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Citations for Millipore Sigma :
1401 - 1450 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 (DE3) pLysS cells (Novagen). Cells were grown to an O.D.600 of 0.6 at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... coli expression plasmid pET-15b (Novagen), using these same sites ...
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bioRxiv - Cell Biology 2023Quote: ... coli O55: B5 (Sigma Aldrich, # L6529). Cells were treated with different periods of exposition to LPS (2 ...
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bioRxiv - Biochemistry 2023Quote: ... coli was purchased from Sigma Aldrich [Cat# G5635] α1,2-Fucosidase [Cat# P0724S] and α2,3-Neuraminidase [Cat# P0743] were purchased from New England BioLab ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli B834(DE3) (Novagen, Madison, WI) was induced with 0.25 mM IPTG at 25 °C for 16 h ...
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bioRxiv - Plant Biology 2023Quote: ... coli strain BL21 (DE3) CPRIL (Novagen). Recombinant proteins corresponding to the full-neck regions of myosin VIIIs were solubilized using 8 M urea and purified using a Ni-NTA His60 column (Takara) ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21(DE3) Rosetta™ (Novagen) harboring pSAJ016 (pET28a derivative containing the coding sequence of scab57751 (cebE ...
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bioRxiv - Microbiology 2023Quote: ... coli Rosetta(DE3)pLysS (Novagen, Germany). Cells were cultivated at 37 °C in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains: Rosetta (Novagen, 71402-3), One Shot BL21(DE3 ...
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bioRxiv - Neuroscience 2023Quote: ... coli O111:B4 (Sigma-Aldrich; L2630) to induce fever and sickness ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 (DE3)RP strain (Novagen) for overexpression ...
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bioRxiv - Biophysics 2023Quote: ... coli strain BL21 Gold (DE3) (Novagen) at 37°C and grown in 1-liter cultures in terrific growth media to an optical density at 600 nm of 1.0 ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star (DE3) cells (Novagen) as described ...
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bioRxiv - Immunology 2023Quote: ... coli O111:B4 was from Sigma. TLR2/TLR1 ligand Pam3CSK4 and TLR2/6 ligand FSL-1 were from InvivoGen ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) cells (Sigma-Aldrich) cultured in 2x YT medium supplemented with the antibiotics of ampicillin ...
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bioRxiv - Bioengineering 2023Quote: ... coli BL21 (DE3) cells (Sigma-Aldrich) cultured in 2x YT medium supplemented with the antibiotics of ampicillin ...
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bioRxiv - Biochemistry 2023Quote: ... coli BLR competent cells (Millipore/Novagen). Expression was initiated by the addition of 1 mM IPTG to 1 L of log-phase culture in LB and allowed to proceed for 3 h at 37°⁰C ...
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bioRxiv - Biochemistry 2023Quote: ... coli BLR competent cells (Millipore/Novagen). Expression was initiated by the addition of 1 mM IPTG to 1 L of log-phase culture in LB and allowed to proceed for 3 h at 37°⁰C ...
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bioRxiv - Microbiology 2023Quote: ... coli strain Rosetta2 pLysS (EMD Millipore) by induction with 0.25 mM IPTG at 20°C for 16 hours.
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bioRxiv - Biochemistry 2024Quote: ... coli OverExpress C41(DE3) cells (Sigma). A single colony was selected and grown at 37ºC until the mid-log phase and induced overnight with 1 mM IPTG at 16ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... coli O111:B4 (Sigma-Aldrich L2630) was diluted to 100 ng/mL in experimental media for all experiments.
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bioRxiv - Biophysics 2024Quote: ... coli BL21 (DE3) strain cells (Novagen) using Luria Broth (HiMedia ...
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bioRxiv - Bioengineering 2024Quote: ... coli Rosetta cells (DE3; Novagen, USA) and grown on LB agar plates containing kanamycin (50 µg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... coli Rosetta II (DE3) (Sigma-Aldrich) for recombinant overexpression ...
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bioRxiv - Biochemistry 2024Quote: ... coli Rosetta (DE3) (Novagen, Darmstadt, Germany). The SUMO3 domain located between the tag and the ADF ensured a native N-terminus ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli Rosetta2 (DE3) BL21 derivative (Novagen)
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bioRxiv - Physiology 2024Quote: ... coli O111:B4 (Sigma, catalog #L2630) or with live DH5α Escherichia coli (E ...
