Labshake search
Citations for Millipore Sigma :
1651 - 1700 of 2153 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Medium was changed every 24 h and plates were stained for alkaline phosphatase activity to assure the efficiency of reprogramming (AP detection kit, Sigma-Aldrich). Once colonies were picked ...
-
bioRxiv - Cancer Biology 2022Quote: ... The membrane was blocked for 1 h with 5 % milk in TBST and blotted for anti-ssDNA (1:1000, Millipore, MAB3034).
-
bioRxiv - Immunology 2022Quote: ... 2 × 105 MOs were stimulated for 24 h at 37 °C and 5% CO2 with 1 μg/mL LPS (Escherichia coli 0127:B8, Sigma-Aldrich). After LPS elimination ...
-
bioRxiv - Neuroscience 2022Quote: ... the membrane was incubated for 12 h at 4°C with the primary antibody: anti-VGlut1(AB5905, Millipore, Burlington, 1:2000), anti-PSD95 (51-6900 ...
-
bioRxiv - Biophysics 2022Quote: ... The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich) was supplemented in the culture to a final concentration of 30 μM ...
-
bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were transferred to black-walled 96-well plates (Falcon) 4 h before the assay and incubated with EBM or 200nM Staurosporine (Sigma Aldrich) for 4 h ...
-
bioRxiv - Biochemistry 2022Quote: ... U937 cells were differentiated to macrophages by 24 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24 h incubation in RPMI medium ...
-
bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
-
bioRxiv - Pathology 2022Quote: ... The cultured C3H/10T1/2 cells were infected with 5 ml of retroviral supernatant for 48 h with 10 µg/ml Polybrene (Sigma-Aldrich).
-
bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
-
bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 56 d at 4°C ...
-
bioRxiv - Bioengineering 2022Quote: ... a 96-well plate was seeded with 1×104 L929 murine fibroblast cells per well and incubated for 24 h to allow for cell adhesion and spreading (L929 cell line was purchased from Sigma-Aldrich, Poznań ...
-
bioRxiv - Cancer Biology 2022Quote: ... twice within 48 h and then selected with 1 μg/ml puromycin (Sangon Biotech, Shanghai, China) or 200 μg/ml G418 (Sigma–Aldrich) for 10 days ...
-
bioRxiv - Cell Biology 2022Quote: ... in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich) for one hour ...
-
bioRxiv - Cell Biology 2022Quote: ... were incubated for 4 h in the capacitation medium supplemented or not with different concentrations of cycloheximide (CHX; C7698, Sigma-Aldrich) or chloramphenicol (CP ...
-
bioRxiv - Cancer Biology 2022Quote: ... tumor tissue was minced and digested at 37°C for 12 h in a dissociation buffer containing 0.012% (wt./vol.) collagenase XI (Sigma-Aldrich, C7657) and 0.012% (wt./vol. ...
-
bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
-
bioRxiv - Immunology 2021Quote: ... Monomers were loaded with 0.2 mg/ml peptide at 37°C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma-Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... were mixed with 100 μl of individual peptide pools in 96-well plates and incubated at 37°C for 1 h after which brefeldin A (Sigma Aldrich) was added and incubation continued for 4-5 h ...
-
bioRxiv - Cell Biology 2021Quote: ... the supernatants obtained were incubated with gentle rotation for 1 h at 4°C with anti-FLAG tag-antibody-conjugated magnetic beads (Sigma-Aldrich). The beads were then washed three times with lysis buffer #1 and incubated with gentle rotation for 30 min at 4°C with 50 μL of an elution buffer (50 mM HEPES-KOH ...
-
bioRxiv - Microbiology 2021Quote: ... The proteins were eluted using 250 µg/mL Flag peptide into wash buffer for 1 h at 4°C and subjected to immunoblotting analysis using antibodies for Flag tag (SIGMA, SLCD6338) and SARS-CoV-2 S (Sino Biological ...
-
bioRxiv - Immunology 2020Quote: ... To activate the NLRP3 inflammasome BMMs were first primed with LPS in cell culture medium or left unstimulated for 3 h before cells were treated with 5 µM nigericin (Sigma-Aldrich) for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then treated for 15 min up to 4 h at 37°C with 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, #M5655-1G) in 5 mM PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... in TBST for 1 h at room temperature (RT) and incubated with primary antibodies against β-actin (mouse, 1:50,000, Sigma, A5316) and Myosin Light Chain 2 (rabbit ...
