Labshake search
Citations for Millipore Sigma :
1301 - 1350 of 1463 citations for Non Ab Component of Alzheimer's Disease Amyloid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Non-specific binding was determined under the same conditions in the presence of 20 μM unlabeled ryanodine (Millipore-Sigma, #559276).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Non-specific binding was determined under the same conditions in the presence of 20 μM unlabeled ryanodine (Millipore-Sigma, #559276).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Post incubation cells were washed twice more with PBS and detached with 5mL non-enzymatic cell dissociation solution (Sigma, UK). Cells were pelleted by centrifugation for 10 min at 1000xg ...
-
bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
-
bioRxiv - Molecular Biology 2024Quote: ... 2 mL of clarified lysate per sample (crosslinked and non-crosslinked) was incubated with 80 µL of anti-FLAG M2 magnetic beads (Sigma) prewashed 5 times in NT-P buffer ...
-
bioRxiv - Cancer Biology 2024Quote: ... TTK (sc-36758-V) or a non-targeting control (sc-108080) in triplicate using Polybrene Transduction Reagent (5 µg/ml; Millipore). Single-cell colonies were subsequently isolated by selection with puromycin (5 µg/ml ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein extracts of puromycin pulsed and non-puromycin pulsed control cells were subject to immunoblot (as above) using anti-puromycin antibody (clone 12D10, 1:1000, Sigma).
-
bioRxiv - Cell Biology 2024Quote: ... Lipolysis was assessed from the release of FFA and glycerol in the secretion samples using non-esterified fatty acids measurement kit (FUJIFILM Wako Chemicals Europe GmbH) and free glycerol reagents (G7793 and F6428, Sigma) respectively and normalised to total protein contents.
-
bioRxiv - Genomics 2020Quote: ... Primary antibodies were detected using anti-rabbit secondary antibodies (1:5000 dilution, Anti-Rabbit IgG HRP Linked Whole Ab Sigma Cat# GENA934-1ML). The band intensities were quantified with Image J (Schneider et al ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... The following primary antibodies were applied for 24 h (or 48 h for anti-cFos antibodies) at 4°C: mouse anti-APC/CC1 Ab-7 (Millipore, OP80, 1:100), chicken anti-mCherry (Abcam ...
-
bioRxiv - Physiology 2020Quote: ... The other half of the conditioned media was subjected to IgG pull-down step (2 µl for 9 ml CM) (Anti-mouse IgG monoclonal Ab; Sigma-Aldrich; Cat#M9144) which was used as a negative control for this assay ...
-
bioRxiv - Microbiology 2020Quote: ... and rested overnight at 37°C/5%CO2 in RPMI-1640 medium supplemented with 10% heat-inactivated human AB serum (Sigma Aldrich, Missouri, USA), 2 mM L-glutamine and 1 mM sodium pyruvate before infection ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The CAR T cell product was cultivated in RPMI+10%FBS+1%PS at 37°C with 5% CO2 for experiments and cryopreserved as 10×106 cells/mL in 1 mL 90% heat-inactivated Human AB Serum (Sigma-Aldrich, Cat.#H4522) +10% DMSO in liquid nitrogen ...
-
bioRxiv - Systems Biology 2024Quote: ... Abs were then reacted with 10x molar excess of dibenzocyclooctyne-S-S-N-hydroxysuccinimidyl (DBCO-S-S-NH) ester (Sigma Aldrich, 761532-1mg) for 2 hours at room temperature in the dark ...
-
bioRxiv - Cell Biology 2024Quote: ... Control conditions included a set of micropattterned grids that were not incubated in antibody (No Ab) and another set of micropatterned grids that were treated with monoclonal antibodies to VZV glyco-protein E (gE; EMD Millipore Cat# MAB8612).
-
bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
-
bioRxiv - Physiology 2022Quote: ... streptomycin (50 µM from a stock concentration of 15 mM in distilled water, to non-specifically block mechano-sensitive channels, Sigma-Aldrich), HC-030031 (10 µM from a stock concentration of 50 mM in DMSO ...
