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Citations for Millipore Sigma :
1051 - 1100 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The flow rate was set to 400 µl/min using a binary buffer system consisting of buffer A (10 mM ammonium formate (Sigma), 0.15% [v/v] formic acid (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... in accordance with the manufacturer’s protocol and treated with genomic DNAse using DNASE 70-On Column DNase I Digestion set (Sigma-Aldrich) to eliminate DNA contamination ...
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bioRxiv - Immunology 2024Quote: ... Iron free media was supplemented with set concentrations of human holotransferrin (R&D systems, 2914-HT-001G/Sigma Aldrich, T0665) ranging from 0.0002-0.625 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The flow rate of 400 µl/min was set using a buffer system consisted of buffer A (10 mM ammonium formate (Sigma), 0.15% formic acid (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... whole cell extracts were obtained by resuspending cell pellets in cell extract buffer (20 mM Tris [pH 7.0], 1× Protease Inhibitor Cocktail Set I (Sigma-Aldrich)) and transferred to sterile screw cap tubes containing silica beads ...
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bioRxiv - Cancer Biology 2021Quote: ... FastStart PCR Kit (Sigma-Aldrich) was used for PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... KAPA SYBR PCR kit (Sigma) was used for QPCR (Light Cycler 480 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×PCR buffer (Sigma P2192), 400 nM of primers (Table S.6 ...
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bioRxiv - Microbiology 2023Quote: ... 1× PCR Buffer (Sigma-Aldrich), 3 mM MgCl2 ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were purified using GenElute™ PCR Clean-Up Kit (Sigma-Aldrich, USA) followed by cloning of the same using pGEM-T Easy Vector System I ...
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bioRxiv - Developmental Biology 2022Quote: ... Several probes were produced by PCR amplification (KAPA2G Fast ReadyMix PCR kit, Sigma Israel, KK5101). cDNA templates were synthesized by RNA precipitation followed by reverse transcription PCR ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... PCR products were purified with the High Pure PCR Product Purification Kit (Sigma Aldrich, 11732676001) and cloned into the pcDNA3.1 Hygro+ mammalian expression vector (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v Alizarin Red S (Sigma-Aldrich, St. Louis, USA, 10 min incubation, RT) in distilled water was used to stain the mineralized extracellular matrix (ECM) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked for 30 min at RT in 1% BSA (Millipore Sigma, #A-4503) in PBS ...
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bioRxiv - Immunology 2019Quote: ... Eluted A20 was stabilized by incubation with iodoacetamide (Sigma; 30 mM, 30 min at RT). The reaction was terminated by addition of an equivalent amount of β-mercaptoethanol ...
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bioRxiv - Pathology 2019Quote: ... Cells were washed 3x 5min each at RT in PBS-T (0.1% Tween20 (Sigma P9416). Secondary was performed at RT in the dark for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... at RT under agitation followed by quenching by addition of 0.125 M Glycine (Sigma G8898) for 5 min under agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed for 1h at RT using 20% normal goat serum (S26, Sigma-Aldrich) in Tris-NaCl blocking buffer (TNB) ...
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bioRxiv - Neuroscience 2020Quote: ... then washed overnight at RT with PBS containing 1% (v/v) Triton-X 100 (Sigma) and then with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong Solution (Millipore #2504), blocked for 30 min in 5% BLOTTO ...
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bioRxiv - Cell Biology 2021Quote: Coverslips were coated at RT with 5ug/mL Poly-L-lysine solution (Sigma-Aldrich, P4707) for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: RT-qPCR was carried out using FastStart Universal SYBR Green Master Mix (Rox) (Sigma Aldrich) in an Applied Biosystems QuantStudio 6 ...
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bioRxiv - Microbiology 2020Quote: ... Mannose inhibition: biotinylated HHL was incubated with methyl mannose (200 mM; 30 min, RT; Sigma) prior to HHL staining.
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bioRxiv - Immunology 2021Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... washed with 3x PBS at RT and stained with alizarin red S (Sigma Aldrich, A5533) 1% in ddH2O ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Immunology 2019Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong solution (Millipore #2504), blocked for 30 min in 5% blotto ...
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Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibersbioRxiv - Cell Biology 2020Quote: ... followed by incubation for 30 min at RT with anti-laminin (1:100; Sigma L9393). Secondary AlexaFluor antibodies (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... in the medium for 10 min at RT and quenched with 2.5 M Glycine (Sigma) for 2 min at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... and cross-linked for 10 min at RT by the addition of formaldehyde (F1635, SIGMA) to a final concentration of 1.2% ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed at RT with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MO, USA) in PBS ...
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bioRxiv - Immunology 2021Quote: ... blocked for 1 hr at room temperature (RT) in 5% Skim Milk Powder (Sigma-Aldrich) in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... sections were subjected to 0.5 % hydrogen peroxide solution at RT for 20 mins (H1009, Sigma) in methanol to quench endogenous peroxidases ...
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bioRxiv - Developmental Biology 2022Quote: ... were fixed for 15 min at RT in 4% (w/v) paraformaldehyde (Sigma, Cat. #158127) and permeabilized with 0.1% Triton-X-100 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells and bone marrow tissue cells were treated with RNAzol RT (Sigma-Aldrich), and total RNA was isolated according to the manufacturer’s instruction and saved at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... Sections were incubated in primary antibody for 1 hour at RT: rabbit Anti-GLUT1 (Millipore), chicken Anti-GFP (Aves) ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were further washed at RT for 5min with 1mL 100µM Ammonium bicarbonate (Sigma-Aldrich), 10mM DTT and then with 1mL 100µM Ammonium bicarbonate ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with SYBR Green Quantitative RT-qPCR Kit (Sigma-Aldrich; Cat. No. QR0100) using GAPDH as a housekeeping gene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated overnight at room temperature (RT) with anti-VRK3 (1/1000 #HPA056489, Sigma) and anti-Phalloidine (1/200 #8940S ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections were stabilized at RT for 5 min and blocked with 10% donkey serum (Sigma) + 0.3% Triton X-100 (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... followed by 10 min fixation at RT by adding 20 μL of 4% paraformaldehyde (Sigma) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated for 2 h at RT in casein blocking buffer (Sigma Aldrich), then overnight at +4°C with rabbit anti-CtBP2 antibody (Abcam #ab128871 ...
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bioRxiv - Cell Biology 2023Quote: Macrophages were fixed with 4% formaldehyde for 20 min at RT in the dark (Sigma). After fixation ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 1h at RT with 1% BSA + 0.3% TritonX-100 (Sigma Aldrich T9284) in DPBS ...
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bioRxiv - Plant Biology 2020Quote: RNA extraction was performed using 100 mg of frozen ground tissue (leaves) and treated with RNase-Free DNase by the On-Column DNase I Digestion Set (Sigma-Aldrich). For the subsequent analysis only RNA samples with integrity greater than 8.0 were used ...
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bioRxiv - Biophysics 2022Quote: ... A bulk calibration data was initially set up by measuring the fluorescence intensity of 41-μm-thick channels passivated with 1% Pluronic F127 (Sigma-Aldrich) and filled with antibody solutions at concentrations of 3.75 ...
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bioRxiv - Neuroscience 2021Quote: ... parental crosses to obtain experimental animals were set up on standard medium supplemented with 400 μM all-trans-retinal (ATR, Sigma #R2500) food or on a standard medium mixed with 100% ethanol for no ATR controls ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...