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Citations for Millipore Sigma :
1901 - 1950 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin conjugates were eluted at 65 °C for 15 min in 2x Laemmli buffer containing 200 mM imidazole and 5% 2-mercaptoetanol (Sigma, M6250), vortexed for 90 seconds ...
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bioRxiv - Immunology 2020Quote: ... The DNA solution was heated at 50°C for 10 minutes and mixed with an equal volume of 2% agarose (Sigma-Aldrich). The mixture was poured onto a plastic casting tray and left at room temperature till it solidified ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were equilibrated at 37°C for 5 min and subsequently initiated by addition of NADPH (Sigma, final concentration 2 mM). The reaction was allowed to proceed at 37°C and timepoints at 0 ...
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bioRxiv - Immunology 2021Quote: ... or (2) primary anti-dsRed (Sicgen, AB8181-200, 1:200) together with primary rabbit anti-proSP-C (Sigma, AB3786, 1:1000) or primary rabbit anti-NaPi-IIb (provided by C ...
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bioRxiv - Immunology 2021Quote: ... and rotated at 37 C for 40 minutes in Collagenase IV Buffer + 2 mg/mL Dispase II (Sigma Aldrich cat. 04942078001). Homogenate was filtered through a cell strainer (Corning cat ...
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bioRxiv - Immunology 2021Quote: ... A375 and 786O-NL cells by spin-infection (500 g for 2 hours at 37°C) with 8 μg/mL polybrene (Sigma-Aldrich). Four days post-transduction ...
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bioRxiv - Genomics 2022Quote: ... bead/chromatin complexes were washed twice with Low Salt Wash Buffer at 4°C (Final 20 mM Tris-HCl, pH 8,0; 2 mM EDTA; 150 mM NaCl (Sigma-Aldrich, S6546); 1% Triton-X ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Dorsal root ganglia from one litter of 10 pups were treated at 37°C for 30 min in 2 mg/mL collagenase (Type IA; Sigma-Aldrich) and 5 mg/mL dispase II (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET), labeling mixture that was incubated for 10 min at room temperature in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... Soluble proteins were hatched for 10 min at 55°C with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, St. Louis, MO, USA), then alkylated for 15 min at RT in the dark with 10 mg/mL iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The fresh tissue was dissected followed by digestion for 2 hours at 37°C in 1 mg ml−1 Collagenase 1 (Sigma-Aldrich). Debris was removed and cells isolated by sequentially passing through 100 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
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bioRxiv - Biochemistry 2023Quote: ... the cell lysate was incubated (4 °C for 2 h) with anti-V5 agarose affinity gel antibody produced in mouse (Cat# A7345 from Millipore Sigma). After three repetitive washes (10 min each time ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Microbiology 2023Quote: The enzymatic production of DsrC-trisulfide was performed inside a Coy anaerobic chamber (98% N2, 2% H2) at 60 °C using Zn-reduced methyl viologen (Sigma-Aldrich) as the electron donor (7) ...
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bioRxiv - Cancer Biology 2022Quote: ... 12-well non-tissue culture treated plates were coated with 32 μg/mL retronectin (WEHI) in PBS solution overnight at 4°C then blocked with 2% bovine serum albumin solution (#A1595 Sigma-Aldrich) in PBS at 37°C for 30 min were prepared ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubated in a shaking water bath (180 rpm) at 37°C for 50 minutes with Type 1 Collagenase (2 mg/ml; Sigma-Aldrich) and DNase (100 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mg of the whole cell extract was incubated for 2 h at 4°C with 15 μl of M2-anti Flag agarose (Sigma- Aldrich), GFP-trap agarose (Chromo Tek ...
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bioRxiv - Immunology 2023Quote: ... Perfused lungs were dissected out and minced up prior to incubation in RPMI-1640 (2% FBS) + 0.3 mg/ml collagenase IV (Sigma, C-5138) + 0.3 mg/ml of DNase I (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... freezed 15 min at −80°C and thawed for 15 min at room temperature following overnight incubation in 2% TritonX-100 (Merck Millipore, 1086031000) PBS for permeabilization ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20°C for 30 min in the dark ...