-
bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked for a minimum of 1 hour at room temperature in 5% (w/v) non-fat milk or 5% (w/v) BSA (A4503-50G; Sigma Aldrich) in TBS-T (1x TBS and 0.1% Tween-20 ...
-
bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
-
bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... cell lysates were subjected to immunoprecipitation overnight using an anti-mouse HSF2 (Santa-Cruz) and a non relevant IgG (Sigma-aldrich) as a negative control that were pre-incubated 1h at RT with Protein G UltraLink Resin beads (53132 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Bound dARC1 CA was eluted in non-reducing buffer (50 mM Tris-HCl, 150 mM NaCl, 300 mM Imidazole) and Carboxypeptidase A (CPA, Sigma C9268) was added at a ratio of ∼ 100 mg dARC1 per mg CPA ...
-
bioRxiv - Cancer Biology 2019Quote: ... After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA) in phosphate-buffered saline (PBS) with 0.5% Tween 20 (Sigma-Aldrich, Belgium), the membranes were incubated overnight at 4°C with the primary antibodies (Table 1) ...
-
bioRxiv - Neuroscience 2021Quote: ... we demonstrated that these manipulations did not interfere with non-social investigatory/play behavior and habituation (towards amyl acetate (Sigma-Aldrich)/ (-)-carvone (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
-
bioRxiv - Neuroscience 2020Quote: ... To inhibit the proliferation of non-neuronal cells a mitotic inhibitor 5-fluoro-2’-deoxyuridine (FDU, final concentration 10 μM, Sigma-Aldrich) was added with the change of the medium.
-
bioRxiv - Cell Biology 2021Quote: ... the cells were treated for 30 minutes with 1 mM of L-glutamine plus 1x concentration of either minimum essential amino acids mix (EAAs)(Thermo-Scientific #11130036) or EAAs and non-essential amino acis mix (NEAAs) (1:1 mix of EAAs with NEAAs, Sigma #M7145) either in the presence or absence of 2.5 mM of glucose (Thermo scientific #A2494001 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was blocked with 5% non-fat milk in TBST buffer and was incubated subsequently with monoclonal ANTI-FLAG® M2 antibody (Sigma) and goat anti-mouse IgG (H+L)-HRP conjugate (Bio-rad) ...
-
bioRxiv - Molecular Biology 2022Quote: shRNA pLKO.1 plasmids expressing non-targeting control shRNA (NTC: SHC002) or anti- DDX39B shRNAs (Sh3: TRCN0000286976; and Sh5: TRCN0000294383) were purchased from Millipore Sigma. The construct pLCE-DDX39B was generated by cloning the coding sequence of DDX39B into the pLCE vector ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membranes were blocked with 5% non-fat milk and incubated with the following antibodies: anti-FLAG (1:1000, Cat. #F1804; Sigma-Aldrich), and anti-HA (1:1000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids containing shRNAs targeting STING (shSTING-1 #TRCN0000161345, shSTING-2 #TRCN0000163029) or non-targeted (scrambled) shRNA (shNT #SHC016-1EA) were purchased from Sigma Aldrich. STING-IRES-GFP and DN-IRES-GFP lentiviral plasmids were generated by inserting the cDNA of STING or STING-DN ...
-
bioRxiv - Cancer Biology 2019Quote: ... Samples were analyzed with either a 3h 15 min non-linear gradient or a 1h 23 min linear gradient from 2.4% acetonitrile (ACN, Sigma, 34998-4L), 0.1% FA to 32% ACN ...
-
bioRxiv - Microbiology 2019Quote: ... then washed once in PBS to remove non-adherent bacteria and incubated for 1 hour in DMEM GlutaMAX™ supplemented with 400 µg/mL gentamicin (Sigma), 100 µg/mL polymyxin B (Sigma ...
