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Citations for Millipore Sigma :
1551 - 1600 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Routine genotype analysis of mice was performed by PCR assay on DNA purified from tail biopsies (Extract N-Amp Tissue PCR kit, Sigma Aldrich). Wild-type and KO mutant alleles were distinguished using primers available on request ...
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bioRxiv - Cell Biology 2024Quote: ... or 12-well (1x105 cells/well) plates for western blotting and quantitative real-time PCR (qRT-PCR) experiments or on 0.2% gelatin (Sigma Aldrich, G1890)-covered 96-well plate (Grainer Bio-One ...
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bioRxiv - Genetics 2022Quote: Genomic regions of interest were amplified by PCR (Phusion™ High-Fidelity DNA Polymerase, Thermo Scientific or KAPA2G Robust HotStart PCR, Sigma) from genomic DNA samples ...
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bioRxiv - Biochemistry 2024Quote: ... A total of 1 µl of locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase (EMD-Millipore, 71086-31) and TruSeq i5 and i7 indexing primers (Illumina ...
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bioRxiv - Cell Biology 2019Quote: PCR products were ligated into the p3XFLAGCMV10 (Sigma-Aldrich) and pEGFP-C1 (Clontech ...
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bioRxiv - Cell Biology 2020Quote: ... PCR-amplified DNA fragments were inserted into pET21b (Novagen) without adding a tag using NheI and XhoI restriction sites ...
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bioRxiv - Genomics 2019Quote: ... and 1 µl of 10× PCR Buffer (Sigma Aldrich) with 0.25 units of JumpStart Taq DNA Polymerase (Sigma Aldrich ...
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bioRxiv - Genetics 2019Quote: ... in Multiscreen PCR cleaning plates (Millipore, Burlington, Massachusetts, USA) with vacuum application (10 psi) ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were prepared with 1.0M Betaine (Sigma-Aldrich), and were heated to 80°C before adding Taq Polymerase (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was ligated into pET28a(+) plasmid (Novagen), which was linearized with Nhe I and BamH I ...
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bioRxiv - Cancer Biology 2020Quote: ... Regular Mycoplasma testing was performed using MycoLookOut PCR (Sigma). OVSAHO ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned in the pET28b vector (Novagen) linearized with BamHI and NotI according to the sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Cell Biology 2021Quote: ... Oligonucleotides for PCR amplification were obtained from Sigma Aldrich. For each construct the relevant open reading frame was completely verified by Sanger sequencing (GATC/Eurofins Scientific) ...
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bioRxiv - Microbiology 2022Quote: Expand High Fidelity PCR System (Sigma-Aldrich, catalog #: 11759078001).
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bioRxiv - Cancer Biology 2022Quote: ... confirmed by the use of Mycoplasma PCR Detection (Sigma) were regularly passaged to maintain exponential growth and used from early passages (<10 passages after thawing) ...
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bioRxiv - Cancer Biology 2020Quote: ... or the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... cerevisiae DNA by PCR and cloned into pET21b (Novagen) via InFusion cloning (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... Amplicons were purified using a PCR cleanup kit (Sigma) and quantified using a Qubit 2.0 fluorometer (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The purified PCR product and pET-28a(+) (Novagen, Germany) were digested with BamH I and Xho I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were then cloned in the pET15b (Novagen) plasmid modified compatible with GatewayTM cloning strategy ...
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bioRxiv - Cancer Biology 2022Quote: ... Regular mycoplasma testing was performed using MycoLookOut PCR (Sigma). When were they last tested? HGSOC lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... using RedExtract-n-Amp tissue PCR kit (Sigma-Aldrich). The G2 progeny of genome-edited males were then raised until homozygous stocks were established ...
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Microbiology 2023Quote: ... PCR products and the pET17b plasmid (Novagen (EMD Millipore), cat ...
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bioRxiv - Cell Biology 2024Quote: ... All PCR was performed using KOD polymerase (EMD Millipore). To generate pPM09 ...
