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Citations for Millipore Sigma :
1201 - 1250 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were filled to a total 25 µL with PCR grade water (Sigma-aldrich) then placed on a ThermalCycler ...
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bioRxiv - Physiology 2020Quote: PCR reactions were performed using REDTaq® ReadyMix™ PCR Reaction Mix (Sigma-Aldrich, cat. R2523) with the primers listed in Table 1 (500 nM of each ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR-based method was used for detection of Mycoplasma with LookOut Mycoplasma PCR Detection Kit (Sigma) and JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR-based method was used for detection of Mycoplasma with LookOut Mycoplasma PCR Detection Kit (Sigma) and JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified using the GenElute PCR purification kit (Sigma-Aldrich, St. Louis, MO). The indexing PCR was carried out to attach the dual indices (Appendix D6.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Nested PCR was performed by two successive PCR reactions using KOD Xtreme polymerase (EMD Millipore #71975) and following set of primers to amplify the genomic region of INSM1-3’UTR:
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bioRxiv - Developmental Biology 2021Quote: ... All PCR reactions were column purified (High Pure PCR Product Purification kit, cat#11732668001, Sigma Aldrich). 2 ng/μl of purified PCR products were injected into either N2 or SM507 strains along with 100 ng/μl of pRF4 plasmid as a selection marker to create transgenic lines with extrachromosomal arrays.
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bioRxiv - Cell Biology 2020Quote: PCR-based homologous integration cassettes were amplified using the ExpandTM High Fidelity PCR system (Millipore Sigma) from the appropriate plasmid with 80 bp flanking homology to replace the 3’ end of mto2 (primers designed with PPPP at www.bahlerlab.info/resources/)(Bähler et al. ...
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bioRxiv - Microbiology 2022Quote: ... followed by PCR product purification using the GenElute™ PCR Clean-Up Kit (Sigma Aldrich, U.S.A). All sequencing throughout was performed using the Macrogen sequencing service (Macrogen ...
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bioRxiv - Microbiology 2023Quote: The DNA was then subjected to conventional PCR using JumpStart REDTaq ReadyMix PCR Reaction Mix (SIGMA) and genus-specific primers for bacteria detection in human lung tissues ...
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bioRxiv - Immunology 2023Quote: The purified libraries were indexed by PCR using Extract-N-Amp PCR ReadyMix 2x (Sigma, E3004) and sample-specific i5 primers (10x genomics PN2000095 ...
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bioRxiv - Neuroscience 2024Quote: ... Knock-in alleles were then PCR amplified from genomic DNA templates using RedExtract PCR mix (Sigma), and amplicons were visualized on agarose gels with SybrSafe (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the GenElute PCR Clean-Up kit (Sigma-Aldrich #NA1020), digested using DpnI (Promega #R6231) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were plate purified (Millipore Multiscreen filter plates ...
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bioRxiv - Microbiology 2021Quote: ... PCR-based test (Sigma, cat# MP0025) was used to validate the absence of potential mycoplasma contamination in all mammalian cell lines ...
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bioRxiv - Immunology 2020Quote: ... 1X PCR buffer containing MgCl2 (Sigma), 250 µM dNTP (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR product and pET-30b (+) (Novagen) were digested with XbaI and XhoI restriction enzymes ...
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bioRxiv - Neuroscience 2019Quote: ... Dissected retinal tissue was homogenized in RIPA buffer supplemented with Halt protease inhibitor cocktail (Thermo-Fischer, Cat#: 78430) and a phosphatase inhibitor cocktail set II (EMD-Millipore, Cat#: 524625). Protein concentration was estimated with a DC protein assay (BioRad ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed with cold 1× PBS and lysed using the Bio-Plex Pro Cell Signaling Reagent Kit lysis buffer (Bio Rad # 171304006M) supplemented with 2 mM PMSF and 1:200 dilution Phosphatase Inhibitor Cocktail Set III (EMD Millipore # 524627). Lysates were collected and centrifuged at 4°C for 10 min at 15,000×g ...
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bioRxiv - Microbiology 2020Quote: ... Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3) or GFP gene in the presence of polybrene (5 µg/ml; Sigma-Aldrich, Poland). Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... The tissue was enzymatically digested on a MACSMix rotator (set to 16 rpm speed) at 37°C in pre-warmed RPMI/FBS containing Collagenase V (Sigma-Aldrich, C9263), and DNAse I (Roche ...
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bioRxiv - Physiology 2023Quote: ... Mice were anesthetized with 3% isoflurane and an osmotic minipump (Alzet model 2004, #0000298) set to deliver (±)-isoproterenol hydrochloride (Sigma-Aldrich #I5627) dissolved in sterile saline at a dose of 70mg/kg/day for 2 weeks was inserted subcutaneously via a small midline incision in the back ...
