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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 plaque reduction assays were evaluated by first staining plaques with .05% Neutral Red solution for 3 hours at 37 °C (Sigma Aldrich cat. no. N2889-100mL). Neutral red was then aspirated from the wells and plaques were counted ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% [v/v] Triton X–100) and incubated at 37°C for 3 h either with α–mouse plus and α–rabbit minus for PLA assay (Sigma–Aldrich, 681 Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution as described (Pasternak et al ...
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bioRxiv - Bioengineering 2024Quote: ... a separate cohort of tissue sections were pre-incubated for 30 minutes at 37◦C with either the NOX1 inhibitor ML171 (1 µM; CAS 6631-94-3, Sigma Aldrich; St. Louis, MO, USA) or the NOX2 inhibitor GSK (50 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Cell Biology 2020Quote: Cultured CT26 cells were collected and their viability was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St. Louis, MO, USA) colorimetric method ...
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bioRxiv - Neuroscience 2019Quote: ... BW723C86 (α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine monohydrochloride) and a primary antibody raised against β-actin were purchased from Sigma (USA). Other primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of freshly made 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 1 mg/mL; Sigma-Aldrich, St. Louis, MO) in serum-free ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Biochemistry 2019Quote: ... All other chemicals including palmitate and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) were obtained from Sigma-Aldrich (St. Louis, USA).
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bioRxiv - Developmental Biology 2021Quote: Larvae at 2-6 dpf were first anesthetized with 0.03 % Ethyl 3-aminobenzoate methane sulfonate salt (Sigma-Aldrich, St. Louis, MO, USA) and pinned onto a Sylgard-filled recording chamber (I-2450 ...
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bioRxiv - Cancer Biology 2021Quote: ... Viable cells were determined post treatment using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, USA; Cat# M2128) assay by measurement of absorbance at 570 nm.17 The relative rate of cell growth for each cell line was factored into the analysis by subtracting the absorbance at time 0 from both the control and treatment groups ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Biophysics 2020Quote: ... Purified horse heart cytochrome c (cyt c) was obtained from Sigma, bovine serum albumin was purchased from Roth ...
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bioRxiv - Neuroscience 2023Quote: ... anti-prosurfactant protein C (proSP-C, Millipore Sigma AB3786, RRID: AB_91588) was used at 1:4,000 with microwave antigen retrieval as described above ...
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bioRxiv - Genomics 2019Quote: ... and 12.5 ng/µL oligo(dT) at 42°C for 2 h in the presence of 6 ng/µL actinomycin D (Sigma, #A1410-2MG) to prevent antisense artifacts (Perocchi et al ...
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bioRxiv - Neuroscience 2020Quote: ... and postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate buffer (0.1M) ...
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bioRxiv - Neuroscience 2021Quote: EC sections were postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Neuroscience 2021Quote: Sections containing BA21 were selected and postfixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... the pellicles were first digested by shaking for 16 h at 4 °C in 15 ml of PBS buffer with 2% cellulase (Sigma Aldrich, C2730).
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bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were treated at the indicated doses with ionizing radiation (IR) or the next day incubated for 2 hours with mitomycin C (Sigma-Aldrich, M503) or for 24 hours with olaparib or cisplatin after which the cell media was refreshed ...
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bioRxiv - Neuroscience 2020Quote: EC sections were post-fixed for 24h in a solution containing 2% paraformaldehyde, 2.5% glutaraldehyde (TAAB, G002, UK) and 0.003% CaCl2 (Sigma, C-2661-500G, Germany) in sodium cacodylate (Sigma ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2020Quote: ... or with a solution of glycerol and DMSO in PB and were frozen with −60/-70°C isopentane (2-methyl butane, Sigma-Aldrich, Germany) (following the protocol of Rosene et al ...
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bioRxiv - Developmental Biology 2019Quote: ... Fertilized embryos were injected before first cleavage on agarose pads in a solution of pasteurized (30 minutes 65°C) filtered seawater (PFSW) + 2% w/v Ficoll 400 (Sigma F-9378). Embryos were hand-sorted for fluorescence between second and sixth cleavage cycles ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using blasticidin (2 µg/ml Sigma-Aldrich, Poland). Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... HCECs were grown in a hypoxia chamber with 2% O2 and 5% CO2 at 37°C in 4 parts DMEM to 1 part medium 199 (Sigma-Aldrich #M4530) with 2% cosmic calf serum (GE Healthcare) ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: ... we used a synthetic cathepsin C inhibitor (IcatCXPZ-01; (37) diluted in 10 % (2-Hydroxypropyl)-β-cyclodextrin (CAS: 128446-35-5; C0926; Sigma-Aldrich) suspended in citrate buffer 50 mM at pH=5 (vehicle ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants were removed and samples were digested with 2 ml trypsin (Sigma; 0.2% in PBS for 20 min at 37°C). Choroids were again centrifuged as described above ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Bioengineering 2023Quote: ... and vitamin C at a concentration of 50 μg/mL [16] (L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate, Sigma Aldrich, A8960). For the study of the effect of the tissue 3D geometry ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
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bioRxiv - Cancer Biology 2023Quote: The CaCl2 working solution was prepared by mixing 484.5 µL medium (see cell culture section) and 15.5 µL 2 M CaCl2 (Sigma Aldrich, cat# C-7902). This was followed by the preparation of the Annexin V solution consisting of 3 mL cell culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin from about 1.5×106 cells was incubated overnight at 4°C with 2–5 μg antibodies to FLAG epitope (M2; Sigma-Aldrich, #F1804, RRID:AB_262044), BRD4 (Cell Signaling Technology ...