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Citations for Millipore Sigma :
1551 - 1600 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and L-leucine-7-amido-4-methylcoumarin (LAPase) (Sigma, Aldrich) were prepared according to Hoppe (1983 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: We selected BPA (CAS: 80-05-7, 99.5%, Sigma Aldrich), diethyl phthalate (DEP ...
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bioRxiv - Neuroscience 2024Quote: ... 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM, Sigma), D-APV (D-(-)-2-amino-5-phosphonvaleric acid ...
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bioRxiv - Immunology 2020Quote: ... DH10β and STBL3 for propagation of plasmids were cultured at 37°C C (30°C C for STBL3) in LB broth (Sigma-Aldrich) with shaking at 220 rpm.
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bioRxiv - Developmental Biology 2022Quote: Samples were incubated with primary antibody overnight at 4°C in PBT (PBS with 3% Triton X-100 (Sigma-Aldrich X100-100 mL)) with 5% NGS (Capralogics GS0250) ...
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bioRxiv - Biophysics 2021Quote: Methanol-fixed infected cells and a methanol-fixed negative control were incubated overnight at 4 C° with 150 μl of a solution of anti-S IgG or anti-N IgG in PBS + 3% BSA (Sigma-Aldrich, Milan, Italy). For colocalization experiments ...
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bioRxiv - Plant Biology 2019Quote: ... surface sterilized and stored at 4 °C for 3 days before growing on MS agar (1.1% w/v) containing 100 µg/mL kanamycin (Sigma-Aldrich, St. Louis, MO). The proportion of WT to dgk4-1 seeds were scored after 7 days of growth.
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bioRxiv - Cancer Biology 2021Quote: ... fresh sterile samples from resected tumors were minced in Dulbecco’s modified Eagle medium (DMEM; Corning, cat # 10013CV and incubated with collagenase type I (3%; Sigma-Aldrich, cat # C-0130), DNase I (0.02% ...
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bioRxiv - Microbiology 2022Quote: ... Both strains were initially grown anaerobically at 37°C for 3–5 days on Bifido Selective Media (BSM) agar plates (Millipore Sigma, Burlington, MA), from which a single colony was selected and grown in BSM broth (Millipore Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... SIGMA, #M9942, 1:200), (ki67, BD Pharmingen, #550609, 1:200), (C-caspase 3, Cell-signaling, #9661S, 1:100) (PCNT, SIGMA, #HPA016820, 1:200), (P-Histone H3 ...
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bioRxiv - Biochemistry 2023Quote: ... SP3 beads were then resuspended in 100 μL of 200 mM HEPES (pH 7.5) and digested overnight at 37 °C with Solu-trypsin (3 µg solu-trypsin, Sigma, trypsin:protein ratio 1:33). The resulting peptide mixtures were collected using a magnetic rack ...
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bioRxiv - Neuroscience 2021Quote: ... the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
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bioRxiv - Biophysics 2022Quote: ... The clean coverslips were silanized by incubating in a solution of 2% (v/v) 3-aminopropyl-triethoxysilane (Sigma-Aldrich A3648) in acetone for 30 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... or 1000 nM of 2-(4-amino-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-3-yl)-1H-indol-5-ol (PP242; Sigma, P0037), (2 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cytotoxic effect was assessed using in MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay (Sigma- Aldrich, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
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bioRxiv - Bioengineering 2021Quote: ... 200 mg Ru(dpp)3(ClO4)2 complex was dissolved in 10ml ethanol (ACS reagent ≥99.5%, CAS 459844; Sigma-Aldrich). Silica gel was prepared by adding 2 g silica particles to 40 ml aqueous NaOH (0.01N ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in B2 with 2% nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP, Sigma) until adequate colour development ...
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bioRxiv - Cancer Biology 2020Quote: ... 50mM Tris pH 8.0 and cOMPLETE™ Mini protease inhibitor cocktail (#11836153001) and phosphatase inhibitor cocktail 2 and 3 (Sigma) for lysis ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were seeded into 6-well plates to reach 50-60% confluency on the day of infection and were transduced 2-3 consecutive days with the viral supernatant in the presence of 8 μg/mL polybrene (Sigma). The viral supernatant was replaced with fresh culture medium ...
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bioRxiv - Plant Biology 2021Quote: ... PVDF membranes were blocked with 5% milk followed by an incubation with first antibodies for REM1.2/1.3 followed by 3 washes and an incubation with second α-rabbit antibody fused to HRP against rabbit (Sigma). Clarity Western 701 ECL (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... nicotinic acid (cat. N4126), 2-aminopyridine-3-carboxylic acid (cat. A68300) and 1,1′-Carbonyldiimidazole (CDI, cat. 21860) were purchased from Sigma Aldrich. For the synthesis of NAI ...
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bioRxiv - Plant Biology 2021Quote: ... green tissues of 2- to 3-week old plants were sprayed until drop-off with 1 mM SA (Sigma Aldrich), 100 μM MeJA (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Cell Biology 2020Quote: ... cells were replica-plated onto pABA− (2% glucose, w/v) plates with 0 µM or 25 µM linolenic acid (C18:3, Sigma). Colonies that grew on 25 µM linolenic acid were picked into YEPD overnight cultures and struck on YEPD plates ...
