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Citations for Millipore Sigma :
1201 - 1250 of 1463 citations for Non Ab Component of Alzheimer's Disease Amyloid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Plasma antibody levels of K11 and C9-tagged 5L7 were measured by ELISA using plates coated overnight at 2 μg/mL with either purified SIVmac239 gp140 FT or mouse anti- rhodopsin (C9) monoclonal Ab (EMD Millipore, 6B9575), respectively ...
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bioRxiv - Microbiology 2019Quote: ... The a-P28 antibody was conjugated with the Cy3 fluorescent dye using the Cy®3 Ab Kit GE Healthcare (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times and HRP-conjugated Abs (goat anti-mice IgG and IgM) (Sigma-Aldrich, St. Louis, MO, USA) were added at 1:1500 dilution in 0.5% HSA/PBST (100 µL/well ...
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bioRxiv - Cancer Biology 2022Quote: ... Wild-type AB zebrafish larvae were dechorionated at 2 days post-fertilization (dpf) by incubating in 0.1 mg/mL pronase (Sigma-Aldrich, MO, USA) solution for 10 min at 28°C ...
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bioRxiv - Cell Biology 2022Quote: ... shaking at 60 °C) and alkylated (addition of 12 µL x 500 mM iodoacetamide, Sigma, in AB; 30 min. shaking at RT). Next ...
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bioRxiv - Cell Biology 2023Quote: ... and pellet was washed once with PBS and another time with 1 ml ice-cold 155 mM ammoinium bicarbonate (AB, Sigma-Aldrich, FL40867) until finally resuspended in same buffer to 5000 cells/µl ...
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bioRxiv - Molecular Biology 2024Quote: ... the fractions containing ABs were combined and the buffer was changed to PBS buffer using Amicon Ultra-4 30 kDa (Millipore EMD, USA). AB concentration was determined using Bradford dye and glycerol was added up to 50% for storage at -20C.
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 1.5 x103/cm2 on 13 mm coverslips in 24 well-plates and cultured in DMEM containing 2% with human AB serum (HS; Sigma Aldrich, H4522) in a 5% humidified CO2 atmosphere at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: Human hepatocellular carcinoma cell lines (HLF, SNU-499, Hep3B) were cultured in Dulbeccós modified Eaglés medium without glucose (DMEM; Sigma-Aldrich AB, Stockholm, Sweden) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Developmental Biology 2020Quote: ... three Bap1-gRNA targeting the 180 bp region upstream of the Bap1 TSS and one non-targeting-gRNA (Supplementary Table 5) (Joung et al. 2019) were selected and synthesized (Sigma). Each oligo was annealed and cloned into the sgRNA (MS2)-puro plasmid (Addgene 73795 ...
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bioRxiv - Microbiology 2020Quote: This drug susceptibility assay was performed essentially as described [63] and is based on only live cells reducing the blue and non-fluorescent viability indicator dye resazurin sodium salt (‘Alamar blue’; Sigma) to pink ...
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bioRxiv - Neuroscience 2022Quote: ... groups of 15-20 female flies were placed in vials containing standard cornmeal/sugar/agar food alone for non-treatment control experiments or standard food mixed with 1mg/ml of water-soluble test compounds crocin (cat# 17304 Sigma), rifaximin (cat# Y0001074 ...
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bioRxiv - Neuroscience 2020Quote: ... Mission ShRNA bacterial Glycerol stock NM_026582) and scramble control (Mission TRC2 PlkO.5-PURO Non-Mammalian shRNA control Plasmid) were purchased from Sigma-Aldrich. Wnt3a plasmid pLCN-Wnt3a-HA (Addgene ...
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bioRxiv - Molecular Biology 2021Quote: HCT116 p53+/+ and p53-/- cells were harvested in non-denaturing lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 90 μL of cell culture was loaded onto a Cellstar non-transparent white 96-well flat-bottom plate (Sigma-Aldrich). OD660 of cells was taken for each sample ...
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bioRxiv - Immunology 2019Quote: ... SmKT and SmKB were formulated in the Adjuvant Adaptation (ADAD) vaccination system with non-hemolytic saponins from Quillaja saponaria (Qs; Sigma) and the synthetic aliphatic diamine AA0029 emulsified in a non-mineral oil (Montanide ISA763A ...
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bioRxiv - Genetics 2020Quote: ... the dish was transferred to another Nikon inverted microscope that was equipped with a CellEctor single-cell isolation system (Molecular Machines and Industries) and cell adhesion was loosened by exchanging the culture medium with a PBS-based non-enzymatic dissociation reagent (Sigma). Within ∼30 min of applying dissociation reagent ...
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bioRxiv - Molecular Biology 2019Quote: ... These base media were then supplemented with 84 mg/mL 13C615N4 L-arginine plus 146 mg/mL 13C615N2 L-lysine or the corresponding non-labeled amino acids (all from Sigma) to generate either heavy or light media ...
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bioRxiv - Microbiology 2019Quote: ... A sequencing ladder was generated by reverse transcription of 1 pmol non-treated in vitro-transcribed ppp-RNAIII leader supplemented with 0.5 mM deoxynucleotide triphosphate mix each (Sigma-Aldrich), and 1 mM of the corresponding dideoxynucleotide triphosphate (Jena Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... Western blotted PDVF membranes were blocked in 5% (w/v) non-fat milk powder or 4% (w/v) Bovine Serum Albumin (BSA, Sigma) in PBS-T ...
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bioRxiv - Cancer Biology 2019Quote: ... Monolayer cell cultures and MCSs were incubated in serum-free KSFM medium containing non-fluorescent calcein-AM (1 mM in DMSO, Sigma) at a final concentration of 1 μM ...
