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Citations for Millipore Sigma :
1151 - 1200 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Wako Lab Chemicals) for 4 hours at RT before dilution with ammonium bicarbonate (20 mM; Sigma) and digestion with trypsin (1/50 (w/w) ...
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bioRxiv - Genetics 2019Quote: ... LK03) were pooled in equimolar ratio and fragmented at room temperature (RT) using DNAse I (Sigma). At different incubation time points aliquots were analyzed on an 1.5% agarose gel while the reaction was temporarily stopped by incubation in a dry ice / ethanol bath ...
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bioRxiv - Neuroscience 2020Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Microbiology 2020Quote: ... left 5 min at RT for cell attachment and fixed with 4% paraformaldehyde (PFA) (Sigma P6148) for 10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-qPCR with the FastStart Universal SYBR Green Master (Sigma-Aldrich, Cat No. 4913850001). Primers are listed in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Biophysics 2020Quote: ... and then incubated for 10 min at RT with 25U benzonase nuclease (Millipore Sigma 70746-10KUN)/50 μL sample ...
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bioRxiv - Molecular Biology 2022Quote: RPA and RT-RPA reactions are performed on a variety of commercially available porous membranes (Millipore GF041 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... for 4 minutes at RT and sections were counterstained with haematoxylin (Sigma Chemie GmbH, Steinheim, Germany), dehydrated in ethanol and mounted with Pertex (Histolab ...
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bioRxiv - Cell Biology 2022Quote: Larval preparations were fixed for 20 min at room temperature (RT) with 4% formaldehyde (Sigma #47608) in 1x phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... after fixation of cells with 4% Formaldehyde (10 minutes at RT, Sigma Aldrich, St. Louis, USA). Images were acquired on a Cytation3 imaging reader (BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... and Ms1 and the m7G-capped control were quantified by RT-qPCR with SYBR Green (Sigma). The fraction of treatment-resistant Ms1 was calculated by dividing the amount of transcripts left after the enzymatic treatment (resistant ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS for 1 hour at RT and permeabilized in 1% Triton X-100 (Sigma, #T9284) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 3.7% formaldehyde for 20 minutes at RT and permeabilized in 1% BSA (Sigma-Aldrich) and 0.2% Triton X-100 (Sigma-Aldrich)-containing buffer for 10min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Secondary antibodies were diluted similarly and incubated for 1 h at RT and DAPI (Sigma, D1306) staining for 4 minutes at RT was used for nuclear visualization ...
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bioRxiv - Biophysics 2023Quote: ... and treated for 2 min at RT with pepsin (40 units/mL; Sigma-Aldrich, cat. P6887) diluted in 10 mM HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were stained for 1 h at RT with antibodies against vinculin (hVin1, Sigma; 1:400) and either (phosphoTyr397-FAK (Cat# 44-625G ...
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bioRxiv - Developmental Biology 2023Quote: ... By incubating at RT in darkness for 1 hour in a 1% hydrogen peroxide (Sigma, H1009) solution in 40 % methanol in PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was washed, incubated with DAPI for 5min at RT (1:5000, in PBS (Sigma Aldrich)) and washed ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed at RT for 15 min in 4 % PFA pH 7.4 (Sigma-Aldrich, #P6148-500G). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl RT mix was added (containing 25 mM Trizma hydrochloride solution pH 8 (Sigma Aldrich), 30 mM sodium chloride (Carl Roth) ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated for 1h at RT in blocking buffer containing 10% goat serum (Sigma, G9023) with 0.03 % triton X-100 in PBS and 1:50 Affini Pure Fab Fragment Donkey Anti-Mouse IgG (Jackson Immuno Research Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... in 950 µL PBS for 2 h at room temperature (RT) with Biotin-NHS (H1759, Sigma) in a 1:20 molar ratio (G-CSFR:Biotin-NHS) ...
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bioRxiv - Plant Biology 2024Quote: The gels were incubated 1.5h at RT under gentle shaking with NHS-ester ATTO488 (Sigma, 41698), NHS-ester ATTO647 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... at RT and incubated overnight with a monoclonal Anti-FLAG antibody coupled to horseradish peroxidase (Sigma #A8592 ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (2X) Universal (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Primers used in this study (Table 2) were designed with Primer 3 (http://bioinfo.ut.ee/primer3-0.4.0/) and synthesized by Sigma-Aldrich.
