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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for 7 Chlorothieno 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... sodium dodecyl phosphate (SDS) 0.1%, 25mM Tris) supplemented with protease and phosphates inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3) (Sigma-Aldrich) and containing 4% β-mercaptoethanol ...
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bioRxiv - Developmental Biology 2021Quote: Primary polyps were incubated in freshly filtered (0.2 μm) seawater with 3 μM calcein blue (Sigma–Aldrich 54375-47-2) for 4-5 hours ...
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bioRxiv - Immunology 2020Quote: ... 8-20 weeks after reconstitution chimeric mice were injected intraperitoneally for 3 d with 2 mg/day of tamoxifen (Sigma) re-suspended at 20 mg/ml in corn oil (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Slices were transferred to Millicell-CM membrane inserts (0.4 μm pore size, 30 mm diameter, 2-3 slices per insert; Millipore Corp) in six well tissue culture treated plates containing MEM with 25% horse serum and 6.5 mg/mL of glucose ...
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bioRxiv - Microbiology 2021Quote: ... harvesting at 3 dpi) using DMEM supplemented with 2% FBS and 1 μg/mL TPCK-trypsin (Sigma-Aldrich #1426-100MG). SARS-CoV and MERS-CoV viral stocks were generated by infecting VeroE6 cells (MOI 0.0001 ...
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bioRxiv - Cell Biology 2021Quote: Confluent ESCs monolayers were decidualized in DMEM/F12 containing 2 % FBS supplemented with 0.3 mM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP) (Sigma-Aldrich, USA), 10 nM β-Estradiol (E2 ...
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bioRxiv - Immunology 2021Quote: ... were pelleted at 3.000g for 3 minutes and induced by resuspension in YNB-CAA + 2% D-(+)-Galactose (Sigma G0625-500G) + phosphate buffer + P/S induction medium at a final OD600 of 0.5 ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were kept at dim light (2-3 μE) for 16 h and plated on fresh TAP plates containing 10 μg/mL Paramomycin (Sigma). Plates were incubated for 10 d at 30-50 μE constant light ...
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bioRxiv - Bioengineering 2022Quote: ... with a 3:1 M ratio of 5-norbornene-2-carboxylic acid (mixture of endo and exo isomers; Millipore-Sigma) to HA-TBA repeat units ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 500 μL 2% Alizarin Red solution (Sigma-Aldrich, 05600) diluted in UPW for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 0.1 % Tween-20 and 2 mM EDTA before incubating in a blocking solution (3 % acetylated bovine serum albumin (Sigma, MO), 0.1 % Tween-20 ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures were grown in a mixture of 2:3 ratio of Todd-Hewitt media with 0.5% yeast extract (Sigma-Aldrich), and Brain-Heart Infusion media (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... media was exchanged for 2 mL of culture media supplemented with 500 µM 3-indoleacetic acid (IAA, Millipore Sigma, I2886). Each dish was transfected with 400 ng of the reporter plasmid using X-tremeGENE HP DNA transfection reagent (Roche ...
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bioRxiv - Molecular Biology 2023Quote: Cytotoxicity of Quercetin and other natural compounds was evaluated using MTT (3-(4, 5- dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye) assay (Sigma Aldrich). Following the drug treatment in triplicate at indicated concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... Proliferative capability of BMECs was tested by 3-[4,5-dimethylthiazyol-2-yl] -2,5-diphenyltetrazolium bromide (MTT, 5mg/ml, Sigma, cat# CT01) assay ...
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bioRxiv - Cell Biology 2022Quote: ... cells were synchronised in early S phase by two consecutive rounds of treatment with 2 mM thymidine and 3 ug/ml (Sigma) aphidicolin ...
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bioRxiv - Genetics 2023Quote: ... animals of similar age were depleted of AID::GFP expression by culturing for 2 h on NGM plates containing 100 µM auxin (3-indoleacetic acid; Sigma). Directly after depletion ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions (some of which were pooled from tumors harvested from 2-3 mice) were stimulated for 5 h with PMA (5 ng/ml, Sigma) and ionomycin (500 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Penis and lymph nodes were dissected and fixed for 1.5-2 h in 3% paraformaldehyde (penis) or overnight in 1% paraformaldehyde (PFA, Sigma, P6148) at 4°C (lymph nodes ...
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bioRxiv - Immunology 2023Quote: ... for 1-2 hours at RT followed by 3 washes in PBS + 0.1% Tween 20 (PBS-T; Sigma P7949-100ML). Primary antibody was then fixed to the tissue for 30 minutes in 4% PFA for 30 minutes at RT followed by 3 washes with PBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... 3 µL of 2 M CaCl2 and 5 µL of 10 mg/mL ε-aminocaproic acid (ε-ACA) (Sigma-Aldrich), and 1 µL of 1U/µL Thrombin (MP Biomedicals ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were also washed for 10 minutes in a solution of 1.25×10−3 mg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Sigma: D9542) in PBS to fluorescently label nuclei within tissue sections.
