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Citations for Millipore Sigma :
1201 - 1250 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... for 1 h at RT in microscopy buffer (5% w/V BSA (Sigma), 0,06% w/V saponin (Sigma ...
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bioRxiv - Plant Biology 2019Quote: Reverse transcription (RT) was performed on total RNA extracted using Tri-Reagent (Sigma) from untreated and UV-C treated plants (time points 0 ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilised for 20min at RT using PBS/0.25% Triton X-100 (Sigma). Cells were blocked for 30min at RT with blocking solution (PBS-0.25% Triton X-100 plus BSA 1%) ...
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bioRxiv - Neuroscience 2022Quote: ... blocked for 1 hour at room temperature (RT) with 6% donkey serum (Sigma) + 0.3% Triton X-100 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 % glycerol for GII RTs) with an Amicon Ultra-15 (30k) concentrator (Millipore) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... PBS pH 7.2) at RT for 1h and incubated with Acta2 antibody (Sigma) at 40C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... blocked for 1 hour at room temperature (RT) with 6% donkey serum (Sigma) + 0.3% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 24h later at least 1h at RT and stained with crystal violet (Sigma). PFU were counted from each dilution in order to determine the infectious titer (in PFU/mL ...
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bioRxiv - Bioengineering 2024Quote: ... and fixation initiated at RT in 10% neutral buffered formalin (NBF, Sigma-Aldrich), followed by fixation/storage at 4°C ...
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bioRxiv - Pathology 2023Quote: ... washed and fixed 8 h (RT) in 4% paraformaldehyde (P6148, Sigma Aldrich, USA). Tissues were embedded in Tissue-Tek OCT Compound (Sakura Finetek ...
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bioRxiv - Pathology 2023Quote: ... for 60 min at RT and visualized using 3,3-diaminobenzidine (DAB) (Sigma, 11718096001) for 10 min at RT ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The tissues were stained overnight at RT using DAPI (1 µg/µl) (Sigma) diluted at 1× PBS.
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bioRxiv - Biochemistry 2024Quote: ... for 30 min at RT shaking and alkylated with 20 mM Iodoacetamide (Sigma) for 30 min at RT shaking ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... After fixation (4% paraformaldehyde in PBS pH 7.4, Sigma Aldrich; 20 min, RT) and permeabilization of the cells (0.1% Triton X-100 in PBS pH 7.4 plus 1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... at RT for 2 hours and the nuclei were labelled using DAP (Sigma). Cells were stained with alexa-fluor tagged Lectin (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was homogenized in a glass Dounce tissue grinder (25 times with pestle A, 25 times with pestle B; Sigma, Cat#D8938) in 2mL ice-cold EZ Nuclei Lysis Buffer (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma] ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed three times with RPMI media (Sigma) prior to addition of melan A-specific T cells (healthy donor-derived ...
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bioRxiv - Microbiology 2022Quote: ... washed two times with PBS pH 7.2 (Sigma-Aldrich) and resuspended in 2 ml of PBS ...
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bioRxiv - Cell Biology 2022Quote: ... At indicated times auxin (3-indolo acetic acid, Sigma) was added at a final concentration of 1-2.5 mM (table S4) ...
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bioRxiv - Microbiology 2021Quote: ... Fixed cells were washed three times with DPBS (Sigma) + 0.02% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Two times concentrated drug solution including tamoxifen (Sigma-Aldrich), paclitaxel (Selleckchem ...
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bioRxiv - Evolutionary Biology 2020Quote: ... then rinsing three times with milli-Q water (Millipore) and lastly soaking in 70% ethanol for 2 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 5 mL ...
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bioRxiv - Microbiology 2022Quote: ... 10 times using Amicon centrifugal filter units (Merck Millipore) with a 100 kDa cut-off to a final volume of 20 mL ...
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bioRxiv - Microbiology 2023Quote: ... three times using 100 kDa amicon filters (Merck, Millipore) and centrifuging at 3’000 × g for 6 min ...