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bioRxiv - Neuroscience 2021Quote: ... with spike threshold at 133% above baseline noise (calculated using 3-Sigma Peak Heights detector ...
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bioRxiv - Cancer Biology 2021Quote: ... The matrix solution consisted of 10 mg/ml 9-aminoacridine hydrochloride monohydrate (9-AA) (Sigma-Aldrich, Germany) in water/methanol 30:70 (v/v) ...
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bioRxiv - Neuroscience 2022Quote: ... The upper portions of the membranes were incubated with MAB2166 (recognizing HTT aa 181-810, Millipore, 1;1000) along with an mTOR antibody (Cell Signaling 2972S ...
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bioRxiv - Cell Biology 2023Quote: The Cno DIL domain sequence (aa 613-1006) was cloned into plasmid pET28 (Millipore Sigma, Burlington, MA) using PCR and primers with engineered NheI and EcoRI restriction sites ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Mice received e-cigarette vapor produced from e-liquid containing 70/30 propylene glycol and glycerol with nicotine (Sigma) at a concentration of 6 mg/mL or without nicotine ...
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bioRxiv - Genomics 2020Quote: ... HEK293T and U-2 OS cells were seeded on to poly-D-lysine (Cat. No. A-003-E, Sigma, St. Louis, USA) pre-coated 24-well plates at 24 h prior to transfection.
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bioRxiv - Genomics 2019Quote: ... 2×105 HEK293T cells were plated onto each well of a 25 ug/ml poly-D lysine (EMD Millipore, A-003-E) coated 24-well plate ...
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore, Burlington, Massachusetts,USA), pore size ...
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bioRxiv - Cancer Biology 2019Quote: ... containing 1% glucose, and protease inhibitors (0.1 mM phenylmethanesulfonyl fluoride, 2 μM E-64 and 10μM leupeptin) (Sigma-Aldrich, St. Louis, Missouri) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-alpha-tubulin (Clone B-5-1-2) (1:1000, Sigma, Cat# T5168), and mouse anti-PyCSP (1:1000 ...
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bioRxiv - Physiology 2022Quote: Skeletal muscle sections were staining with anti-laminin-2 (L0663, Sigma, 1:1000 dilution) to outline the muscles fibers ...
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bioRxiv - Physiology 2019Quote: ... Nuclei were stained with DAPI (1:1000; 4’,6-diamidino-2-phenylindole dihydrochloride; Sigma).
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bioRxiv - Developmental Biology 2021Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Sigma #D9542, 1:1000 for 10min at RT) Mice were deeply anesthetized with isoflurane and then transcardially perfused with ice-cold heparinized PBS (25U/ml ...
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bioRxiv - Neuroscience 2022Quote: ... After staining nuclei with DAPI (1:1000, 4’, 6-diamidino-2-phenylindole dihydrochloride; Sigma) and several washes with PBST ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with DAPI (4,6-diamidino-2-phenylindole) (Sigma-Aldrich; #MBD0015; 1:1000) for 5 min at room temperature in the dark (except FUCCI cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1:1000 sheep anti-rabbit F(ab’)2 Cy3 (Sigma-Aldrich, cat. C2306). F-actin and DNA staining were performed by incubating the embryos and larvae in 1:100 BODIPY FL-Phallacidin (Life Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... for 2 hours at room temperature and counterstained with Hoechst (1:1000; Sigma-Aldrich) for 10 minutes prior to imaging ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Plant Biology 2024Quote: 5 μg kinase OsDMI3-GST was incubated with 5 μg substrates (OsPrx20-His, Os Prx20T244A-His, or myelin basic protein [MBP; Sigma-Aldrich]) in kinase reaction solution for 30 min as described previously (Shen et al. ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tag (Sigma, Cat. #F7425, 100 ng/ml), and GRASP55 (Rich ...
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bioRxiv - Molecular Biology 2019Quote: ... HA and flag epitope tags (Roche and Sigma). Immunoreactive proteins were visualized with HRP-labeled secondary antibodies (Pierce ...