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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were denatured for 5 min at 95 °C in Bolt LDS Sample buffer with 5 % v/v 2-mercaptoethanol (Sigma). 10-30 μg of proteins were loaded in Bolt 4-12 % Bis-Tris gels (Thermo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... slides were washed for 5 min in 0.1x SSC with 1% Triton X-100 at 62°C and counterstained with 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich) in antifade with DABCO (1,4-diazabicyclo (2.2.2)-octane ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Lentivirus transduction was performed by spinoculation at 1200 X g for 2 hours at 32° C in the presence of virus particles and 8 ug/ml polybrene (Millipore). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... sub-confluent mouse embryonic 3T3-J2 fibroblasts were treated for 2 hours with 4µg/ml Mitomycin C (Sigma-Aldrich, M4287), washed three time with PBS ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was concentrated using ultracentrifugation in an SW-28 rotor (25,000 rpm, 2 h at 4°C) on a 60% OptiPrep density cushion (Sigma-Aldrich). The pelleted viruses were resuspended in 10 mL TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged with 293T retroviral supernatant at 1000×g for 2 hr at 37°C in the presence of 4 µg/mL polybrene (Sigma). Following spin infection ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were grown at 28°C in E3 medium containing 1-phenyl-2-thiourea (0.003%, PTU, Sigma-Aldrich, P7629-10,) to avoid pigmentation ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... DAB (3-3’-diaminobenzidine, Sigma-Aldrich catalog no. D8001-10G) was dissolved in 0.1 N HCl at a concentration of 5.4 mg ml-1 and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Developmental Biology 2022Quote: ... The reaction was developed using 3-3’-Diaminobenzidine (DAB) (Sigma). For CLASP1 immunostaining ...
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bioRxiv - Cancer Biology 2019Quote: ... 3-hydroxybutyrate (3-OHB) was also purchased from Sigma (#166898).
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bioRxiv - Cell Biology 2019Quote: ... injected with 3 mL of 3% thioglycollate (Sigma-Aldrich, T9032). After 3 days ...
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bioRxiv - Immunology 2020Quote: ... E-64 and 3-Methyladenine (3-MA) were from Sigma. Necrostatin-1 was from Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC; Sigma Aldrich #39391), and 3NPH solutions were mixed ...
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bioRxiv - Neuroscience 2023Quote: ... (3) the PDE4B inhibitor A33 (3 mg/kg, Sigma-Aldrich), (4 ...
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bioRxiv - Immunology 2024Quote: ... 3-nitropropionic acid (3-NP, several doses, Sigma Aldrich N5636), Atpenin-A5 (several doses ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 10 mg/mL 3-iodo-L-tyrosine (3-IY, Sigma), and 0.5 mM all-trans-retinal for 2–3 days at 25°C in the dark prior to behavioral testing ...
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bioRxiv - Immunology 2021Quote: ... The membrane was blocked using 1x TBST+ 3% Casein followed by overnight incubation at 4°C with biotinylated Lectin from Triticum Vulgaris (Sigma/Merck Cat. No. L5142). The membrane was washed with 1XTBST and further Incubated with Streptavidin PerCP-eFluor710 Conjugated ...
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bioRxiv - Cell Biology 2019Quote: hMSCs were harvested with trypLE™ at 60 – 70 % confluence and a solution containing 6.105 cells in 70 μL medium were mixed with a 30 μL 3 % (w/v) liquid low melting agarose solution (i.e. stored at 37 °C) (Sigma-Aldrich, Saint Quentin Fallavier, France) diluted in culture medium containing 50 μg/mL Gentamicin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... parahaemolyticus strains were grown in lysogeny broth (LB) medium (Fischer Scientific, Pittsburgh, PA) containing 3% NaCl (LBS) at 37°C with aeration or M9 medium media (Sigma Aldrich, St. Louis, MO) supplemented with 3% NaCl (M9S) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Microbiology 2022Quote: ... parahaemolyticus strains were grown in Luria Bertani (LB) medium (Fischer Scientific, Pittsburgh, PA) containing 3% NaCl (LBS) at 37°C with aeration or M9 medium media (Sigma Aldrich, St. Louis, MO) containing 3% NaCl (M9S ...
