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Citations for Millipore Sigma :
1251 - 1300 of 1544 citations for Non Ab Component of Alzheimer's Disease Amyloid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the wells were washed three times with PBS to remove non-adherent bacteria and then stained with 0.1% crystal violet (Sigma). The plates were incubated for 30 minutes at RT and washed three more times with PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20-50μL concentrated (or equivalent non-concentrated) viral supernatant(s) were added to 5-10×104 suspended cells supplemented with 10μg/mL polybrene (Sigma), then transferred into a 12-well plate for 2-3 days before changing to fresh media ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned medium containing recombinant lentivirus was collected 48hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 μm (Merck Millipore). Samples of these supernatants were applied immediately to target cells together with Polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal antibody against non-muscle myosin heavy chain IIA (1:200 for immunofluorescence; Sigma-Aldrich; catalogue number M8064)
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bioRxiv - Systems Biology 2023Quote: ... Acidified eluates were transferred to MultiScreen®HTS-HV 96-well filtration plates (0.45 μm, clear, non-sterile, Millipore), stacked on 96-well plates and centrifuged for 1 min at 500 ×g to remove in-suspension particles.
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cell Biology 2023Quote: ... LSEC were isolated by gradient density sedimentation of resting non-parenchymal cells on 25–50% Percoll (Merck Millipore, Germany) and purified by using CD146-based isolation on magnetic MicroBeads (MACS ...
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bioRxiv - Immunology 2024Quote: ... Non-specific binding in cells was prevented by incubating with blocking buffer (5% gelatin from bovine skin [Sigma Aldrich]) for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: Non-tumoral brain tissue samples from euthanized mice were collected and lysed in RIPA lysis buffer (#20-188, Millipore) containing protease inhibitors (Complete Mini ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei from tagged cells were separated from non-tagged nuclei by incubation with anti-GFP (Sigma; cat#SAB4301138-100UL) and anti-myc (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was blocked with 10% normal donkey serum (Cat. No. SLBW2097, Sigma-Aldrich, Inc, St. Louis, MO) in 0.1 M PBS containing 0.3% Triton X-100 (Cat ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting cell suspension was underlayed with 200 ul of 1% non-acetylated BSA in HBSS (Sigma: 55021C-1000ML) using a gel loading pipette tip ...
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Biophysics 2019Quote: ... The resulting digest was then reduced (addition of 4 μL × 500 mM dithiothretol, Sigma, in AB; 10 min. shaking at 60 °C) and alkylated (addition of 12 μL × 500 mM iodoacetamide ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...
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bioRxiv - Immunology 2021Quote: Plasma antibody levels of K11 and C9-tagged 5L7 were measured by ELISA using plates coated overnight at 2 μg/mL with either purified SIVmac239 gp140 FT or mouse anti- rhodopsin (C9) monoclonal Ab (EMD Millipore, 6B9575), respectively ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times and HRP-conjugated Abs (goat anti-mice IgG and IgM) (Sigma-Aldrich, St. Louis, MO, USA) were added at 1:1500 dilution in 0.5% HSA/PBST (100 µL/well ...
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bioRxiv - Cancer Biology 2022Quote: ... Wild-type AB zebrafish larvae were dechorionated at 2 days post-fertilization (dpf) by incubating in 0.1 mg/mL pronase (Sigma-Aldrich, MO, USA) solution for 10 min at 28°C ...
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bioRxiv - Cell Biology 2022Quote: ... shaking at 60 °C) and alkylated (addition of 12 µL x 500 mM iodoacetamide, Sigma, in AB; 30 min. shaking at RT). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... the fractions containing ABs were combined and the buffer was changed to PBS buffer using Amicon Ultra-4 30 kDa (Millipore EMD, USA). AB concentration was determined using Bradford dye and glycerol was added up to 50% for storage at -20C.
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 1.5 x103/cm2 on 13 mm coverslips in 24 well-plates and cultured in DMEM containing 2% with human AB serum (HS; Sigma Aldrich, H4522) in a 5% humidified CO2 atmosphere at 37°C ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Cancer Biology 2024Quote: Human hepatocellular carcinoma cell lines (HLF, SNU-499, Hep3B) were cultured in Dulbeccós modified Eaglés medium without glucose (DMEM; Sigma-Aldrich AB, Stockholm, Sweden) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... and pellet was washed once with PBS and another time with 1 ml ice-cold 155 mM ammoinium bicarbonate (AB, Sigma-Aldrich, FL40867) until finally resuspended in same buffer to 5000 cells/µl ...
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bioRxiv - Developmental Biology 2020Quote: ... three Bap1-gRNA targeting the 180 bp region upstream of the Bap1 TSS and one non-targeting-gRNA (Supplementary Table 5) (Joung et al. 2019) were selected and synthesized (Sigma). Each oligo was annealed and cloned into the sgRNA (MS2)-puro plasmid (Addgene 73795 ...