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bioRxiv - Molecular Biology 2020Quote: ... 1 uM final concentration of a mix of SmAgo2 or SmAgo3 forward and reverse primers (Sigma, UK), and 2x Qiagen Fast Cycling PCR master mix (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... Primers were ordered as PAGE or desalted purified oligonucleotides (Table S1) from Sigma-Aldrich (St Louis, MO). Annealed oligos were quantified by BR ds DNA kit using Qubit spectrophotometer (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... Primers used in this work are listed in Appendix Table S2 and were provided by Sigma-Aldrich Co (St ...
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bioRxiv - Systems Biology 2021Quote: ... Primer sequences are shown in Table S1 and were designed in SnapGene and ordered from Sigma Aldrich. Fragments for lentiviral vector construction were generated via PCR using Q5 polymerase (NEB M0491S ...
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bioRxiv - Microbiology 2021Quote: ... Primers (Supplementary Table S5) used in the fecA deletion and complementation were designed using OligoEvaluator (Sigma-Aldrich), ordered from Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... Real-time detection of miRNA expression was performed by using MystiCq miRNA qPCR Assay Primer (Sigma-Aldrich), using Universal Mouse Reference RNA and U6 as reference for mouse and human cells ...
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bioRxiv - Developmental Biology 2019Quote: ... 0.2 μM F and R primers and 0.5 U JumpStart™ Taq DNA Polymerase (Sigma Aldrich, D4184). Specific primers are listed in Table S2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.3 µl EGFP primer/probe mix and 4.95 µl ultrapure nuclease-free water (Sigma-Aldrich, Missouri, USA). Due to a cross-reaction of the G6_10_qPCR primers with E ...
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bioRxiv - Cell Biology 2019Quote: ... All primers used in this study were synthesized and bought from Sigma Aldrich (St. Louis, MO, USA). The following plasmids were obtained from Addgene ...
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bioRxiv - Microbiology 2023Quote: ... the reverse primer CCR5del2 (5’-CATGATGGTGAAGATAAGCCTCACA-3’) was common for both (all Sigma-Aldrich; St. Louis, MO). Two PCR master mixes differing only in the forward primer were prepared at a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... Primers Mpro-expr.-S147Y-for and –rev (table S3) for point mutagenesis were acquired from Sigma Aldrich / Merck (Germany) ...
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bioRxiv - Microbiology 2024Quote: ... acidocaldarius with oligonucleotide primers EP331 and EP332 (Supplementary Table S3) and subsequently cloned into the pET28b (Novagen) plasmid vector using NdeI and BamHI restriction sites using classical restriction-ligation cloning ...
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bioRxiv - Genomics 2024Quote: ... All primers were designed with Primer3 (Untergasser et al., 2012) and purchased from Sigma (Supplementary Table 1).
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bioRxiv - Molecular Biology 2020Quote: ... The PCR reaction mixture contained 2.5 units Taq polymerase and 1 x PCR buffer (D1806, Sigma-Aldrich), 0.25 mM dNTP mix and 0.5 μM of each of the primers ...
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bioRxiv - Neuroscience 2019Quote: ... Mice were genotyped by PCR on tail DNA using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) according to instruction manual ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the GenElute PCR Clean-up kit (Sigma Aldrich, St. Louis, MO, USA) as per instruction manual and triplicates were pooled and diluted 1:5 for a second PCR with Illumina adapter sequences ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged at 1000 × g for 1 minute and set up in new tubes with either anti-Mouse IgG1 control or EZview Red Anti-Flag Affinity Gel (Sigma, St. Louis, MO) (to immunoprecipitate Flag epitope-tagged proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates were then centrifuged at 15,000 rcf for 20 min at 4°C and the supernatants were transferred to another set of Eppendorf tubes containing ∼37.5 µL pre-washed Gammabind Plus Sepharose beads(Millipore Sigma, GE17-0886-01). The beads and lysate were rotated at 4°C for one hour in order to preclear hydrophobic molecules and non-specifics that may interfere with the HLA-IP.
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bioRxiv - Biochemistry 2020Quote: Samples were analysed by mass spectrometry in negative ESI single ion scans set for mass ions of 852.4 m/z (ESI-) (malonyl-CoA, Sigma-Aldrich, Cat. No. M4263), 1032.9 m/z (ESI- ...