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Citations for Millipore Sigma :
1351 - 1400 of 7955 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Plates were washed five times and OPD color reaction (Sigma) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... and subsequently washed 3 times with PBS 2% FBS (Sigma). pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Protein detection was performed using chemiluminescent substrate (ECL™ Western Blotting Reagents, Sigma-Aldrich, GERPN2106) with film exposure (HyBlot CL® Autoradiography Film ...
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bioRxiv - Molecular Biology 2021Quote: ... PLA was performed by using the DUOLINK ® In situ detection reagents Red (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Ligation and amplification steps were performed using Duolink® in situ Detection reagents Red (Sigma). Ligase was incubated for 30 minutes at 37ºC and washed with 0,01% PBS-tween ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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Mediobasal hypothalamic FKBP51 acts as a molecular switch linking autophagy to whole-body metabolismbioRxiv - Neuroscience 2021Quote: ... The immuno-reactive bands were visualized either using ECL detection reagent (Millipore, Billerica, MA, USA) or directly by excitation of the respective fluorophore ...
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bioRxiv - Neuroscience 2022Quote: Proximity ligation assay by indirect detection was done using Duolink reagents and instructions (Sigma-Aldrich) on rat brain cryosections (16 μm ...
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bioRxiv - Microbiology 2020Quote: ... detection was performed by the addition of o-phenylene-diamine dihydrochloride substrate SigmaFast (Sigma-Aldrich) and incubated for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and detection was performed with peroxidase-conjugated anti-rabbit IgG 1:5000 (Sigma, MO, USA).
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for STED detection were Abberior Goat-anti-Rabbit STAR RED (Sigma, 41699) and AffiniPure Fab Fragment Goat Anti-Mouse Alexa Fluor 594 (Jackson Immunoresearch ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Cancer Biology 2020Quote: ... The FLAG M2 antibody used for the detection of MetRSL274G protein was purchase from Sigma. ANL ...
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bioRxiv - Microbiology 2024Quote: ... Primary detection was performed using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000), anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... detection was performed by the addition of o-phenylene-diamine dihydrochloride substrate SigmaFast (Sigma-Aldrich) and incubated for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... PLA was performed using the Duolink® In Situ Detection Reagents Red (DUO92008, Sigma-Aldrich) using the anti-mouse MINUS (DUO92004 ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble and chromatin fractions were probed with anti-HA (12CA5, Sigma for H3 detection), anti-H3K4me3 (ab8580 ...
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A spatially resolved EGFR signaling model predicts the length scale of GAB1-SHP2 complex persistencebioRxiv - Systems Biology 2023Quote: ... and were used and detected using Duolink In Situ Far Red Detection Reagents (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... followed by detection with fluorescently labeled secondary antibodies were used: anti-mouse CF680 (Sigma #SAB460199), anti-rabbit CF770 (Sigma #SAB460215) ...
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bioRxiv - Cell Biology 2023Quote: ... Autoactivation of PARP14 was determined by immunoblotting with a MAR detection reagent (Millipore Sigma, MABE1076).
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bioRxiv - Physiology 2023Quote: ... The antibody-reactive bands were visualized using an enhanced chemiluminescence Western blotting detection system (Millipore).
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bioRxiv - Microbiology 2023Quote: ... The following antibodies or detection reagents were used: mouse anti-Flag (Sigma, F3165; 1/4000), streptavidin-Alexa Fluor™ 680 Conjugate (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were purified using a MultiScreen® Filter Plates PCR µ96 (Millipore) and sent for Sanger sequencing (Fasteris ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5% w/v Alizarin Red S (Sigma-Aldrich, St. Louis, USA, 10 min incubation, RT) in distilled water was used to stain the mineralized extracellular matrix (ECM) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked for 30 min at RT in 1% BSA (Millipore Sigma, #A-4503) in PBS ...
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bioRxiv - Immunology 2019Quote: ... Eluted A20 was stabilized by incubation with iodoacetamide (Sigma; 30 mM, 30 min at RT). The reaction was terminated by addition of an equivalent amount of β-mercaptoethanol ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was carried out as previously described [20] using rat-specific oligonucleotide primers (Sigma) or FAM-MGB Taqman probes (see Supp Table 1 for primer list) ...
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bioRxiv - Pathology 2019Quote: ... Cells were washed 3x 5min each at RT in PBS-T (0.1% Tween20 (Sigma P9416). Secondary was performed at RT in the dark for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... at RT under agitation followed by quenching by addition of 0.125 M Glycine (Sigma G8898) for 5 min under agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed for 1h at RT using 20% normal goat serum (S26, Sigma-Aldrich) in Tris-NaCl blocking buffer (TNB) ...
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bioRxiv - Neuroscience 2020Quote: ... then washed overnight at RT with PBS containing 1% (v/v) Triton-X 100 (Sigma) and then with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong Solution (Millipore #2504), blocked for 30 min in 5% BLOTTO ...
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bioRxiv - Cell Biology 2021Quote: Coverslips were coated at RT with 5ug/mL Poly-L-lysine solution (Sigma-Aldrich, P4707) for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: RT-qPCR was carried out using FastStart Universal SYBR Green Master Mix (Rox) (Sigma Aldrich) in an Applied Biosystems QuantStudio 6 ...
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bioRxiv - Microbiology 2020Quote: ... Mannose inhibition: biotinylated HHL was incubated with methyl mannose (200 mM; 30 min, RT; Sigma) prior to HHL staining.
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bioRxiv - Immunology 2021Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... washed with 3x PBS at RT and stained with alizarin red S (Sigma Aldrich, A5533) 1% in ddH2O ...
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bioRxiv - Neuroscience 2021Quote: ... for 1 h each) at RT and then incubated in 66% dichloromethane (DCM, Sigma Aldrich)/33% MetOH overnight ...
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bioRxiv - Immunology 2019Quote: ... RT overnight) and trimethylsilylation (20μl of N,O-bis(trimetylsilyl)trifluoroacetamide + 1% trimethylchlorosilane) (Sigma, 33148) for ≥ 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Blots were stripped for 15 min at RT in ReBlot Plus Strong solution (Millipore #2504), blocked for 30 min in 5% blotto ...
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Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibersbioRxiv - Cell Biology 2020Quote: ... followed by incubation for 30 min at RT with anti-laminin (1:100; Sigma L9393). Secondary AlexaFluor antibodies (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... in the medium for 10 min at RT and quenched with 2.5 M Glycine (Sigma) for 2 min at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... and cross-linked for 10 min at RT by the addition of formaldehyde (F1635, SIGMA) to a final concentration of 1.2% ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed at RT with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MO, USA) in PBS ...
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bioRxiv - Immunology 2021Quote: ... blocked for 1 hr at room temperature (RT) in 5% Skim Milk Powder (Sigma-Aldrich) in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... sections were subjected to 0.5 % hydrogen peroxide solution at RT for 20 mins (H1009, Sigma) in methanol to quench endogenous peroxidases ...
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bioRxiv - Developmental Biology 2022Quote: ... were fixed for 15 min at RT in 4% (w/v) paraformaldehyde (Sigma, Cat. #158127) and permeabilized with 0.1% Triton-X-100 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Splenic B cells and bone marrow tissue cells were treated with RNAzol RT (Sigma-Aldrich), and total RNA was isolated according to the manufacturer’s instruction and saved at -80°C.