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bioRxiv - Biochemistry 2022Quote: ... cells were washed in 60% isopropanol and stained in an ORO solution (2:3 v/v H2O: isopropanol, containing 0.5% ORO, Sigma O0625) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... The surface was regenerated with two pulses (30 s at 100 µL/min) of Isopropanol:50 mM NaOH (2:3) solution followed by a 20 mM CHAPS (Sigma) or 40mM Octyl-beta-Glucoside (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... and then stained for 2 minutes at room temperature using filtered 0.5% Solvent Black 3 (CAS Number 4197-25-5; Sigma 199664) dissolved in 75% ethanol ...
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bioRxiv - Biophysics 2022Quote: ... 1-oleoyl-2-(12-biotinyl(aminododecanoyl))-sn-glycero-3-phosphoethanolamine (biotin-PE) and Tween-20 were purchased from Sigma Aldrich. Tween-20 was suspended in 50 mM Tris buffer (pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 1% protease inhibitor and phosphatase inhibitor cocktail 2 and 3 (all from Sigma Aldrich, St. Louis, MO, USA). Samples were centrifuged at 14,000 rpm for 20 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... 3 days post-fertilization (dpf) zebrafish larvae were anaesthetized for 2 - 3 minutes in embryo medium E3 containing 0.2 mg/ml Tricaine (Sigma-Aldrich). Thereafter ...
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bioRxiv - Microbiology 2020Quote: ... the medium was replaced with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma-Aldrich, St. Louis, MO, USA) final concentration at 0.5 mg/mL and incubated for 4 h at 37°C in a humidified incubator with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... next permeabilized for 20 minutes in PBST (PBS with 0.3% Triton X-100; BioShop) and blocked for 2 h in 3% BSA (Sigma-Aldrich) in PBST at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 µl of a solution of 5 mg/mL MTT [3-(4,5)-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma Chemical Co.) was added ...
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bioRxiv - Genetics 2022Quote: ... Slides were then rinsed in washing buffer five times for 3 min each and nuclei were stained with 4lr,6-diamidino-2-phenylindole (DAPI, cat # D-1388, Sigma) at a concentration of 1 μg/ml for 10 min at RT ...
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bioRxiv - Immunology 2019Quote: ... Fluorescein isothiocyanate (FITC) [2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-5-isothiocyanatobenzoic acid] was purchased from Sigma-Aldrich Korea ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using the set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) and by non-phosphorylated peptides identified in direct analyses.
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... and typically plated at 1:2-1:3 dilution ratio in neural differentiation media supplemented with RevitaCell on plates coated with poly-L-ornithine (Sigma) and laminin (1/1000 in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... injected embryos were dechorionated and embedded in 2-mm or 3-mm glass capillaries (Hilgenberg GmbH) filled with 0.9% low melting temperature agarose (Type VII, Sigma Aldrich) prepared in zebrafish system water ...
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bioRxiv - Plant Biology 2019Quote: ... was assessed by evaluating the cell viability by MTT assay using 3-(4,5-dim-ethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide reagent or MTT (Sigma-Aldrich®) [36] ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were exposed to 5 μM CHIR99021 (Axon) between days 2 and 3 and then supplemented with 100 nM RA (Sigma) until day 5 ...
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bioRxiv - Cell Biology 2019Quote: ... Normalization of the data was performed using a set of phosphopeptide standards (added to the sample prior phosphoenrichment step; MS PhosphoMix 1, 2, 3 Light, Sigma) in combination with summed peak area of non-phosphorylated peptides identified in analyses of samples without phosphoenrichment ...
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bioRxiv - Cancer Biology 2021Quote: ... after 72 hours of drug treatment, 20μl of a 5mg/mL 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (M2128-MTT; Sigma-Aldrich, USA) solution was added to each of the wells ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed with 3% PFA/PBS and the nuclei stained with 4’,6-diamidine-2’-phenylindole (DAPI, Sigma). An inverted fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2020Quote: Caco-2 cells were seeded at 2 × 105 cells/well onto polycarbonate Millicell culture-plate inserts (12-mm diameter, 3-μm pore size; Millipore) and cultured for 5 days ...
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bioRxiv - Neuroscience 2019Quote: ... 150 mM NaCl, 1% Triton-X100, 1X Complete protease inhibitor cocktail [Roche], 1X phosphatase inhibitor cocktails 2 and 3 [Sigma]). Protein concentration was determined using BCA assays (Pierce Biotech).
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bioRxiv - Cell Biology 2019Quote: ... 5’-GGTCAGCCAACTCGTCACAGTC-3’) and by alizarin red S staining with a 2% (w/v) alizarin red S solution (Sigma-Aldrich). Slides were imaged in an Olympus BX61 microscope ...
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bioRxiv - Neuroscience 2019Quote: ... sections were rinsed 3 times for 2 min in 0.01 M PBS containing 0.2% Triton X-100 (Tx) (PBS-Tx; Sigma-Aldrich, T8787) and then were pre-treated with 3% H2O2 in PBS-Tx for 10 min ...
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bioRxiv - Cell Biology 2019Quote: The viability of cells was determined using the standard MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (MTT, Sigma-Aldrich, PL). The cells were incubated on 96-well plate (Perkin Merkel ...
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bioRxiv - Neuroscience 2020Quote: ... from Tocris CGP-55845 ((2S)-3-[[(1S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydro xypropyl](phenylmethyl)phosphinic acid hydrochloride) from Sigma-Aldrich