-
bioRxiv - Cell Biology 2020Quote: ... His-Rap1b was converted to active form by loading with a 10-fold excess of GMPPCP (non-hydrolyzable analogue of GTP, #M3509-25MG, Sigma-Aldrich) for 24 h at +4 °C in Exchange buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells and WT or mutant A1R expressing HEK 293 cells were harvested using a non-enzymatic cell dissociation solution (Sigma-Aldrich) and washed with PBS prior to counting ...
-
bioRxiv - Cell Biology 2019Quote: For immunoprecipitation two samples with 1×108 cells each were adjusted to a concentration of 5×105 cells/ml in fresh medium and were left non-induced or were induced with a final concentration of 100 ng/ml doxycycline (Sigma-Aldrich) for 15 h ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The titre is described as the dilution at which absorbance was greater than of the negative control (IgG from non-immunised horses; Sigma, UK) plus two standard deviations.
-
bioRxiv - Immunology 2019Quote: ... Heat-mediated antigen retrieval was performed with 10mM sodium citrate and non-specific staining reduced by incubation in 5% (v/v) goat serum (#G9023, Sigma Aldrich) for 30 mins at RT ...
-
bioRxiv - Microbiology 2019Quote: Growth versus non-growth phenotypes in bulk liquid culture were assessed using a cell proliferation assay with the non-toxic green fluorescent membrane linker dye PKH67 (Sigma-Aldrich). To ensure that Methylobacterium cells could be easily distinguished from background events in flow cytometry ...
-
bioRxiv - Biochemistry 2021Quote: ... 400 μL lysis buffer with gentle non-ionic detergent (50 mM Tris buffer, pH 8, 150 mM NaCl, 1% IGEPAL CA-630 (Cat# I8896, Sigma Aldrich), 10% glycerol and 1x protease inhibitor (Cat# A32965 ...
-
bioRxiv - Immunology 2021Quote: ... pLKO.1 empty vector was from Open Biosystems, and pLKO.1 control shRNA (scramble shSCR, SHC002 and non target shNTgt, SHC016) from Sigma-Aldrich. Lentiviral vectors carrying these constructs were produced by calcium phosphate transfection of 293FT cells with shRNA constructs in combination with packaging vectors psPAX2 ...
-
bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK) 293T cells and human non-small-cell lung cancer (NSCLC) A549 cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2020Quote: ... Single cells were separated from non-dissociated tissue debris by sieving them through Falcon™ Cell Strainers (pore diameter of 70μm, Sigma-Aldrich) and seeded onto 24- or 96-well plates coated with poly-L-lysine-(PLL ...
-
bioRxiv - Cancer Biology 2019Quote: ... The pLenti pLKO-non-target shRNA control vector (SHC002) and two different pLenti-mouse shRNA vectors for FYN were selected from Sigma Aldrich MISSION® shRNA Vectors ...
-
bioRxiv - Molecular Biology 2021Quote: The HsaBR sequence and its corresponding non-binding control HsaBR(R340E) (709 bp) in the bacterial expression vector pGEX-3X (Millipore Sigma) was cloned by Paul Sullam’s Laboratory and described elsewhere ...
-
bioRxiv - Immunology 2022Quote: Serum samples were diluted 1:2 in serum matrix for analysis using the Milliplex Non-Human Primate Magnetic Bead Panel as per the manufacturer’s instructions (Millipore, Burlington, MA). Concentrations for G-CSF ...
-
bioRxiv - Developmental Biology 2022Quote: 300k SV-HUC-1 cells were seeded and transfected with 3µg MISSON pLKO.1-puro non-mammalian targeting control shRNA (Sigma, Cat# SHC002) or shExoc5 (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated overnight at 4°C with primary antibodies diluted either in 5% non-fat dry milk in TBST or 5% Bovine Serum Albumin (BSA, Sigma, # A7906) + 0.02% sodium azide in TBST ...
-
bioRxiv - Cancer Biology 2022Quote: ... purified the protein using Ni- resin and exchanged the bound nucleotide for non-hydrolysable GTP analogue GMPPCP using alkaline phosphatase beads (Sigma-Aldrich) and following the protocol of John et.al ...