Labshake search
Citations for Millipore Sigma :
1101 - 1150 of 1544 citations for Non Ab Component of Alzheimer's Disease Amyloid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... The non-targeting control siRNAs (MISSION siRNA Universal Negative Control #1, SIC001) were purchased from Sigma Aldrich.
-
bioRxiv - Cell Biology 2023Quote: ... FACS proteins were collected from the non-or Dox-treated cells and diluted in RIPA buffer (Millipore). Biotinylated proteins were isolated by streptavidin pull-down (SA-Pd ...
-
bioRxiv - Cancer Biology 2023Quote: ... and shCtrl-2 (MISSION® pLKO.1-puro non-mammalian shRNA Control Plasmid DNA, SHC002, Sigma-Aldrich).
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked using 5% non-fat dry milk or 5% bovine serum albumin (BSA) (Sigma-Aldrich) in Tris-buffered saline (TBS ...
-
bioRxiv - Bioengineering 2024Quote: ... The non-defined one with gel strength of 50–120 (called type II) from Sigma-Aldrich (G6650) was used.
-
bioRxiv - Cancer Biology 2024Quote: ... or control cells expressing non-mammalian targeting control shRNA (pLKO.1-Puro_shCtrl; MISSION® SHC002, Sigma-Aldrich) were generated using HEK293T cells ...
-
bioRxiv - Neuroscience 2024Quote: ... 1X non-essential amino acids (cat#: 11-140-050; Gibco, 400 mM Monothioglycerol (cat#: M6145; Millipore Sigma), and 5 mg/mL human insulin (cat# ...
-
bioRxiv - Physiology 2024Quote: Lentivirus expressing non-target shRNA (SHC002) and shRNAs targeting Msi2 were generated according to manufacturer’s instruction (Sigma). HEK293FT cells were co transfected with vectors expressing shRNA and lentivirus packaging plasmids ...
-
bioRxiv - Genetics 2024Quote: ... Bacillus non-essential gene knock out library mutants were grown in kanamycin (7.5 µg/mL (Sigma, K1377)) ...
-
bioRxiv - Cell Biology 2020Quote: ... wildtype AB zebrafish embryos microinjected with pig tubulin-atto565 were mechanically dechorionated and mounted in 1% low melting-point agarose (Sigma) in E3 medium supplemented with 20% w/v Iodixanol (Sigma ...
-
bioRxiv - Immunology 2019Quote: ... then were incubated with both mouse anti-IgG4 and a rabbit polyclonal anti-human IL-5Rα Ab (Sigma PA5-25159) or rabbit IgG in the first step ...
-
bioRxiv - Immunology 2019Quote: ... saturated in blocking solution (PBS-BSA 5%) for 1 hour and finally incubated with primary Abs: rabbit anti-mouse laminin (Sigma), Alexa fluor 594 Goat anti-mouse IgM (Thermofisher ...
-
bioRxiv - Immunology 2019Quote: ... For the IHC staining cells were stained with a primary antibody (see below for Ab details) and visualized with diaminobenzidine (DAB, Sigma) solution ...
-
bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
-
bioRxiv - Bioengineering 2019Quote: The ab-and adsorption of small molecules by the membranes was quantified by immerging them in 10µM rhodamine B (Sigma-Aldrich) in PBS for 2h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein eluted fractions (as determined by Abs at 280 nm) were pooled and concentrated by ultrafiltration (Amicon Ultra-15 30KD, Millipore). All synthetic peptides were synthesized ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 5% heat-inactivated AB human serum, antibiotics (penicillin, 200 U/ml; and streptomycin, 0.1 mg/mL) and L-glutamine (1 mM) (Sigma-Aldrich) at a concentration of 1 x 107 cells/mL ...
-
bioRxiv - Developmental Biology 2022Quote: 10 AB strain wild type embryos per condition per experiment were fixed in either 4% paraformaldehyde (PFA, Sigma Aldrich F8775) in 1X phosphate buffered saline (PBS ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
-
bioRxiv - Immunology 2020Quote: Primary human NK cells were incubated after enrichment for 16 hours at 37°C in 3 ng/mL IL-15 (Miltenyi) in RPMI media supplemented with 10% Human AB serum (Sigma). Cells were washed with PBS ...
-
bioRxiv - Immunology 2020Quote: ... the membranes were incubated with the appropriate horseradish-peroxidase conjugated secondary Ab (1:5000 in TBST + 2% BSA; 2 h at room temperature; anti-mouse A4416 and anti-rabbit A0545, Sigma). The immunoreactivity was detected by ECL reagents (Amersham) ...
-
bioRxiv - Pathology 2021Quote: ... Cytokine profiling analysis was performed by Eve Technologies (Calgary, AB Canada) using the Milliplex MAP Human Cytokine/Chemokine Panel (Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
-
bioRxiv - Immunology 2020Quote: ... Freshly-isolated neutrophils were suspended at 106 or 5×106 cells/mL in RPMI media (containing 10% human AB serum (Sigma)) and incubated at 37°C and 5% CO2 for up to 18h ...
-
bioRxiv - Immunology 2020Quote: ... mononuclear cells were thawed and plated at 106 cells per cm2 in 20 ml RAB5 (RPMI with 5% human serum AB (Sigma), 2 mM L-alanyl-L-glutamine ...
