (Sigma, cat. no. 434760-5G). The solution was concentrated to 600 μl and β-arrestin was isolated on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: Cryopreserved peripheral blood mononuclear cells were thawed and rested for 6 h in R10 supplemented with 50 U/mL Benzonase Nuclease (Sigma-Aldrich, St. Louis, MO) at 37°C followed by stimulation with peptide pools for 12 h ...
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bioRxiv - Genetics 2022Quote: ... 2% normal goat or donkey serum and 0.1% Triton X-100 in PBS (-) at room temperature for 1 h and reacted with mouse anti-parvalbumin monoclonal antibody (Sigma-Aldrich, P3088, 1:4000), goat anti-somatostatin antibody (Santa-Cruz ...