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bioRxiv - Cell Biology 2023Quote: ... composed of Egg yolk L-α-Phosphatidylcholine (EyPC, Avanti Polar), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, Avanti Polar) and Cholesterol (Sigma) (50:20:30 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were inoculated in YEP containing 3% lactic acid and treated with a final concentration of 2% galactose (Sigma-Aldrich). For the inducible degradation of the proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Then the brains were dissected to 2 mm slices and incubated for 10 minutes in freshly prepared 0.5% TTC solution (2, 3, 5-Triphenyltetrazolium chloride, Sigma-Aldrich). Afterward ...
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bioRxiv - Cancer Biology 2023Quote: ... two to four-month-old mice were gavaged 3 times over 5 days with 2 mg tamoxifen (TX; Sigma #T5648) in corn oil (Sigma #C8267) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mm3 tissue pieces from 2-3 brains were pooled and enzymatically digested in a dissociation medium containing 0.16 mg/mL cysteine (Sigma C9768), 7 U/mL Papain (Sigma P3125) ...
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bioRxiv - Developmental Biology 2023Quote: ... lentiviruses were generated by transfecting 3 x 106 HEK-293T cells in antibiotic-free medium with 6 µg of total DNA in a 1:2:3 ratio of pMD2.G:psPAX2:transfer plasmid using polyethylenimine (PEI) (Sigma-Aldrich). 48 h after transfection ...
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bioRxiv - Systems Biology 2024Quote: ... 2M thiourea, 100mM Tris-HCl, pH 8, 150mM NaCl, 1mM EDTA, phosphatase inhibitor cocktail 2 and 3, 10mM NaF; Sigma), reduced with 10mM DTT (dithiothreitol ...
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bioRxiv - Neuroscience 2024Quote: ... and 2-(14-carboxytetradecyl)-2-ethyl-4,4-di methyl-3-oxazolidinyloxy (16-doxyl-stearicacid, 16-DSA, cat. no. 253596) from Sigma-Aldrich. 5-DSA (spin probe containing the nitroxide group attached on C5 that is located on the opposite terminal relative to the charged carboxyl fatty acid terminus ...
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bioRxiv - Immunology 2023Quote: ... The activity of trypsin was inhibited by dropping the pH to 2-3 by the addition of 10% trifluoroacetic acid (TFA, Sigma). The samples were loaded on Evosep tips to separate the digested peptides using nanoflow reversed-phase chromatography with an Evosep One liquid chromatography (LC ...
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bioRxiv - Cell Biology 2023Quote: ... (phenylmethylsulfonyl fluoride, PMSF 1mM; 1-chloro-3-tosylamido-4-phenyl-2-butanone, TPCK, 10 μg/ml; aprotinin, 10 μg/ml Sigma) incubated for 30min on ice ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated for 30 minutes with 3 µmol/L 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma-Aldrich, D225). After that ...
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bioRxiv - Neuroscience 2024Quote: ... Flies were housed singly for 2–3 days after the procedure and were subsequently head-fixed to a custom mount with eicosane (Sigma) and imaged on a Movable Objective Microscope with resonant scanners (MOM ...
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bioRxiv - Bioengineering 2023Quote: ... tricaine-anesthetized 3 or 4 days-post fertilization (dpf) larvae were mounted in 2% low-melting point agarose (Sigma-Aldrich) on a 35 mm glass bottom dish (#1.5H Glass ...
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bioRxiv - Genetics 2024Quote: ... differentiation of iPSCs was induced through the Wnt modulation method with 7 μM glycogen synthase kinase 3 b (GSK3b) inhibitor CHIR99021 (Selleckchem) and 5 μM inhibitor of WNT production 2 (IWP2) (Sigma). Basal Media was composed of RPMI media supplemented with B27 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: Auxin treatments were performed by immersing dissected SAM in 1 or 2 mM Indole-3-acetic acid (IAA) solution (Sigma) for five hours as described (Galvan-Ampudia et al. ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing PelXC232S were pooled and concentrated to a volume of 2 mL by centrifugation at 2 200 × g at a temperature of 4 °C using an Amicon ultra centrifugal filter device (Millipore) with a 10 kDa molecular weight cut-off ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitations were performed on 2 mg of total protein by incubating 2h at 4°C with anti-Flag agarose beads (Sigma). Beads were then washed in lysis buffer and bound polypeptides analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M NaHCO3) and cross-linking was reversed overnight at 65°C with 200 mM NaCl and 2 μL RNase A (Sigma). A matched input sample (1/10 of original ChIP reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 M NaHCO3) and cross-links were reversed overnight at 65°C with 200 mM NaCl and 2 μl RNase A (Sigma). A matched input sample (10% of original ChIP reaction ...
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bioRxiv - Genomics 2019Quote: ... The reaction was carried out at 60°C for 2 hours in a custom labeling buffer with a final concentration of 50 mM K-acetate (Sigma), 10 mM Mg-Acetate (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were subsequently incubated in blocking buffer containing 0.1% Tween 20 and the appropriate primary antibodies for 1-2 hr at room temperature or 4°C overnight: rabbit anti-Myc (1:2,000, Sigma, C3956), mouse anti-FLAG (1:2,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse CTCF cDNA corresponding to the full-length 736 amino acids was cloned with C-terminal 6xHis tag into pFLAG-2 (Sigma). FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Biophysics 2020Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Virus containing fractions were collected ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...