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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for Mouse DTW Domain Containing Protein 2 DTWD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Lysate containing 200 ug of protein was applied to each Microcon-30 Ultracel PL-30 Regenerated Cellulose 30000 NMWL (Millipore, MRCF0R030) filter unit (1 mg of protein per replicate using 5 filter units in total ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with buffer H containing 0.1 M NaCl and 100 μg/mL of FLAG peptide (Sigma Aldrich, #F3290). Peak fractions were pooled and loaded onto a MonoQ 5/50GL column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... was diluted to 0.01 mg/mL concentration in 100 µL of 25 mM Tris/HCl buffer containing various concentrations of SAHS proteins or BSA (Sigma-Aldrich). 50 µL of each sample was stored at 4°C while the other 50 µL was dried using a Savant SPD131DDA SpeedVac (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... the remaining chromatin was divided amongst separate tubes containing protein A dynabeads pre-incubated with either 2μl anti-H3K4me3 (Millipore 07-473), 10μl anti-H3K27me3 (CST 9733 ...
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bioRxiv - Bioengineering 2023Quote: ... 10 µg of protein per sample were digested in a solution containing 100mM triethylammonium bicarbonate at pH 8.5 (TEAB) (Sigma Aldrich, T7408), benzonase nuclease (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The protein-containing fractions were pooled and concentrated in an Amicon spin concentrator of 10-30kDa cutoff (Millipore, UCF901024 and UCF903024) and buffer exchanged against Buffer B (20 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... The eluted fractions from solution C (containing target proteins) were applied to an Amicon Ultra 15 Centrifugal Filter (Millipore Billerica MA) for desalting and imidazole removal by using a buffer containing 100 mM Tris–HCl (pH 7.9) ...
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bioRxiv - Cancer Biology 2021Quote: ... transduced cells were selected by incubating the monolayer in DMEM:F12 (1:1)+10% FBS containing 2 μg/mL Puromycin dihydrochloride (Sigma, USA) for 24 h ...
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bioRxiv - Biophysics 2020Quote: ... cells were washed three times with 2 mL phosphate-free HEPES-buffered Hanks’ balanced salt solution (HHBSS) containing 20 mM HEPES (Sigma), pH 7.4 and resuspended in 1.5 mL of the same buffer ...
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bioRxiv - Systems Biology 2020Quote: ... was washed out and the cells were washed and resupended in 2% galactose YP media containing 15µg/ml nocodazole (Sigma-Aldrich) and incubated shaking for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... Plates were coated with 100 μl of 0.02% sodium carbonate (pH 9.6) containing 2 μg/ml fibronectin (Sigma-Aldrich, USA) or 100 μl of phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
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bioRxiv - Neuroscience 2019Quote: ... Following permeabilization cells were blocked for 1h using 2% bovine serum albumin (BSA) in PBST (PBS containing 0.05% tween 20; Sigma, P2287). Primary antibody against hKir 4.1 (1:100 ...
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bioRxiv - Microbiology 2019Quote: ... and then cultured for 24 h in serum-free media containing the amounts reported in Results of the glycolysis inhibitor 2-deoxy-D-glucose (2DG; D3179, Sigma), the glutaminase inhibitors BPTES (314045 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... wells were washed with embryo media before the fish were incubated in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma-Aldrich) to stain for neuromast mitochondrial activity for 20 minutes ...
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bioRxiv - Immunology 2019Quote: ... 500 ul of supernatant was transferred to a new 2 ml eppendorf tube containing 200 ul of 0.5 mm acid-washed glass beads (Sigma-Aldrich) and 500 ul of phenol:chloroform:isoamyl alcohol ...
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bioRxiv - Molecular Biology 2019Quote: In vitro BBB models were constructed and 1ml of serum-free EBM-2 medium containing 10 μg/mL HRP (0.5 mmol/L, Sigma-Aldrich) culture medium was added into the upper champer of the transwell system ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: pLKO.1 plasmids containing short hairpin RNA (shRNA) sequences targeting cadherin-11 or cadherin-2 were obtained from Sigma-Aldrich together with a scrambled negative control ...
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bioRxiv - Microbiology 2019Quote: ... Parasites were diluted to 0.3 parasites/100 μl and 100 μl of 2 % haematocrit culture was transferred to 96 flat-bottom plates in culture medium containing 200 mM L-glutamax (Sigma). After 7 days the media was changed and 0.2 % fresh blood added ...
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bioRxiv - Immunology 2019Quote: ... Spleens and lymph nodes were cut into small pieces and digested in IMDM (2% FCS) containing collagenase type IA (1 mg/ml, Sigma) and DNase I (10 U/ml ...
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bioRxiv - Plant Biology 2019Quote: ... A single colony of Pst was grown in 2 mL of LB medium containing 25 μg/mL rifampicin (Sigma-Aldrich). A single colony of Psta from a freshly streaked 3-day-old agar plate was grown in 50 mL of LB medium containing 25 μg/ml rifampicin and 50 μg/mL kanamycin (IBI Scientific ...
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bioRxiv - Immunology 2019Quote: ... Embryos/larvae were kept in egg water (60 ug/ml Instant Ocean sea salts) containing 0.003% 1-phenyl-2-thiourea (PTU, SIGMA-ALDRICH) at 28.5°C and treated with 0.02% ethyl 3-aminobenzoate methanesulfonate (Tricaine ...
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bioRxiv - Genetics 2021Quote: ... and 0.1% (w/v) SDS) containing 1× homemade protease inhibitor cocktail (1 mM 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (Sigma; A8456), 0.3 μM Aprotinin ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... pH 7.4) containing 10 mM sodium azide and 6 mM 2-deoxy-D-glucose (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 50 min ...