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bioRxiv - Pathology 2024Quote: Total RNA was extracted using the Spectrum™ Plant Total RNA Kit with the associated On-Column DNase I Digest Set (Sigma-Aldrich) to remove trace DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Wako Lab Chemicals) for 4 hours at RT before dilution with ammonium bicarbonate (20 mM; Sigma) and digestion with trypsin (1/50 (w/w) ...
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bioRxiv - Genetics 2019Quote: ... LK03) were pooled in equimolar ratio and fragmented at room temperature (RT) using DNAse I (Sigma). At different incubation time points aliquots were analyzed on an 1.5% agarose gel while the reaction was temporarily stopped by incubation in a dry ice / ethanol bath ...
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bioRxiv - Neuroscience 2020Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Microbiology 2020Quote: ... left 5 min at RT for cell attachment and fixed with 4% paraformaldehyde (PFA) (Sigma P6148) for 10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-qPCR with the FastStart Universal SYBR Green Master (Sigma-Aldrich, Cat No. 4913850001). Primers are listed in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated in 1.5% BSA for 1 hr at RT and incubated with 4% PFA (Sigma) for another 24 hr in case of SARS-CoV-2 infected cells ...
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bioRxiv - Biophysics 2020Quote: ... and then incubated for 10 min at RT with 25U benzonase nuclease (Millipore Sigma 70746-10KUN)/50 μL sample ...
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bioRxiv - Molecular Biology 2022Quote: RPA and RT-RPA reactions are performed on a variety of commercially available porous membranes (Millipore GF041 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at RT and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... for 4 minutes at RT and sections were counterstained with haematoxylin (Sigma Chemie GmbH, Steinheim, Germany), dehydrated in ethanol and mounted with Pertex (Histolab ...
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bioRxiv - Cell Biology 2022Quote: Larval preparations were fixed for 20 min at room temperature (RT) with 4% formaldehyde (Sigma #47608) in 1x phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... after fixation of cells with 4% Formaldehyde (10 minutes at RT, Sigma Aldrich, St. Louis, USA). Images were acquired on a Cytation3 imaging reader (BioTek ...
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bioRxiv - Molecular Biology 2021Quote: ... and Ms1 and the m7G-capped control were quantified by RT-qPCR with SYBR Green (Sigma). The fraction of treatment-resistant Ms1 was calculated by dividing the amount of transcripts left after the enzymatic treatment (resistant ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS for 1 hour at RT and permeabilized in 1% Triton X-100 (Sigma, #T9284) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Plant Biology 2024Quote: The gels were incubated 1.5h at RT under gentle shaking with NHS-ester ATTO488 (Sigma, 41698), NHS-ester ATTO647 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and fixed at RT for 15 min in 4 % PFA pH 7.4 (Sigma-Aldrich, #P6148-500G). After washing with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... at RT and incubated overnight with a monoclonal Anti-FLAG antibody coupled to horseradish peroxidase (Sigma #A8592 ...
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bioRxiv - Genetics 2024Quote: ... RT-qPCR was performed using KAPA SYBR® FAST qPCR Master Mix (2X) Universal (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed in 3.7% formaldehyde for 20 minutes at RT and permeabilized in 1% BSA (Sigma-Aldrich) and 0.2% Triton X-100 (Sigma-Aldrich)-containing buffer for 10min on ice ...
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bioRxiv - Biophysics 2023Quote: ... and treated for 2 min at RT with pepsin (40 units/mL; Sigma-Aldrich, cat. P6887) diluted in 10 mM HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were stained for 1 h at RT with antibodies against vinculin (hVin1, Sigma; 1:400) and either (phosphoTyr397-FAK (Cat# 44-625G ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h in the dark at RT and counterstained with DAPI (1:1000, Sigma, T7024). Finally ...
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bioRxiv - Bioengineering 2023Quote: ... Secondary antibodies were diluted similarly and incubated for 1 h at RT and DAPI (Sigma, D1306) staining for 4 minutes at RT was used for nuclear visualization ...
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bioRxiv - Developmental Biology 2023Quote: ... By incubating at RT in darkness for 1 hour in a 1% hydrogen peroxide (Sigma, H1009) solution in 40 % methanol in PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was washed, incubated with DAPI for 5min at RT (1:5000, in PBS (Sigma Aldrich)) and washed ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl RT mix was added (containing 25 mM Trizma hydrochloride solution pH 8 (Sigma Aldrich), 30 mM sodium chloride (Carl Roth) ...