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bioRxiv - Immunology 2024Quote: ... 2 x 106 cells per well were cultured in 200 μL supplemented RPMI 1640 medium and stimulated during 2 h at 37 °C with 50 ng/mL PMA and 1 µg/mL ionomycin (Sigma-Aldrich) in the presence of Brefeldin A and Monensin (eBioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... half of the medium was substituted with a culture medium supplemented with 2% B-27 plus supplement and 5μM of cytosine β-d-arabinofuranoside (Ara-C; Sigma; #C1768). The culture medium was then changed every three days ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were incubated 2 hours at 45°C in 0.1% Luxol Fast Blue (Solvent blue 38, Sigma Aldrich, catalog #S3352) in 95% Ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... they were cryopreserved for 48h at 4°C in a 30% sucrose solution and subsequently frozen with 2-methylbutane solution (Sigma-Aldrich). Parallel free-floating coronal sections (30-µm-thick ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Phytagel C (Sigma), and Gellan (PhytoTechnology Laboratories) ...
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bioRxiv - Cell Biology 2020Quote: ... shLmnA/C(Sigma clone NM_001002011.2-901s21c ...
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bioRxiv - Cell Biology 2022Quote: ... c-Src (Millipore Merck ...
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bioRxiv - Developmental Biology 2019Quote: ... mitomycin-c (Sigma) treated MEFs were seeded on 6-well plates coated with 0.2% gelatin (Sigma) ...
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bioRxiv - Microbiology 2022Quote: Mitomycin C (Sigma) was used to induce the putative antibacterial proteins as outlined by D ...
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bioRxiv - Molecular Biology 2023Quote: ... were purchased from Sigma (C-12302, Sigma). Cells were cultured in EMEM (ECB2071L ...
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bioRxiv - Immunology 2022Quote: ... fumitremorgin C (Sigma), were dissolved at 10 mg/mL in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2023Quote: ... Cytochrome c (Sigma) was added to a final concentration of 150 µg/ml for better visualization of the protein pellet ...
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bioRxiv - Neuroscience 2023Quote: ... fumitremorgin C (Sigma), and ceefourin 1 (Tocris Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... Compound C (Millipore) [8uM].
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bioRxiv - Cell Biology 2019Quote: ... (TDP43: 5’-gcaaagccaagaugagccu-3’ and EGFP control: 5’-gcaccaucuucuucaagga-3’; Sigma). Silenced cells were collected by trypsinization ...
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bioRxiv - Cancer Biology 2020Quote: ... The antibody reaction was visualized with 3-3’ diaminobenzidine (Sigma, D8001) followed by counterstaining with hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... prior to being rinsed and reacted using 3-3’diaminobenzidine (Sigma) as chromagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimidehydrochloride (EDC) were procured from Sigma Aldrich, USA.
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bioRxiv - Physiology 2019Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma-Aldrich, No. E7750) and an amide ...
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bioRxiv - Immunology 2019Quote: ... 3 ml of 3% bacteria-free thioglycollate solution (Sigma, B2551-500G) was injected into the abdominal cavity of C57/BL6 mice ...
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bioRxiv - Plant Biology 2022Quote: ... and crosslinked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma). Biotin-labelled probes were hybridized with sRNAs on the nylon membrane and stabilized streptavidin-horseradish peroxidase was used to detect the biotin signal.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2019Quote: ... the drug compounds (fluoxetine: Sigma-Aldrich, CAS No. 56296-78-7; ketamine: Sigma-Aldrich, CAS No. 1867-66-9; cycloserine: Sigma-Aldrich, CAS No. 68-41-7) were diluted in DMSO and applied in the setup to reach a final concentration of 20μM for each compound ...
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bioRxiv - Molecular Biology 2024Quote: ... 2b–f 3b,c and 4b,c and Extended Data Fig. 1b,c, 2a,c,d, 3a and 4d,e; Sigma-Aldrich, Extended Data Fig. 2b), and also supplemented with penicillin/streptomycin (P/S ...
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bioRxiv - Neuroscience 2021Quote: ... for 3 min and then placed in a solution of 1% w/v Alcian blue in 3% acetic acid (pH 2.5) for 30 min (EMD Millipore, Cat. No. TMS-010-C). Slides were then rinsed in 3% acetic acid and allowed to dry overnight ...