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Citations for Millipore Sigma :
1351 - 1400 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... was set up by picking a fungal colony from YPD agar plates (50 g/L YPD broth powder [Sigma-Aldrich], 2% Agar [MP Biomedical]) and resuspending in 3 mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]) ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then centrifuged at 4,000 rpm for 22 min at 4°C and the supernatants were transferred to another set of 6 eppendorf tubes containing a mixture of pre-washed beads (Millipore Sigma, GE17-0886-01) and 12.5 uL (12.5 ug ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and real-time quantitative reverse transcriptase PCR analysis (qRT-PCR) was performed using SYBR Green® reagent (Sigma Aldrich) as previously described (46) ...
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bioRxiv - Genetics 2022Quote: ... Real-time PCRs were done in a 10 μl volume using the Absolute quantitative PCR SYBR Green mix (Sigma) in a 96-well plate ...
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bioRxiv - Genetics 2019Quote: ... 50 uL of PCR product of each sample was purified with SpinPrep PCR Clean-Up Kit (EMD Millipore Corp) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... the tagmented DNA was amplified by PCR using the KAPA2G Robust PCR kit (Sigma-Aldrich, St. Louis, MO, USA) using the GC buffer along with 0.2 μM of each custom P5 and P7 indexing primers (File S1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Template plasmid was digested using DpnI and the PCR products were purified using a PCR purification kit (Sigma-Aldrich). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... The amplified DNA was then purified by depositing the PCR products in a 96-well MultiScreen PCR plate (Millipore). A vacuum of 15 inHg was applied to the wells containing the PCR products until all the wells were dry (12-15 minutes) ...
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bioRxiv - Systems Biology 2020Quote: ... secondary antibody was added (Goat anti-rabbit HRP, 1% milk, TBST 0.2%, 2 h, RT, 1:5000; Sigma). Once washed ...
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bioRxiv - Immunology 2021Quote: ... and then developed at RT by addition of 100 μL of p-nitrophenyl phosphate (pNPP) substrate (Sigma-Aldrich) per well ...
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bioRxiv - Neuroscience 2020Quote: ... followed by blocking for 20 min at RT in 5% normal donkey serum (Sigma, Cat N° D9663-10ML) in 0.1% PBS-Triton X-100 ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were washed three times in 1x PBS before quenching with 0.1 M glycine (Roth) in 1x PBS for 10 min at RT and 0.1 % NaBH4 (Sigma) in 1x PBS for 7 min at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed in PBS at room temperature (RT) then permeabilized with PBS-T (0.1M PBS + 0.2% Triton-X100, Sigma) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... counterstained with DAPI (diluted 1:1000 in PBS) for 3 minutes at RT and mounted with Fluoromont (Sigma).
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bioRxiv - Microbiology 2021Quote: ... followed by staining of cell nuclei for 3 min at RT with 1 μg/ml Hoechst 33342 (Sigma) in PBS−/− ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed again with blocking solution at RT and DNA was counterstained with DAPI (Sigma-Aldrich, D9564). For EdU staining ...
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bioRxiv - Immunology 2022Quote: ... in PBS 30 minutes at RT and blocked with 2% BSA and 5% Donkey Serum (Sigma-Aldrich, D9663) in PBS during 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with secondary antibodies for one hour at room temperature (RT) and mounted using Mowiol (Sigma); Hoechst (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 2 h at RT with anti-αSMA antibody (1:200, Sigma Aldrich, C6198-100UL) and Goat anti Rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... cryo-sections were acetylated using 0,1 M triethanolamine and 0,25% acetic anhydride for 25 min at RT (respectively #90275, #A6404, Sigma). This steps are followed by a pre-hybridization using 2X of SSC ...
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bioRxiv - Cell Biology 2020Quote: ... are stained for 5 minutes at room temperature RT with 0.5μl of PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling (#P7333, Sigma) and then polished with the exosome spin columns (#4484449 ...
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bioRxiv - Bioengineering 2021Quote: ... at RT and nuclear counterstaining was performed using 4’,6-diamidino-2-phenylindole (DAPI, 1.5 μg/mL; Sigma). After brief drying ...
