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Citations for Millipore Sigma :
1701 - 1750 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Primary hippocampal neurons were plated at density of 120 cells/mm2 on 3.5-cm diameter Petri dishes (Falcon® 35 mm, 353001) treated for 24 h with poly-L-lysine (0.1 mg/ml; Sigma-Aldrich) in borate buffer (0.1 M) ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... MEK, Akt1, Akt2, or Akt3 for 72 h, or treated with FTY720 phosphate (FTY720-P, Cayman: 10008639) or triciribine (Sigma, T3830) as indicated ...
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bioRxiv - Neuroscience 2021Quote: SH-SY5Y and HEK293 cells were treated with SB202190 (20 μM) for 3 h in the presence or absence of 100 nM Mito-TEMPO (Sigma-Aldrich) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... 24 h post transfection cells were lysed in 300 uL of RIPA buffer containing protease inhibitor cocktail (cOmplete™ Mini, Sigma) for 30 min at 4°C with occasional vortexing ...
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bioRxiv - Immunology 2021Quote: ... Cultured BMDMs were stimulated with heat-killed KP (MOI = 10) up to 8 h or Klebsiella LPS (1 ug/ml) (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Supernatants containing the nanoparticle were harvested 72 h after transfection and concentrated with Labscale TFF system equipped with 100 kDa and 500 kDa MWCO filters (Millipore Sigma). The concentrates were purified by size exclusion chromatography (NGC Medium-Pressure Liquid Chromatography ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... followed by co-incubation with an excess of HeLa cells (recipient) for 3 h in the presence of 50 μg/ml cycloheximide (Sigma, # C104450), to prevent further protein synthesis in the heterokaryons ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 transformed with recombinant pGEX-2T plasmids were diluted 1:10 with fresh medium and grown for 1 h at 37°C before inducing with 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma). After another 4 h incubation at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked in %5 BSA for 2 h at room temperature and incubated with anti-desmin antibody (1:20; Sigma, D8281) for two overnights ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was precleared with 25 µl of beads for 1.5 h and 10 µg of chromatin were incubated overnight with each antibody: 10 µl Anti-MAFB antibody HPA005653 (Sigma-Aldrich) and 5 µl Anti-GR antibody C15200010-50 (Diagenode) ...
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bioRxiv - Cell Biology 2021Quote: ... XL-413) for 1 h followed by inhibitor incubation together with 100 μM 5-chloro-2ʹ-deoxyuridine (CldU; Sigma-Aldrich, Germany) and ...
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bioRxiv - Genomics 2022Quote: ... and 40 h later treated with 2 μg/mL doxycycline hyclate or seeded in 96-well plates at 3e3 cells per well and 24 h later treated with 1 μg/mL doxycycline hyclate and either DMSO or 10 μM MG132 (Sigma-Aldrich). Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... at 50 μM and 100 μM concentrations while H+-dependent motility was inhibited using the protonophore Carbonyl cyanide 3-chlorophenylhydrazone (CCCP, C2759 Sigma-Aldritch).
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg dsRNA were added and incubated for 1 h at 27°C, before adding 1 ml full medium (ExpressFive SFM Invitrogen, 16 mM Glutamine, 10% FBS (Sigma-Aldrich), 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were incubated for 1 h with 1 μg/ml DAPI and 50 pmol Atto 550-conjugated Phalloidin (19083, Sigma-Aldrich). Samples were washed and imaged in PBS images were acquired with a confocal laser scanning microscope (Leica TCS SP8X ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes, Sigma-Aldrich). The PLA probes that bind to the constant regions of the primary antibodies contain a unique DNA strand ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 2 mM thymidine for 24 h followed by release from thymidine into fresh medium containing 100 ng/ml nocodazole (Sigma, M1404) for 18 h ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... for 1 h and for inhibition of stress granule formation cells were treated with 1.5 µg/ml of ISRIB (Sigma-Aldrich, SML0843). Stable HeLa cell lines constitutively expressing human marker proteins for membraneless compartments ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... heparin synchronised (4 h invasion window) trophozoite stage parasites were treated for one cell cycle (trophozoite to trophozoite) with GlcN (Sigma-Aldrich) and parasite pellet extensively washed in PBS containing protease inhibitor cocktail (PI ...
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bioRxiv - Molecular Biology 2022Quote: ... hCAI with the K978C mutation (h^K978C) or for T2A mutagenesis were designed using SnapGene software (SI, Table 7) and synthesised by Sigma Aldrich.
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bioRxiv - Systems Biology 2022Quote: ... The tip of the silanized pipettes was filled with a H+-selective ionophore cocktail (hydrogen ionophore I cocktail A, Sigma-Aldrich) by dipping the tip of the pipette into the solution ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates prepared from the cells at 24 h post transfection were incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich) or HaloLink resin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Developmental Biology 2024Quote: Fully grown oocytes were harvested from female mice at 28–35 days of age that were superovulated 44 h later by injection of 5 IU of PMSG in 37 °C pre-warmed M2 medium (M7167; Sigma-Aldrich) and cultured in M16 medium (M7292 ...
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bioRxiv - Developmental Biology 2024Quote: Total mRNA was extracted from larval brains of control (yw) and mrtf[Delta7] at 24 h ALH using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media from confluent 7PA2 cells or control CHO cells cultured in plain DMEM for 16 h was concentrated 10x by using Amicon Ultra Centrifugal Filter with 3kDa cutoff (Millipore UFC500324). The concentration of Aβ1-42 was determined by ELISA assay (Thermo Fisher Scientific KHB3442) ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20 x 107 HeLaK cells pre- synchronized by thymidine treatment (2 mM for 18 h) were released to undergo S-phase in presence of F-ara-Edu (5 µM; Sigma-Aldrich) for 5 h ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was cleared by centrifugation (158,000 g, 4°C, 1 h) and incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220) for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... then were incubated with the secondary antibody (Alexa-Fluor®-647-conjugated goat anti-mouse IgG H+L,1:2000 dilution) for 60 min at room temperature and counterstained with DAPI (Sigma-Aldrich) for another 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mg/mL of streptomycin (P/S) (Cat# 4-01F00-H, BioConcept) and 10 % fetal bovine serum (FBS) (Cat# S0615, Sigma-Aldrich) at 37 °C in a humidified atmosphere with 5 % CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-L-Lysine and Laminin coating of 24-well plates was performed by 48 h incubation at 37°C using Tissue Culture water (Sigma, W3500) supplemented with 1 μg/ml Poly-L-Lysine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stimulated for 4 h with one of the following: LPS (E. coli 0127:B8 100 ng/ml; Sigma-Aldrich); solutions of mix of synthetic neuromelanins (NM 10 μg/mL of Pheo-β-LG and 10 μg/mL of Pheo-β-fl) ...