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Citations for Millipore Sigma :
1601 - 1650 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Expression of FLAG-importin α WT or S62A was induced for 16 h by addition of 1 µg/ml doxycycline (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... cells were starved in charcoaled stripped medium for 48 h and stimulated for 5 minutes with 100 nM β-estradiol (Sigma) prior to the measurements ...
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bioRxiv - Developmental Biology 2021Quote: ... Cumulus-oocyte complexes were collected from the oviduct of wild-type female C57BL/6 mice at 16 h after ovulatory human chorionic gonadotropin (hCG, Sigma-Aldrich) injection and placed in a 50 µL drop of TYH medium that was then covered with mineral oil (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... was added and incubated at 37°C for 1 h at 200 rpm and subsequently spread on LB-AMP (LB supplemented with 20 μg/ml Ampicillin (Sigma, A9518)) agar plates and incubated at 37°C ON ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Membranes were blocked in PBS + 0.1% Tween 20 + 5% powdered milk for 1 h and then incubated with anti-HA (Sigma-Aldrich, H6908) or Anti-Actin monoclonal antibody made in mouse (ThermoFisher Scientific AM4302 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated at 37ºC for 1 h with Duolink® in situ PLA® Anti-rabbit PLUS and Anti-mouse MINUS (Sigma) and later washed with 0,01% PBS-tween ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the leaves from five-week-old Col-0 plants were sprayed 6 times over 48 h with methyl jasmonate (MeJA) (Sigma-Aldrich, Cat # 392707 ...
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bioRxiv - Developmental Biology 2020Quote: ... Sections were blocked with Blocking One Histo (Nacalai) for 1 h at room temperature and then incubated with primary antibodies (α-γH2AX (05-636-AF647, Millipore) and α-MYBL1 (NBP1-90171 ...
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bioRxiv - Developmental Biology 2020Quote: ... good heart rate and blood circulation was verified under a stereoscopic microscope for culturing the embryos for additional 15 h in the presence of the autophagy inhibitor spautin-1 (SIGMA, SML0440) or the senolytic ABT-263 (Chemgood ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287, 4-nitroquinoline 1-oxide, Sigma N8141 ...
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bioRxiv - Neuroscience 2022Quote: ... susceptibility to S-IRA in primed DBA/1 mice was confirmed 24 h prior to treatment with 5-HTP (Cat #107751; Sigma-Aldrich) or vehicle ...
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bioRxiv - Cancer Biology 2022Quote: ... washed in PBS at room temperature for 1 h and cryoprotected in 15% w/v sucrose (Sigma Life Science, cat. S9378) followed by 30% w/v sucrose at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were permeabilized with 1% Triton X-100 (1 h, RT) and then incubated in 0,1% Triton X-100 and 1% bovine serum albumin (BSA, Sigma-Aldrich, A7906) for 1,5 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... washed as above described and re-blotted using mouse anti-β-actin antibody (Sigma-Aldrich, USA; 1:2500, 1 h, RT). Again ...
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bioRxiv - Immunology 2021Quote: ... plated in 96-well at a concentration of 2×105 cells/well and cultured for 72 h with medium alone or with OVA V (5 μg/ml; Sigma-Aldrich). Their supernatants were analyzed for cytokine expression using the V-Plex proinflammatory panel 1 mouse kit (MesoScaleDiscovery ...
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bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Genomics 2021Quote: ... the average tumor diameter reached 10 mm, mice were treated for 96 h with 2 mg/ml dox (belapharm, Vechta, Germany) in sucrose (Sigma-Aldrich) drinking water or only sucrose drinking water ...
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bioRxiv - Biophysics 2021Quote: ... flat 1:30 PDMS stamps were incubated for 1 h with 40 mL of 50 mg/mL Alexa Fluor 647–labeled and 50 mg/mL unlabeled fibronectin (F1141; Sigma-Aldrich), then washed and dried ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was pre-cleared with agarose beads (1 h, 4 °C) and incubated with 50 µl of saturated anti-V5 beads (Sigma A7345) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Cancer Biology 2020Quote: ... washed in warm PBS and then chased for 18 h without radioactivity in DMEM containing 10% FBS and 10 mM valine (Sigma-Aldrich), to allow degradation of short-lived proteins as previously described67 ...