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bioRxiv - Bioengineering 2019Quote: ... Miller.) Reactions were carried out in an RT assay buffer containing: 60 mM Tris (77-86-1, Sigma), 30 mM KCl (7447-40-7 ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 minutes each with PBST at room temperature (RT) with agitation and blocked with 5% Milk Powder (Sigma) in PBST for 1 hr at RT with agitation ...
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bioRxiv - Developmental Biology 2020Quote: ... Fish were stained overnight at room temperature (RT) with a solution of 0.1% Alcian blue 8GX (Sigma Aldrich) w/v ...
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bioRxiv - Immunology 2021Quote: ... in 3% BSA-containing DPBS for 15 min at RT and stained with phalloidin-TRITC (P1951, Sigma Aldrich) and DAPI (D9542 ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was done by extracting total RNA using Tri-reagent (SIGMA) and RT was done with Superscript II (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 30 min at RT with anti-β-actin (1:10’000, A3854 Sigma-Aldrich), which is used as a normalizer.
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bioRxiv - Genomics 2019Quote: ... blots were rinsed three times in PBS and incubated for 10min at RT in 0.02% methylene blue (Sigma) in 0.3M sodium acetate ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation for another 1 hour at RT in a solution containing 2.5% potassium ferrocyanide (Sigma-Aldrich) in 0.1 M PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... and fixed 10 minutes at RT in fixation buffer (10% formaldehyde solution, Sigma Aldrich, in nuclease free water). Cells were washed 2 x and permeabilized O/N in 70% ethanol at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 15 min at RT then permeabilised using 0.5% saponin (Sigma-Aldrich) and stained for 30 min at 4°C for the following intracellular markers ...
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bioRxiv - Neuroscience 2022Quote: ... containing coverslips were fixed for 20 min at room temperature (RT) in 4% (w/v) paraformaldehyde (PFA, Sigma) in 0.15 M phosphate buffer (potassium dihydrogen phosphate (Merck ...
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bioRxiv - Pathology 2023Quote: Liver and renal cortex RNA from EU-exposed mice were extracted using RNAzol RT reagent (Sigma-Aldrich, # R4533). The incorporated EU was detected by click chemistry biotinylation and dot blotting ...
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bioRxiv - Microbiology 2024Quote: ... Some antibodies used were directly coupled to HRP and incubated 1 h at RT (FLAG-HRP, Sigma-Aldrich, A8592 ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 min at RT and then permeabilized for 3 minutes with 0.2% Triton-X 100 (Millipore Sigma). Over-extracted cells were treated with 0.4% Triton-X 100 for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was then subjected to the following washes at RT with agitation: 125 mM SB-10 (Sigma D4266) in 50 mM sodium/10 mM phosphate buffer (VWR BDH9286 ...
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bioRxiv - Cell Biology 2023Quote: ... optionally pre-coated at RT for 20min with 20μl of 0.2mg/ml in DDW concanavalin A (L7647, Sigma) and air-dried.
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were washed and incubated 1 h at RT with peroxidase-conjugated secondary antibodies (anti-mouse, Sigma-Aldrich Cat# A4416 ...
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bioRxiv - Molecular Biology 2023Quote: ... staining, immunoselected transcripts were on-bead digested (10 min, RT) with 2 µL 10 µg/mL RNaseA (Sigma), separated by UREA-PAGE ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 0.5% glutaraldehyde and incubated for 3 min at RT with 5 mg/ml sodium borohydride (Sigma). After washes ...
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bioRxiv - Immunology 2023Quote: ... during 5 minutes at RT followed by blocking with 10 μg/mL human γ-globulin (Sigma-Aldrich, USA) in TBS-0.5% blocking reagent (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA extraction was performed using the RNAzol ® RT reagent following the manufacturer’s recommendations (Sigma-Aldrich, USA). After the DNAse treatment with RQ1 DNAse (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in 66% dichloromethane/33% methanol for 3h at RT and finally cleared in ethyl cinnamate (Sigma 112372). Primary antibodies used were as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... for 30 min at RT and then permeabilized with a solution of 0.5% Triton X-100 (Sigma-Aldrich) and 3 mM EDTA (pH 8 ...