-
bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
-
bioRxiv - Immunology 2020Quote: ... cells were treated for 1 h with a range of concentrations of the DNA methylating agent methyl methanesulfonate (MMS) (Sigma-Aldrich), and subsequently cultured for an additional 48 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... were used to infect subconfluent MCF7 KRT19 KO cells in three sequential 4 h incubations in the presence of 4 μg/ml polybrene (Sigma-Aldrich). Transductants were selected in hygromycin (100 μg/ml) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Virus-containing supernatants were collected 48 h later and added with Polybrene to AML cells pre-seeded at ∼5 × 105/well in 24-well plates (Sigma-Aldrich). After 8 h ...
-
bioRxiv - Biochemistry 2021Quote: ... They were then blocked for 1 h with 10% normal goat serum (NGS) in 0.1% TBS-Ta (TBS containing 0.1% Triton-X, Sigma, St. Louis, MO). Primary antibodies against cre recombinase and rhodopsin (see Table 2 ...
-
bioRxiv - Neuroscience 2022Quote: ... for 2 h at room temperature and finally counterstained for 1 min with Hoechst 33258 (10 μg/mL in PBS, Sigma-Aldrich). Images were taken using a confocal microscope LSM-800 Zeiss (28148) ...
-
bioRxiv - Genetics 2022Quote: ... Bb was cultured at 37ºC in Barbour-Stonner-Kelly with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer media (BSK-H) complete with 6% rabbit serum (Millipore Sigma) and 1% Amphotericin B (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were fixed in 70% ethanol for 1 h and stained for 10 min with an ALP staining solution (BCIP/NBT alkaline phosphatase kit, Sigma-Aldrich), according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Donor strains were grown in 96-well deep-well plates (1-mL cultures in TY medium) for at least 5 h with shaking at 37 °C and a Breathe-easy (Sigma-Aldrich) film covering the plate ...
-
bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... 1:400) for 2-h at room temperature and then stained with 4,6-diamidino-2-phenylindole dihydrochloride (DAPI, #D9542, Sigma, Darmstadt, Germany) for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore; 1:300 ...
-
bioRxiv - Physiology 2020Quote: ... Cells were then incubated for 48 h in the presence or absence of 400 μM sodium oleate or linoleic acid sodium salt (≥98%, Sigma-Aldrich) in DMEM supplemented with 0.5% BSA and 1% penicillin-streptomycin ...
-
bioRxiv - Microbiology 2020Quote: ... Cleaned tissue samples were incubated at 37 °C for 1 h in 10 mL DPBS with 1 mM DTT and 3 mM EDTA (Sigma-Aldrich). After incubation ...
-
bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 3% BSA in PBS-T buffer for 1 h at room temperature followed by incubation with Streptavidin-HRP (Sigma-Aldrich) (1/25,000 ...
-
bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
-
bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
-
bioRxiv - Immunology 2021Quote: ... gondii tachyzoites (RH-LDM, MOI 2, 24 h) in presence or absence of GABA synthesis inhibitor semicarbazide (SC, 50 μM, Sigma-Aldrich). Cells were also challenged for 24 h with MOI-equivalent numbers of heat-inactivated (HI ...
-
bioRxiv - Microbiology 2020Quote: Cells were seeded in triplicate in 96-well plates and subjected to the indicated treatments for 48◻h before adding MTT reagent and measuring the absorbance at 57025FBnm according to the manufacturer’s instructions (Sigma, USA). For combination matrices ...
-
bioRxiv - Bioengineering 2021Quote: ... The flask was then completely covered with aluminum foil and the solution was left to stir overnight (approximately 18 h) at RT when it was quenched with 18 mg hydroxylamine hydrochloride (Sigma-Aldrich). The solution was dialyzed with molecular weight cut-off membranes (SnakeSkin™ Dialysis Tubing ...
-
bioRxiv - Genetics 2020Quote: ... The membrane was pre hybridized for 5 h at 55 °C in hybridization solution (PerfectHyb™ Plus Hybridization Buffer, Sigma Aldrich). Hybridization of the respective radio-labeled probe was carried out overnight at 55 °C ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed twice with medium and then incubated for 1 h in medium containing 100 μg/ml gentamicin (Sigma Aldrich) to kill extracellular bacteria ...
-
bioRxiv - Cancer Biology 2020Quote: ... H-3300) for 30 min in a steamer and inactivation of endogenous hydrogen peroxidase activity with 10% H2O2 solution (Sigma, H1009), followed by blocking using 10% Normal goat serum (Jackson Immuno Research ...
-
bioRxiv - Developmental Biology 2021Quote: ... hearts were transferred in 50% glycerol for 1 h and then mounted in anti-fade mounting media solution containing 100% glycerol + 20%(w/v) n-propyl gallate (Sigma P3130).
-
bioRxiv - Pathology 2021Quote: ... cryosections were transferred to TBS and incubated for 1 h at 37 °C with anti-mouse PLUS and anti-Rabbit MINUS PLA probes (Sigma-Aldrich). Then ...