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bioRxiv - Microbiology 2023Quote: ... Colony PCR was performed using REDtaq Ready Mix (Sigma) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... were amplified by PCR using KOD Xtreme polymerase (Novagen) using primers (Table S5 ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products were run in 2% agarose (SIGMA) gels and visualised under UV light.
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bioRxiv - Microbiology 2020Quote: ... The bacterial solution was then incubated at room temperature (RT) for 20 min under moderate shaking in the presence of 0.5 mg/mL lysozyme (Sigma-Aldrich) at and additional 10 min after addition of 0.1 mg/mL DNase ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated free-floating overnight at RT with the mouse G10 anti-reelin primary antibody (1:3000, MAB5406; Millipore) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS for 30 min before incubation with primary antibodies for 1 h at RT in the permeabilization buffer supplemented with 0.5% normal goat serum: TFE3 (0.6 μg/ml; Sigma-Aldrich, HPA023881), LAMP1 (1/50 conditioned medium of hybridoma cells ...
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bioRxiv - Immunology 2021Quote: ... The H1568 cells were then fixed in 4% formaldehyde solution in PBS for 30 min at RT and then permeabilized with 0.2% (v/v) Triton X-100 (Sigma-Aldrich) in PBS for 10 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were finally detached from the mask and incubated for 1 hour at RT with a 5 μg/mL solution of fibronectin (Sigma) in NaHCO3 100 mM ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... The brain sections were dried for 20 minutes at RT and mounted on 0.1% gelatin-coated slides with FluoroshieldTM with DAPI (Sigma-Aldrich). Sections were visualized and photographed using a Zeiss Axio Imager Z1 microscope equipped with an Axiocam MR3.0 camera and Axivision 4.9.1 software ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was split in half and incubated for 1h at RT (while rotating) with either anti-FLAG M2 affinity gel (Sigma) or anti-HA affinity matrix (mAb 3F10 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with primary antibodies in BB for 1h at RT: monoclonal mouse anti-Col4a2 (Millipore, Cat. No. MAB1910), polyclonal rabbit anti-LM (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the cells were washed and incubated in CCF4 for 1 h at RT in the presence of 2.5 mM probenecid (Sigma-Aldrich). Live cells (propidium-iodide negative ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoblotting was performed with the following primary antibodies (overnight at 4°C) and secondary antibodies (1h at RT): mouse-anti-Flag (M2 monoclonal, #F3165, 1:3000, Sigma), rabbit-anti-GAPDH (monoclonal ...
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bioRxiv - Neuroscience 2021Quote: ... 4% in PBS for 15 min at RT] and subjected to staining with filipin (40 μg/mL in PBS for 2 h at RT; from 250-fold ethanolic stock solution; F9765, Sigma). Fluorescence images of cultured cells were acquired using an inverted microscope (Axiovert 135TV ...
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bioRxiv - Immunology 2021Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of pre-reverse transcription (RT) lysis mix containing 0.1 μl of NP40 (Sigma-Aldrich), 0.5 μl of RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 5% milk in TBS with 0.05% Tween-20 for 1 h at RT before addition of primary antibodies (SRRM4, HPA052783, Sigma-Aldrich Quimica S.A. ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed after 14 days of differentiation with 4% paraformaldehyde in PBS for 10 min at room temperature (RT) and immunofluorescence staining was performed with βIII-tubulin (1:5,000 dilution, Sigma, T8660), NF200 (1:6,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at RT and then stained with a primary antibody (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then pre-incubated for 1 h at RT in incubation buffer: 5 % normal donkey serum (EMD Millipore, USA) and 0.2 % Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... in Tris-buffered saline (TBS) for 15 min at RT and then further treated with 0.1% Triton-X-100 (Sigma-Aldrich) and 2% goat serum in TBS overnight at 4°C for improving antibody penetration ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... PBST) for 1 h at RT followed by incubating with primary antibodies (anti-PV, 1:1000-2000, P3088, Sigma-Aldrich) in 3% NGS in PBST at 4 °C overnight ...