-
bioRxiv - Immunology 2021Quote: ... 400 μl containing 500 PFU/ml of SARS-CoV-2 virus were then added to the Ab solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... mouse monoclonal anti Rho-GDI (Santa-Cruz Biotechnology, 1:500) and mouse monoclonal anti-FLAG M2 Ab (Sigma, 1:1000), anti-ERK1/2 (Cell Signaling Technology ...
-
bioRxiv - Immunology 2021Quote: Splenocytes were stimulated with plate-bound anti-CD3 Ab for 20 hours followed by a 4-hour treatment with 20 ng/ml of phorbol myristate acetate (PMA) (Sigma) and 1 μM of ionomycin (Calbiochem ...
-
bioRxiv - Cancer Biology 2022Quote: ... and subcultured for 72 h in NK MACS medium supplemented with 5% human serum male AB plasma (#H4522, Sigma-Aldrich) and human IL-2 IS (200 IU/ml ...
-
bioRxiv - Plant Biology 2022Quote: ... 100 mM ammonium bicarbonate [AB], 10 mM Tris[2-carboxyethyl]phosphine hydrochloride, 50 mM 2-chloroacetamide, 0.2% protease inhibitor [Sigma, P8340]), and incubated in the dark at room temperature for 30 min ...
-
bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
-
bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
-
bioRxiv - Immunology 2023Quote: ... were isolated from PBMCs using anti-CD14-antibody-coated magnetic beads (Miltenyi) and cultured in RPMI1640 supplemented with 10% human AB serum (Sigma), pen/strep ...
-
bioRxiv - Cancer Biology 2024Quote: ... antibodies were purchased from Santa Cruz Biotechnology. Anti-p21 (Ab-1, catalog no. OP64) and anti-Noxa (catalog no. OP180) antibodies were purchased from EMD Millipore. Anti-cleaved PARP (catalog no ...
-
bioRxiv - Biophysics 2021Quote: ... non-specific binding was blocked with 0.2% gelatin from cold water fish skin (#G7765 Sigma-Aldrich Chimie, Lyon) in TS for 30min ...
-
bioRxiv - Cell Biology 2019Quote: Cells were transiently transfected with 100 nM of siRNA constructs or a non-related siRNA (siScramble) (Sigma-Aldrich) using Lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Biophysics 2022Quote: ... in PBS for 10 minutes and blocked for non-specific antibody binding with 10% horse serum (Sigma-Aldrich) in PBS for 40 minutes ...
-
bioRxiv - Biophysics 2022Quote: ... and subsequently blocked with 0.5 % non-fat dried milk (AppliChem) in TBST using the Snap i.d.® 2.0 Protein Detection System (Millipore). The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W ...
-
bioRxiv - Genomics 2020Quote: ... LECs were incubated in the starvation medium as non-proliferative control or treated with 100ng/mL nocodazole (Sigma) as mitosis control ...
-
bioRxiv - Developmental Biology 2021Quote: ... Non-cellular debris was removed by gradient centrifugation for 10 minutes at 2000g using Histopaque 1119 (Sigma-Aldrich). Interface cells were collected and washed ...
-
bioRxiv - Cell Biology 2021Quote: ... TACC3 (5’ GAGCGGACCUGUAAAACUA 3’) and a non-targeting oligo against Luciferase was used as control (Sigma, Custom order). RNAi Max (Invitrogen ...
-
bioRxiv - Bioengineering 2021Quote: ... Sodium Tripolyphosphate (TPP) and MEM non-essential amino acid solution were supplied by Sigma-Aldrich (Merck, Darmstadt, Germany). Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Biophysics 2020Quote: ... Non-ionic surfactant TritonTM X-100 and fluorescent dye Calcein were obtained from Sigma-Aldrich (St.Louis, MO USA). Chelating agent EDTA ...
-
bioRxiv - Cell Biology 2020Quote: Statistical significance was assessed using t-test or non-parametric Mann-Whitney rank sum test (Sigma plot 12.0) if datasets were not normally distributed (according to Anderson-Darling normality test) ...
-
bioRxiv - Cell Biology 2022Quote: ... pLKO lentiviral vectors expressing non-targeting shRNA and shRNAs targeting SOX2 and SOX15 were purchased from Sigma-Aldrich.
-
bioRxiv - Neuroscience 2021Quote: ... and subsequently stained with primary antibody overnight in 5% non-fat Milk in PBS + 0.05% Tween 20 (Sigma). The following primary antibodies were used ...
-
bioRxiv - Neuroscience 2022Quote: ... mice were treated with the non-selective NOS inhibitor N(G)-Nitro-L-arginine methyl ester (LNAME, Sigma). LNAME (10mg/ml made up with sterile saline ...
-
bioRxiv - Bioengineering 2022Quote: ... Bis-cysteine crosslinking peptide VPM (GCNSVPMSMRGGSNCG) or non-degradable crosslinking agent DTT (1,4-dithiothreitol; Sigma, 3483-12-3) was dissolved in diH2O at 27.3 mM ...
-
bioRxiv - Bioengineering 2020Quote: ... Non-specific binding sites were blocked by incubation in 10% (v/v) normal donkey serum (D9663; Sigma-Aldrich) and 1% (v/v ...
-
bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA or 5% non-fat milk in TBST and incubated with primary antibodies (translin,1:100,000; FMRP, 1:10,000, Millipore) overnight at 4°C ...