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Citations for Millipore Sigma :
1101 - 1150 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Red fluorescent signals was obtained using detection reagent red (Sigma-Aldrich: DUO92008) and chromogen signals was acquired using detection reagent brightfield (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... and chromogen signals was acquired using detection reagent brightfield (Sigma-Aldrich: DUO92012).
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bioRxiv - Cell Biology 2022Quote: ... Chromogenic detection was performed followed by counterstaining with hematoxylin (GHS332, Sigma Aldrich). Staining was visualized using a Nikon Eclipse Ni-U bright-field microscope (Nikon ...
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bioRxiv - Molecular Biology 2021Quote: ... Detection was carried out using Immobilon Crescendo Western HRP substrate (WBLUR00500, Millipore).
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and visualized by enhanced chemiluminescence detection (LuminataForte Western HRP substrate, Merck Millipore). Digital images were acquired on ChemiDoc XRS System (Bio-Rad ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse IgG or anti-human IgG detection antibodies (Sigma) were added at 1:10000 dilution with a final room temperature incubation for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... AP activation was determined through C3c detection using mAb WM-1 (Sigma). All assays were performed in triplicate and normalized to positive (100% ...
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bioRxiv - Biochemistry 2022Quote: ... followed by chemiluminescence detection using Immobilon Forte Western HRP substrate (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained using the AldeRed ALDH detection assay (Sigma/Merck, SRC150) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, #WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... Protein detection were performed with Immobilon Western Chemiluminescent HRP Substrate (ECL, Millipore). Band intensities were analyzed by ImageJ software.
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... and detection was performed via Immobilon Western Chemiluminescent HRP Substrate (WBKLS0100, Millipore) on an Odyssey Fc Dual-Mode Imaging System (LICOR Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... A peroxidase-coupled secondary antibody and the enhanced chemiluminescence detection system (Millipore) were used to detect the immune complexes using Image Quabt LAS 4000 biomolecular imager (GE Healthcare).
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bioRxiv - Neuroscience 2023Quote: ... for detection using mouse anti-ASCL1 and anti-rabbit OLIG2 (Millipore/AB9610) on nitrocellulose followed by the appropriate Alexa680/IR800 secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... the ammonia detection assay was completed as per manufacturer instructions (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
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bioRxiv - Cancer Biology 2021Quote: ... and incubated for 10 min at RT in DPBS/DNases (Sigma, DN25) solution ...
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bioRxiv - Pathology 2022Quote: ... rinsed in PBS and fixed (4% paraformaldehyde, Sigma, in PBS, 15min., RT), permeabilized (0.1% Triton X-100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were fixed with formaldehyde (Sigma-Aldrich®, 1%, 10 min, RT) and quenched with glycine (0.125 M ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quenching 5 min at RT with 125 mM glycine (Sigma). Cultures were spun down ...
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bioRxiv - Developmental Biology 2021Quote: ... and stored at RT in PBS containing 0.2% gelatin (Sigma Aldrich, #G1393), 0.5% Triton X-100 (Sigma Aldrich ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and coated for 1hr RT with 25 μg/ml laminin (Sigma, L2020) diluted in a buffer containing 150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... permeabilized (1% Triton-X, Sigma-Aldrich, in PBS; 10 min. at RT) and blocked (2% bovine serum albumin ...
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bioRxiv - Immunology 2022Quote: ... fixed for 10 min at RT with 4% paraformaldehyde (PFA: Sigma-Aldrich) in 0.1 M Phosphate Buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... DNA oligonucleotides (RT-qPCR primers) were obtained from Sigma-Aldrich (Rehovot, Israel). Mouse monoclonal antibodies against ARC (sc-17839 ...
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bioRxiv - Microbiology 2024Quote: ... with commercial RT-qPCR primers (KiCqStart SYBR Green Primers, Millipore Sigma KSPQ12012) and was assayed in Applied Biosystems QuantStudio 3 Real Time PCR System with standard cycling conditions ...
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bioRxiv - Genomics 2023Quote: ... RT-qPCR was performed using FastStart Universal SYBR Green Master (Sigma-Aldrich) and primers are given in Supplementary Table 9.
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bioRxiv - Physiology 2024Quote: ... for 3h at RT and blocked with PBS/5% casein (Sigma-Aldrich) over night at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... at RT for 1 h and detected by ECL reagent (WBKLS0500, Millipore), then exposed to film.
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bioRxiv - Microbiology 2024Quote: ... Total RNA was isolated using RNAzol® RT (Sigma-Aldrich, Steinheim, Germany) with homogenization lasting 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... Lastly cells were washed three times with 0.2% Triton X-100 solution for about 5 minutes each time and mounted in Fluoromount (Sigma, F4680) with 0.1% DAPI in it ...
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed an additional three time with TBS-T and one time with TBS before 3 minute exposure with ECL HRP substrate (Millipore) and imaged on a BioRad ChemiDoc system ...
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bioRxiv - Cell Biology 2023Quote: ... at which time cultures were washed three times with pre-warmed HBSS containing 100 ug/mL heparin (Sigma-Aldrich H3393) to remove any transiently adhered ...
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bioRxiv - Immunology 2024Quote: ... infected cells at different time-points were washed 3 times in 1xPBS and lysed with 0.1% Triton X-100 (#X100 Sigma-Aldrich) in H2O ...
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bioRxiv - Genetics 2024Quote: ... ∼20mg pulverised frozen liver tissue was dounced ten times with pestle A and ten times with pestle B (Sigma # 8938) in 1ml hypotonic lysis buffer solution B containing 10 mg/ml BSA (Sigma # 126609) ...
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bioRxiv - Pathology 2021Quote: All samples were stained at the same time under the same conditions using a modified Fontana-Masson Kit staining protocol (Sigma-Aldrich Procedure #HT200). Prior to staining ...
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bioRxiv - Microbiology 2024Quote: Cell-supernatant was harvested at the specific time-points post-infection and the lactate content of the sample was estimated using the Lactate Assay Kit (Sigma, Catalog Number-MAK064) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... At indicated time (hpi) 500 uM 4SU (Sigma) was added to cell culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... three times with LiCl wash buffers (Millipore Sigma), and two times with TE buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS three times and trypsinized (Sigma). Cell pellets were additionally washed three times with PBS before fixation ...
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bioRxiv - Cell Biology 2020Quote: ... washed three times with PBS (Sigma Aldrich, USA) and blocked for 45 minutes with 0,1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times in pre-warmed PBS (Sigma), twice in pre-warmed DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... washed three times with PBS (1x; Sigma; P5493) and blocked for 1 h with blocking buffer (BB ...
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bioRxiv - Neuroscience 2023Quote: ... washed four times in ethanol (Sigma-Aldrich, 459836) and coated onto PVC tubing (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: A time-course assay employing trypsin (Sigma Aldrich) was devised to investigate the degradation of the adenylate kinases ...
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bioRxiv - Microbiology 2019Quote: ... Size of the PCR products was verified by gel electrophoresis and purified using an Amicon Ultra 0.5 ml kit (Millipore).
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bioRxiv - Immunology 2020Quote: ... PCRs for genotyping of bacterial colonies after transformation were performed using the KAPA2G Fast ReadyMix kit (Sigma Aldrich, #KK5102) with custom designed primers and the following cycling conditions ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Microbiology 2022Quote: The PCR products were inserted into the pET-30 Ek/LIC vector according to manufacturer’s instructions (LIC Kit, Novagen) (Table S2) ...