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bioRxiv - Microbiology 2020Quote: This drug susceptibility assay was performed essentially as described [63] and is based on only live cells reducing the blue and non-fluorescent viability indicator dye resazurin sodium salt (‘Alamar blue’; Sigma) to pink ...
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bioRxiv - Neuroscience 2022Quote: ... groups of 15-20 female flies were placed in vials containing standard cornmeal/sugar/agar food alone for non-treatment control experiments or standard food mixed with 1mg/ml of water-soluble test compounds crocin (cat# 17304 Sigma), rifaximin (cat# Y0001074 ...
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bioRxiv - Neuroscience 2020Quote: ... Mission ShRNA bacterial Glycerol stock NM_026582) and scramble control (Mission TRC2 PlkO.5-PURO Non-Mammalian shRNA control Plasmid) were purchased from Sigma-Aldrich. Wnt3a plasmid pLCN-Wnt3a-HA (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in non-denaturing lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 90 μL of cell culture was loaded onto a Cellstar non-transparent white 96-well flat-bottom plate (Sigma-Aldrich). OD660 of cells was taken for each sample ...
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bioRxiv - Immunology 2019Quote: ... SmKT and SmKB were formulated in the Adjuvant Adaptation (ADAD) vaccination system with non-hemolytic saponins from Quillaja saponaria (Qs; Sigma) and the synthetic aliphatic diamine AA0029 emulsified in a non-mineral oil (Montanide ISA763A ...
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bioRxiv - Genetics 2020Quote: ... the dish was transferred to another Nikon inverted microscope that was equipped with a CellEctor single-cell isolation system (Molecular Machines and Industries) and cell adhesion was loosened by exchanging the culture medium with a PBS-based non-enzymatic dissociation reagent (Sigma). Within ∼30 min of applying dissociation reagent ...
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bioRxiv - Molecular Biology 2019Quote: ... These base media were then supplemented with 84 mg/mL 13C615N4 L-arginine plus 146 mg/mL 13C615N2 L-lysine or the corresponding non-labeled amino acids (all from Sigma) to generate either heavy or light media ...
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bioRxiv - Microbiology 2019Quote: ... A sequencing ladder was generated by reverse transcription of 1 pmol non-treated in vitro-transcribed ppp-RNAIII leader supplemented with 0.5 mM deoxynucleotide triphosphate mix each (Sigma-Aldrich), and 1 mM of the corresponding dideoxynucleotide triphosphate (Jena Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotted PDVF membranes were blocked in 5% (w/v) non-fat milk powder or 4% (w/v) Bovine Serum Albumin (BSA, Sigma) in PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... Monolayer cell cultures and MCSs were incubated in serum-free KSFM medium containing non-fluorescent calcein-AM (1 mM in DMSO, Sigma) at a final concentration of 1 μM ...
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bioRxiv - Microbiology 2019Quote: ... patient sera were pre-incubated for 10mins with anti-histone single-chain variable fragment (ahscFv; 200 μg/ml, synthesis described previously (18)) or with non-anticoagulant N-acetyl heparin (200 μg/ml; Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... Activated p38MAPKα was added to a final concentration of 60 pM for reactions with 1nM non-phosphorylated GST-MK2 (Millipore Corporation ...
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bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
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bioRxiv - Immunology 2021Quote: ... and SP-R210(KO) RAW 264.7 cells were detached using non-enzymatic cell dissociation medium (Sigma-Aldrich Cat#C1544-100ML) and washed in PBS containing 2% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420 ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable cell lines were established using gene specific mission shRNAs (Cat# TRCN0000000595, TRCN0000000592, TRCN0000230910) or non-targeting control (Sigma-Aldrich). Cells were plated at 5×105 in a 6-well dish in growth medium containing 8μg/mL polybrene ...
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bioRxiv - Biochemistry 2021Quote: The fractions of the non-denaturing detergent lysates that were subjected to limited proteolysis were incubated with different concentrations Proteinase K (Sigma), as described before [1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Microbiology 2020Quote: ... and the RNA from the lysed non-persister cells was completely degraded using RNAse A (Sigma-Aldrich, St. Louis, USA) before proceeding with the total RNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... The plating solution consisted of 5% gold non-cyanide solution (Sifco 5355 Gold Plating Solution, Neuralynx) with 1% Poly(ethylene glycol) (PEG) addictive (BioUltra, 8,000, Sigma-Aldrich) mixed in a 75% v/v PEG to gold ratio ...
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bioRxiv - Microbiology 2021Quote: ... Staining was carried out with cells grown in the presence of non-growth inhibitory concentrations (30 µg/ml; Sigma Aldrich) of the outer membrane-permeabilising agent Polymyxin B nonapeptide ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... Tester cells attachment to a culture dish was weaken by replacement of the culture medium with a non-enzymatic cell dissociation solution (Millipore) for 15-30 min in a cell culture incubator (37°C ...