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Citations for Millipore Sigma :
1151 - 1200 of 1463 citations for Non Ab Component of Alzheimer's Disease Amyloid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Heat shock and non-heat shock HCT116 cells were washed with PBS before fixing with 1% formaldehyde (Sigma, 252549) in PBS for 15 minutes and processing for ChIP-seq.
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bioRxiv - Biochemistry 2020Quote: ... The reaction progress was determined by SDS–PAGE under non-reducing conditions after treating the samples with 0.1 mM S-methylmethanethiosulfonate (Sigma) to quench the reaction ...
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bioRxiv - Biochemistry 2021Quote: ... EMSA reactions were then run on a non-denaturing 0.5X TBE 6% polyacrylamide gel and stained with ethidium bromide (Sigma). Band intensities were quantified using ImageJ.
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated by adding a non lysing formulation of 0.5 mM D-Luciferin substrate (23) (Sigma-Aldrich) and measuring luciferase activity for 1 second on a plate Reader Infinite 200 PRO (Tecan®) ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were blocked for 1 hour with 5% non-fat dry milk in PBS/0.1% tween-20 (Sigma Aldrich), and subsequently incubated overnight at 4 °C with respective primary antibodies ...
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bioRxiv - Biophysics 2022Quote: ... Non-specific protein adsorption to the coverslip was prevented by adding 0.005% (v/v) Tween-20 (P2287, Sigma-Aldrich) to the solution ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin, Bovine Serum, 12659, Merck Millipore) powder solved in 1 × TBST ...
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bioRxiv - Neuroscience 2020Quote: ... The stamps were removed from nanopillar substrates and non-coated area was blocked with Pluronic F-127 (P2443, Sigma) solution ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding was blocked using 5% of normal goat serum (16210064, Life technology) with 2.5% BSA (10735078001, Sigma) for 60 min ...
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bioRxiv - Cancer Biology 2019Quote: ... a sample mixture of 10 μg protein and non-reducing sample buffer was loaded on a polyacrylamide gel containing gelatin (0.25 mg/ml; Sigma). The gel was then soaked (36 h ...
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bioRxiv - Cancer Biology 2022Quote: ... for 30 minutes at room temperature and non-specific blocking with 1% bovine serum albumin (BSA; A2153, Sigma-Aldrich) and 10% normal goat serum (NGS ...
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bioRxiv - Zoology 2019Quote: ... two containing 160 nM cortisol (10% 1,2,6,7-3H-cortisol, Perkin Elmer, Waltham, MA, and 90% non-labeled cortisol, C-106, Sigma-Aldrich) to measure total binding ...
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Impact of DNA sequences in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complexbioRxiv - Biochemistry 2020Quote: ... The completion of the reaction was determined by SDS–PAGE under non-reducing conditions after treating the sample with 0.1 mM S-methylmethanethiosulfonate (Sigma) to quench the reaction ...
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bioRxiv - Immunology 2020Quote: ... after which non-adherent cells were washed off using phosphate buffered saline (PBS, with MgCl2 and CaCl2, Sigma-Aldrich) and the adherent macrophages were cultured for 2 more days ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204, Merck Millipore) and probed with the respective primary and secondary antibodies ...
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bioRxiv - Immunology 2019Quote: ... resuspended in RPMI-10% FBS containing 40ng/mL CTLA4-Ig (CD152/Fc chimera, non-cytolytic, from mouse, Sigma C4358), plated in 24-well culture plates at a density of 2 x 106 cells/well and cultured for an additional 24 hours (BMDCCTLA4-Ig) ...
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bioRxiv - Immunology 2019Quote: ... TBK1 (SHCLND-NM_013254) or non-Target shRNA Control Plasmid DNA were all obtained from Sigma (MISSION shRNA Plasmid DNA). In brief ...
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... or a non-targeting control shRNA (TRCN0000231489) with no targets in the human genome were obtained from Sigma-Aldrich. The SLFN11 expression vector was obtained from VectorBuilder along with an empty vector control with the same lentiviral backbone ...
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bioRxiv - Microbiology 2020Quote: ... Non-adherent cells were removed and the remaining monocytes were maintained in DMEM with 5% human serum (HS; Millipore) and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... and non-selective P2-purinoreceptor antagonist pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid (PPADs) ([25mg/kg] in PBS; Sigma).27,44–46 Following recording of a pre-inhibitor response ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... with 100 μM of the non-selective exchange factor directly activated by cAMP (EPAC1/2) inhibitor ESI-09 (Sigma)80 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Odyssey blocking buffer TBS (Licor) or 5% non-fat milk or 1% BSA (Sigma, A7906) for 1 hour before over-night incubation at 4°C with primary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: The Toxoid sequence pltB_pltA(E133A)_cdtB(H160Q)-6xHis and its corresponding non-binding control pltB(S35A)_pltA_cdtB-6xHis in the bacterial expression vector pET28b (Novagen) was kindly provided by Jorge Galan and described elsewhere ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral knockdown vectors targeting mouse prkcb and non-targeting control (SCR) were purchased from Sigma-Aldrich (St. Louis, MO) in the pLKO.1 backbone ...
