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Citations for Millipore Sigma :
1751 - 1800 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... After incubation of primary antibodies at 4°C overnight and secondary antibodies for 1 h at RT (Alexa Flour 488 and 594) cells were embedded in Mowiol (Sigma-Aldrich) and subjected to imaging with the software Axiovision 4.8 at the microscope Axio Observer Z.1.
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates prepared from the cells at 24 h post transfection were incubated with anti-FLAG M2 magnetic beads (Sigma-Aldrich) or HaloLink resin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Biochemistry 2024Quote: ... The lysate was cleared by centrifugation (158,000 g, 4°C, 1 h) and incubated with anti-FLAG M2 affinity gel (Sigma-Aldrich, A2220) for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... then were incubated with the secondary antibody (Alexa-Fluor®-647-conjugated goat anti-mouse IgG H+L,1:2000 dilution) for 60 min at room temperature and counterstained with DAPI (Sigma-Aldrich) for another 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... Conditioned media from confluent 7PA2 cells or control CHO cells cultured in plain DMEM for 16 h was concentrated 10x by using Amicon Ultra Centrifugal Filter with 3kDa cutoff (Millipore UFC500324). The concentration of Aβ1-42 was determined by ELISA assay (Thermo Fisher Scientific KHB3442) ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20 x 107 HeLaK cells pre- synchronized by thymidine treatment (2 mM for 18 h) were released to undergo S-phase in presence of F-ara-Edu (5 µM; Sigma-Aldrich) for 5 h ...
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bioRxiv - Immunology 2024Quote: ... gut permeability was evaluated by gavaging mice that were fasted for 4 h with 5 mg FITC-dextran 4000 (Sigma -Aldrich) in 200 μl PBS ...
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bioRxiv - Immunology 2024Quote: ... adhered cells were incubated for 2 h at 37°C with 100 µL of 1% p-nitrophenylphosphate (2 mg/mL, Sigma-Aldrich) in AMP buffer (0.1 M ...
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bioRxiv - Cell Biology 2023Quote: ... the aortic tissue was incubated for at least 12 h in Roti®Histol (#6640.4, Carl Roth) at RT and embedded in paraffin (#P3558, Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Host embryos were prepared by thawing frozen embryos and incubating them for 24 h in an M16 medium (M7292, Sigma-Aldrich). Further ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed in 100% methanol for 1.5 h and cleared in BABB (1:2, benzyl alcohol:benzyl benzoate) (Sigma-Aldrich, 108006, B6630).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated by 10% SDS-PAGE (90 V, 30 min; 120 V, 1 h) and transferred to a Polyvinylidene Fluoride (PVDF, Millipore, IPVH00010) membrane (290 mA ...
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bioRxiv - Immunology 2023Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 10 dpi and ending at 34 dpi.
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampal slices were post-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 24 h and transferred to 30% sucrose (Sigma-Aldrich). Slices were sectioned coronally at 20 μm and stored at −20 °C in cryoprotectant (20% glycerol ...
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bioRxiv - Developmental Biology 2023Quote: ... #3 and #4 were pooled and processed to obtain a single cell suspension through mechanical disaggregation followed by 2 h of enzymatic digestion in DMEM + 125 μg/mL collagenase (Sigma-Aldrich), 0.25 µg/mL dispase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Fully grown oocytes were harvested from female mice at 28–35 days of age that were superovulated 44 h later by injection of 5 IU of PMSG in 37 °C pre-warmed M2 medium (M7167; Sigma-Aldrich) and cultured in M16 medium (M7292 ...
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bioRxiv - Developmental Biology 2024Quote: Total mRNA was extracted from larval brains of control (yw) and mrtf[Delta7] at 24 h ALH using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were restimulated after 72 h of culture with PMA (50 ng ml-1) and ionomycin (1 µg ml-1, both from Sigma-Aldrich) in the presence of brefeldin A (5 µg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... A fraction of the cell lysates was used to estimate NAD(H) levels using the NAD/NADH quantification kit (Sigma MAK037) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Stationary phase cultures (37°C, 18-24 h, with shaking) were washed twice with sterile phosphate buffered saline (PBS; D8537, Sigma-Aldrich) and diluted to 5 x 105 CFU.mL−1 for experiments ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Biochemistry 2023Quote: ... 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of TEV protease (10 U/µL) (T4455, Sigma-Aldrich) in SEC buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mg/mL of streptomycin (P/S) (Cat# 4-01F00-H, BioConcept) and 10 % fetal bovine serum (FBS) (Cat# S0615, Sigma-Aldrich) at 37 °C in a humidified atmosphere with 5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Genomics 2022Quote: ... and 40 h later treated with 2 μg/mL doxycycline hyclate or seeded in 96-well plates at 3e3 cells per well and 24 h later treated with 1 μg/mL doxycycline hyclate and either DMSO or 10 μM MG132 (Sigma-Aldrich). Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... at 50 μM and 100 μM concentrations while H+-dependent motility was inhibited using the protonophore Carbonyl cyanide 3-chlorophenylhydrazone (CCCP, C2759 Sigma-Aldritch).
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg dsRNA were added and incubated for 1 h at 27°C, before adding 1 ml full medium (ExpressFive SFM Invitrogen, 16 mM Glutamine, 10% FBS (Sigma-Aldrich), 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were incubated for 1 h with 1 μg/ml DAPI and 50 pmol Atto 550-conjugated Phalloidin (19083, Sigma-Aldrich). Samples were washed and imaged in PBS images were acquired with a confocal laser scanning microscope (Leica TCS SP8X ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes, Sigma-Aldrich). The PLA probes that bind to the constant regions of the primary antibodies contain a unique DNA strand ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 2 mM thymidine for 24 h followed by release from thymidine into fresh medium containing 100 ng/ml nocodazole (Sigma, M1404) for 18 h ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... for 1 h and for inhibition of stress granule formation cells were treated with 1.5 µg/ml of ISRIB (Sigma-Aldrich, SML0843). Stable HeLa cell lines constitutively expressing human marker proteins for membraneless compartments ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...