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bioRxiv - Cell Biology 2023Quote: ... this time using 100 mM NaN3 (Sigma-Aldrich, S8032) to allow the lineup of animals ...
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bioRxiv - Genomics 2024Quote: ... then rinsing three times with milli-Q water (Millipore) and lastly soaking in 70% ethanol for 2 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... E11.5 embryos were dissected into ice-cold PBS and associated yolk sacs used for rapid genotyping using the Extract-N-Amp Tissue PCR kit as recommended by the manufacturer (Sigma). During genotyping ...
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bioRxiv - Developmental Biology 2020Quote: ... genomic DNA was extracted from single blastocysts by placing each blastocyst in a microtube containing 4.4 µl Extraction buffer (REDExtract-N-Amp Tissue PCR Kit, Millipore Sigma) mixed with 1.1 µl of Tissue Prep Buffer ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Microbiology 2021Quote: ... PCR-positive colonies were grown and the vector was extracted using GenElute Plasmid Miniprep Kit following manufacturer’s protocol (Sigma-Aldrich). The vector was then transformed into expression strain E ...
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bioRxiv - Microbiology 2020Quote: ... the PCR reaction described above was repeated using genomic DNA isolated using GenElute™ Bacterial Genomic DNA Kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... All DNA fragments were cleaned-up with GenElute™ PCR Clean-Up Kit (Sigma Aldrich Co., St. Louis, MO, USA) prior to PCR analysis ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR fragments were analyzed using Agarose Gel Electrophoresis and purified from Gel using the GenElute Gel Extraction Kit (Sigma, NA1111) or MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... profiling at Broad between January and December 2018 and were routinely tested for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma)
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were cloned into pET28a using infusion PCR (Novagen, Darmstadt, Germany). The recombinant vector was transformed into E ...
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bioRxiv - Cell Biology 2020Quote: For cell adhesion studies monoclonal antibodies against vinculin-FA detection (Sigma-Aldrich, 1:400), pMLC-intracellular tension (Cell Signaling ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Detection of S was done with mouse monoclonal ANTI-FLAG M2 antibody (Sigma F3165) and Alexa Fluor 680 AffiniPure Goat Anti-Mouse IgG ...
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bioRxiv - Neuroscience 2021Quote: Detection of brain infarction was performed by a triphenyl tetrazolium chloride (TTC, Sigma-Aldrich) staining at 24 h after HI surgery as previously described7,8 ...
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bioRxiv - Microbiology 2020Quote: ... Detection was carried out by incubating membranes with ECL Prime Western Blotting System (Sigma) and GE healthcare Amersham Hyperfilm ECL (GE) ...
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bioRxiv - Cell Biology 2019Quote: ... The “Spots” detection tool in Imaris was used to identify foci from C2 (Sigma) immunostaining ...
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bioRxiv - Neuroscience 2020Quote: ... and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP Substrate; Millipore, Cat# WBWF0500). The optical density of the relevant immunoreactive bands was measured after acquisition on a ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescent detection was performed with Luminata Classico Western HRP substrate (Millipore Sigma, Burlington, MA). Bio-Rad Chemidoc multiplex imaging system was used to detect and quantify the protein levels ...
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bioRxiv - Neuroscience 2020Quote: ... bioluminescent detection was performed using Immobilon Western HRP substrate (Merck Millipore WBKLS0500, 30 sec) and a Chemidoc Touch imager (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoreactive proteins were visualized using an enhanced chemiluminescence detection system (Millipore, Bedford, MA, USA). The relative intensity of the blot was measured using the ImageJ software and was normalized by the intensity of β-actin blot.
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bioRxiv - Cancer Biology 2022Quote: ... Reagents are from Duolink in Situ Detection Reagents Green assays (DUO92014, Sigma-ALdrich, USA). Samples were washed twice with buffer A ...
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bioRxiv - Cell Biology 2021Quote: Commercial primary antibodies were purchased for the detection of TIGAR (AB10545, Millipore/Sigma-Aldrich), ChAT (703789 ...