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Citations for Millipore Sigma :
1851 - 1900 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... glucose (1 g/Kg; Cat num. 50-99-7, Sigma-Aldrich) was administered by IP injection and blood glucose measured by glucometer as above at 15- ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with wash buffer (10 % formamide (Sigma-Aldrich, 75-12-7) in 2× saline sodium citrate (SSC ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Cell Biology 2022Quote: Cytochrome c (C 2506) was purchased from Sigma Aldrich (Poland).
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bioRxiv - Cell Biology 2022Quote: Cytochrome c (C 2506) was purchased from Sigma Aldrich (Poland).
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bioRxiv - Molecular Biology 2022Quote: ... 0.09 mM cytochrome c from equine heart (Sigma C-7752), and 3U/ml catalase in 5 mM phosphate buffer ...
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bioRxiv - Cell Biology 2020Quote: ... or human NUP153-specific shRNA lentivirus particles overnight at 37°C followed by selection in medium containing Puromycin (Puro, 2 μg/ml) (Sigma-Aldrich) for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated in a humidified atmosphere of 5% CO2 in air at 37°C for 2 hours in a 200 μL drop of TYH medium covered with paraffin oil (Sigma-Aldrich) for sperm capacitation ...
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bioRxiv - Microbiology 2021Quote: Drosophila Schneider line 2 (S2) cells were maintained and passaged at 25°C in Shields and Sang M3 insect medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum.
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bioRxiv - Molecular Biology 2022Quote: ... The explants were then digested for 2 hr at 37°C with 0.01 units chondroitinase ABC (Sigma Aldrich, St. Louis, MO). Samples were then dialyzed with ultrapure water for 24 hr at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37 °C for 30 minutes and then with an equal volume of 2 x Pronase solution (Sigma-Aldrich, Cat. # 10165921001) for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 °C and suspended in resuspension buffer (50 mM Tris-HCl, pH 8, 300 mM NaCl, 2 mg/ml lysozyme (Sigma, L6876), 1 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... and incubated at 37 °C for 2 mins before the addition of D-ribulose 1,5-bisphosphate sodium salt hydrate (RuBP, Sigma Aldrich, US) final concentration 0.04 mM ...
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bioRxiv - Cell Biology 2019Quote: ... muscles were incubated for 60min at 37 °C in the presence of external Tyrode containing 2 mg.mL collagenase (Sigma, type 1). Single fibers were obtained by triturating the collagenase-treated muscles within the experimental chamber.
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bioRxiv - Neuroscience 2019Quote: ... cells at 14 DIV were treated with culture medium containing 100 µg/ml L-ascorbic acid 2-phosphate (vitamin C, Sigma, 49752) for 24 h ...
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bioRxiv - Molecular Biology 2019Quote: ... the slides were then incubated overnight at 4°C with rabbit polyclonal to elafin in 2% BSA at 1:100 dilution (Sigma, HPA017737). The signal was detected using the rabbit horseradish peroxidase EnVision kit (DAKOCytomation ...
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bioRxiv - Cell Biology 2019Quote: ... Medium was removed and cells were incubated for another hour (total infection time: 2 h) at 37 °C in FBS-containing culture medium supplemented with 20 μg/mL gentamicin sulfate (Sigma-Aldrich), to kill extracellular bacteria ...
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bioRxiv - Neuroscience 2020Quote: ... 16 μm) were incubated for 2 h at 30°C with Mouse anti-TH antibody (1:1000, MAB318, Millipore, Burlington, MA) in DEPC-treated phosphate buffer (PB ...
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bioRxiv - Biochemistry 2021Quote: ... at a flow rate of 10 µL/min for 4 min in an isocratic mode of Mobile Phase C (2% acetonitrile (Sigma-Aldrich), 0.1% formic acid) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 107 PFU per ml of MNV-1.CW3 were incubated at temperatures ranging between 0 and 56°C for 2 or 6 h in the presence or absence of 500 μM bile acid (sodium glycochenodeoxycholate GCDCA, Sigma Aldrich) by enumeration by plaque assay.
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bioRxiv - Cell Biology 2021Quote: ... Membranes of primary motor neuron samples were incubated overnight at 4°C with primary antibodies for ERK1/2 (1:10000, Sigma Aldrich) and ATPB (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were cultured at 37°C/5% CO2 and glial growth was inhibited at DIV4 by addition of 5-fluoro-2’-deoxyuridine (30 μM)(Sigma, F0503). A 50% fresh culture medium change was performed every 4 days.
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bioRxiv - Physiology 2020Quote: ... The tube was centrifuged (340 × g for 5 min at 24°C) and the supernatant was seeded on to 2 separate pre-gelatinised (0.2% in dH2O; Type A, Sigma-Aldrich, UK) T25 flasks (Nunc™ ...
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bioRxiv - Biochemistry 2021Quote: MDA-MB-231 cells were lysed with RIPA buffer for 2 h at 4°C and tissues were disrupted and lysed in RIPA buffer (Sigma-Aldrich) using a MagNA Lyser instrument (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... Disulfide bonds were reduced by incubation for 45 min at 57 °C with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Catalog no C4706, Sigma Aldrich). The gels were washed and a final concentration of 20 mM iodoacetamide (Catalog no I6125 ...