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bioRxiv - Microbiology 2023Quote: ... Boost vaccination was performed with the homologous virus inactivated at 4°C for 3 days with 0.1% beta propiolactone (BPL) (Sigma-Aldrich Corporation, St. Louis, MO) as previously described (66) ...
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bioRxiv - Neuroscience 2020Quote: ... 3% BSA (Sigma), 0.3% Sodium azide (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... Nutlin-3 (Sigma) was made up to 20mM in DMSO and used at 5µM ...
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bioRxiv - Neuroscience 2020Quote: ... 3 (Sigma Aldrich). For the immunofluorescence staining of freshly extracted zfPGCs ...
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bioRxiv - Microbiology 2020Quote: ... 3% milk (Sigma). Primary antibody detecting ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% BSA (Sigma) and 0.1% Tween-20 (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mixture (Sigma). Cell lysates were then centrifuged at 15000×g for 20 min ...
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bioRxiv - Cell Biology 2019Quote: ... 3 hematoxylin (Sigma) for 60 s ...
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bioRxiv - Plant Biology 2019Quote: ... 3-MA (Sigma) was added to BY-2 cells at a final concentration of 5 mM ...
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bioRxiv - Neuroscience 2021Quote: ... Relaxin-3 (Sigma), prostaglandin E2 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 3% BSA (Sigma) in TBS ...
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bioRxiv - Cell Biology 2021Quote: ... 3% BSA (Sigma)) for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 3% sucrose (Sigma) and 0.8% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 3 (Sigma-Aldrich) and eosin Y (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... 3% BSA (Sigma), 0.5% triton X100 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... 3 (Sigma-Aldrich). Documentation was performed with an Axioskop 2 mot plus (Zeiss ...
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bioRxiv - Plant Biology 2019Quote: ... A ratio of 1:2 and 1:3 (v/v) polyvinylpolypyrrolidone (PVPP, ~110 µm particle size, Sigma-Aldrich, Saint Louis, USA) to ground tissue was used ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability was determined using a commercial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) viability assay (Sigma Aldrich, Rehovot, Israel). That assay was used to determine how highly 4T1 proliferation was stimulated by Norepinephrine (NE ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown for 3 days on coverslips until they reached confluence and then 50 ug/ml L-ascorbic acid-2-phosphate (Sigma Aldrich) was added to cultures ...
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bioRxiv - Cell Biology 2020Quote: ... All surrounding agarose was removed from the tissue and 2-3 tissue slices were positioned on each Millicell Cell Culture Insert (Merck Millipore, PICM0RG50) using No.22 scalpels (Swann-Morton ...
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bioRxiv - Genetics 2021Quote: ... was propagated and maintained by transferring shoot segments of 3-4 cm with 1-2 young leaves to fresh one-half Murashige and Skoog (Beijing, China, Sigma-Aldrich). Plantlets were grown at 23 ℃ under a 16 h light/8 h dark cycle with a light intensity of illumination of 5000 lux provided by cool white fluorescent lamp tubes ...
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bioRxiv - Biochemistry 2022Quote: ... by plating them on NB agar medium (2% glucose, Ade, Ura) supplemented with different concentrations (5-50mM) of 3-aminotriazole (3AT; Sigma-Aldrich) histidine inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue is then washed twice with 2-3 mL sterile-filtered DM+Trypsin Inhibitor (1 mg/mL; Sigma Aldrich, T9253) and incubated in the 3rd wash for 3-5 min at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... The gradient was fractionated from the top in 11-12 fractions using wide-bore pipette tips and peak 2-3 fractions were identified by immunoblotting against γ-tubulin with GTU-88 antibody (Sigma). γ-TuRC was concentrated to 80 μl in 30k NMWL Amicon concentrator (EMD Millipore ...