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bioRxiv - Microbiology 2019Quote: ... patient sera were pre-incubated for 10mins with anti-histone single-chain variable fragment (ahscFv; 200 μg/ml, synthesis described previously (18)) or with non-anticoagulant N-acetyl heparin (200 μg/ml; Sigma).
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bioRxiv - Cancer Biology 2019Quote: ... Activated p38MAPKα was added to a final concentration of 60 pM for reactions with 1nM non-phosphorylated GST-MK2 (Millipore Corporation ...
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bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
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bioRxiv - Immunology 2021Quote: ... and SP-R210(KO) RAW 264.7 cells were detached using non-enzymatic cell dissociation medium (Sigma-Aldrich Cat#C1544-100ML) and washed in PBS containing 2% fetal bovine serum (FBS) ...
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420 ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable cell lines were established using gene specific mission shRNAs (Cat# TRCN0000000595, TRCN0000000592, TRCN0000230910) or non-targeting control (Sigma-Aldrich). Cells were plated at 5×105 in a 6-well dish in growth medium containing 8μg/mL polybrene ...
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bioRxiv - Biochemistry 2021Quote: The fractions of the non-denaturing detergent lysates that were subjected to limited proteolysis were incubated with different concentrations Proteinase K (Sigma), as described before [1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Microbiology 2020Quote: ... and the RNA from the lysed non-persister cells was completely degraded using RNAse A (Sigma-Aldrich, St. Louis, USA) before proceeding with the total RNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... The plating solution consisted of 5% gold non-cyanide solution (Sifco 5355 Gold Plating Solution, Neuralynx) with 1% Poly(ethylene glycol) (PEG) addictive (BioUltra, 8,000, Sigma-Aldrich) mixed in a 75% v/v PEG to gold ratio ...
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bioRxiv - Microbiology 2021Quote: ... Staining was carried out with cells grown in the presence of non-growth inhibitory concentrations (30 µg/ml; Sigma Aldrich) of the outer membrane-permeabilising agent Polymyxin B nonapeptide ...
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Endometrial Gap Junction Expression - Early Indicators of Endometriosis and Integral to InvasivenessbioRxiv - Cell Biology 2021Quote: ... Tester cells attachment to a culture dish was weaken by replacement of the culture medium with a non-enzymatic cell dissociation solution (Millipore) for 15-30 min in a cell culture incubator (37°C ...
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bioRxiv - Biophysics 2020Quote: ... Calcein loaded liposomes were separated from non-encapsulated (free) calcein by gel filtration using a Sephadex G-75 column (Sigma). We used an elution buffer of 10 mM MOPS ...
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bioRxiv - Cell Biology 2021Quote: ... the nitrocellulose membranes were blocked with 5% non-fat dry milk in phosphate-buffered saline (PBS) 1X/0.1% Tween 20 (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Non-polarized and polarized T84 cells were pre-treated with or without the ezrin inhibitor NSC668394 (20 μM, EMD Millipore), the myosin light chain kinase (MLCK ...
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bioRxiv - Cancer Biology 2022Quote: Leukemia cells were seeded at a density of 2.5 x 105 cells/well of a non TC-treated 12-well plate in complete medium containing polybrene (10µg/mL, EMD Millipore), and then transduced with lentivirus by centrifugation at 2,500rpm for 90 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking of non-specific antigenic sites was achieved over 1 hour using a 0.2% Triton X-100/0.1M PBS (Sigma-Aldrich®) (PBST ...
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bioRxiv - Neuroscience 2022Quote: ... Dbh−/− mice were treated with either saline or the non-selective DA receptor antagonist flupenthixol (0.25 mg/kg) (Sigma-Aldrich) to determine if excessive DA signaling contributes to grooming in these mice.
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bioRxiv - Microbiology 2020Quote: ... Caco-2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Media Preparation Unit, Peter Doherty Institute) supplemented with 1X non-essential amino acids (Sigma-Aldrich), 20 mM HEPES ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was then blocked with 5% non-fat milk in phosphate buffer saline (PBS)-0.1% Tween20 (Sigma-Aldrich, P1379) for 1h at RT and probed with specific primary antibodies (α-p21 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat. M7145) and sodium pyruvate (Gibco cat ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in blocking buffer (5% non-fat milk or 5% FBS diluted in TBS-T buffer (0.05% Tween-20 (Sigma-Aldrich) in tris-buffered saline (TBS) ...
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bioRxiv - Cell Biology 2019Quote: ... and the non-Rab Ras superfamily small GTPases (HRas and Arf1) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described previously [27 ...
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bioRxiv - Immunology 2019Quote: ... The labeled B cells were then attached to coverslips that had been coated with 0.25 µg/cm2 of the non-stimulatory M5/114 anti-MHC II monoclonal antibody (Millipore, #MABF33). SPT was carried out at 37°C using an Olympus total internal reflection fluorescence microscopy (TIRFM ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The PTX-treated and control (non-PTX treated) cells were plated at a density of 100,000 cells per well in poly D-lysine (Sigma-Aldrich) coated ...
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bioRxiv - Genomics 2020Quote: ... embryoid bodies were transferred to ultra-low-attachment 6-well TC plates in neural induction media (DMEM/F12, 1% Glutamax, 1% non-essential amino acids and 10 µg/ml heparin (Sigma). After another 4-5 days ...
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bioRxiv - Cancer Biology 2020Quote: ... containing cGAS shRNA sequences (shcGAS #1, TRCN0000428336; shcGAS #2, TRCN0000149811) and non-target shRNA control vector (shScramble, SHC016) were purchased from Sigma.