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Citations for Millipore Sigma :
951 - 1000 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... following detection using p-nitrophenyl phosphate (pNPP) substrate (Sigma, Israel).
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bioRxiv - Immunology 2021Quote: ... Chemiluminescent detection of blots was conducted with Luminata Forte (Millipore) in a G-box illuminator with Epi Red and FRLP filters (Syngene ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were developed using ECL Western Blotting Detection Reagent (Sigma) and exposed onto X-ray film.
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bioRxiv - Biophysics 2022Quote: ... To analyse the detection capabilities Tartrazine dilutions (Sigma Aldrich, USA) were used and for the deflection characterization experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Amplification was performed using Duolink FarRed detection reagents (Sigma DUO92013). Post-amplified samples were washed and incubated with alpha-tubulin antibody (DM1A ...
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bioRxiv - Microbiology 2023Quote: ... ROS detection was performed through dihydrorhodamine 123 (DHR123, Sigma‒ Aldrich). One microliter of DHR123 was added to a 1 mL cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated and amplified using red detection reagents (Sigma-Aldrich, DUO92008).
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bioRxiv - Bioengineering 2024Quote: ... and the chromogenic substrate used for detection is TMB (Sigma). Before quantification ...
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bioRxiv - Immunology 2022Quote: ... followed by chemiluminescence detection (Immobilon® Crescendo HRP Substrate, Millipore) with the ImageQuant LAS 4000 (GE Healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... The detection solution was HRP Immobilon Western Substrate (Merck Millipore). The analysis was performed using ANTI-FLAG® M2-Peroxidase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... and ECL Western Blotting Detection reagent (Millipore/Sigma, cat. # GERPN2106).
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bioRxiv - Neuroscience 2023Quote: ... and ECL Western Blotting Detection reagent (Millipore/Sigma, cat. # GERPN2106).
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bioRxiv - Cell Biology 2023Quote: ... and Duolink In Situ Detection Reagents Orange (DUO92007, Sigma-Aldrich). Cells were seeded on 12 mm poly-L-lysine (Y1251-100G ...
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bioRxiv - Immunology 2023Quote: ... the proteins were visualized using a chemiluminescence detection reagent (Millipore) or directly by fluorescence (LICOR) ...
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bioRxiv - Molecular Biology 2023Quote: ... For detection of Actin rabbit anti-Actin antibody (Sigma, A2066) was used at 1:2,000 dilution ...
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bioRxiv - Physiology 2019Quote: Fragmented DNA was stained by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) – method using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit S7100 (Merck Millipore SAS, Molsheim, France) according to the manufactureŕs instructions (Fig 2 B) ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay was carried out using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Millipore Sigma, St. Louis, MO); diaminobenzidine (DAB ...
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bioRxiv - Plant Biology 2019Quote: ... In situ PLA detection was carried out using the appropriate DUOLINK in situ Orange Kit Goat/Rabbit obtained from Sigma-Aldrich (Saint Luis, USA) according to the protocol of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... determined as the amount of 5-bromo-2′-deoxy-uridine (BrdU) incorporation (Roche BrDU Labeling and Detection Kit II, Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Neuroscience 2023Quote: The TUNEL assay was performed on paraffin-embedded sections and fifty-micron-thick free-floating frozen sections according to the manufacturer’s instructions but with the time of some steps lengthened to ensure good penetration of the reagents into the tissue (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Millipore-Sigma, Cat # S7110). Sections were post-fixed for ten minutes at room temperature in 2:1 ethanol:acetic acid ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... After 9 days 3-5 × 106 cells from selection conditions and 100 × 106 non-selection conditions were washed 3 times with PBS and pelleted by centrifugation for genomic DNA extraction using GeneElute Mammalian Genomic DNA Miniprep kit (Sigma) and Quick-DNA Midiprep Plus kit (Zymo Research ...
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bioRxiv - Physiology 2020Quote: ... tail blood from mice on CD or following HFD was collected at indicated time and plasma was frozen until assay for total GLP-1 (Multi Species GLP-1 Total ELISA kit, Millipore) or GIP (Mouse GIP ELISA Kit ...
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bioRxiv - Plant Biology 2021Quote: ... RNA was isolated at different time points using Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). Aliquots with 50 ng RNA were subjected to DNAse treatment (Thermo Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... The amount of glucose remaining at each time point was measured by using a glucose-oxidase-peroxidase reaction kit (Sigma). Glucose phosphorylation activity was evaluated from the total amount of glucose consumed per unit of time.
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bioRxiv - Microbiology 2021Quote: ... and stained for 5 minutes at RT with Giemsa (Sigma) diluted 1:1 in deionized water ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 5 minutes at RT with Giemsa (Sigma) diluted 1:1 in deionized water ...
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bioRxiv - Pathology 2022Quote: ... permeabilized (0.1% Triton X-100, Sigma, in PBS, 20min., RT), blocked with 2% goat serum (Life technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using RNAzol®RT (Sigma-Aldrich) and 2 µg of total RNA was subjected to reverse transcription using qScript cDNA Synthesis Kit (Quanta) ...
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bioRxiv - Developmental Biology 2021Quote: ... or Kicqstart One-Step Probe RT-qPCR ReadyMix (KCQS07; Sigma). Gli1 (Wen et al. ...
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bioRxiv - Cell Biology 2022Quote: ... RT and alkylated using 5.5 mM of iodoacetamide (Sigma-Aldrich) for 45 min ...
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bioRxiv - Immunology 2022Quote: ... fixed cells were blocked at RT with 1% BSA (Sigma) in PBS for 30 min and permeabilized with 0.1% Triton-X100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated for 1h at RT and DAPI (Sigma-Aldrich) was added for 10min to counterstain nuclei ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at RT and 70% isopropanol (Sigma-Aldrich) was added for 5 min ...
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bioRxiv - Genetics 2023Quote: ... and 1 volume of chloroform (2 min, RT, Sigma Aldrich), the aqueous phase was recovered after centrifugation at 12 000 g ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was isolated using RNAzol®RT (Sigma- Aldrich) as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using RNazol RT reagent (Sigma-Aldrich) using the protocol described previously (71) ...
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bioRxiv - Cell Biology 2022Quote: ... The amplified DNA was cleaned using a GenElute 96 Well PCR Clean-up kit (Sigma-Aldrich) and sent for sequencing.
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Physiology 2020Quote: ... Genotypes were confirmed from ear notches by using REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich) and primers as previously described12 ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic DNA extracted from the whole embryos (using Extract-N-Amp™ Tissue PCR Kit, Sigma) was used for the genotyping after vasculature imaging.
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bioRxiv - Physiology 2022Quote: ... Total genomic DNA from blood was isolated using REDExtract-N-Amp Blood PCR Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from 6 embryos using the Extract-N-Amp Tissue PCR Kit (Millipore-Sigma) and amplified using Amplitaq Gold Taq Polymerase (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture consisted of Kappa Probe Fast qPCR Master Mix (2X) Kit (Sigma-Aldrich), 0.75 μL of primers and 2.5 μL of RNA in a final volume of 10 μL reaction ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted using the RED Extract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO) according to the protocol of Shazib et al ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were incubated with the detection mix solution containing Texas Red-labeled detection probes that recognize the amplified product (Olink Bioscience, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT followed by 1 h incubation at RT with rabbit anti-FLAG antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA) and mouse anti-SEC31A antibody (1:500 ...