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bioRxiv - Neuroscience 2020Quote: ... The polymer used to form the PLGA microspheres was 50:50 lactic acid to glycolic acid with 29 kDA molecular weight (Resomer RG 503 H; Sigma-Aldrich). An oil-in-water emulsion with solvent evaporation technique as previous described 14,52,53 ...
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bioRxiv - Bioengineering 2021Quote: ... We sterilized the micropatterned molds under UV for 1 h and then treated them with 1% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 1 h at room temperature to prevent the hydrogel from sticking to the PDMS mold ...
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bioRxiv - Bioengineering 2021Quote: ... samples were blocked for 1 h in 1% (w/v) bovine serum albumin and incubated with mouse monoclonal anti-vinculin antibody (1:1000, Sigma #V9264) or mouse monoclonal anti-VE-cadherin antibody (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Neuroscience 2021Quote: ... after 3 DIV the medium of some cultures was replaced for 2 h by fresh medium devoid of B-27 and GlutaMAX-I supplement and then cells were incubated for 2 h with 10-10 M E2 (Sigma-Aldrich) or vehicle ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were first incubated for 1 h at room temperature in a blocking solution containing 4 % bovine serum albumin (Sigma-Aldrich) in PBS-T ...
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bioRxiv - Cancer Biology 2021Quote: ... After 10 days constructs were fixed in 4% paraformaldehyde and embedded in paraffin and stained with H&E and stained for Fibronectin with FN1 antibody (F3648, Sigma Aldrich). For each construct the number of cells invading into the collagen was counted in at least five different fields of view under light microscopy ...
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bioRxiv - Cell Biology 2021Quote: ... nonfat dry milk in PBS-T for 1 h Membranes were incubated with mouse anti-FLAG M2 antibody (1:500, Sigma-Aldrich) or mouse anti-α-tubulin B512 antibody (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Neuroscience 2020Quote: ... The next day sections were washed in PBS and incubated for 1 h with A594-conjugated secondary donkey anti-goat antibody (1:500, EMD Millipore). Sections were then washed in PBS and blocked with 10% normal goat serum (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... DIV10 neurons were cultured in amino acid-modified (see above for description) or standard culture medium for 48 h post-transfection and then exposed to 2 μM puromycin (Millipore, 540222) for 10 min at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... blocked and permeabilized for 1 h in 10% donkey serum/PBS-T/0.3% triton-X-100 pH 7.4 and incubated for 2 h (RT) in mouse anti-puromycin 12D10 antibody (Sigma Aldrich, MABE343), washed extensively ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
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bioRxiv - Microbiology 2020Quote: Seven-week old Arabidopsis leaves with or without herbivore infestation for 6 h were stained with 1 mg/ml DAB-HCl solution (Sigma, D5637) overnight in darkness ...
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bioRxiv - Systems Biology 2020Quote: ... diluted 1:500 in 1% BSA with 0.3% Triton X-100 for 1.5 h in RT and stained with 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma Aldrich, #D9542) for 10 min in RT ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... cells were either left unstimulated or activated for 4 h with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (500 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating tissue sections were washed three times for 10 minutes with PBS then incubated for 2 h in a PBS-blocking solution containing 0.5 % Saponin (Sigma Aldrich 84510) and 10 % pre-immune goat serum (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... Axonal ChR2 expression was revealed by incubating slices for 1-2 h in Triton PBS containing 10% v/v NDS (D9663, Sigma, RRID:AB_2810235), then overnight at room temperature in Triton PBS containing 1% v/v NDS and Alexa Fluor 488-conjugated rabbit anti-GFP (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Virus supernatants were removed 7 h post-spin inoculation and cells were maintained in fresh growth medium for 48 h prior to selection in 3 μg/mL puromycin (Sigma-Aldrich). Stable cell lines were generated after 8 days of selection.
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were washed and incubated for 1 h at room temperature with primary antibody (anti-HA, mouse, 1:500, Sigma-Aldrich) diluted in PBS containing 1 % bovine serum albumin ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891).
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bioRxiv - Cell Biology 2020Quote: ... puromycin lentiviral transfer vector expressing 3x myc tagged V737N β1 integrin using the tetracycline rtTA2(S)-M2 (92) inducible promoter for 24 h with doxycycline [200 ng/mL] (Sigma, D9891). No respiratory repression of MCF10A or MB-MDA-231 cells was observed with 200 ng/mL ...