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Citations for Millipore Sigma :
1651 - 1700 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Molecular Biology 2020Quote: ... then diluted to 1×107 cells/ml in liquid YPAD and incubated at 30°C for 2 hours before crosslinking with 1% formaldehyde (Sigma) for 15 minutes followed by quenching with 125 mM glycine for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in PHEM buffer preheated to 37° C (PHEM buffer: 60mM PIPES, 25mM HEPES, 5mM EGTA, 2mM Mg (CH3CO) 2 (Sigma), pH6.9) ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Vitamin C) (Sigma). NSCs were cultured in NSC complete medium consisting of DMEM/F-12 Media ...
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bioRxiv - Systems Biology 2022Quote: ... 3- Supernatant was discarded and 2 ml of prewarmed (35 °C) collagenase-trypsin solution (0.55 mg/ml collagenase, 0.37 mg/ml Trypsin (Sigma, cat. # T0303), and 60 U/ml TurboNuclease in Hank’s balanced salt solution ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL aliquots were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... supernatant was collected in clean microcentrifuge tube and incubated 2 h at 4°C with a-flag mouse antibody (Sigma), following 1 h incubation at 4°C with PureProteomeProtein A/G Magnetic Beads Mix (Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then fixed in 0.1M Na+-cacodylate buffer (pH 7.35, prewarmed to 37°C) containing 2% formaldehyde and 0.1% glutaraldehyde (Sigma-Aldrich, Buchs, Switzerland) for 1 h prior light microscopy imaging ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% TritonX/PBS) for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I ...
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bioRxiv - Microbiology 2021Quote: ... Colicins B1-341 GFP/mCherry were expressed with a 6xHis tail at the C’ terminus and cloned into the multiple cloning site 2 of a pACYCDuet-1 (Novagen) plasmid where they were expressed under a T7 promotor ...
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bioRxiv - Molecular Biology 2022Quote: ... Cross-links were reversed for ChIPs and inputs at 65⁰C overnight with 200 mM NaCl and 2 μl RNase A (Sigma). Samples were then incubated with 20 μg Proteinase K for 1 hr at 45⁰C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: RAW264.7 cells were loaded with 100 μg/ml AcLDL for 16 h at 37 °C with or without 2 μg/ml ACATi (Sandoz 53-035, Sigma) treatment for 2h ...
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bioRxiv - Cancer Biology 2019Quote: ... and digested at 37°C in Hank’s balanced salt solution containing 2 mg/ml type V collagenase (Sigma-Aldrich, C9263) for 30 min under constant agitation ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... membranes were blocked for 1 h at RT in 5% skimmed milk powder and 0.2% Tween in PBS before incubating overnight at 4◦C with the primary antibodies mouse anti-TH (1:1000, Millipore) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... used for barrier dysfunction experiments were conducted on a 37 °C heating block using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M NaHCO3) and cross-linking was reversed overnight at 65°C with 200 mM NaCl and 2 μl RNase A (Sigma). Matched input samples (10% of original ChIP reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained at 37°C in 5% CO2 atmosphere and treated with 2 µg/ml tunicamycin (Sigma-Aldrich), 10 nM-1µM thapsigargin (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... Fixed plant material was digested for 0.5 to 1.5 hours at 37° C in an enzyme mixture consisting of 2 % (w/v) cellulase from Aspergillus niger (Sigma C1184), 4 % (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... coli B834(DE3) carrying pET28b(+)-phm7 was cultured at 18 °C for 96 h using the Overnight Express Autoinduction System 2 (Novagen). Cells expressing the DS were harvested by centrifugation and frozen at –80 °C until use.
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were returned to neutral pH and either incubated at 37°C in the presence of lyso-PC or washed with delipidated BSA (2 mg/ml, Sigma) to remove lyso-PC and then maintained at 37°C for 30 min.
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bioRxiv - Genetics 2020Quote: ... and precipitated with antibodies overnight at 4°C followed by incubation for 2 h with protein G Sepharose beads (Millipore). Normal mouse or rabbit IgG (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Cancer Biology 2021Quote: Cells were fixed in 70% ethanol for at least 2 hrs at −20°C and resuspended in a PBS solution containing 35 ug/ml propidium iodide (Sigma) and 100 ug/ml RNAse A (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... Syn7942 cells were incubated at 30°C with 25 mM NaH14CO3 for 2 minutes before permeabilization with 0.03 % (w/v) mixed alkyltrimethylammonium bromide (Sigma-Aldrich) and RuBP (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... subjected to freeze/thaw at -80°C, and sonicated in lysis buffer (1x PBS, 1% CHAPS, 10mM MgCl2, 2 µl benzonase (25 U/µl, Millipore), and protease inhibitor (Thermo Scientific)) ...
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bioRxiv - Immunology 2021Quote: ... tissue pieces remaining after the DTT/EDTA step were chopped and incubated for 40 min at 37°C with Collagenase D (2 mg/ml; Sigma) and DNase (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...
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bioRxiv - Developmental Biology 2022Quote: ... Each sample was incubated with primary antibodies for 1–2 h at room temperature or overnight at 4°C and then with fluorescent-conjugated secondary antibodies and DAPI (Sigma) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... at 58°C for 2 hr post fixation and subsequently immersed in 1% phosphomolybdic acid (Sigma, St Louis, MO, USA) and 0.5% phosphotungstic acid solution (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Streptococcus pyogenes Cas9 wild type protein and the nuclease dead mutant (D10A, H840A) were both recombinantly expressed for 16 hours at 18 °C in Escherichia coli Rosetta 2 (DE3) (Novagen) N-terminally fused to a hexahistidine affinity tag ...
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bioRxiv - Biochemistry 2022Quote: ... and serially diluted in DMEM media and incubated in 24-well plates with a semi-confluent culture of Vero cells (for ZKV samples) for 1 h at 37°C and covered with DMEM 2% fetal bovine serum + 0.8% of methylcellulose (Sigma, M0512) overlay for 4 days at 37°C and 5% CO2 incubator ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were incubated for 1 – 1.5 h at 37°C followed by addition of warm DMEM with 5% fetal bovine serum and 2% carboxymethyl cellulose (CMC; Sigma) to achieve a 1% CMC overlay ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to Ngn2-medium (Table 1) and cytosine b-D-arabinofuranoside (Ara-C) (2 μM; Sigma, C1768) was added once to remove proliferating cells from the culture ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total protein from PC12 cell extracts were incubated over night at 4°C with 2 μg of the phospho- tyrosine 4G10 (Sigma-Alrich/Upstate Cat# 05-321 ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cell Biology 2022Quote: ... All samples with SDS sample buffer were heated at 95°C for 5 minutes and reduced with 5% 2-mercaptoethanol (Sigma) as needed.
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bioRxiv - Molecular Biology 2023Quote: A small fraction (2%) of each release sample was incubated overnight at 37°C with 1 mg/mL Proteinase K (Sigma), 4 mM CaCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...