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bioRxiv - Physiology 2022Quote: ... The same procedure was followed for Glycolysis Stress Test in non-buffered DMEM without glucose and glutamine (Sigma-Aldrich). Cells were monitored in basal conditions and in response to D-glucose (10 mM ...
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bioRxiv - Microbiology 2022Quote: ... Media used for both Vir Ind and non-Vir Ind were sterilized using 1L 0.22 µm vacuum filtration units (Sigma) after pH adjustment.
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bioRxiv - Neuroscience 2022Quote: ... After a further two washes in PBS and a single wash in non-saline Tris-buffered solution (T6066, Sigma), samples were encased with a glass coverslip and Fluorsave mounting agent (Millipore ...
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bioRxiv - Immunology 2022Quote: ... Fractions containing IgG were identified by SDS-PAGE under reducing/non-reducing conditions and concentrated using Centrifugal Filters (Millipore). Protein concentration was quantified with the BCA Assay Kit (Beyotime ...
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bioRxiv - Developmental Biology 2022Quote: ... Free (non-esterified) fatty acids were measured using Roche’s Free Fatty Acid Kit (half-micro test) (Sigma Aldrich – 11383175001), which is based on the enzymatic conversion of free fatty acids to acyl CoA by acyl-Co A synthetase.
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bioRxiv - Cancer Biology 2023Quote: Two individual pre-designed human PLXNB2 sgRNA CRISPR-Sanger clones and one non-targeting control vector (Sigma-Aldrich #0020) were ordered from Sigma as glycerol stock (Sigma-Aldrich #HS5000013567 ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes with the transferred proteins were blocked with 10% (w/v) non-fat milk in TBS with 0.05% (v/v) Tween 20 (TBST, Sigma) for 40 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat dry milk diluted in Tris-buffered saline with 0.1% Tween 20 (cat no. P9416, Sigma) (TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous SA1 and SA2 were immunoprecipitated with 10 µg SA1 and SA2 antibodies or non-specific IgG control (Sigma) conjugated to protein G dynabeads (Dynal ...
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bioRxiv - Immunology 2023Quote: ... islets were incubated (37°C for 6 min) in a non-enzymatic cell dissociation solution (Sigma-Aldrich; #C5914-100ML) then vortexed to disrupt the islets.
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bioRxiv - Microbiology 2023Quote: ... the wells were washed three times with PBS to remove non-adherent bacteria and then stained with 0.1% crystal violet (Sigma). The plates were incubated for 30 minutes at RT and washed three more times with PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 20-50μL concentrated (or equivalent non-concentrated) viral supernatant(s) were added to 5-10×104 suspended cells supplemented with 10μg/mL polybrene (Sigma), then transferred into a 12-well plate for 2-3 days before changing to fresh media ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned medium containing recombinant lentivirus was collected 48hrs after transfection and filtered through non-pyrogenic filters with a pore size of 0.45 μm (Merck Millipore). Samples of these supernatants were applied immediately to target cells together with Polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal antibody against non-muscle myosin heavy chain IIA (1:200 for immunofluorescence; Sigma-Aldrich; catalogue number M8064)
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bioRxiv - Systems Biology 2023Quote: ... Acidified eluates were transferred to MultiScreen®HTS-HV 96-well filtration plates (0.45 μm, clear, non-sterile, Millipore), stacked on 96-well plates and centrifuged for 1 min at 500 ×g to remove in-suspension particles.
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bioRxiv - Immunology 2023Quote: Lentiviral vectors containing validated shRNA sequences targeting expression of human PRKCD (accession NM_006254) or a non-target control were obtained from Sigma (catalog numbers TRCN0000010193 ...
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bioRxiv - Cell Biology 2023Quote: ... LSEC were isolated by gradient density sedimentation of resting non-parenchymal cells on 25–50% Percoll (Merck Millipore, Germany) and purified by using CD146-based isolation on magnetic MicroBeads (MACS ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting cell suspension was underlayed with 200 ul of 1% non-acetylated BSA in HBSS (Sigma: 55021C-1000ML) using a gel loading pipette tip ...
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bioRxiv - Molecular Biology 2023Quote: Articular cartilage explants from the non-OA donors were treated with 3 μM of Wnt agonist CHIR99021 (Sigma-Aldrich) or with 1 μL/mL of its vehicle (V ...
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bioRxiv - Molecular Biology 2023Quote: Lentiviral shRNA vectors targeting PSIP1 (PSIP1 ShRNA1 TRCN0000298567, PSIP1 shRNA2 TRCN0000286345) and non-targeting control were procured from Sigma Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... Cy3-STxB was generated by conjugating Cy3 dye onto STxB according to the manufacture manual of CY®3 Ab Kit (PA330000, Sigma-Aldrich).
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bioRxiv - Biophysics 2019Quote: ... The resulting digest was then reduced (addition of 4 μL × 500 mM dithiothretol, Sigma, in AB; 10 min. shaking at 60 °C) and alkylated (addition of 12 μL × 500 mM iodoacetamide ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...