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Citations for Millipore Sigma :
1451 - 1500 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... using primers designed with SnapGene® (version 6.1.1) below and produced by Sigma Aldrich (see Supplemental Fig 3B for each). Reactions were analysed with ViiA 7 Real-Time PCR System (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... the underlying gene fragments were amplified by PCR using the primer pairs LC195/LC196 for both versions of YopR and LC192/193 for YosL and introduced into a pET24d (Novagen) derivative modified for modular cloning via BsaI restriction sites ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Genetics 2020Quote: ... and 1 μl of 1:10 diluted DNA was used as a template for PCR using the REDTaq ReadyMix PCR Reaction Mix (Sigma-Aldrich). The PCR conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... 5’-CTGGGAGTATACTTGTAGTC-3) were run in multiplex with PCR reaction mixtures consisting of 10 μl redtaq (REDTaq ReadyMix PCR Reaction Mix, Sigma-Aldrich), 2 μl DNA ...
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bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
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bioRxiv - Microbiology 2020Quote: ... the junctions of targeted integration were amplified by (semi-)nested PCR using 300 ng genomic DNA containing 1x PCR buffer (Sigma Aldrich), 1.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2019Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Genomics 2021Quote: ... The number of necessary library PCR amplification cycles was determined by qRT-PCR using SYBR Green 10x in DMSO (Sigma-Aldrich) and NEBNext Master Mix 2x (New England BioLabs) ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA substrate was generated by PCR and the products were purified using a GenElute PCR Clean-up kit (Sigma-Aldrich). If required ...
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bioRxiv - Developmental Biology 2021Quote: ... The modern DNA of each species was used for a long-range PCR (Sigma Aldrich Expand™ Long Template PCR System). Two primer pairs were used for the human mtDNA amplification (Gunnarsdóttir et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as previously described (41,44) ...
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bioRxiv - Developmental Biology 2020Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Sigma-Aldrich) as described previously (Rumi et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Genotypes were verified by PCR of tail DNA using the RedExtract-N-Amp Tissue PCR Kit (Sigma Aldrich 254-457-8).
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bioRxiv - Neuroscience 2023Quote: ... All cells were regularly tested for the presence of mycoplasma using the PCR Mycoplasm Test Kit (PromoKine) or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Immunology 2023Quote: ... a targeted amplicon library was generated by PCR from the standard gene expression library cDNA using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004). 50uL PCR reactions were prepared by combining 25uL ReadyMix with 2.5 uL of a 10uM stock of each primer (10x Partial Read 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Genetics 2022Quote: Genomic regions of interest were amplified by PCR (Phusion™ High-Fidelity DNA Polymerase, Thermo Scientific or KAPA2G Robust HotStart PCR, Sigma) from genomic DNA samples ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µl of locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and TruSeq i5 and i7 indexing primers (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The bacterial solution was then incubated at room temperature (RT) for 20 min under moderate shaking in the presence of 0.5 mg/mL lysozyme (Sigma-Aldrich) at and additional 10 min after addition of 0.1 mg/mL DNase ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS for 30 min before incubation with primary antibodies for 1 h at RT in the permeabilization buffer supplemented with 0.5% normal goat serum: TFE3 (0.6 μg/ml; Sigma-Aldrich, HPA023881), LAMP1 (1/50 conditioned medium of hybridoma cells ...
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bioRxiv - Immunology 2021Quote: ... The H1568 cells were then fixed in 4% formaldehyde solution in PBS for 30 min at RT and then permeabilized with 0.2% (v/v) Triton X-100 (Sigma-Aldrich) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally detached from the mask and incubated for 1 hour at RT with a 5 μg/mL solution of fibronectin (Sigma) in NaHCO3 100 mM ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain sections were dried for 20 minutes at RT and mounted on 0.1% gelatin-coated slides with FluoroshieldTM with DAPI (Sigma-Aldrich). Sections were visualized and photographed using a Zeiss Axio Imager Z1 microscope equipped with an Axiocam MR3.0 camera and Axivision 4.9.1 software ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was split in half and incubated for 1h at RT (while rotating) with either anti-FLAG M2 affinity gel (Sigma) or anti-HA affinity matrix (mAb 3F10 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed and incubated in CCF4 for 1 h at RT in the presence of 2.5 mM probenecid (Sigma-Aldrich). Live cells (propidium-iodide negative ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... 4% in PBS for 15 min at RT] and subjected to staining with filipin (40 μg/mL in PBS for 2 h at RT; from 250-fold ethanolic stock solution; F9765, Sigma). Fluorescence images of cultured cells were acquired using an inverted microscope (Axiovert 135TV ...
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bioRxiv - Immunology 2021Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of pre-reverse transcription (RT) lysis mix containing 0.1 μl of NP40 (Sigma-Aldrich), 0.5 μl of RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS with 0.05% Tween-20 for 1 h at RT before addition of primary antibodies (SRRM4, HPA052783, Sigma-Aldrich Quimica S.A. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 14 days of differentiation with 4% paraformaldehyde in PBS for 10 min at room temperature (RT) and immunofluorescence staining was performed with βIII-tubulin (1:5,000 dilution, Sigma, T8660), NF200 (1:6,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at RT and then stained with a primary antibody (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... in Tris-buffered saline (TBS) for 15 min at RT and then further treated with 0.1% Triton-X-100 (Sigma-Aldrich) and 2% goat serum in TBS overnight at 4°C for improving antibody penetration ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... PBST) for 1 h at RT followed by incubating with primary antibodies (anti-PV, 1:1000-2000, P3088, Sigma-Aldrich) in 3% NGS in PBST at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were treated with a 10 min RT incubation in 0.1% Triton-X 100 (Sigma, St. Louis, MO, Cat. # X100) to permeabilize cells ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were then permeabilized with 0.1% Triton X-100 for 15 min at RT and blocked with 1% bovine serum albumin and 5% goat serum (Sigma). cells were incubated with primary antibodies Esrrb (PP-H6705-00 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were grown to near confluency on 6 cm dishes and total RNA was extracted using the RNAzol RT reagent (Sigma) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Cells grown on coverslips (1.5#) were incubated for 20 minutes at room temperate (RT) in PBS with 4% paraformaldehyde (Sigma-Aldrich) and washed three time for 10 minutes with PBS ...
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bioRxiv - Genomics 2022Quote: ... the slides were incubated in 1% Kodak PhotoFlo at RT for 1 minute and counterstained with 0.5 mg/mL DAPI (Sigma–Aldrich) in antifade DABCO (Sigma–Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Germany) slides were incubated for 1h at room temperature (RT) in TBS containing 10% donkey serum (Sigma-Aldrich, Vienna, Austria), 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of a pre-reverse transcription (RT) lysis mix containing 0.1 μl NP40 (Sigma-Aldrich), 0.5 μl RNAse Inhibitor (Thermo Fisher) ...