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Citations for Millipore Sigma :
1051 - 1100 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Blood DNA sample was prepared using Extract-N-Amp Blood PCR Kit (XNAB2R, Sigma, St. Louis, MO) and diluted 100 times ...
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Developmental Biology 2019Quote: ... in 50 mM ammonium bicarbonate (09830, Sigma-Aldrich; 30 min, RT), and alkylated in 50mM iodoacetamide (90034 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were permeabilized at RT in 0.5% Triton X-100 (Sigma), followed by 1 hr RT blocking in 2% BSA 0.5% Triton X-100 and overnight incubation with primary antibody.
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bioRxiv - Cell Biology 2020Quote: ... were incubated 10 minutes at RT with Bind-Silane (Sigma-Aldrich) dissolved in ethanol absolute (PanReac ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from A549 cells using RNAzol RT (Sigma-Aldrich) following the manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Biophysics 2021Quote: ... cells were incubated 10 min at RT with 1% glycine (Sigma). Cells were permeabilized by 0.1% saponin (Rectapur Prolabo ...
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bioRxiv - Microbiology 2019Quote: ... sodium azide (50 µg/ml, RT for 30 min, Sigma #S2002) for ATPase inhibition or chloramphenicol (30 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma, St. Louis, MO) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Genomics 2020Quote: ... carefully layered on an RT Ficoll gradient (Sigma HISTOPAQUE®-1119), and centrifuged at 400 x g for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... RT SigmaFast O-phenylenediamine (OPD) substrate (P9187, Sigma, St. Louis, MO) was added to the wells after six additional washes to develop the colorimetric reaction ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at RT and with DAPI (1/20,000; Sigma) for 5 min at RT ...
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bioRxiv - Immunology 2022Quote: ... for 2 hours at RT and then mounted with Fluoromount (Sigma). Primary Antibodies used were rabbit anti-ACE2 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 hr at RT in 0.1 % Calcofluor White (910090, Sigma-Aldrich), and (3 ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated using RNAzol® RT (Sigma-Aldrich, R4533) from three biological replicates of WT ...
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bioRxiv - Bioengineering 2022Quote: ... pressure-sensitive foils (Z734438, ThermalSeal RTS™ Sealing Films, Sigma-Aldrich) with holes for air exchange and to enable access to the reservoirs for medium exchange ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR primers were ordered as dried pellets from Sigma-Aldrich. The oligonucleotides were dissolved in 1X TE pH 8.0 to a final concentration of 100 μM and stored at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... all slices were incubated 20 min at RT with DAPI (Sigma). Finally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for 5 min at RT and 200 µL chloroform (Sigma-Aldrich) was added ...
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bioRxiv - Microbiology 2023Quote: ... nuclei were stained with DAPI (1:20,000, 10min, RT; Sigma Aldrich) or SiR-DNA dye (1 µM ...
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bioRxiv - Cell Biology 2024Quote: ... cells incubated for 1 h at RT in 5% BSA (Sigma) (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated for 1 h at RT in 50% formamide (Millipore). Cells were rewashed in 2xSSC ...
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bioRxiv - Immunology 2024Quote: ... 0.2ul ThermaStop™-RT Additive (Sigma-Aldrich, Catalog No. TSTOPRT-250), 0.5ul of sgE assay mix ...
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bioRxiv - Immunology 2019Quote: ... 3 times with 0.05% Tween 20 (Sigma) in PBS (PBS-T) ...
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bioRxiv - Genetics 2020Quote: ... rinsed 3 times with PBS1X (Sigma, D8662) and blocked with 5% normal donkey serum (NDS ...
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bioRxiv - Genetics 2023Quote: ... washed 3 times in PBS 1X (Sigma) with 0,1% Tween20 (Sigma) ...
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bioRxiv - Immunology 2021Quote: Cytotoxicity was assessed via lactate dehydrogenase (LDH) concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit FITC (Cat. No. 88-8005-72) was used in combination with Propidium Iodide Solution (Sigma-Aldrich, Cat. No. P-4864-10mL) as described in manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... For genotyping PCR JumpStart RedTaq PCR master mix (Sigma-Aldrich) was used following the manufacturer’s protocol for cycling with an annealing temperature of 55°C and 35 cycles.
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was isolated from 27 samples (‘EMEK3’, R bulk and S bulk × 3 treatments/ sampling time × 3 replicates) using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... 48 and 81) from ten time points during autumn (225-270 DOY) 2018 using Spectrum™ Plant Total RNA isolation kit (Sigma-Aldrich) according to manufacturer’s protocol (n=3 individual trees per genotype and time point) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (4 times for 20 s each time) and lysozyme (1 mg/ml; Sigma-Aldrich) in 50 mM Na phosphate ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit Q-PCR (Sigma, Cat#RTN350), and reverse transcription was performed using an oligoDT primer using superscript II (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: Genotyping to determine p53-/- mice was performed with the REDExtract-N-AMP Tissue PCR Kit (Sigma XNAT-100RXN). Ear punches were digested according to the kit protocol ...
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bioRxiv - Zoology 2021Quote: ... Amplified PCR products were gel-extracted and purified with the Illustra Exo ProStar kit (Sigma Aldrich, Madrid, Spain) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mice DNA was extracted from ear notches or tails using the RED ExtractN-Amp Tissue PCR Kit (SIGMA). For Lrp1flox ...
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Pathology 2022Quote: ... Target proteins were detected using ECL western blot detection solution (Millipore, WBKLS0500). Primary antibodies used for immunoblotting were as follows ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
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bioRxiv - Immunology 2019Quote: ... Colour detection was developed using ABTS or TMB liquid substrate (Sigma-Aldrich), which was subsequently stopped using 1% SDS (for ABTS ...
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bioRxiv - Microbiology 2021Quote: ... Detection was performed with anti-rabbit IgG-peroxydase antibodies (A0545, Sigma-Aldrich). Immunoblots are revealed with Pierce SuperSignal West Pico Chemiluminescent substrates (ThermoScientific).
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bioRxiv - Cell Biology 2020Quote: ... The membrane blots were developed with enhanced chemiluminescence (ECL) detection reagent (Millipore) per the manufacturer’s protocol and detected through chemiluminescence using ImageQuant LAS 400 Phospho-Imager (GE Healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... Chemiluminescent detection was finally performed using Immobilon Western HRP substrate (Millipore-Sigma) and captured using the Fusion FX imaging system (Vilber) ...
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bioRxiv - Developmental Biology 2022Quote: ... Chemiluminescent detection was finally performed using Immobilon Western HRP substrate (Millipore-Sigma) and captured using the Fusion FX imaging system (Vilber) ...
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bioRxiv - Cancer Biology 2022Quote: ... MPST detection was achieved using immunoblotting with anti-MPST (Sigma, 1:4000). The secondary antibody was anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Detection of polysialated NCAM (PSA-NCAM) was by clone 2-2B (Millipore). Membranes were washed with 0.5M NaCl in PBS 0.05% Tween 20 ...
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bioRxiv - Cell Biology 2020Quote: ... Bands were visualized using enhanced chemiluminescence detection reagents (Millipore, BioRad, and Ozyme) and autoradiographic films (Blue Devil ...