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bioRxiv - Plant Biology 2020Quote: ... Supernatant protein concentrations were adjusted to 5 mg/mL and incubated for 2 h at 4 °C with 200 μL of anti-FLAG matrix (Sigma-Aldrich) for co-immunoprecipitation with FLAG-RBOHD and with 200 μL of anti-HA magnetic beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Two-fold serial dilutions of an initial concentration of 5 μg/ml of the mAbs were incubated with NDV LaSota or rNDV-H5 for 3h at 37°C in complete medium supplemented with 50 ng/ml L-1-tosylamido-2-phenylethylchloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Disulfide bonds were reduced by incubation for 45 min at 57°C with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Catalog no C4706, Sigma Aldrich). A final concentration of 20 mM iodoacetamide (Catalog no I6125 ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCS were treated with c-Myc Inhibitor CAS 403811-55-2–Calbiochem (10058-F4) (Millipore Sigma, 475956) at 10 μM or EZh2 inhibitor Tazemetostat (EPZ-6438 ...
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bioRxiv - Microbiology 2022Quote: Yp and Ye were cultured to stationary phase at 28°C and 250 rpm shaking for 16 hours in 2xYT broth supplemented with 2 μg/ml triclosan (Millipore Sigma). Mice of either sex between 8-12 weeks of age were fasted for 16 hours and subsequently inoculated by oral gavage with 100-200 μl phosphate-buffered saline (PBS) ...
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bioRxiv - Pathology 2022Quote: ... 1 part NTA), incubated (30min., RT) and centrifuged (3000g, 1h, 4°C) in an Ultra-2 Centrifugal Filter Unite (Millipore, #UFC203024). Filtered samples (100µL ...
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bioRxiv - Cell Biology 2022Quote: ... Disulfide bonds were reduced by incubation for 45 min at 57 °C with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (Catalog no C4706, Sigma Aldrich). A final concentration of 20 mM iodoacetamide (Catalog no I6125 ...
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bioRxiv - Biophysics 2022Quote: ... Disulfide bonds were reduced by incubation for 45 min at 57 °C with a final concentration of 10 mM Tris (2-carboxyethyl) phosphine hydrochloride (#C4706, Sigma Aldrich). A final concentration of 20 mM iodoacetamide (#I6125 ...
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bioRxiv - Neuroscience 2021Quote: ... and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:200 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... We grew donor strains in 96-well deep-well plates (1-mL cultures in TY medium in 2-mL wells) for at least 5 h shaking at 37 °C with a Breath-easy (Sigma-Aldrich) film covering the plate ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was removed and the cell pellet was resuspended in 500 μL Diluent C containing 2 μL PKH67 dye (PKH67 Green Fluorescent Cell Linker Kit, Sigma-Aldrich), incubated for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of these extracts were centrifuged at 14,000 xg for 1 min at 4°C with 2.5 μl 2% (v/v) Tween 20 (Sigma-Aldrich, Darmstadt, Germany) to promote the aggregation into a pellet ...
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bioRxiv - Biochemistry 2021Quote: ... in a pre-cooled MLA-80 rotor at 2°C for 30 minutes and then filtered through a 0.45-μm Milliex-HV polyvinylidene fluoride membrane (Millipore, Bedford, MA). The filtrate was loaded onto an equilibrated TOG-column ...
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bioRxiv - Cancer Biology 2022Quote: ... and 100 mM 2-deoxy-D-glucose (Port C) were sequentially injected in 25 μL volume (all reagents from Sigma-Aldrich). For measuring the metabolic activity after M2d or MS-5 co-culture ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: Non-synchronized and synchronized cells were fixed using 70% Ethanol for 48 hours at 4°C then washed with PBS+2% goat serum (Sigma, UK) and resuspended in 1mL of ice-cold PBS ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl and 0.1% Tween-20 or TBST buffer, 2 h) and incubated (4°C, overnight) with biotinylated BSI-B4 lectin from Bandeiraea (Griffonia) simplicifolia (Sigma-Aldrich, #L3759-1MG ...
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bioRxiv - Cell Biology 2020Quote: ... the wafer was baked at 95°C for 2 min and cooled to room temperature and developed in a propylene glycol monomethyl ether acetate (Sigma Aldrich) bath ...
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bioRxiv - Microbiology 2019Quote: The extracted lipids were concentrated to dryness under nitrogen stream and then methylated by the presence of methanol and sulphuric acid (5% v/v) for 2 h at 85°C in the presence of 50 μg heptanoic acid (Sigma Aldrich) as internal standard ...
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bioRxiv - Physiology 2021Quote: ... Then centrifuged for 5 minutes at 800 g at 5°C and the pellet was resuspended in 500 μl sorting buffer (1 % BSA wt/vol, 2 % skimmed milk powder (Sigma Aldrich), 1 mM EDTA in PBS pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 0.1 % Triton X-100 and incubated overnight at 4 °C or for 2 h at room temperature with the following primary antibodies: anti-REST (1:500; 07-579 Millipore, MA), anti-Npas4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... were cleaned by incubation at 56°C overnight in 2% Hellmanex III (Hellma Analytics) dissolved in ultrapure Milli-Q® water (Millipore) followed by 30 minutes sonication in a heated sonication bath ...