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bioRxiv - Immunology 2021Quote: ... 106 cells per sample were cultured in RPMI containing 2% FBS and stimulated with PMA (50 ng ml−1, Sigma), ionomycin (2 μg ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was re-suspended in ice-cold 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulphonic acid) buffer (pH 8.0) containing complete protease inhibitor cocktail (Sigma, USA) and PMSF (phenylmethanesulphonyl fluoride ...
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bioRxiv - Neuroscience 2020Quote: ... Worms were transferred at the L4 larval stage to NGM plates containing 50 μM 5-fluoro-2’deoxyuridine (FUDR, Sigma). The day of the timed egg laying was considered day 0 for lifespan analysis.
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bioRxiv - Bioengineering 2020Quote: ... and cells were fed with a continuous flow at 1 Psi of HS media containing 2% glucose and 0.001% Fluorescence Brighter 28 (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Immunology 2020Quote: ... from 1:50 to 1:51200 in PBS containing 2% skimmed milk were added followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... which were diluted in the fluorescence dilution buffer (PBS supplemented with 5% FBS and 2% bovine serum albumin) containing 0.1% saponin (Sigma-Aldrich). After extensive washing with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Neuroscience 2019Quote: ... Postfixed for 2 h with 1% (w/v) osmium tetroxide (TAAB Lab) containing 0.8% (w/v) potassium hexacyanoferrate (Sigma-Aldrich) in PB ...
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bioRxiv - Cell Biology 2021Quote: ... the rest pieces were digested by shaking in 2 mL of 5% (v/v) FBS in RPMI medium containing DNase I (Sigma) (150 μg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.5 mL of cell culture was transferred into a 2 mL Eppendorf Safe-Lock Tubes containing 0.1 g glass beads (Sigma, G8772) and 0.5 mL of ethyl acetate ...
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bioRxiv - Systems Biology 2021Quote: ... 5-10 L4 worms of each strain were transferred onto RNAi plates (containing 2 mM IPTG and 25 mg/mL carbenicillin) or RNAi plates containing 15 μg/mL Doxycycline (Dox, Cat. D9891, Sigma) seeded with E ...
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bioRxiv - Microbiology 2020Quote: ... and bacterial nucleoids stained by topping cells with a drop of Fluoroshield™ containing 1.5 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, Sigma). Cover slips were placed over the samples and kept at room temperature for ∼ 30 minutes before cells were imaged.
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bioRxiv - Immunology 2021Quote: ... Overnight cultures were centrifuged at 4,000 × G for 10 min at room temperature and resuspended in YNB-CAA containing 2 % galactose (Sigma Aldrich) to an OD600 of 1.0 to induce protein expression ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... was performed by intraperitoneal (IP) injection of 0.5 ml bacterial suspension containing 2×107 CFU/ml and 5% (wt/vol) mucin (Sigma-Aldrich). During experiments ...
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bioRxiv - Microbiology 2020Quote: ... 2 g/L drop-out mix synthetic minus uracil without yeast nitrogen base (US Biological)) containing 2% (wt/vol) glucose (Sigma) and incubated at 30 °C for 24 h with shaking (250 rpm) ...
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bioRxiv - Cancer Biology 2021Quote: ... 350mg of tissue was minced to generate 2 mm3 fragments and placed in RPMI1640 containing 200μg/ml of collagenase type IV (ref: C4-BIOC,Sigma-Aldrich) and 50U/ml of DNase I (11284932001 ...
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bioRxiv - Biophysics 2020Quote: ... Muscle pieces were thawed in base rigor buffer K60 (60 mL KCl, 2 mM MgCl2, 20 mM MOPS pH 7.4) containing protease (Sigma#8340) and phosphatase inhibitors (SigmaP0044) ...
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bioRxiv - Cancer Biology 2021Quote: Samples were transferred to a 2 ml tube containing 200 mg of ≤106 μm glass beads (Sigma, St. Louis, MO) and 0.5 ml of Qiagen PM1 buffer (Valencia ...
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bioRxiv - Biochemistry 2022Quote: ... cells were washed in 60% isopropanol and stained in an ORO solution (2:3 v/v H2O: isopropanol, containing 0.5% ORO, Sigma O0625) for 1 hour ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The aqueous layer was transferred to a fresh tube containing 2 μL Glycogen (20 mg/mL Sigma cat. no. 10901393001). DNA was precipitated with three volumes of 100% ethanol and centrifuged at 16,000xg for 30 min at 4°C. ...
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bioRxiv - Bioengineering 2022Quote: ... Twenty-thousand reporter cells were added to wells using 200 µL of DMEM media containing 2 µg/mL of doxycycline (Sigma) to induce Notch-Gal4 expression ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were then washed four times with lysis buffer and eluted in 2x Laemmli buffer containing 1% 2-mercaptoethanol (Sigma) by heating at 95°C for 3min.
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Pathology 2021Quote: Left lungs were put in 2 ml microtubes with 1 ml prewarmed PBS containing 200 units of collagenase (Sigma, C9891) and 0.9 mM of calcium and magnesium ...
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bioRxiv - Microbiology 2021Quote: Test sera were heat-inactivated at 56°C for 30 minutes to destroy any complement activity and serially diluted 1:2 in cell culture media containing 1% foetal calf serum (Sigma). Virus was diluted to give 100-250 foci in the virus-only control and then added to the serum dilutions before incubation for 1 h at 37°C ...